LOCUS       62056_6930        5495 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5495)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..330
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        331..534
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          672..768
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             832..855
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     polyA_signal    complement(2634..2768)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2879..2895)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2903..2919)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2927..2957)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2972..2993)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3281..3869)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4043..4900)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4901..5005)
                     /label=AmpR promoter
     primer_bind     5479..5495
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat ggactacaag
      841 gacgacgatg acaagcttat gaaaatgatg cgatgtgtcc gatgccccgt tgcctatcat
      901 ggaggggatg cctgtctggc agcaggatgt tcggtgattg cttctaacag cataatctgc
      961 acaggccact tcactgcccg gaaggggaag cggcaccata cccatgtcaa tgtgagctgg
     1021 tgctttgtgt gctccaaagg gggaagcctt ctgtgctgtg aggcctgccc agcagccttt
     1081 caccccgatt gtctaaacat cgagatgccc gatggtagct ggttctgcaa cgattgccgg
     1141 gctggaaaga agctccactt tcaagacatc atctgggtca aacttggtaa ctacagatgg
     1201 tggccggcag aagtttgcca tcccaaaaat gttcccccaa atattcagaa aatgaagcac
     1261 gagattggag aattccctgt atttttcttt gggtctaaag attattactg gacgcatcag
     1321 gcacgagtgt tcccatacat ggagggggac cggggcagcc gctaccaggg ggtcagaggg
     1381 atcggaagag tcttcaagaa cgcattgcaa gaagctgaag ctcgttttaa tgaagtcaaa
     1441 cttcagcggg aagcccgaga aacacaggag agtgagagaa agcctccacc atacaagcat
     1501 atcaaggtga ataaacctta tggtaaagtc cagatctaca cagctgacat ttctgaaatc
     1561 ccgaagtgca actgtaagcc cacagatgag aacccctgtg gctctgattc agagtgtctg
     1621 aacaggatgc taatgtttga gtgccacccg caggtgtgtc ctgcagggga atactgccag
     1681 aaccagtgtt tcactaagcg ccagtaccct gagaccaaga tcatcaagac agatggcaaa
     1741 gggtggggcc tggttgccaa aagggacatc agaaaaggag aatttgttaa tgagtatgtt
     1801 ggtgagctga ttgatgaaga ggagtgtatg gcaagaatca aatatgcaca tgagaatgac
     1861 atcactcact tctacatgct caccatagac aaggaccgca taattgatgc tggccccaaa
     1921 gggaattatt cacgattcat gaaccatagc tgccagccca attgtgagac cctgaagtgg
     1981 acagtgaatg gggacacacg ggttggcctg tttgctgtgt gtgacattcc tgcaggtaca
     2041 gagctgactt tcaactataa ccttgattgt ttgggcaatg aaaagacagt ctgtcggtgt
     2101 ggagcctcca actgtagtgg gttccttgga gacagaccaa agacatcagc atccctttca
     2161 tcagaggaaa agggtaaaaa ggccaagaag aaaaccagaa ggcgcagagc caagggtgaa
     2221 ggaaaacggc agtcagagga tgagtgcttc cgctgtggtg atggtgggca gctggtgctg
     2281 tgtgaccgca agttctgtac caaggcctac cacctatcct gcctcggttt gggcaagcgg
     2341 ccctttggga agtgggaatg tccttggcat cactgtgatg tatgtggcaa accttctacc
     2401 tcattttgcc acctctgccc caactcattc tgtaaggaac accaagatgg gactgctttc
     2461 cgttccaccc aggatgggca gtcctactgc tgtgagcatg acttgagggc agactcttca
     2521 agtagcacca agactgagaa accctttcca gaatcactga agtcaaaagg aaagaggaag
     2581 aaaaggcggt gctggcgaag ggtcacagat ggctgaggta ccgcggccgc ggggatccag
     2641 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     2701 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     2761 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     2821 aggttttttc ggatcctcta gagtcgatct gcaggcatgc tagcttggcg taatcatggt
     2881 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     2941 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     3001 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     3061 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     3121 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     3181 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     3241 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     3301 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     3361 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     3421 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     3481 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     3541 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     3601 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     3661 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     3721 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     3781 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     3841 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     3901 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     3961 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     4021 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     4081 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     4141 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     4201 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     4261 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     4321 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     4381 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     4441 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     4501 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     4561 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     4621 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     4681 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     4741 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     4801 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     4861 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     4921 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     4981 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     5041 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     5101 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     5161 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     5221 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     5281 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     5341 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     5401 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     5461 tttcccagtc acgacgttgt aaaacgacgg ccagt
//
