LOCUS       V014139                 6462 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014139
VERSION     V014139
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             613..1329
                     /label="EBFP"
                     /note="enhanced blue variant of GFP"
     CDS             1369..3117
                     /gene="gox"
                     /label="Glucose oxidase"
                     /note="Glucose oxidase from Aspergillus niger. Accession#:
                     P13006"
     polyA_signal    3250..3371
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3378..3833)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3860..3964
                     /label="AmpR promoter"
     promoter        3966..4323
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4358..5149
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5384..5431
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5760..6348
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc cacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaacttc aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagga ggagggggaa gcgaattcag caacggcatc
     1381 gaggccagcc tgctgaccga ccccaaggac gtgagcggcc gcaccgtgga ctacatcatc
     1441 gccggcggcg gcctgaccgg cctgaccacc gccgcccgcc tgaccgagaa ccccaacatc
     1501 agcgtgctgg tgatcgagag cggcagctac gagagcgacc gcggccccat catcgaggac
     1561 ctgaacgcct acggcgacat cttcggcagc agcgtggacc acgcctacga gaccgtggag
     1621 ctggccacca acaaccagac cgccctgatc cgcagcggca acggcctggg cggcagcacc
     1681 ctggtgaacg gcggcacctg gacccgcccc cacaaggccc aggtggacag ctgggagacc
     1741 gtgttcggca acgagggctg gaactgggac aacgtggccg cctacagcct gcaggccgag
     1801 cgcgcccgcg cccccaacgc caagcagatc gccgccggcc actacttcaa cgccagctgc
     1861 cacggcgtga acggcaccgt gcacgccggc ccccgcgaca ccggcgacga ctacagcccc
     1921 atcgtgaagg ccctgatgag cgccgtggag gaccgcggcg tgcccaccaa gaaggacttc
     1981 ggctgcggcg acccccacgg cgtgagcatg ttccccaaca ccctgcacga ggaccaggtg
     2041 cgcagcgacg ccgcccgcga gtggctgctg cccaactacc agcgccccaa cctgcaggtg
     2101 ctgaccggcc agtacgtggg caaggtgctg ctgagccaga acggcaccac cccccgcgcc
     2161 gtgggcgtgg agttcggcac ccacaagggc aacacccaca acgtgtacgc caagcacgag
     2221 gtgctgctgg ccgccggcag cgccgtgagc cccaccatcc tggagtacag cggcatcggc
     2281 atgaagagca tcctggagcc cctgggcatc gacaccgtgg tggacctgcc cgtgggcctg
     2341 aacctgcagg accagaccac cgccaccgtg cgcagccgca tcaccagcgc cggcgccggc
     2401 cagggccagg ccgcctggtt cgccaccttc aacgagacct tcggcgacta cagcgagaag
     2461 gcccacgagc tgctgaacac caagctggag cagtgggccg aggaggccgt ggcccgcggc
     2521 ggcttccaca acaccaccgc cctgctgatc cagtacgaga actaccgcga ctggatcgtg
     2581 aaccacaacg tggcctacag cgagctgttc ctggacaccg ccggcgtggc cagcttcgac
     2641 gtgtgggacc tgctgccctt cacccgcggc tacgtgcaca tcctggacaa ggacccctac
     2701 ctgcaccact tcgcctacga cccccagtac ttcctgaacg agctggacct gctgggccag
     2761 gccgccgcca cccagctggc ccgcaacatc agcaacagcg gcgccatgca gacctacttc
     2821 gccggcgaga ccatccccgg cgacaacctg gcctacgacg ccgacctgag cgcctggacc
     2881 gagtacatcc cctaccactt ccgccccaac taccacggcg tgggcacctg cagcatgatg
     2941 cccaaggaga tgggcggcgt ggtggacaac gccgcccgcg tgtacggcgt gcagggcctg
     3001 cgcgtgatcg acggcagcat cccccccacc cagatgagca gccacgtgat gaccgtgttc
     3061 tacgccatgg ccctgaagat cagcgacgcc atcctggagg actacgccag catgcagtaa
     3121 ggatccaccg gatctagata actgatcata atcagccata ccacatttgt agaggtttta
     3181 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     3241 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     3301 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     3361 aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     3421 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     3481 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     3541 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     3601 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     3661 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     3721 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     3781 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     3841 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3901 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3961 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     4021 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     4081 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4141 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     4201 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     4261 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     4321 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     4381 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     4441 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     4501 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
     4561 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     4621 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     4681 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     4741 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     4801 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     4861 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
     4921 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     4981 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     5041 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     5101 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     5161 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     5221 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     5281 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
     5341 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     5401 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     5461 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     5521 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     5581 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
     5641 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5701 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5761 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5821 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5881 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     5941 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6001 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6061 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6121 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6181 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6241 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6301 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6361 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     6421 ttatcccctg attctgtgga taaccgtatt accgccatgc at
//
