LOCUS       V032821                 4943 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032821
VERSION     V032821
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        27..330
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        331..534
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          672..768
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             832..858
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             886..2067
                     /label="Ergic3(NM_001356413)"
                     /note="Ergic3(NM_001356413)"
                     /gene="Ergic3"
     polyA_signal    2082..2216
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(2327..2343)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    2351..2367
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(2375..2405)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    2420..2441
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(2729..3317)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(3488..4348)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(4349..4453)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     4927..4943
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat gtacccatac
      841 gatgttccag attacgctct tatggccatg gaggcccgaa ttcggatgga ggcgctgggg
      901 aagctgaagc agttcgatgc ctaccctaag actctggagg acttccgggt caagacctgc
      961 gggggtgcca cggtgaccat cgtcagtggc cttctcatgc tcctgctttt cctatcggag
     1021 ttgcagtatt atctcactac ggaggtgcat cctgagctct acgtggacaa gtctcggggg
     1081 gataaactga agatcaacat cgatgttctt ttcccgcaca tgccttgtgc ctacttgagc
     1141 atcgatgcca tggacgtggc cggggagcag cagctggatg tggaacacaa cctgttcaag
     1201 aaacgactag acaaggatgg cgtccccgtg agctcagagg ctgaacggca cgagcttggg
     1261 aaagtcgagg tgacagtgtt tgaccccaac tccttggacc ccaatcgctg tgagagctgc
     1321 tacggcgctg agtcagaaga catcaagtgc tgtaacagtt gtgaagatgt gcgggaggcc
     1381 tatcgccgtc gaggctgggc cttcaagaac ccggacacca ttgagcagtg tcggcgagag
     1441 ggcttcagcc agaagatgca ggagcagaag aatgaaggct gccaggtgta cggcttcttg
     1501 gaggtcaaca aggtggctgg aaacttccac tttgcccctg ggaagagctt ccaacagtct
     1561 catgtgcatg tccatgctgt ggagattcat gacttgcaga gctttggcct tgacaatcca
     1621 tctgattgct tgcagatcaa catgacacac tacatcaagc acctgtcatt cggggaggac
     1681 tatccaggaa tcgtgaaccc cctagaccac accaacgtga ctgcacccca agcctccatg
     1741 atgttccagt actttgtgaa ggtggtgccc acagtgtaca tgaaggtgga cggggaggtg
     1801 ctgaggacta accagttctc cgtgaccagg cacgagaagg ttgccaatgg gctcctgggt
     1861 gaccagggcc tgccgggggt ctttgtgctg tatgagctct caccaatgat ggtgaagctg
     1921 acggagaaac acaggtcctt cacccacttt ctgacaggtg tgtgcgccat cattgggggc
     1981 atgtttacag tggccggcct cattgactcg ctcatctacc actccgctcg ggccatccag
     2041 aagaaaattg acctagggaa gacaacgtag gcggccgcgg ggatccagac atgataagat
     2101 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     2161 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     2221 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttcgg
     2281 atcctctaga gtcgatctgc aggcatgcta gcttggcgta atcatggtca tagctgtttc
     2341 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     2401 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     2461 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     2521 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     2581 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     2641 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     2701 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2761 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2821 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2881 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2941 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3001 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     3061 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     3121 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     3181 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     3241 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     3301 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3361 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     3421 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     3481 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     3541 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     3601 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     3661 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     3721 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     3781 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3841 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     3901 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     3961 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     4021 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     4081 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     4141 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     4201 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     4261 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     4321 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     4381 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     4441 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     4501 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     4561 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     4621 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     4681 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     4741 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
     4801 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     4861 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     4921 gacgttgtaa aacgacggcc agt
//
