LOCUS       V008423                 7106 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008423
VERSION     V008423
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7106)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7106)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7106)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7106)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..54
                     /label="myr"
                     /note="N-myristoylation signal from Src kinase (Pellman et
                     al., 1985; Kaplan et al., 1988)"
     CDS             61..381
                     /label="FKBP"
                     /note="human FK506-binding protein FKBP12"
     CDS             388..708
                     /codon_start=1
                     /note="unnamed protein product; hFKBP1A"
                     /protein_id="SJL87490.1"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             715..2070
                     /gene="Casp2"
                     /label="Caspase-2"
                     /note="Caspase-2 from Mus musculus. Accession#: P29594"
     CDS             2077..2103
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     intron          2135..2707
                     /label="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     CDS             2711..2833
                     /gene="HBB"
                     /label="Hemoglobin subunit beta"
                     /note="Hemoglobin subunit beta from Colobus guereza.
                     Accession#: Q9TT33"
     polyA_signal    2904..2959
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     rep_origin      complement(3312..3447)
                     /direction=LEFT
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     rep_origin      complement(3724..4312)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4483..5355)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin"
                     /protein_id="SJL87492.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFQVEVAMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDL
                     VEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTR
                     LDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAE
                     IGASLIKHW"
     promoter        complement(5356..5460)
                     /label="AmpR promoter"
     rep_origin      complement(5792..6247)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     6392..6408
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6415..6433
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        6452..6755
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6756..6959
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
ORIGIN
        1 gaattcgcgc gtatggggag tagcaagagc aagcctaagg accccagcca gcgctctaga
       61 ggagtgcagg tggaaaccat ctccccggga gacgggcgca ccttccccaa gcgcggccag
      121 acctgcgtgg tgcactacac cgggatgctt gaagatggaa agaaatttga ttcctcccgg
      181 gacagaaaca agccctttaa gtttatgcta ggcaagcagg aggtgatccg aggctgggaa
      241 gaaggggttg cccagatgag tgtgggtcag agagccaaac tgactatatc tccagattat
      301 gcctatggtg ccactgggca cccaggcatc atcccaccac atgccactct cgtcttcgat
      361 gtggagcttc taaaactgga agtcgagggc gtgcaggtgg aaaccatctc cccaggagac
      421 gggcgcacct tccccaagcg cggccagacc tgcgtggtgc actacaccgg gatgcttgaa
      481 gatggaaaga aatttgattc ctcccgggac agaaacaagc cctttaagtt tatgctaggc
      541 aagcaggagg tgatccgagg ctgggaagaa ggggttgccc agatgagtgt gggtcagaga
      601 gccaaactga ctatatctcc agattatgcc tatggtgcca ctgggcaccc aggcatcatc
      661 ccaccacatg ccactctcgt cttcgatgtg gagcttctaa aactggaaac tagtatggcg
      721 gcgccgagcg ggaggtcgca gtcctccctg cacaggaagg ggctgatggc ggctgacagg
      781 aggagcagga ttttggcagt gtgtggaatg catcctgacc accaggaaac actgaaaaag
      841 aatcgagtgg tgctggccaa gcagctgctg ctgagcgagc tgttagaaca cctcctagag
      901 aaggacatta tcactttgga aatgagggag ctcatccagg ccaaaggggg cagtttcagc
      961 cagaatgtgg aactcctcaa cctgctgcca aagagaggac cccaggcttt tgatgccttc
     1021 tgtgaagccc tgcgggagac caggcagggt cacttggaag acttactgct cacaaccctc
     1081 tcagatattc agcacgtact cccaccgttg agctgtgact atgacacaag tctccctttc
     1141 tcggtgtgtg agtcctgccc tcctcacaag cagctccgcc tatccacaga tgctacggaa
     1201 cactccttag ataatggtga tggtcctccc tgtcttctgg tgaagccatg cactcctgag
     1261 ttttaccagg cacactacca gctggcctat aggttgcaat ctcagccccg tggcttggca
     1321 ctggtgctga gcaatgtgca cttcactgga gagaaagacc tggaattccg ctctggaggg
     1381 gatgtggacc acactactct agtcaccctc ttcaagcttt tgggctacaa tgtccatgtg
     1441 ctacatgacc agaccgcaca ggaaatgcaa gagaaacttc agaattttgc acagttacct
     1501 gcacaccggg tcacagactc ctgcgtagtg gcactcctct cacatggtgt ggaaggtggc
     1561 atctatggtg tagatggcaa actgcttcag ctccaagagg tttttcgact ttttgacaat
     1621 gctaactgtc caagtctaca gaacaagcca aaaatgttct tcatccaagc atgtcgtgga
     1681 gatgagacag atagaggtgt cgaccagcaa gatggaaaga accacacaca atcccctgga
     1741 tgtgaggaga gtgatgctgg caaagaggag ttgatgaaga tgagactgcc tactcgctca
     1801 gacatgatat gtggctatgc ttgccttaaa ggtaatgctg ccatgcggaa caccaaacgg
     1861 ggttcctggt acattgaggc cctcactcag gtgttctctg aaagagcttg tgacatgcac
     1921 gtggccgaca tgcttgttaa ggtgaatgcc cttatcaagg agcgtgaagg ctatgcccct
     1981 ggcacagaat tccaccgatg caaggagatg tctgagtact gtagtactct gtgccagcaa
     2041 ctctacctgt tcccaggcta cccacccacg tctagttatc cgtacgacgt accagactac
     2101 gcataagaaa agtgaggatc ctgagaactt cagggtgagt ttggggaccc ttgattgttc
     2161 tttctttttc gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt
     2221 gtttagaatg ggaagatgtc ccttgtatca ccatggaccc tcatgataat tttgtttctt
     2281 tcactttcta ctctgttgac aaccattgtc tcctcttatt ttcttttcat tttctgtaac
     2341 tttttcgtta aactttagct tgcatttgta acgaattttt aaattcactt ttgtttattt
     2401 gtcagattgt aagtactttc tctaatcact tttttttcaa ggcaatcagg gtatattata
     2461 ttgtacttca gcacagtttt agagaacaat tgttataatt aaatgataag gtagaatatt
     2521 tctgcatata aattctggct ggcgtggaaa tattcttatt ggtagaaaca actacatcct
     2581 ggtcatcatc ctgcctttct ctttatggtt acaatgatat acactgtttg agatgaggat
     2641 aaaatactct gagtccaaac cgggcccctc tgctaaccat gttcatgcct tcttcttttt
     2701 cctacagctc ctgggcaacg tgctggttgt tgtgctgtct catcattttg gcaaagaatt
     2761 cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc
     2821 tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc
     2881 ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     2941 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca
     3001 gaatgagtat ttggtttaga gtttggcaac atatgccata tgctggctgc catgaacaaa
     3061 ggtggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc
     3121 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt
     3181 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct
     3241 gactactccc agtcatagct gtccctcttc tcttatgaag atccctcgag gagctttttg
     3301 caaaagccct aggcctccaa aaaagcctct tcactacttc tggaatagct cagaggccga
     3361 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
     3421 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga
     3481 ctaattgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     3541 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     3601 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     3661 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3721 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3781 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3841 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3901 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3961 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4021 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4081 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4141 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     4201 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     4261 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4321 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4381 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4441 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     4501 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     4561 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     4621 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     4681 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     4741 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     4801 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     4861 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     4921 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     4981 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     5041 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     5101 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg ccacctccac
     5161 ctgaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     5221 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     5281 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     5341 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     5401 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     5461 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     5521 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     5581 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     5641 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     5701 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     5761 cagatgcgta aggagaaaat accgcatcag gaaattgtaa acgttaatat tttgttaaaa
     5821 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     5881 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     5941 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     6001 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     6061 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     6121 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     6181 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     6241 ggcgcgtcgc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     6301 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     6361 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac
     6421 gactcactat agggcgaatt cgagctcgcc ccgttacata acttacggta aatggcccgc
     6481 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     6541 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     6601 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     6661 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     6721 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
     6781 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     6841 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
     6901 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
     6961 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
     7021 gacaccggga ccgatccagc ctccggcccc tgggactcct tcttggtggc gtgaaactcc
     7081 cgcacctctt cggccagcgc cttgta
//
