LOCUS       pCMV-3Tag-9.        5255 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian expression vector with a hygromycin resistance marker for 
            tagging proteins with a C-terminal 3xMyc epitope.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-3Tag-9
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Agilent Technologies
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     The cloned gene must include a Kozak sequence and start codon.
FEATURES             Location/Qualifiers
     source          1..5255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        620..638
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(725..741)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             744..833
                     /label=3xMyc
                     /note="3 tandem Myc epitope tags"
     misc_feature    850..860
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     promoter        complement(888..906)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    941..1148
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1155..1610)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1637..1741
                     /label=AmpR promoter
     CDS             1742..2599
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        2693..2838
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             2881..3915
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    3960..4008
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4559..5147
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
      661 gcggtggcgg ccgctctagc ccgggcggat cccccgggct gcaggaattc gatatcaagc
      721 ttatcgatac cgtcgacctc gaggagcaga aactcatctc tgaagaagat ctggaacaaa
      781 agttgatttc agaagaagat ctggaacaga agctcatctc tgaggaagat ctgtaagggc
      841 ccggtacctt aattaattaa ggtaccaggt aagtgtaccc aattcgccct atagtgagtc
      901 gtattacaat tcactcgatc ggctcgctga tcagcctcga ctgtgccttc tagttgccag
      961 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     1021 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     1081 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     1141 gctggggaac gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     1201 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     1261 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     1321 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     1381 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     1441 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     1501 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     1561 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac
     1621 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     1681 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     1741 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     1801 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     1861 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     1921 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     1981 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     2041 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     2101 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     2161 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     2221 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     2281 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     2341 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     2401 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     2461 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     2521 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     2581 ctcactgatt aagcattggc ctcaggatta gtgcttcatc cccgtggccc gttgctcgcg
     2641 tttgctggcg gtgtccccgg aagaaatata tttgcatgtc tttagttcta tgatgacaca
     2701 aaccccgccc agcgtcttgt cattggcgaa ttcgaacacg cagatgcagt cggggcggcg
     2761 cggtccgagg tccacttcgc atattaaggt gacgcgtgtg gcctcgaaca ccgagcgacc
     2821 ctgcagcgac ccgcttaaca gcgtcaacag cgtgccgcag atcccggggg gcaatgagat
     2881 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     2941 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     3001 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     3061 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     3121 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     3181 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
     3241 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     3301 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     3361 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     3421 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     3481 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     3541 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     3601 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     3661 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     3721 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     3781 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     3841 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc ggatcgggag
     3901 atgggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac
     3961 ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcat aaacgcgggg
     4021 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
     4081 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
     4141 cagccaacgt cggggcggca ggccctgcca tagccactgg ccccgtgggt tagggacggg
     4201 gtcccccatg gggaatggtt tatggttcgt gggggttatt attttgggcg ttgcgtgggg
     4261 tcaggtccac gactggactg agcagacaga cccatggttt ttggatggcc tgggcatgga
     4321 ccgcatgtac tggcgcgaca cgaacaccgg gcgtctgtgg ctgccaaaca cccccgaccc
     4381 ccaaaaacca ccgcgcggat ttctggcgcc gccggacgaa ctaaacctga ctacgacatc
     4441 tctgcccctt cttcgctggt acgaggagcg ctttgcctca ggtcatgacc aaaatccctt
     4501 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4561 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     4621 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     4681 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     4741 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     4801 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     4861 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     4921 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     4981 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     5041 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     5101 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     5161 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     5221 tatcccctga ttctgtggat aaccgtatta ccgcc
//
