LOCUS       V014132                 6602 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014132
VERSION     V014132
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6602)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             613..1329
                     /label="EGFP"
                     /note="enhanced GFP"
     CDS             1354..3219
                     /gene="ZDHHC13"
                     /label="Palmitoyltransferase ZDHHC13"
                     /note="Palmitoyltransferase ZDHHC13 from Homo sapiens.
                     Accession#: Q8IUH4"
     polyA_signal    3390..3511
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3518..3973)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4000..4104
                     /label="AmpR promoter"
     promoter        4106..4463
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4498..5289
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5524..5571
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5900..6488
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caaatggagg ggccggggct gggctcgcag
     1381 tgcaggaatc acagccatgg cccccaccct ccaggatttg gtcgatatgg catctgtgca
     1441 catgaaaaca aagaacttgc caatgcaaga gaagctcttc ctcttataga ggactctagt
     1501 aactgtgaca ttgtcaaagc tactcaatac ggaatttttg aacgatgtaa agagttggta
     1561 gaagcaggat atgatgtcag gcaaccagat aaagaaaatg tgtcgcttct tcattgggct
     1621 gctattaaca acagactgga tcttgtaaag ttttatattt caaaaggtgc tgttgtagat
     1681 cagttgggtg gagatttaaa ttcaactcct cttcactggg ccatccgaca aggacattta
     1741 cctatggtca tattattact ccagcatggt gcagacccca ctcttattga tggagaggga
     1801 ttcagcagca tccacctggc agtattgttt caacacatgc ctattatagc atatctcatc
     1861 tcaaagggac agagtgtgaa tatgacagat gtaaatgggc agacacctct catgttatca
     1921 gctcacaaag taattgggcc agaaccaact ggatttcttt taaagtttaa tccttctctc
     1981 aatgtggttg ataaaataca ccaaaacact ccacttcact gggcagttgc agcaggaaat
     2041 gttaatgcag ttgataagct tttggaagct ggttctagcc tggatatcca gaatgttaag
     2101 ggagaaacac ctcttgatat ggctctacaa aacaaaaatc agctcattat tcatatgcta
     2161 aaaacagaag ccaaaatgag agccaaccaa aagttcagac tttggaggtg gctgcagaaa
     2221 tgcgagctct tcctgctgct gatgctttct gtgattacca tgtgggctat tggatacata
     2281 ttggacttca attcagattc ttggctttta aaaggatgtc ttctagtaac actgtttttt
     2341 ctgacatctt tgtttccaag gttcttggtt gggtataaga accttgtata cttaccaaca
     2401 gcctttctgc taagttctgt tttttggata tttatgactt ggttcatctt attttttcct
     2461 gatttagcag gagccccttt ctatttcagt ttcattttca gcatagtagc ctttctatac
     2521 tttttctata agacttgggc aactgatcca ggcttcacta aggcttctga agaagaaaag
     2581 aaagtgaata tcatcaccct tgcagaaact ggctctctgg acttcagaac attttgtaca
     2641 tcatgtctta taaggaagcc attaaggtca ctccactgcc atgtatgcaa ctgctgtgtg
     2701 gctcgatatg atcaacactg cctgtggact ggacggtgca taggttttgg caaccatcac
     2761 tattacatat tcttcttgtt tttcctttcc atggtatgtg gctggattat atatggatct
     2821 ttcatctatt tgtccagtca ttgtgccaca acattcaaag aagatggatt atggacttac
     2881 ctcaatcaga ttgtggcctg ttccccttgg gttttatata tcttgatgct agcaactttc
     2941 catttctcat ggtcaacatt tttattatta aatcaactct ttcagattgc ctttctgggc
     3001 ctgacctccc atgagagaat cagcctgcag aagcagagca agcatatgaa acagacgttg
     3061 tccctcagga agacaccata caatcttgga ttcatgcaga acctggcaga tttctttcag
     3121 tgtggctgct ttggcttggt gaagccctgt gtggtagatt ggacatcaca gtacaccatg
     3181 gtctttcacc cagccaggga gaaggttctt cgctcagtat gaaagcttcg aattctgcag
     3241 tcgacggtac cgcgggcccg ggatccaccg gatctagata actgatcata atcagccata
     3301 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     3361 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     3421 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     3481 gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg
     3541 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     3601 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     3661 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     3721 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     3781 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     3841 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     3901 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     3961 ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4021 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     4081 caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc
     4141 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4201 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4261 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     4321 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     4381 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     4441 ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg
     4501 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     4561 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     4621 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
     4681 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     4741 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     4801 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     4861 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     4921 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     4981 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
     5041 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     5101 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     5161 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     5221 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     5281 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     5341 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     5401 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     5461 accctagggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
     5521 tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg
     5581 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
     5641 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
     5701 tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt
     5761 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     5821 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     5881 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     5941 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6001 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     6061 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6121 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6181 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6241 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6301 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6361 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6421 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6481 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6541 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc
     6601 at
//
