LOCUS       V015063                 8514 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V015063
VERSION     V015063
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8514
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        50..429
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        430..633
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             648..1301
                     /label="GST"
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             1335..3224
                     /gene="ATG7"
                     /label="Ubiquitin-like modifier-activating enzyme ATG7"
                     /note="Ubiquitin-like modifier-activating enzyme ATG7 from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P38862"
     misc_feature    3290..3878
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    3944..3992
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4194..4622
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4636..4965
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5032..5823
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    6002..6135
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6172..6188)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6196..6212)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6220..6250)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6265..6286)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6574..7162)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7336..8193)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8194..8298)
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcccaccatg tcccctatac
      661 taggttattg gaaaattaag ggccttgtgc aacccactcg acttcttttg gaatatcttg
      721 aagaaaaata tgaagagcat ttgtatgagc gcgatgaagg tgataaatgg cgaaacaaaa
      781 agtttgaatt gggtttggag tttcccaatc ttccttatta tattgatggt gatgttaaat
      841 taacacagtc tatggccatc atacgttata tagctgacaa gcacaacatg ttgggtggtt
      901 gtccaaaaga gcgtgcagag atttcaatgc ttgaaggagc ggttttggat attagatacg
      961 gtgtttcgag aattgcatat agtaaagact ttgaaactct caaagttgat tttcttagca
     1021 agctacctga aatgctgaaa atgttcgaag atcgtttatg tcataaaaca tatttaaatg
     1081 gtgatcatgt aacccatcct gacttcatgt tgtatgacgc tcttgatgtt gttttataca
     1141 tggacccaat gtgcctggat gcgttcccaa aattagtttg ttttaaaaaa cgtattgaag
     1201 ctatcccaca aattgataag tacttgaaat ccagcaagta tatagcatgg cctttgcagg
     1261 gctggcaagc cacgtttggt ggtggcgacc atcctccaaa aggaggaggg ggaagcggcg
     1321 gcggagggag taagctttcg tcagaaaggg tcttaagtta tgcaccagct tttaaatcat
     1381 ttctggatac atcttttttc caagaattat cgagattgaa actcgatgtt ctgaaattag
     1441 attcaacatg ccaaccactt actgtgaatc tagatttaca caacattcct aaatctgctg
     1501 atcaagttcc attattctta acaaatagga gatttgaaaa acacaataat aagcgtacta
     1561 atgaagtgcc tttacagggc agtattttca attttaatgt attagacgag tttaagaatc
     1621 ttgataagca actattttta catcaaagag cattggaatg ctgggaagat ggaataaagg
     1681 atatcaataa gtgtgtttct tttgtgatta ttagttttgc tgacctaaaa aagtacaggt
     1741 tttattactg gttaggtgtt ccctgttttc aaagaccctc ttcaacagtt ttacatgtgc
     1801 ggccagagcc gagtttaaaa gggctgttct caaagtgtca aaaatggttt gatgtcaatt
     1861 actcaaaatg ggtgtgtata ctggatgcag acgatgaaat agtaaattat gacaagtgta
     1921 ttattcgaaa aactaaagtt ttggcgataa gggacactag tacgatggaa aacgtccctt
     1981 ctgctctaac caaaaatttt ctcagcgtgt tgcaatacga tgttcctgac ttgatagatt
     2041 tcaaactatt aattattaga cagaacgaag gtagctttgc attaaatgct acatttgctt
     2101 ctattgaccc acaatcatcc tcaagtaacc cagatatgaa agtttctgga tgggaaagga
     2161 atgtgcaagg taagctagca ccacgtgttg ttgatttaag ttcattgtta gatcctttaa
     2221 aaattgctga ccaatccgtg gatttaaatt tgaagctaat gaaatggaga attcttcctg
     2281 acttaaatct agatatcatc aaaaacacaa aagtactact actaggtgct ggtacactag
     2341 gttgttatgt ttcacgcgca ttgatagcat ggggggttag aaaaataaca tttgtggata
     2401 acggtacagt ttcatattca aatccagtga ggcaagcgtt atataatttt gaggactgtg
     2461 gaaagccaaa ggcagaactt gcggctgcgt ctttgaaaag aatatttcct ttaatggacg
     2521 ctactggtgt gaaattaagt attcctatga tcggtcataa attagtaaac gaggaggctc
     2581 agcataagga ttttgataga ttaagagcat taataaaaga gcatgatatc atttttttat
     2641 tggtggattc tcgagaaagc agatggcttc cgtcgttatt gagcaacatc gaaaacaaaa
     2701 ccgttattaa tgctgctctg gggtttgata gctacttagt tatgaggcat ggtaatagag
     2761 atgaacagtc ttcaaaacaa ctgggctgtt atttttgcca tgatgtggta gcaccaactg
     2821 acagtttaac tgacaggact ttggatcaaa tgtgcacagt aactagaccc ggcgttgcta
     2881 tgatggcctc ttctttagca gttgaattga tgacttcctt actacagacc aaatactctg
     2941 gttcagaaac aacagtatta ggggatatac ctcatcaaat acgtgggttt ttgcataatt
     3001 tttccatctt aaaattagaa actccagcct atgagcattg tccagcatgt agccctaaag
     3061 ttatcgaagc atttacagac ttaggttggg aatttgttaa aaaggccttg gagcatccac
     3121 tttaccttga agaaattagc ggtttgtcag tcataaagca ggaggtcgaa cgactaggca
     3181 acgatgtttt tgaatgggaa gatgatgaat ctgatgagat tgcttaagaa ttctgcagat
     3241 atccagcaca gtggcggccg ctcgagtcta gagggcccga tatctcgaca atcaacctct
     3301 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     3361 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     3421 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     3481 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     3541 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     3601 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     3661 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     3721 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     3781 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     3841 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaaacgggg gaggctaact
     3901 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     3961 aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
     4021 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
     4081 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
     4141 ggcaggccct gccatagcag atctgcgcag ctggggctct agggggtatc cccacgcgcc
     4201 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4261 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4321 cggctttccc cgtcaagctc taaatcgggg catcccttta gggttccgat ttagtgcttt
     4381 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     4441 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4501 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     4561 tttggggatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4621 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     4681 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     4741 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     4801 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     4861 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     4921 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     4981 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     5041 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     5101 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     5161 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     5221 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     5281 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     5341 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     5401 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     5461 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     5521 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     5581 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     5641 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     5701 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     5761 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     5821 ttctgagcgg gactctgggg ttcgcgaaat gaccgaccaa gcgacgccca acctgccatc
     5881 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     5941 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     6001 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6061 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6121 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     6181 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     6241 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     6301 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     6361 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     6421 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6481 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     6541 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6601 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6661 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6721 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg
     6781 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6841 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6901 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6961 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     7021 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     7081 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7141 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7201 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7261 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7321 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7381 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7441 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7501 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     7561 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     7621 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     7681 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     7741 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     7801 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     7861 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     7921 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     7981 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     8041 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     8101 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     8161 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     8221 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     8281 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     8341 agatctcccg atcccctatg gtcgactctc agtacaatct gctctgatgc cgcatagtta
     8401 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     8461 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagg
//
