LOCUS       V008422                 7187 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008422
VERSION     V008422
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7187)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7187)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7187)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..54
                     /label="myr"
                     /note="N-myristoylation signal from Src kinase (Pellman et
                     al., 1985; Kaplan et al., 1988)"
     CDS             61..381
                     /label="FKBP"
                     /note="human FK506-binding protein FKBP12"
     CDS             388..708
                     /codon_start=1
                     /note="unnamed protein product; hFKBP1A"
                     /protein_id="SJL87495.1"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             715..2151
                     /gene="Casp8"
                     /label="Caspase-8"
                     /note="Caspase-8 from Mus musculus. Accession#: O89110"
     CDS             2158..2184
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     intron          2216..2788
                     /label="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     CDS             2792..2914
                     /gene="HBB"
                     /label="Hemoglobin subunit beta"
                     /note="Hemoglobin subunit beta from Colobus guereza.
                     Accession#: Q9TT33"
     polyA_signal    2985..3040
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     rep_origin      complement(3393..3528)
                     /direction=LEFT
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     rep_origin      complement(3805..4393)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4564..5436)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin"
                     /protein_id="SJL87497.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFQVEVAMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDL
                     VEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTR
                     LDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAE
                     IGASLIKHW"
     promoter        complement(5437..5541)
                     /label="AmpR promoter"
     rep_origin      complement(5873..6328)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     6473..6489
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6496..6514
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        6533..6836
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6837..7040
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
ORIGIN
        1 gaattcgcgc gtatggggag tagcaagagc aagcctaagg accccagcca gcgctctaga
       61 ggagtgcagg tggaaaccat ctccccggga gacgggcgca ccttccccaa gcgcggccag
      121 acctgcgtgg tgcactacac cgggatgctt gaagatggaa agaaatttga ttcctcccgg
      181 gacagaaaca agccctttaa gtttatgcta ggcaagcagg aggtgatccg aggctgggaa
      241 gaaggggttg cccagatgag tgtgggtcag agagccaaac tgactatatc tccagattat
      301 gcctatggtg ccactgggca cccaggcatc atcccaccac atgccactct cgtcttcgat
      361 gtggagcttc taaaactgga agtcgagggc gtgcaggtgg aaaccatctc cccaggagac
      421 gggcgcacct tccccaagcg cggccagacc tgcgtggtgc actacaccgg gatgcttgaa
      481 gatggaaaga aatttgattc ctcccgggac agaaacaagc cctttaagtt tatgctaggc
      541 aagcaggagg tgatccgagg ctgggaagaa ggggttgccc agatgagtgt gggtcagaga
      601 gccaaactga ctatatctcc agattatgcc tatggtgcca ctgggcaccc aggcatcatc
      661 ccaccacatg ccactctcgt cttcgatgtg gagcttctaa aactggaaac tagtgatttc
      721 cagagttgtc tttatgctat tgctgaagaa ctgggcagtg aagacctggc tgccctcaag
      781 ttcctgtgct tggactacat cccacacaag aagcaggaga ccatcgagga tgcccagaag
      841 ctatttctga ggcttcggga aaaggggatg ttggaggaag gcaatctgtc tttcctgaaa
      901 gagctgcttt tccacatcag tcggtgggac ctgctggtca acttcctaga ctgcaaccga
      961 gaggagatgg tgagagagct gcgggaccca gacaatgtcc agatttctcc ctacagggtc
     1021 atgctcttta agctctcaga agaagtgagc gagttggaat tgagatcttt taagttcctt
     1081 ttgaacaatg agatccccaa atgtaagctg gaagatgact tgagcctgct tgaaattttt
     1141 gtagaaatgg agaagaggac catgctggca gaaaataact tggaaaccct aaaatcaatc
     1201 tgtgaccagg tcaacaagag cctgctgggg aagatcgagg attatgaaag atcaagcaca
     1261 gagagaagaa tgagtcttga aggaagggaa gagttgccac cttcagtttt ggatgagatg
     1321 agcctcaaaa tggcggaact gtgtgactcg ccaagagaac aagacagtga gtcacggact
     1381 tcagacaaag tttaccaaat gaagaacaaa cctcggggat actgtctgat catcaacaat
     1441 catgatttca gcaaggcccg ggaagacata acccaactcc gaaaaatgaa ggacagaaaa
     1501 ggaacagact gtgataaaga ggctctgagt aagaccttta aggagcttca ttttgagata
     1561 gtatcttacg acgactgcac tgcaaatgaa atccacgaga ttctagaagg ctaccaaagc
     1621 gcagaccaca agaacaaaga ctgcttcatc tgctgtatct tatcccacgg tgacaagggt
     1681 gtcgtctatg gaacggatgg gaaggaggcc tccatctatg acctgacatc ttacttcact
     1741 ggttcaaagt gcccttccct gtctgggaaa cccaagatct ttttcattca ggcttgccaa
     1801 ggaagtaact tccagaaagg agtgcctgat gaggcaggct tcgagcaaca gaaccacact
     1861 ttagaagtgg attcatcatc tcacaagaac tatattccgg atgaggcaga ctttctgctg
     1921 ggaatggcta cggtgaagaa ctgcgtttcc taccgagatc ctgtgaatgg aacctggtat
     1981 attcagtcac tttgccagag cctgagggaa agatgtcctc aaggagatga cattcttagc
     2041 atcctgactg gcgtgaacta tgacgtgagc aataaagacg acaggaggaa caagggaaag
     2101 cagatgccac agcccacctt cacactacgg aagaagctct tcttccctcc cactagttat
     2161 ccgtacgacg taccagacta cgcataagaa aagtgaggat cctgagaact tcagggtgag
     2221 tttggggacc cttgattgtt ctttcttttt cgctattgta aaattcatgt tatatggagg
     2281 gggcaaagtt ttcagggtgt tgtttagaat gggaagatgt cccttgtatc accatggacc
     2341 ctcatgataa ttttgtttct ttcactttct actctgttga caaccattgt ctcctcttat
     2401 tttcttttca ttttctgtaa ctttttcgtt aaactttagc ttgcatttgt aacgaatttt
     2461 taaattcact tttgtttatt tgtcagattg taagtacttt ctctaatcac ttttttttca
     2521 aggcaatcag ggtatattat attgtacttc agcacagttt tagagaacaa ttgttataat
     2581 taaatgataa ggtagaatat ttctgcatat aaattctggc tggcgtggaa atattcttat
     2641 tggtagaaac aactacatcc tggtcatcat cctgcctttc tctttatggt tacaatgata
     2701 tacactgttt gagatgagga taaaatactc tgagtccaaa ccgggcccct ctgctaacca
     2761 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggttg ttgtgctgtc
     2821 tcatcatttt ggcaaagaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc
     2881 tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa
     2941 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
     3001 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     3061 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccat
     3121 atgctggctg ccatgaacaa aggtggctat aaagaggtca tcagtatatg aaacagcccc
     3181 ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat
     3241 tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag
     3301 ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgaa
     3361 gatccctcga ggagcttttt gcaaaagccc taggcctcca aaaaagcctc ttcactactt
     3421 ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
     3481 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
     3541 gggcgggact atggttgctg actaattgct gcattaatga atcggccaac gcgcggggag
     3601 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     3661 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     3721 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3781 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     3841 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3901 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     3961 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4021 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4081 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4141 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     4201 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     4261 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     4321 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     4381 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     4441 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     4501 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     4561 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     4621 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     4681 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     4741 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     4801 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     4861 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     4921 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     4981 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     5041 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     5101 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     5161 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     5221 gctcatcatt gccacctcca cctgaaaacg ttcttcgggg cgaaaactct caaggatctt
     5281 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     5341 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     5401 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     5461 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     5521 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     5581 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     5641 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     5701 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     5761 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     5821 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta
     5881 aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     5941 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     6001 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     6061 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     6121 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     6181 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     6241 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     6301 gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt
     6361 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     6421 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     6481 acggccagtg aattgtaata cgactcacta tagggcgaat tcgagctcgc cccgttacat
     6541 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     6601 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     6661 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     6721 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     6781 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     6841 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     6901 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     6961 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     7021 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
     7081 gctgttttga cctccataga agacaccggg accgatccag cctccggccc ctgggactcc
     7141 ttcttggtgg cgtgaaactc ccgcacctct tcggccagcg ccttgta
//
