LOCUS       V014110                 8234 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014110
VERSION     V014110
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8234)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        50..429
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        430..633
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             683..2881
                     /gene="FOXK1"
                     /label="Forkhead box protein K1"
                     /note="Forkhead box protein K1 from Homo sapiens.
                     Accession#: P85037"
     CDS             2921..2986
                     /label="3xFLAG"
                     /note="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
     misc_feature    3010..3598
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    3664..3712
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3914..4342
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4356..4685
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4752..5543
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5722..5855
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5892..5908)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5916..5932)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5940..5970)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5985..6006)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6294..6882)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7056..7913)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7914..8018)
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccgcca ccatggctga ggtgggagag gacagcggag ctagggctct
      721 gctggctctg aggagcgctc cttgcagccc tgtgctgtgc gctgctgctg ctgccgctgc
      781 tttccctgct gctgctcctc ctcctgctcc tgctcagcct cagcctcctc ctggacctcc
      841 tcctcctccc cctcctcctc tgcctcctgg agctatcgct ggagctggaa gcagcggagg
      901 aagcagcggc gtgagcggag acagcgctgt ggctggagct gctcctgctc tggtggctgc
      961 tgctgctgct agcgtgaggc agagccctgg acctgctctg gctaggctgg agggaaggga
     1021 gttcgagttc ctgatgaggc agcctagcgt gaccatcgga aggaacagca gccagggaag
     1081 cgtggacctg agcatgggac tgagcagctt catcagcagg aggcacctgc agctgagctt
     1141 ccaggagcct cacttctacc tgaggtgcct gggaaagaat ggagtgtttg tggacggagc
     1201 tttccagagg aggggagctc ctgctctgca gctgcctaag cagtgcacct tcaggttccc
     1261 tagcaccgct atcaaaatcc agttcaccag cctgtaccac aaggaagaag ctcctgcttc
     1321 tccactgaga cctctgtacc ctcagatttc tcctctgaag atccacatcc ccgagcctga
     1381 cctgaggagc atggtgagcc ctgtgcctag ccctaccgga accatcagcg tgcctaacag
     1441 ctgccctgct agccctaggg gagctggatc tagcagctac aggttcgtgc agaatgtgac
     1501 aagcgacctg cagctggctg ctgagttcgc tgctaaggct gctagcgagc agcaggctga
     1561 caccagcgga ggagacagcc ctaaggacga gagcaagcct cctttcagct atgctcagct
     1621 gatcgtgcag gctattagca gcgctcagga caggcagctg accctgagcg gaatctacgc
     1681 tcacatcacc aaacactacc cctactacag gaccgctgat aaaggatggc agaactctat
     1741 ccggcacaac ctgagcctga atagatactt catcaaggtg cctaggagcc aggaagaacc
     1801 tggaaaggga agcttttgga ggatcgatcc agcttctgag gctaaactgg tggaacaggc
     1861 tttcagaaag cggaggcaga ggggagtgag ctgcttcagg acccctttcg gacctctgag
     1921 cagcaggagc gctcccgcta gccctaccca ccctggactg atgagcccta ggagcggagg
     1981 actgcagacc cctgagtgcc tgagcaggga gggaagccct atccctcacg accctgagtt
     2041 cggaagcaag ctggctagcg tgcctgagta caggtacagc cagagcgctc ctggaagccc
     2101 tgtgagcgct cagcctgtga tcatggctgt gcctcctagg cctagcagcc tggtggctaa
     2161 gcctgtggct tacatgcctg ctagcattgt gacaagccag cagcctgctg gacacgctat
     2221 ccacgtggtg cagcaggctc ctaccgtgac catggtgagg gtggtgacca ccagcgctaa
     2281 cagcgctaac ggatacattc tgaccagcca gggagctgct ggaggaagcc acgacgctgc
     2341 tggagctgcc gtgctggacc tgggaagcga ggctagggga ctggaggaga agcctaccat
     2401 cgctttcgct accattcctg ctgctggagg cgtgatccag accgtggcta gccagatggc
     2461 tcctggagtg cctggacaca ccgtgaccat cctgcagcct gctacccctg tgaccctggg
     2521 acagcaccac ctgcctgtga gggctgtgac ccagaacgga aagcacgctg tgcctaccaa
     2581 cagcctggct ggaaacgctt acgctctgac cagccctctg cagctgctgg ctacccaggc
     2641 tagcagcagc gctcctgtgg tggtgaccag ggtgtgcgag gtgggaccta aggagcctgc
     2701 tgctgccgtg gctgctaccg ctaccaccac ccctgctacc gctacaaccg ctagcgctag
     2761 cgcttccagc accggagagc ctgaggtgaa gaggagcagg gtggaggagc ctagcggagc
     2821 tgtgaccacc cctgccggag tgatcgctgc tgctggacct cagggacctg gaaccggaga
     2881 ggagttctgc aggtaccctg ctcagtggag gcctctcgag gactacaaag accatgacgg
     2941 tgattataaa gatcatgaca tcgactacaa ggatgacgat gacaagtagt gagggcccga
     3001 tatctcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     3061 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     3121 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     3181 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     3241 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     3301 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     3361 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     3421 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     3481 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     3541 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     3601 ggaaacgggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
     3661 tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc
     3721 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
     3781 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
     3841 ctcgcagcca acgtcggggc ggcaggccct gccatagcag atctgcgcag ctggggctct
     3901 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     3961 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4021 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta
     4081 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4141 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4201 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     4261 tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt
     4321 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     4381 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     4441 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4501 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     4561 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     4621 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     4681 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag
     4741 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     4801 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     4861 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     4921 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     4981 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     5041 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     5101 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     5161 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     5221 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     5281 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     5341 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     5401 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     5461 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     5521 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat gaccgaccaa
     5581 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     5641 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     5701 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     5761 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     5821 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     5881 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     5941 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     6001 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     6061 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     6121 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     6181 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     6241 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6301 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     6361 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     6421 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     6481 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     6541 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     6601 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     6661 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     6721 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     6781 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     6841 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     6901 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     6961 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     7021 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     7081 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     7141 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     7201 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     7261 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     7321 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     7381 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     7441 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     7501 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     7561 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     7621 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     7681 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     7741 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     7801 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     7861 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     7921 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     7981 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     8041 cctgacgtcg acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct
     8101 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     8161 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     8221 agaatctgct tagg
//
