LOCUS       40924_11446        3551 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCMH411, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Hardy CM.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2002) Wildlife, Pests and Diseases, CSIRO 
            Sustainable Ecosystems, GPO Box 284, Canberra, ACT 2601, Australia
REFERENCE   2  (bases 1 to 3551)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3551)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (14-JUN-2002) Wildlife, Pests and Diseases, CSIRO Sustainable 
            Ecosystems, GPO Box 284, Canberra, ACT 2601, Australia"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        149..351
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    437..456
                     /label=multiple cloning site
                     /note="multiple cloning site"
     polyA_signal    455..575
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      602..611
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(642..660)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(667..683)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      824..1279
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1360..1464
                     /label=AmpR promoter
     CDS             1465..2322
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2496..3084
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3372..3393
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3408..3438
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3446..3462
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3470..3486
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3504..3522
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gagctctccc atatggtcga cctgcaggcg gccgcgaatt agcttgcatg cctgcaggtc
       61 aattccctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      121 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt ggcagtacat caatgggcgt
      181 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      241 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      301 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
      361 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
      421 gaccgatcca gcctggggat cccgggtacc gaattcttgt ttattgcagc ttataatggt
      481 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
      541 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgcggccg caatcccgcg
      601 gccatggcgg ccgggagcat gcgacgtcgg gcccaattcg ccctatagtg agtcgtatta
      661 caattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
      721 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
      781 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc
      841 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
      901 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
      961 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagagcttt acggcacctc
     1021 gaccgcaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     1081 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     1141 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     1201 tcggcctatt ggttaaaaaa tgagctgatt taacaaatat ttaacgcgaa ttttaacaaa
     1261 atattaacgt ttacaatttc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     1321 ttcacaccgc atacaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     1381 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     1441 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     1501 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     1561 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     1621 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     1681 ctgctatgtc atacactatt atcccgtatt gacgccgggc aagagcaact cggtcgccgg
     1741 gcgcggtatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     1801 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     1861 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     1921 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     1981 aacgacgagc gtgacaccac gatgcctgta gcaatgccaa caacgttgcg caaactatta
     2041 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     2101 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     2161 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     2221 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     2281 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     2341 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     2401 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     2461 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2521 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2581 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2641 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2701 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2761 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2821 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     2881 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     2941 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3001 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3061 tcaggggggc ggagcctatc gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3121 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3181 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3241 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     3301 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     3361 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     3421 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     3481 tatgaccatg attacgccaa gctatttagg tgacactata gaatactcaa gctatgcatc
     3541 caacgcgttg g
//
