LOCUS       V014155                 6471 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014155
VERSION     V014155
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6471)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             627..2375
                     /gene="gox"
                     /label="Glucose oxidase"
                     /note="Glucose oxidase from Aspergillus niger. Accession#:
                     P13006"
     CDS             2409..3095
                     /label="AmCyan1"
                     /note="Anemonia majano cyan fluorescent protein"
     polyA_signal    3259..3380
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3387..3842)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3869..3973
                     /label="AmpR promoter"
     promoter        3975..4332
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4367..5158
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5393..5440
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5769..6357
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagcc accatgagca acggcatcga ggccagcctg ctgaccgacc
      661 ccaaggacgt gagcggccgc accgtggact acatcatcgc cggcggcggc ctgaccggcc
      721 tgaccaccgc cgcccgcctg accgagaacc ccaacatcag cgtgctggtg atcgagagcg
      781 gcagctacga gagcgaccgc ggccccatca tcgaggacct gaacgcctac ggcgacatct
      841 tcggcagcag cgtggaccac gcctacgaga ccgtggagct ggccaccaac aaccagaccg
      901 ccctgatccg cagcggcaac ggcctgggcg gcagcaccct ggtgaacggc ggcacctgga
      961 cccgccccca caaggcccag gtggacagct gggagaccgt gttcggcaac gagggctgga
     1021 actgggacaa cgtggccgcc tacagcctgc aggccgagcg cgcccgcgcc cccaacgcca
     1081 agcagatcgc cgccggccac tacttcaacg ccagctgcca cggcgtgaac ggcaccgtgc
     1141 acgccggccc ccgcgacacc ggcgacgact acagccccat cgtgaaggcc ctgatgagcg
     1201 ccgtggagga ccgcggcgtg cccaccaaga aggacttcgg ctgcggcgac ccccacggcg
     1261 tgagcatgtt ccccaacacc ctgcacgagg accaggtgcg cagcgacgcc gcccgcgagt
     1321 ggctgctgcc caactaccag cgccccaacc tgcaggtgct gaccggccag tacgtgggca
     1381 aggtgctgct gagccagaac ggcaccaccc cccgcgccgt gggcgtggag ttcggcaccc
     1441 acaagggcaa cacccacaac gtgtacgcca agcacgaggt gctgctggcc gccggcagcg
     1501 ccgtgagccc caccatcctg gagtacagcg gcatcggcat gaagagcatc ctggagcccc
     1561 tgggcatcga caccgtggtg gacctgcccg tgggcctgaa cctgcaggac cagaccaccg
     1621 ccaccgtgcg cagccgcatc accagcgccg gcgccggcca gggccaggcc gcctggttcg
     1681 ccaccttcaa cgagaccttc ggcgactaca gcgagaaggc ccacgagctg ctgaacacca
     1741 agctggagca gtgggccgag gaggccgtgg cccgcggcgg cttccacaac accaccgccc
     1801 tgctgatcca gtacgagaac taccgcgact ggatcgtgaa ccacaacgtg gcctacagcg
     1861 agctgttcct ggacaccgcc ggcgtggcca gcttcgacgt gtgggacctg ctgcccttca
     1921 cccgcggcta cgtgcacatc ctggacaagg acccctacct gcaccacttc gcctacgacc
     1981 cccagtactt cctgaacgag ctggacctgc tgggccaggc cgccgccacc cagctggccc
     2041 gcaacatcag caacagcggc gccatgcaga cctacttcgc cggcgagacc atccccggcg
     2101 acaacctggc ctacgacgcc gacctgagcg cctggaccga gtacatcccc taccacttcc
     2161 gccccaacta ccacggcgtg ggcacctgca gcatgatgcc caaggagatg ggcggcgtgg
     2221 tggacaacgc cgcccgcgtg tacggcgtgc agggcctgcg cgtgatcgac ggcagcatcc
     2281 cccccaccca gatgagcagc cacgtgatga ccgtgttcta cgccatggcc ctgaagatca
     2341 gcgacgccat cctggaggac tacgccagca tgcagggagg agggggaagc gatccaccgg
     2401 tcgccaccat ggccctgtcc aacaagttca tcggcgacga catgaagatg acctaccaca
     2461 tggacggctg cgtgaacggc cactacttca ccgtgaaggg cgagggcagc ggcaagccct
     2521 acgagggcac ccagacctcc accttcaagg tgaccatggc caacggcggc cccctggcct
     2581 tctccttcga catcctgtcc accgtgttca tgtacggcaa ccgctgcttc accgcctacc
     2641 ccaccagcat gcccgactac ttcaagcagg ccttccccga cggcatgtcc tacgagagaa
     2701 ccttcaccta cgaggacggc ggcgtggcca ccgccagctg ggagatcagc ctgaagggca
     2761 actgcttcga gcacaagtcc accttccacg gcgtgaactt ccccgccgac ggccccgtga
     2821 tggccaagaa gaccaccggc tgggacccct ccttcgagaa gatgaccgtg tgcgacggca
     2881 tcttgaaggg cgacgtgacc gccttcctga tgctgcaggg cggcggcaac tacagatgcc
     2941 agttccacac ctcctacaag accaagaagc ccgtgaccat gccccccaac cacgtggtgg
     3001 agcaccgcat cgccagaacc gacctggaca agggcggcaa cagcgtgcag ctgaccgagc
     3061 acgccgtggc ccacatcacc tccgtggtgc ccttctgaga attctgcagt cgacggtacc
     3121 gcgggcccgg gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta
     3181 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     3241 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     3301 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     3361 aaactcatca atgtatctta acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc
     3421 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     3481 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     3541 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     3601 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     3661 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     3721 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     3781 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     3841 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3901 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3961 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     4021 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4081 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     4141 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     4201 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     4261 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     4321 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     4381 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     4441 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     4501 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     4561 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     4621 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     4681 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     4741 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     4801 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     4861 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
     4921 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     4981 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     5041 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     5101 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     5161 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     5221 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     5281 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg
     5341 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     5401 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
     5461 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
     5521 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
     5581 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta
     5641 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5701 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     5761 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5821 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5881 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     5941 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6001 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6061 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6121 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6181 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6241 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6301 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6361 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     6421 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca t
//
