LOCUS       pCMV-Clgn(mouse)-HA     5606 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035645
VERSION     V035645
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        27..330
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        331..534
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          672..768
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             832..858
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             892..2724
                     /label="Clgn(NM_009904)"
                     /note="Clgn(NM_009904)"
                     /gene="Clgn"
     polyA_signal    2745..2879
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(2990..3006)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    3014..3030
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(3038..3068)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    3083..3104
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(3392..3980)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(4151..5011)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(5012..5116)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     5590..5606
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat gtacccatac
      841 gatgttccag attacgctct tatggccatg gaggcccgaa ttcggtcgac tatgcgtttc
      901 cagggtgttg gactatgttt gggtctcctg ttcatcacgg tcaatgcgga ctttatggat
      961 gatggtgttg aggtggaaga cttcagtgaa aattcagacg agagtaatat taaagatgaa
     1021 ccttcctcgg ggacttttaa gtataagaca cctcaaccta taggagaagt atattttaca
     1081 gaaacatttg atagtggaaa tctggctggg tgggtcttat caaaagcaaa gaaggatgat
     1141 atggattcag aaattgctat atatgatgga agatgggaaa tagaagaact gaaggaaaac
     1201 caggtgcctg gcgacagagg gctggttctg aagtctaaag ccaagcacca cgcaatagcc
     1261 gctgtcctcg aaaaaccctt tatttttgcc gataaacctt tgatagttca atatgaagta
     1321 aattttcaag atggtattga ttgtggaggt gcatacatta aactgctagc agacactggt
     1381 gatttgattc tggaaaactt ttatgataaa acatcctata ccatcatgtt tggaccagat
     1441 aaatgtggag aggattataa acttcattta atcttccgac acaaacatcc gaaaactgga
     1501 gtttttgaag aaaagcatgc caagcctcca gatgtcgatc tgaaggagtt ctttacagac
     1561 aggaagactc acctctatac ccttgtgatg aatccggatg atacatttga agtattaatt
     1621 gatcagaaag ttgtgaacca agggaccttg ctagatgatg tggtcccgcc tatcaaccca
     1681 cccagagaaa ttgacgaccc cagtgataaa aagccagagg agtgggatga cagagccaag
     1741 atccccgacc ctaccgccgt caggccagag gactgggatg aaaatgaacc tgcccaaata
     1801 gaagactcaa gtgctgttaa acctgatggc tggcttgatg atgaaccaaa gtttatcccc
     1861 aatcctaaag ctgagaaacc tgaggactgg agtgacgaca tggatggaga atgggaggca
     1921 ccacatattc ctaatccagc ctgtcagatt ggatgtggag aatggaaacc tcccatgatc
     1981 gataacccaa aatacaaagg aatctggaga ccaccaatga tcaataatcc taactaccag
     2041 ggactctgga gtcctcagaa gattcccaac ccagattact ttgaggatga ccaccccttc
     2101 ctcctgactt ctttcagtgc gctgggctta gagctttggt ccatgactcc tgacatctac
     2161 tttgataatt ttatcatctg ttcagaaaag gaggtggccg atcagtgggc tacagatggc
     2221 tgggaactga agataatggt ggcaaatgct aatgagcctg gtgtactccg acagctggtg
     2281 atagccgcag aggagcgccc ctggctctgg ctcatgtacc tggtgatggc cggcttgccc
     2341 gttgcattag ttgcttcctt ttgttggcca aggaaagtaa agaaaaaata tgaagacacg
     2401 ggacctaaaa aaactgaatt atgcaaactg caaagcaagg ccgctctgga acaagaggca
     2461 gaggaggaga aggccccaga gaagccagaa gatgtgcaag aagaaaagaa gccaggtgaa
     2521 gctgaggtcg tcaccgtgga aaaggaagta attggtgaac cggaagagaa gagtaaggaa
     2581 gacagagaaa ctctagaagg tcaagaggaa gtgagcaagc tgagtaaatc tggatcagag
     2641 gatgagatga aggacgctga tgagagccca ggatctggag atgccccact gaagtcattg
     2701 cggaagagga gggtacggaa ggattgaggt accgcggccg cggggatcca gacatgataa
     2761 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     2821 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     2881 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     2941 cggatcctct agagtcgatc tgcaggcatg ctagcttggc gtaatcatgg tcatagctgt
     3001 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     3061 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     3121 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     3181 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     3241 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     3301 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     3361 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     3421 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     3481 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     3541 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     3601 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     3661 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     3721 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     3781 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     3841 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     3901 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     3961 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     4021 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     4081 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     4141 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     4201 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     4261 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     4321 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     4381 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     4441 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     4501 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     4561 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     4621 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     4681 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     4741 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     4801 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     4861 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     4921 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     4981 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     5041 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     5101 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     5161 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     5221 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     5281 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     5341 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     5401 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
     5461 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     5521 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     5581 cacgacgttg taaaacgacg gccagt
//
