LOCUS       40924_11451        8964 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCMH500, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Strive T, Hardy CM, French N, Wright JD, Nagaraja N, Reubel GH.
  TITLE     Development of canine herpesvirus based antifertility vaccines for 
            foxes using bacterial artificial chromosomes
  JOURNAL   Vaccine 24 (7), 980-988 (2006)
  PUBMED    16198458
REFERENCE   2  (bases 1 to 8964)
  AUTHORS   Hardy CM.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2004) Sustainable Ecosystems, CSIRO, GPO Box 284, 
            Canberra, ACT 2601, Australia
REFERENCE   3  (bases 1 to 8964)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8964)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Vaccine"; 
            date: "2006"; volume: "24"; issue: "7"; pages: "980-988"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-SEP-2004) Sustainable Ecosystems, CSIRO, GPO Box 284, Canberra, 
            ACT 2601, Australia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8964
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1..6089
                     /isolate="greyhound"
                     /mol_type="other DNA"
                     /country="Australia"
                     /db_xref="taxon:170325"
                     /organism="Canid herpesvirus 1"
     misc_feature    1..1095
                     /label=similar to UL25
                     /note="similar to UL25"
     CDS             complement(1153..1935)
                     /codon_start=1
                     /product="UL24"
                     /label=UL24
                     /protein_id="AAX18922.1"
                     /translation="MKRNQRQTIRTRLCAGIRCHNRFYSVLTRDVKLASQNGIYSDRLS
                     LLFGDLIPADILKRAGSVNLAFEVNLGQRRPDCVCTINLEHESMGLCILIELKTCRFSK
                     NMNTASKTTQQRGGLKQLNDSVSLITKILPPGDGKVVLIPLLVFIAQRGMNILKVTSLP
                     HKQLSSNIKTLAANLTKLAEYIPKPAKPVKGSNNRNKSLKQKVNCLKCNFVNCICIKTS
                     NVEFINLTNTEKSTTCSNFESSTLLNPVKLISSLFNKD"
     misc_feature    1947..2460
                     /label=similar to UL23 thymidine kinase
                     /note="similar to UL23 thymidine kinase"
     misc_feature    2268..2275
                     /note="NotI cloning site for insertion of transgenes;
                     disrupts UL23 sequence"
     CDS             2570..4960
                     /codon_start=1
                     /product="UL22"
                     /label=UL22
                     /note="glycoprotein H"
                     /protein_id="AAX18923.1"
                     /translation="MEVKYNFITFIFVIFFMLVFSNDLDNGEYKNLSLSNDFSYSSNVN
                     ITTIENLFSYNNKFVFFFIFNKDSKRENGNLFLFPNFIFKSNKTENNYNIKSQPSEKQL
                     TVFFNPYINLYLSSKYLIDFGVIPESSIQDWYFPRSIQTSTEQNPFGFILSPKRHVPNN
                     GFFNKHKNLYKDGLLNDIMYVNLNSFSIPRKTYIPIYESTWPFDISLFESTLIQNENFC
                     INITIGKEFMGMSITSSKYPPLEMITTPHDANVEMITRYKASMMLDPPGPSEGPLYKVF
                     IIGYGTSKTDSNMYKIMQTIASYPEESLDYRYHLSMANFEVCFLLTYERKFLTNKMDME
                     YFYKIFSRLSTALFSLSEMVRLSKYILNDEIIDIDFNINILTNIIVNHKMFKLSKQLIT
                     SSQVNILLESSRENIVNNLNTFENYNLIFKNDELKIKYLKAVYTYLEIPTEKSLIISRD
                     IINTLYNNSLYETIDWNVTSRKSLFLATSLLLLKTQNIVELRKIILQCTSMCTSDHAIS
                     IEWSLENILNAKNNFKKQFSITDMFSPCMTSTRYDLIEDLHIVDLISVIPNNGNFKNLF
                     SMGHKAASVVNSNILFGSNKIKMLIPELFICKIDINFKFLAILPLNNNNSYIITRKILN
                     RGLTYNVNGIDIANPIFISYINSSNCLYSSVEILPLNLINPKNSKECLYCGSVIMRYLS
                     SGVIIDLLYINDKEAETQLAAGVNSTIPSFNPLSYPVNLKTLLLFPNGTIVTINSITSH
                     EQIKFSNTFIVISIVGIIITSFVVYGIFKMLCSFSYVKYSLLK"
     misc_feature    5003..5420
                     /label=GC-rich region
                     /note="GC-rich region"
     misc_feature    5576..6089
                     /label=similar to UL21
                     /note="similar to UL21"
     promoter        complement(6107..6125)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6132..6148)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(6289..6744)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6771..6875
                     /label=AmpR promoter
     CDS             6876..7733
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7907..8495
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    8783..8804
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8819..8849
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8857..8873
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8881..8897
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8918..8936
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tctagataaa aatacagcag cggcgttatt tactggatca acatatgcag tttgctttac
       61 atttaaatct actaccaaat catttgtacc catacctaaa ctacctggaa tagcgggtaa
      121 tatttttaat ttaagaagca gttttaaaat actatgatca ttaagtgcat tacgttcata
      181 tcttccacca ttaggtgcat gaaattggtt tcttggtaat ctatcaatat taaacatata
      241 ggatactcta gtagatatag tttcccctaa ggattgtgag attgaatcca aatatagtgg
      301 tagttgttcc aaaagttcta taaagtttgt ggtaattatt ttactatttg gatttaattc
      361 tctataaaca agatgtaaac ataaaactgc acattcaaat gcggaatatg gtctatttcc
      421 gatatataga cgactacatg attgaagtga cattatagta gttagcataa aagttttaga
      481 tattctacca tctctaaaat ctaaacttcg tgtaattaaa ggtctatcca aaatcaattt
      541 atcttgtaaa gacctatacc atggaccaaa aacaacacca tttgatcccc ctgcaccacg
      601 acctatataa accatagtta gaaaatctgt agctaaattt gtatcatact ccaaaggagt
      661 gtcaattttt gtaatttgta cttctctagt tggtgtatta gtttcatctt caaaattttg
      721 tgtagattca ctttgagtat catcatcatt ttgtatatct gcatcctctg ctattttagc
      781 agattcttcc aaagatgata gcgcttttgc tacatttgac acctgttttt ctaaaggata
      841 taaacgtttt tcaatatcac cagaatctaa cattgcctgt gcacttaaat tatctaaagc
      901 agctactgta gcagttcgcc gtgcaatcat aacttcaagc cgttgttccg ctgtaattat
      961 aggcgcatta aaaaccggtt cattccaata attaattggg aacgctgggg cgatgaaatt
     1021 tctaatatcc gatggtatga ttaaattatt tgactctcgt atagcatcaa aaacatatga
     1081 aacatagtta gacattttaa ttgatgaaac tatatcataa acgcacaaca aacaaatgag
     1141 atattactag ttttaatctt tattaaataa ggatgatatc aatttaacgg ggtttaataa
     1201 tgttgaagat tcaaaatttg aacatgtagt ggatttttca gtgttagtta aatttataaa
     1261 ctcaacatta cttgttttaa tacatataca atttacaaaa ttacatttta ggcaattaac
     1321 tttttgtttt aaagatttat ttctgttgtt agaccctttt acaggctttg cgggttttgg
     1381 tatatattct gccaatttag ttaaattagc tgctaaagtt ttaatattac tacttagctg
     1441 tttatgaggt aaactagtaa ctttaagaat attcatccca cgttgagcta taaaaactaa
     1501 taaaggaatt aatactacct taccatcccc aggtggtagt atcttcgtta tcaaactgac
     1561 agaatcattt aattgtttta acccacccct ttgttgagta gttttacttg cagtgttcat
     1621 atttttagaa aatctacatg tttttaattc aattaaaata cataaaccca tagattcatg
     1681 ttctaaatta atagtacata cacaatctgg tctccgttga cctaagttaa cctcaaaagc
     1741 caaatttaca cttcccgccc ttttaaggat atcagctggt atcaagtccc caaacaaaag
     1801 agaaagacgg tcactataaa ttccattttg tgaggcgagt ttcacatctc ttgttaatac
     1861 actataaaag cgattgtggc atctaatccc agcacaaagt cttgttctaa ttgtttgtcg
     1921 ttgatttcgt ttcatcgtcc aaaaacatgg aagtaaaaat ttgccgcttt tatatagatg
     1981 gttgttatgg aacaggaaaa agtacagctg cattaaattt tattaaatta gaaaaaaata
     2041 atccagtata ttattttccg gaacctatgg cattctggcg tatcatccta gaaacagata
     2101 ttgtgcaagg tatatactca gtacaagacc ggaagctgcg tggtgaatta agcctaaatg
     2161 atgcgtcatt aattacagct caacttcaaa ctaaattttc tacgccatat attttacttc
     2221 attccaatgt atccaaattt tttggagaaa atgtaacatt tggaattgcg gccgcagctt
     2281 gttgacagtt tagaaaatct taaaattttt aaagttagct ttcagggttc acccgacgtt
     2341 tgtgtatcta atattcgcga aattataccc catatgacta taacctcaac atcgaaaagg
     2401 tttttacaaa accttattaa aacttcaaaa agtttttcta aagaaatgta ttgtgattaa
     2461 ttaaagttta ataaataaat ttgtatcaag tgtaagtttt tttttttgga tataaaaacg
     2521 agtaaaccta taaaagaaaa cttcattttt taaattttaa tcattcaaca tggaagtaaa
     2581 atataacttt attacgttta tatttgttat tttttttatg ttggtttttt cgaatgattt
     2641 ggataatggt gagtataaaa atttatcttt aagtaatgat tttagttata gtagtaatgt
     2701 taatataact actatagaaa atctatttag ttataataat aaatttgtat ttttttttat
     2761 ctttaataaa gatagtaaac gagaaaatgg aaatttattt ttatttccca actttatttt
     2821 caaatctaat aaaacagaaa ataactacaa tattaaatct caaccgtcgg aaaaacaatt
     2881 aacggttttt tttaatcctt atataaattt atatttatca agtaaatatt taattgattt
     2941 tggagttatt cctgaatctt ctattcaaga ttggtatttt cctagatcta tacaaacatc
     3001 aacggaacaa aatccatttg gttttattct atccccaaaa agacatgtac ctaataatgg
     3061 tttttttaat aaacataaaa atttatataa agatggactt ttaaatgata taatgtatgt
     3121 aaatttaaac tctttttcaa taccaagaaa aacctatata cctatatatg aatctacatg
     3181 gccttttgat atttctttat ttgaatcaac attaattcaa aatgaaaatt tttgtattaa
     3241 tataactatt ggaaaagaat ttatgggaat gtctataact tcttcaaaat atccaccatt
     3301 ggaaatgatt accactcctc atgatgctaa tgtagaaatg attacaagat ataaagcaag
     3361 tatgatgtta gatccacccg gcccatccga aggaccatta tataaagttt ttataattgg
     3421 atatggaaca tcaaaaacgg atagtaatat gtataaaata atgcaaacaa tcgcatcata
     3481 tccagaagag agtcttgatt atcgatatca tctttctatg gcaaattttg aagtttgttt
     3541 tttgttaaca tatgaacgta agtttttaac taataaaatg gatatggaat atttttataa
     3601 aattttttcg agattatcaa ctgcattatt ttctctatct gaaatggtaa gactaagtaa
     3661 atatatttta aatgatgaaa ttattgatat agattttaat attaatattt taaccaatat
     3721 tatagtaaat cataaaatgt ttaaattatc aaaacaatta ataacgtcat ctcaagtaaa
     3781 tattctattg gaatcatcaa gagaaaatat tgttaataac cttaatactt ttgaaaatta
     3841 taatttaata tttaaaaatg atgaattaaa aattaaatac ctaaaagctg tttatacata
     3901 cttggaaatt ccaactgaaa aaagtttaat tatctcaaga gatattatta atacactata
     3961 taataactct ttatatgaaa caattgattg gaatgttact tcacgtaaaa gtttattttt
     4021 ggcgacatct ttattattgt taaaaacaca aaatattgta gagttaagaa aaattatttt
     4081 acaatgtact tcgatgtgta cttctgatca cgctatctct atagaatgga gtttggaaaa
     4141 tatcttaaat gctaaaaata attttaaaaa acaatttagt attactgata tgttctcccc
     4201 ttgtatgact tcaactcgtt atgatttaat tgaagattta catattgttg atttaatttc
     4261 tgttatacca aacaatggaa attttaaaaa tttgttttct atgggacaca aggctgcatc
     4321 tgtggttaat tctaatattt tatttggatc aaataaaatt aaaatgttaa ttccagagct
     4381 ttttatatgt aagatagata taaactttaa atttttagct atattaccac ttaataacaa
     4441 taacagttac ataataacac gtaaaatttt aaaccgtggt ttaacctata atgtaaatgg
     4501 tatagatata gctaatccaa tttttatttc ttatataaat tccagtaatt gtttatattc
     4561 tagtgttgaa atattacctt tgaatttaat taatccaaaa aattcaaaag agtgtttata
     4621 ttgtggaagt gtaattatgc gttatttatc atcaggtgtt ataattgatt tactttatat
     4681 aaatgataag gaagctgaaa cacaacttgc tgctggagta aattcaacaa taccatcctt
     4741 taatccttta tcataccctg ttaatttaaa aacattactt ttatttccaa atggaacaat
     4801 agttactatc aattcaataa catctcatga acaaataaag ttttcaaata cctttattgt
     4861 tatatcgata gttggtataa taataactag ttttgtggtt tatggaatct ttaaaatgtt
     4921 atgtagtttt tcatatgtta aatattcact tttaaaataa attaattaat cataaaaatt
     4981 tgttgtatgc gggttaatta ctgggatcgg ggggttgagg acgcggatgg ttcacggcac
     5041 gccggatcga gcgtgatact gggatcgggg ggttgaggac gcggatggtt cacggcacgc
     5101 cggatcgagc gtgatactgg gatcgggggg ttgaggacgc ggatggttca cggcacgccg
     5161 gatcgagcgt gatactggga tcggggggtt gaggacgcgg atggttcacg gcacgccgga
     5221 tcgagcgtga tactgggatc ggggggttga ggacgcggat ggttcacggc acgccggatc
     5281 gagcgtgata ctgggatcgg ggggttgagg acgcggatgg ttcacggcac gccggatcga
     5341 gcgtgatact gggatcgggg ggttgaggac gcggatggtt cacggcacgc cggatcgagc
     5401 gtgatactgg gatcgggggg ttgaggacgc ggatgtttta tacccatttt atgcatattc
     5461 ataaagtggg tatgaatata tattattcgc acttaaaatc aataatatat attataagtt
     5521 attgaacttt tggtgcgaac gtaaataatt tcgctgtaca cggaaaaatt ttactttatt
     5581 ttattgacat atttatacgt tccttttgaa atcgcttaga tacaagttta tgtaaaaaat
     5641 aagcccctct catcacagaa atatttgtat caaaagcact aacatactta ttttcaagag
     5701 caatatgaaa aggaattgag ttttttgttg atcgagataa agccactcca caatcccaaa
     5761 tatctttacc agtatataaa cattccaata tttttgatat ttcaaaactg attcgatcta
     5821 ttttttccat agacataaat tgtctatttt tattttctaa ttccattaca aaagaagtta
     5881 atatttcaga atcagattct atatttgttg taaatttttt atatcctatc ataccaggta
     5941 aatttaaatg caaaatcagt atactcatat taccaagtat agaagatttt ctaattattt
     6001 catttgctga atcgagaatt atatttacat ttaatttttg tattggatgt tgatttactt
     6061 gaatatagct attagttaat ttttctagag cggccctcca attcgcccta tagtgagtcg
     6121 tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     6181 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     6241 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggaa attgtaagcg
     6301 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     6361 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     6421 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     6481 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     6541 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     6601 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     6661 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     6721 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     6781 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     6841 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     6901 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     6961 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     7021 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     7081 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     7141 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     7201 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     7261 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     7321 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     7381 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     7441 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     7501 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     7561 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     7621 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     7681 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     7741 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7801 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7861 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7921 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7981 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     8041 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     8101 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     8161 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     8221 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8281 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8341 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8401 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8461 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8521 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8581 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     8641 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     8701 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     8761 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     8821 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     8881 caggaaacag ctatgaccat gattacgcca agctcgaaat taaccctcac taaagggaac
     8941 aaaagctggg tacgatccac tagt
//
