LOCUS       V008410                 7858 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008410
VERSION     V008410
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7858)
  AUTHORS   Cook KM, Hilton ST, Mecinovic J, Motherwell WB, Figg WD, Schofield
            CJ.
  TITLE     Epidithiodiketopiperazines block the interaction between
            hypoxia-inducible factor-1alpha (HIF-1alpha) and p300 by a zinc
            ejection mechanism
  JOURNAL   J. Biol. Chem. 284 (39), 26831-26838 (2009)
   PUBMED   19589782
REFERENCE   2  (bases 1 to 7858)
  AUTHORS   Figg WD, Chau CH, Cook KM.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2009) NCI, NIH, 10 Center Drive, Bldg 10, Rm 5A01,
            Bethesda, MD 20892, USA
REFERENCE   3  (bases 1 to 7858)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7858)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Biol.
            Chem."; date: "2009"; volume: "284"; issue: "39"; pages:
            "26831-26838"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-JUL-2009) NCI, NIH, 10 Center Drive, Bldg 10, Rm 5A01, Bethesda,
            MD 20892, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     primer_bind     634..650
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        673..691
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     protein_bind    716..740
                     /gene="mutant version of attB"
                     /label="attB1"
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             743..3220
                     /gene="HIF1A"
                     /label="Hypoxia-inducible factor 1-alpha"
                     /note="Hypoxia-inducible factor 1-alpha from Homo sapiens.
                     Accession#: Q16665"
     protein_bind    complement(3219..3243)
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3263..3286
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     polyA_signal    3616..3840
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3886..4314
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4328..4657
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4724..5515
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5582..5630
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     promoter        5936..6040
                     /label="AmpR promoter"
     CDS             6041..6898
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7072..7660
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
      601 agcctaggcc gcgggacgga taacaatttc acacaggaaa cagctatgac cattaggcct
      661 ttgcaaaaag ctatttaggt gacactatag aaggtacgcc tgcaggtacc ggatcacaag
      721 tttgtacaaa aaagttggca ccatggaggg cgccggcggc gcgaacgaca agaaaaagat
      781 aagttctgaa cgtcgaaaag aaaagtctcg agatgcagcc agatctcggc gaagtaaaga
      841 atctgaagtt ttttatgagc ttgctcatca gttgccactt ccacataatg tgagttcgca
      901 tcttgataag gcctctgtga tgaggcttac catcagctat ttgcgtgtga ggaaacttct
      961 ggatgctggt gatttggata ttgaagatga catgaaagca cagatgaatt gcttttattt
     1021 gaaagccttg gatggttttg ttatggttct cacagatgat ggtgacatga tttacatttc
     1081 tgataatgtg aacaaataca tgggattaac tcagtttgaa ctaactggac acagtgtgtt
     1141 tgattttact catccatgtg accatgagga aatgagagaa atgcttacac acagaaatgg
     1201 ccttgtgaaa aagggtaaag aacaaaacac acagcgaagc ttttttctca gaatgaagtg
     1261 taccctaact agccgaggaa gaactatgaa cataaagtct gcaacatgga aggtattgca
     1321 ctgcacaggc cacattcacg tatatgatac caacagtaac caacctcagt gtgggtataa
     1381 gaaaccacct atgacctgct tggtgctgat ttgtgaaccc attcctcacc catcaaatat
     1441 tgaaattcct ttagatagca agactttcct cagtcgacac agcctggata tgaaattttc
     1501 ttattgtgat gaaagaatta ccgaattgat gggatatgag ccagaagaac ttttaggccg
     1561 ctcaatttat gaatattatc atgctttgga ctctgatcat ctgaccaaaa ctcatcatga
     1621 tatgtttact aaaggacaag tcaccacagg acagtacagg atgcttgcca aaagaggtgg
     1681 atatgtctgg gttgaaactc aagcaactgt catatataac accaagaatt ctcaaccaca
     1741 gtgcattgta tgtgtgaatt acgttgtgag tggtattatt cagcacgact tgattttctc
     1801 ccttcaacaa acagaatgtg tccttaaacc ggttgaatct tcagatatga aaatgactca
     1861 gctattcacc aaagttgaat cagaagatac aagtagcctc tttgacaaac ttaagaagga
     1921 acctgatgct ttaactttgc tggccccagc cgctggagac acaatcatat ctttagattt
     1981 tggcagcaac gacacagaaa ctgatgacca gcaacttgag gaagtaccat tatataatga
     2041 tgtaatgctc ccctcaccca acgaaaaatt acagaatata aatttggcaa tgtctccatt
     2101 acccaccgct gaaacgccaa agccacttcg aagtagtgct gaccctgcac tcaatcaaga
     2161 agttgcatta aaattagaac caaatccaga gtcactggaa ctttctttta ccatgcccca
     2221 gattcaggat cagacaccta gtccttccga tggaagcact agacaaagtt cacctgagcc
     2281 taatagtccc agtgaatatt gtttttatgt ggatagtgat atggtcaatg aattcaagtt
     2341 ggaattggta gaaaaacttt ttgctgaaga cacagaagca aagaacccat tttctactca
     2401 ggacacagat ttagacttgg agatgttagc tccctatatc ccaatggatg atgacttcca
     2461 gttacgttcc ttcgatcagt tgtcaccatt agaaagcagt tccgcaagcc ctgaaagcgc
     2521 aagtcctcaa agcacagtta cagtattcca gcagactcaa atacaagaac ctactgctaa
     2581 tgccaccact accactgcca ccactgatga attaaaaaca gtgacaaaag accgtatgga
     2641 agacattaaa atattgattg catctccatc tcctacccac atacataaag aaactactag
     2701 tgccacatca tcaccatata gagatactca aagtcggaca gcctcaccaa acagagcagg
     2761 aaaaggagtc atagaacaga cagaaaaatc tcatccaaga agccctaacg tgttatctgt
     2821 cgctttgagt caaagaacta cagttcctga ggaagaacta aatccaaaga tactagcttt
     2881 gcagaatgct cagagaaagc gaaaaatgga acatgatggt tcactttttc aagcagtagg
     2941 aattggaaca ttattacagc agccagacga tcatgcagct actacatcac tttcttggaa
     3001 acgtgtaaaa ggatgcaaat ctagtgaaca gaatggaatg gagcaaaaga caattatttt
     3061 aataccctct gatttagcat gtagactgct ggggcaatca atggatgaaa gtggattacc
     3121 acagctgacc agttatgatt gtgaagttaa tgctcctata caaggcagca gaaacctact
     3181 gcagggtgaa gaattactca gagctttgga tcaagttaac ccaactttct tgtacaaagt
     3241 ggttcgatct agaatggcta gcgactacaa ggacgacgat gacaagtagt aatcgagtac
     3301 aactataact cacacaatgt atacatcacg gcagacaaac aaaagaatgg aatcaaagct
     3361 aacttcaaaa ttcgccacaa cattgaagat ggatccgttc aactagcaga ccattatcaa
     3421 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtcgaca
     3481 caatctgccc tttcgaaaga tcccaacgaa aagcgtgacc acatggtcct tcttgagttt
     3541 gtaactgctg ctgggattac acatggcatg gatgagctct acaaataatg aattaaaccc
     3601 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     3661 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     3721 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     3781 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     3841 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     3901 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     3961 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     4021 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     4081 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     4141 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     4201 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     4261 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     4321 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4381 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     4441 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     4501 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4561 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     4621 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     4681 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     4741 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     4801 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     4861 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     4921 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     4981 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     5041 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     5101 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     5161 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     5221 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     5281 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     5341 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     5401 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     5461 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     5521 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgatggccg
     5581 caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagc
     5641 gataaggatc cgcgtatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     5701 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     5761 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     5821 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     5881 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     5941 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     6001 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     6061 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     6121 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     6181 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     6241 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     6301 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     6361 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     6421 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     6481 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     6541 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     6601 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     6661 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     6721 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     6781 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6841 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6901 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6961 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     7021 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     7081 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     7141 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     7201 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     7261 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     7321 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     7381 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     7441 actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc
     7501 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     7561 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7621 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7681 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     7741 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     7801 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagctcgcga atgcatgt
//
