LOCUS       pCMV-Hadh(mouse)-HA     4714 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032663
VERSION     V032663
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        27..330
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        331..534
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          672..768
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             832..858
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             886..1827
                     /label="Hadh(NM_008212)"
                     /note="Hadh(NM_008212)"
                     /gene="Hadh"
     polyA_signal    1853..1987
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(2098..2114)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    2122..2138
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(2146..2176)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    2191..2212
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(2500..3088)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(3259..4119)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(4120..4224)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     4698..4714
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat gtacccatac
      841 gatgttccag attacgctct tatggccatg gaggcccgaa ttcggatggc gttcgtgacc
      901 aggcaattct tgcgctccat gtcctcctct tcctctgcgt cggcggccgc caagaagatc
      961 ctgatcaagc atgtgaccgt catcggcggc gggctgatgg gcgccggcat cgcgcaggta
     1021 gctgcagcaa ctggccatac agtagtattg gtggaccaaa cggaagacat cctggcaaaa
     1081 tccaagaagg gaattgagga gagtctaaag aggatggcaa agaagaagtt cacagaaaac
     1141 cctaaggctg gagatgagtt tgtggagaag accctgagct gcctttcaac cagcaccgat
     1201 gcggcatccg tggtgcacag cacagacctg gtggtggagg ccattgtgga gaacctgaag
     1261 ctgaagaacg agctgttcca gaggctggac aagttcgctg cagaacacac catctttgcc
     1321 agcaacacgt cttctttgca gatcacaaac atagccaatg ccaccaccag acaagaccga
     1381 tttgctggcc tccacttttt caaccccgtg cctatgatga agcttgtgga ggtcatcaaa
     1441 acaccgatga ccagccagaa gacatttgaa tctctggtgg acttttgtaa aaccttagga
     1501 aagcatcctg tctcttgcaa ggacactcct ggattcatcg tgaaccgact cttggtgcca
     1561 tacctcattg aggccgtcag gctacacgag cgaggcgatg cgtctaagga agacatcgat
     1621 acagcgatga agctgggagc tgggtatccc atgggtccgt ttgagcttct tgactatgtt
     1681 ggactggata ctacaaagtt catcttggac gggtggcatg aaatggagcc agagaacccc
     1741 ttatttcagc ccagtccttc catgaataat ctggtggccc agaagaagct gggcaagaag
     1801 accggagaag gattttacaa atacaagtga ctcgaggtac cgcggccgcg gggatccaga
     1861 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     1921 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     1981 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     2041 ggttttttcg gatcctctag agtcgatctg caggcatgct agcttggcgt aatcatggtc
     2101 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     2161 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     2221 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     2281 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     2341 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     2401 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     2461 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     2521 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2581 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     2641 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     2701 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     2761 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     2821 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     2881 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     2941 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3001 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3061 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3121 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3181 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     3241 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     3301 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     3361 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     3421 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     3481 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     3541 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     3601 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     3661 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     3721 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     3781 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     3841 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     3901 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     3961 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     4021 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     4081 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     4141 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     4201 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     4261 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     4321 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     4381 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     4441 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     4501 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
     4561 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     4621 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     4681 ttcccagtca cgacgttgta aaacgacggc cagt
//
