LOCUS       40924_11591        4730 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        620..638
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             675..1115
                     /codon_start=1
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     promoter        complement(1232..1250)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    1524..1645
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1652..2107)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2134..2236
                     /label=AmpR promoter
     promoter        2240..2597
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2632..3423
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3658..3705
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4034..4622
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctgatct
      661 aagcctcctg aaagatgaag ctactgtctt ctatcgaaca agcatgcgat atttgccgac
      721 ttaaaaagct caagtgctcc aaagaaaaac cgaagtgcgc caagtgtctg aagaacaact
      781 gggagtgtcg ctactctccc aaaaccaaaa ggtctccgct gactagggca catctgacag
      841 aagtggaatc aaggctagaa agactggaac agctatttct actgattttt cctcgagaag
      901 accttgacat gattttgaaa atggattctt tacaggatat aaaagcattg ttaacaggat
      961 tatttgtaca agataatgtg aataaagatg ccgtcacaga tagattggct tcagtggaga
     1021 ctgatatgcc tctaacattg agacagcata gaataagtgc gacatcatca tcggaagaga
     1081 gtagtaacaa aggtcaaaga cagttgactg tatcgccgga attgggatcc gctagcccgg
     1141 gcgaattcgg aagcttgcgg ccgcgtcgac ctctagactg cagctcgacc tcgagggggg
     1201 gcccggtacc aggtaagtgt acccaattcg ccctatagtg agtcgtatta caattcactc
     1261 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggagatccaa tttttaagtg
     1321 tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcac agtcccaagg
     1381 ctcatttcag gcccctcagt cctcacagtc tgttcatgat cataatcagc cataccacat
     1441 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     1501 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     1561 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     1621 tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa
     1681 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     1741 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     1801 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     1861 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     1921 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     1981 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     2041 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     2101 ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     2161 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     2221 tattgaaaaa ggaagaatcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag
     2281 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     2341 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     2401 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     2461 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     2521 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     2581 gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa
     2641 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     2701 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     2761 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
     2821 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     2881 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     2941 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     3001 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     3061 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agaacatcag
     3121 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
     3181 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     3241 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     3301 gctacccgtg atattgctga agaacttggc ggcgaatggg ctgaccgctt cctcgtgctt
     3361 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     3421 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     3481 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     3541 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta
     3601 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
     3661 caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc
     3721 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc
     3781 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag
     3841 ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg
     3901 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     3961 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     4021 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4081 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4141 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     4201 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4261 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4321 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4381 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4441 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4501 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4561 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4621 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4681 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
//
