LOCUS       pCMV-3Tag-3B.        4215 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian expression vector for tagging proteins with a C-terminal 
            3xFLAG epitope. For other reading frames, use pCMV-3Tag-3A or 
            pCMV-3Tag-3C.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-3Tag-3B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Agilent Technologies
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     The cloned gene must include a Kozak sequence and start codon.
FEATURES             Location/Qualifiers
     source          1..4215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        620..638
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    651..744
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             745..816
                     /label=3xFLAG
                     /note="three tandem FLAG(R) epitope tags"
     misc_feature    832..842
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     promoter        complement(870..888)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    923..1130
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1137..1592)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1619..1721
                     /label=AmpR promoter
     promoter        1725..2082
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2117..2908
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3143..3190
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3519..4107
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
      661 gcggtggcgg ccgctctagc ccgggcggat cccccgggct gcaggaattc gatatcaagc
      721 ttatcgatac cgtcgacact cgaggattac aaggatgacg acgataagga ctataaggac
      781 gatgatgaca aggactacaa agatgatgac gataaatagg gcccggtacc ttaattaatt
      841 aaggtaccag gtaagtgtac ccaattcgcc ctatagtgag tcgtattaca attcactcga
      901 tcggctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
      961 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     1021 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     1081 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga acgcgtaaat
     1141 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     1201 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     1261 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     1321 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     1381 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     1441 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     1501 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     1561 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     1621 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     1681 ataaccctga taaatgcttc aataatattg aaaaaggaag aatcctgagg cggaaagaac
     1741 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     1801 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     1861 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     1921 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     1981 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     2041 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     2101 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     2161 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     2221 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     2281 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     2341 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     2401 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     2461 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     2521 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     2581 ggatgatctg gacgaagaac atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     2641 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     2701 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     2761 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagaac ttggcggcga
     2821 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     2881 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     2941 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     3001 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     3061 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
     3121 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
     3181 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     3241 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     3301 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
     3361 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     3421 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     3481 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     3541 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     3601 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     3661 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     3721 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     3781 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     3841 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     3901 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     3961 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4021 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4081 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     4141 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     4201 aaccgtatta ccgcc
//
