LOCUS       62056_6575        9015 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9015)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9015
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        50..429
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        430..633
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             744..770
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     CDS             774..800
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     CDS             804..830
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     CDS             2692..2703
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     misc_feature    3791..4379
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    4445..4493
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4695..5123
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5137..5466
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             5533..6324
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6503..6636
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6673..6689)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6697..6713)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6721..6751)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6766..6787)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7075..7663)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7837..8694)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8695..8799)
                     /label=AmpR promoter
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      721 actctagagc tagcgccacc atgtacccat acgacgtacc tgattatgca acttatcctt
      781 atgatgtacc tgattatgct agctatcctt atgatgtccc agactacgca aagcttggta
      841 ccatggctac cgagagtact ccctcagaga tcatagaaag agaaagaaaa aagttgcttg
      901 aaatccttca acatgatcct gattctatct tagacacgtt aacttctcgg aggctgattt
      961 ctgaggaaga gtatgagact ctggagaatg ttacagatct cctgaagaaa agtcggaagc
     1021 tgttaatttt ggtacagaaa aagggagagg cgacctgtca gcattttctc aagtgtttat
     1081 ttagtacttt tccacagtca gctgccattt gcggcttaag gcatgaagtt ttaaaacatg
     1141 agaatacagt acctcctcaa tctatggggg caagcagtaa ttcagaagat gctttttctc
     1201 ctggaataaa acagcctgaa gcccctgaga tcacagtgtt cttcagtgag aaggaacact
     1261 tggatttgga aacctctgag tttttcaggg acaagaaaac tagttatagg gaaacagctt
     1321 tgtctgccag gaagaatgag aaggaatatg acacaccaga agtcacatta tcatattcag
     1381 ttgagaaagt tggatgtgaa gttccagcaa ctattacata tataaaagat ggacagagat
     1441 atgaggagct agatgattct ttatacttag gaaaagagga atatctagga tctgttgaca
     1501 cccctgaaga tgcagaagcc actgtggaag aggaggttta tgatgaccca gagcacgttg
     1561 gatatgatgg tgaagaggac ttcgagaatt cagaaaccac agagttctct ggtgaagaac
     1621 caagttatga gggatcagaa accagccttt cattggagga ggaacaggag aaaagtatag
     1681 aagaaagaaa aaaggtgttt aaagatgtcc tgttatgttt gaacatggat agaagcagaa
     1741 aggttctgcc agattttgtt aaacaattct ccttagatcg aggatgtaag tggacccctg
     1801 agagtccagg agacttagcc tggaatttcc tgatgaaagt tcaagcacga gatgtgacgg
     1861 ctagggattc aatcctcagt cacaaggttc tggatgaaga tagcaaggag gatttgctgg
     1921 ctggagtgga gaatttggaa attcgagaca tacaaaccat taatcccctt gacgtgcttt
     1981 gtgccaccat gctgtgttca gatagctctt tgcaacgcca agtcatgtca aacatgtatc
     2041 agtgccagtt tgctcttccc ctgctactgc cagatgcaga aaacaacaaa agcatcttaa
     2101 tgctgggggc catgaaagac attgtgaaga agcagtcaac acagttttca ggggggccta
     2161 cagaggatac agaaaagttt ctgactctca tgaagatgcc tgtcatctct tttgtgcgtc
     2221 taggatactg tagcttctct aagtccagaa tcctcaacac acttctcagc cctgcccagt
     2281 tgaaattaca caaaatcttt cttcatcaag atttgcctct tttggtgctt ccccggcaaa
     2341 tctctgatgg cctggttgag ataacatggt gttttcctga tagcgatgat agaaaggaaa
     2401 accccttttt ccaaaagcct gttgctctgg ctaatctccg tggaaatcta gaaagctttt
     2461 ggactcagtt tggttttttg atggaagttt cttcagctgt gttttttttc actgactgtt
     2521 taggtgagaa ggaatgggac ttgctaatgt ttttaggaga ggctgccatt gaaagatgct
     2581 actttgttct cagttcccaa gccagggaga gtgaagaggc tcaaattttt cagaggatac
     2641 tgaacttgaa gccagcacag ctactgtttt gggagagggg agatgctggg gatagaagga
     2701 agaacatgga gggccttcaa gctgccctcc aggaagtgat gttctcttct tgcctcagat
     2761 gtgtgtctgt ggaggatatg gccgccctgg ccagggagct ggggattcag gtagatgaag
     2821 actttgaaaa cactcagaga attcaagttt cctctggaga aaacatggct gggacagctg
     2881 aaggtgaggg tcagcaaaga cacagtcagc taaaaagctc atctaaaagc caggctctaa
     2941 tgccaattca agagcctggg actcaatgtg agctcagcca gaatcttcag aatctctatg
     3001 gtaccccagt attcaggcct gttctagaga actcctggct ctttccaacc agaattggag
     3061 gtaactttaa ccatgtttcc ttgaaagcct cctgggttat gggccgcccc tttgggtcag
     3121 agcagaggcc taagtggttc catcctttgc cttttcagaa tgcaggggcc cagggccgag
     3181 gtaaaagttt tggtattcaa tccttccatc cccagatatt ttattcaggt gaaagattca
     3241 tgaaattttc cagagttgct cggggatgtc actcgaatgg aacatttggg agactgccaa
     3301 gacccatttg tcagcatgta caggcctgcc ctgagagacc acaaatgatg ggaactcttg
     3361 aaaggtctag ggcagtagcc tccaagatag gtcactccta ttccctggat tcacagccag
     3421 caagagcagt agggaagcca tggcctcagc aagcttgcac cagggtaaca gagttaactg
     3481 aagcaactgg aaaactgata agaacatccc atattggaaa gcctcaccct cagtcctttc
     3541 aaccagcagc agccacacaa aaactaagac ctgcttctca gcaaggagtc cagatgaaga
     3601 cacaaggtgg ggcttcaaat ccagctctcc aaatagggtc ccatcccatg tgcaagagct
     3661 ctcagttcaa atccgatcag tccaacccat aagaattctg cagatatcca gcacagtggc
     3721 ggccgctcga gtctagaggc cccttaaggg ttcgatccct accggttagt aatgagtttg
     3781 atatctcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     3841 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     3901 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     3961 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     4021 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     4081 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     4141 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     4201 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     4261 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     4321 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     4381 tggaaacggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
     4441 atgacggcaa taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg
     4501 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
     4561 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
     4621 gctcgcagcc aacgtcgggg cggcaggccc tgccatagca gatctgcgca gctggggctc
     4681 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     4741 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     4801 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt
     4861 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     4921 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     4981 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     5041 ttcttttgat ttataaggga ttttggggat ttcggcctat tggttaaaaa atgagctgat
     5101 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     5161 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     5221 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     5281 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     5341 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     5401 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     5461 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga
     5521 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     5581 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     5641 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     5701 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     5761 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     5821 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     5881 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     5941 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     6001 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     6061 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     6121 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     6181 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     6241 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     6301 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgcgaaa tgaccgacca
     6361 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     6421 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     6481 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     6541 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     6601 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     6661 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     6721 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     6781 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     6841 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     6901 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     6961 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7021 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7081 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7141 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7201 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7261 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7321 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7381 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     7441 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7501 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     7561 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7621 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7681 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     7741 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     7801 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     7861 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     7921 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     7981 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     8041 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     8101 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     8161 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     8221 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     8281 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     8341 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     8401 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     8461 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     8521 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     8581 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     8641 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     8701 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     8761 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     8821 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc
     8881 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
     8941 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
     9001 aagaatctgc ttagg
//
