LOCUS       pCMV-3Tag-8.        5236 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian expression vector with a hygromycin resistance marker for 
            tagging proteins with a C-terminal 3xFLAG epitope.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-3Tag-8
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Agilent Technologies
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     The cloned gene must include a Kozak sequence and start codon.
FEATURES             Location/Qualifiers
     source          1..5236
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        620..638
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(725..741)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             744..815
                     /label=3xFLAG
                     /note="three tandem FLAG(R) epitope tags"
     misc_feature    831..841
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     promoter        complement(869..887)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    922..1129
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1136..1591)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1618..1722
                     /label=AmpR promoter
     CDS             1723..2580
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        2674..2819
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             2862..3896
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    3941..3989
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4540..5128
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
      661 gcggtggcgg ccgctctagc ccgggcggat cccccgggct gcaggaattc gatatcaagc
      721 ttatcgatac cgtcgacctc gaggattaca aggatgacga cgataaggac tataaggacg
      781 atgatgacaa ggactacaaa gatgatgacg ataaataggg cccggtacct taattaatta
      841 aggtaccagg taagtgtacc caattcgccc tatagtgagt cgtattacaa ttcactcgat
      901 cggctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
      961 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     1021 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     1081 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggaa cgcgtaaatt
     1141 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     1201 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     1261 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     1321 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     1381 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     1441 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     1501 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     1561 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
     1621 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1681 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1741 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1801 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     1861 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     1921 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     1981 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     2041 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     2101 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     2161 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2221 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2281 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     2341 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     2401 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     2461 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2521 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2581 cctcaggatt agtgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg
     2641 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg
     2701 tcattggcga attcgaacac gcagatgcag tcggggcggc gcggtccgag gtccacttcg
     2761 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaac
     2821 agcgtcaaca gcgtgccgca gatcccgggg ggcaatgaga tatgaaaaag cctgaactca
     2881 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
     2941 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
     3001 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
     3061 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc
     3121 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
     3181 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
     3241 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
     3301 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
     3361 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
     3421 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
     3481 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
     3541 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
     3601 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
     3661 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
     3721 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
     3781 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
     3841 atagtggaaa ccgacgcccc agcactcgtc cggatcggga gatgggggag gctaactgaa
     3901 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
     3961 aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
     4021 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
     4081 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
     4141 aggccctgcc atagccactg gccccgtggg ttagggacgg ggtcccccat ggggaatggt
     4201 ttatggttcg tgggggttat tattttgggc gttgcgtggg gtcaggtcca cgactggact
     4261 gagcagacag acccatggtt tttggatggc ctgggcatgg accgcatgta ctggcgcgac
     4321 acgaacaccg ggcgtctgtg gctgccaaac acccccgacc cccaaaaacc accgcgcgga
     4381 tttctggcgc cgccggacga actaaacctg actacgacat ctctgcccct tcttcgctgg
     4441 tacgaggagc gctttgcctc aggtcatgac caaaatccct taacgtgagt tttcgttcca
     4501 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4561 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4621 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4681 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4741 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4801 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4861 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4921 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4981 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5041 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5101 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5161 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     5221 taaccgtatt accgcc
//
