LOCUS       pCMV-3Tag-4B.        4234 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian expression vector for tagging proteins with a C-terminal 
            3xMyc epitope. For other reading frames, use pCMV-3Tag-4A or 
            pCMV-3Tag-4C.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-3Tag-4B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Agilent Technologies
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     The cloned gene must include a Kozak sequence and start codon.
FEATURES             Location/Qualifiers
     source          1..4234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        620..638
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    651..744
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             745..834
                     /label=3xMyc
                     /note="3 tandem Myc epitope tags"
     misc_feature    851..861
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     promoter        complement(889..907)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    942..1149
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1156..1611)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1638..1740
                     /label=AmpR promoter
     promoter        1744..2101
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2136..2927
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3162..3209
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3538..4126
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
      661 gcggtggcgg ccgctctagc ccgggcggat cccccgggct gcaggaattc gatatcaagc
      721 ttatcgatac cgtcgacact cgaggagcag aaactcatct ctgaagaaga tctggaacaa
      781 aagttgattt cagaagaaga tctggaacag aagctcatct ctgaggaaga tctgtaaggg
      841 cccggtacct taattaatta aggtaccagg taagtgtacc caattcgccc tatagtgagt
      901 cgtattacaa ttcactcgat cggctcgctg atcagcctcg actgtgcctt ctagttgcca
      961 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     1021 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     1081 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     1141 tgctggggaa cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     1201 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     1261 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     1321 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     1381 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     1441 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     1501 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     1561 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca
     1621 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     1681 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     1741 atcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     1801 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     1861 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     1921 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     1981 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     2041 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     2101 agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     2161 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     2221 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     2281 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
     2341 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     2401 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     2461 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     2521 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     2581 aagccggtct tgtcgatcag gatgatctgg acgaagaaca tcaggggctc gcgccagccg
     2641 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
     2701 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     2761 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     2821 ctgaagaact tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     2881 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     2941 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     3001 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     3061 cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga
     3121 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
     3181 taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
     3241 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
     3301 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
     3361 aggccctgcc atagcctcag gttactcata tatactttag attgatttaa aacttcattt
     3421 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     3481 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     3541 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     3601 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     3661 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     3721 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     3781 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     3841 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3901 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     3961 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4021 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4081 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4141 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     4201 atcccctgat tctgtggata accgtattac cgcc
//
