LOCUS       V028755                 6607 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028755
VERSION     V028755
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             635..1735
                     /label="Dusp1(NM_053769)"
                     /note="Dusp1(NM_053769)"
                     /gene="Dusp1"
     polyA_signal    1776..2000
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     promoter        2016..2539
                     /label="mCMV promoter"
     CDS             2553..3272
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    3395..3516
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3523..3978)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4005..4109
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4111..4468
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4503..5297
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5529..5576
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5905..6493
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct ccgaattcgc caccatggtg atggaggtgg gcatcctgga
      661 cgccgggggg ctgcgcgcgc tgctgcgaga gcgcgccgct cagtgcctgc ttctggattg
      721 tcgctccttc ttcgccttca acgccggcca catcgtgggc tcagtgaacg tgcgcttcag
      781 caccatcgtg cggcgccgcg ccaagggcgc catgggcctg gagcatatcg tgccgaacac
      841 cgaactgcgc ggccgcctgc tggccggagc ctatcacgcc gtagtgctgc tggacgaacg
      901 cagcgccgcc ctggacggcg ccaagcgcga cggcaccctg gccctggccg cgggcgcgct
      961 ctgccgagaa gcgcgctcca ctcaagtctt cttcctccaa ggaggatatg aagcgttttc
     1021 ggcttcctgc cctgagctgt gcagcaaaca gtccaccccc atggggctca gcctcccgct
     1081 gagtactagt gtgcctgaca gtgcagaatc cggatgcagc tcctgtagca cccctctcta
     1141 cgaccagggg ggcccagtgg agatcctgtc cttcctgtac ctgggcagtg cttaccatgc
     1201 ttcccggaaa gatatgctcg acgccttggg tatcactgct ttgatcaacg tctcggccaa
     1261 ttgtcctaac cactttgagg gtcactacca gtacaagagc atccctgtgg aggacaacca
     1321 caaggcagac attagctcct ggttcaacga ggcgattgac tttatagact ccatcaagga
     1381 tgctggagga agggtgtttg tgcactgcca ggccggcatc tccaggtcag ccaccatctg
     1441 ccttgcttac ctcatgagga ctaaccgagt gaagctggac gaggcctttg agttcgtgaa
     1501 gcagaggcgg agtattatct cccccaactt cagcttcatg ggccagctgc tgcaatttga
     1561 gtcccaagta ctggcccctc actgttctgc agaagctggg agcccggcca tggctgtcct
     1621 tgaccggggc acctctacta caacggtctt caacttccct gtctccatcc ctgttcaccc
     1681 cacgaacagt gccctgaact accttcaaag ccccatcaca acctctccga gctgctgagg
     1741 atccgggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     1801 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     1861 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     1921 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     1981 ggatgcggtg ggctctatgg tctagagata tactgagtca ttagggactt tccaatgggt
     2041 tttgcccagt acataaggtc aataggggtg aatcaacagg aaagtcccat tggagccaag
     2101 tacactgagt caatagggac tttccattgg gttttgccca gtacaaaagg tcaatagggg
     2161 gtgagtcaat gggtttttcc cattattggc acgtacataa ggtcaatagg ggtgagtcat
     2221 tgggtttttc cagccaattt aattaaaacg ccatgtactt tcccaccatt gacgtcaatg
     2281 ggctattgaa actaatgcaa cgtgaccttt aaacggtact ttcccatagc tgattaatgg
     2341 gaaagtaccg ttctcgagcc aatacacgtc aatgggaagt gaaagggcag ccaaaacgta
     2401 acaccgcccc ggttttcccc tggaaattcc atattggcac gcattctatt ggctgagctg
     2461 cgttctacgt gggtataaga ggcgcgacca gcgtcggtac cgtcgcagtc ttcggtctga
     2521 ccaccgtaga acgcagatca ccggtcgcca ccatggtgag caagggcgag gagctgttca
     2581 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     2641 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     2701 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     2761 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     2821 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     2881 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     2941 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     3001 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     3061 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     3121 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     3181 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     3241 tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga tcataatcag
     3301 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     3361 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     3421 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     3481 tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata
     3541 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     3601 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     3661 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     3721 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     3781 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     3841 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     3901 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     3961 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     4021 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4081 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt
     4141 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4201 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     4261 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     4321 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4381 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     4441 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc
     4501 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     4561 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     4621 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     4681 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     4741 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     4801 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     4861 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     4921 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     4981 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     5041 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     5101 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     5161 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     5221 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     5281 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     5341 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     5401 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     5461 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
     5521 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa
     5581 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
     5641 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
     5701 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat
     5761 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5821 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5881 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5941 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6001 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     6061 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6121 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6181 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6241 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6301 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6361 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6421 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6481 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6541 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     6601 catgcat
//
