LOCUS       V008424                 7208 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008424
VERSION     V008424
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7208)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7208)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7208)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..54
                     /label="myr"
                     /note="N-myristoylation signal from Src kinase (Pellman et
                     al., 1985; Kaplan et al., 1988)"
     CDS             61..381
                     /label="FKBP"
                     /note="human FK506-binding protein FKBP12"
     CDS             388..708
                     /codon_start=1
                     /note="unnamed protein product; hFKBP1A"
                     /protein_id="SJL87500.1"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     misc_feature    715..732
                     /label="GSGGGS linker"
                     /note="GSGGGS linker"
     CDS             733..2172
                     /gene="Casp8"
                     /label="Caspase-8"
                     /note="Caspase-8 from Mus musculus. Accession#: O89110"
     CDS             2179..2205
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     intron          2237..2809
                     /label="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     CDS             2813..2935
                     /gene="HBB"
                     /label="Hemoglobin subunit beta"
                     /note="Hemoglobin subunit beta from Colobus guereza.
                     Accession#: Q9TT33"
     polyA_signal    3006..3061
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     rep_origin      complement(3414..3549)
                     /direction=LEFT
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     rep_origin      complement(3826..4414)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4585..5457)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin"
                     /protein_id="SJL87502.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFQVEVAMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDL
                     VEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTR
                     LDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAE
                     IGASLIKHW"
     promoter        complement(5458..5562)
                     /label="AmpR promoter"
     rep_origin      complement(5894..6349)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     6494..6510
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6517..6535
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        6554..6857
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6858..7061
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
ORIGIN
        1 gaattcgcgc gtatggggag tagcaagagc aagcctaagg accccagcca gcgctctaga
       61 ggagtgcagg tggaaaccat ctccccggga gacgggcgca ccttccccaa gcgcggccag
      121 acctgcgtgg tgcactacac cgggatgctt gaagatggaa agaaatttga ttcctcccgg
      181 gacagaaaca agccctttaa gtttatgcta ggcaagcagg aggtgatccg aggctgggaa
      241 gaaggggttg cccagatgag tgtgggtcag agagccaaac tgactatatc tccagattat
      301 gcctatggtg ccactgggca cccaggcatc atcccaccac atgccactct cgtcttcgat
      361 gtggagcttc taaaactgga agtcgagggc gtgcaggtgg aaaccatctc cccaggagac
      421 gggcgcacct tccccaagcg cggccagacc tgcgtggtgc actacaccgg gatgcttgaa
      481 gatggaaaga aatttgattc ctcccgggac agaaacaagc cctttaagtt tatgctaggc
      541 aagcaggagg tgatccgagg ctgggaagaa ggggttgccc agatgagtgt gggtcagaga
      601 gccaaactga ctatatctcc agattatgcc tatggtgcca ctgggcaccc aggcatcatc
      661 ccaccacatg ccactctcgt cttcgatgtg gagcttctaa aactggaaac tagtgggtct
      721 ggaggcggat ccatggattt ccagagttgt ctttatgcta ttgctgaaga actgggcagt
      781 gaagacctgg ctgccctcaa gttcctgtgc ttggactaca tcccacacaa gaagcaggag
      841 accatcgagg atgcccagaa gctatttctg aggcttcggg aaaaggggat gttggaggaa
      901 ggcaatctgt ctttcctgaa agagctgctt ttccacatca gtcggtggga cctgctggtc
      961 aacttcctag actgcaaccg agaggagatg gtgagagagc tgcgggaccc agacaatgtc
     1021 cagatttctc cctacagggt catgctcttt aagctctcag aagaagtgag cgagttggaa
     1081 ttgagatctt ttaagttcct tttgaacaat gagatcccca aatgtaagct ggaagatgac
     1141 ttgagcctgc ttgaaatttt tgtagaaatg gagaagagga ccatgctggc agaaaataac
     1201 ttggaaaccc taaaatcaat ctgtgaccag gtcaacaaga gcctgctggg gaagatcgag
     1261 gattatgaaa gatcaagcac agagagaaga atgagtcttg aaggaaggga agagttgcca
     1321 ccttcagttt tggatgagat gagcctcaaa atggcggaac tgtgtgactc gccaagagaa
     1381 caagacagtg agtcacggac ttcagacaaa gtttaccaaa tgaagaacaa acctcgggga
     1441 tactgtctga tcatcaacaa tcatgatttc agcaaggccc gggaagacat aacccaactc
     1501 cgaaaaatga aggacagaaa aggaacagac tgtgataaag aggctctgag taagaccttt
     1561 aaggagcttc attttgagat agtatcttac gacgactgca ctgcaaatga aatccacgag
     1621 attctagaag gctaccaaag cgcagaccac aagaacaaag actgcttcat ctgctgtatc
     1681 ttatcccacg gtgacaaggg tgtcgtctat ggaacggatg ggaaggaggc ctccatctat
     1741 gacctgacat cttacttcac tggttcaaag tgcccttccc tgtctgggaa acccaagatc
     1801 tttttcattc aggcttgcca aggaagtaac ttccagaaag gagtgcctga tgaggcaggc
     1861 ttcgagcaac agaaccacac tttagaagtg gattcatcat ctcacaagaa ctatattccg
     1921 gatgaggcag actttctgct gggaatggct acggtgaaga actgcgtttc ctaccgagat
     1981 cctgtgaatg gaacctggta tattcagtca ctttgccaga gcctgaggga aagatgtcct
     2041 caaggagatg acattcttag catcctgact ggcgtgaact atgacgtgag caataaagac
     2101 gacaggagga acaagggaaa gcagatgcca cagcccacct tcacactacg gaagaagctc
     2161 ttcttccctc ccactagtta tccgtacgac gtaccagact acgcataaga aaagtgagga
     2221 tcctgagaac ttcagggtga gtttggggac ccttgattgt tctttctttt tcgctattgt
     2281 aaaattcatg ttatatggag ggggcaaagt tttcagggtg ttgtttagaa tgggaagatg
     2341 tcccttgtat caccatggac cctcatgata attttgtttc tttcactttc tactctgttg
     2401 acaaccattg tctcctctta ttttcttttc attttctgta actttttcgt taaactttag
     2461 cttgcatttg taacgaattt ttaaattcac ttttgtttat ttgtcagatt gtaagtactt
     2521 tctctaatca cttttttttc aaggcaatca gggtatatta tattgtactt cagcacagtt
     2581 ttagagaaca attgttataa ttaaatgata aggtagaata tttctgcata taaattctgg
     2641 ctggcgtgga aatattctta ttggtagaaa caactacatc ctggtcatca tcctgccttt
     2701 ctctttatgg ttacaatgat atacactgtt tgagatgagg ataaaatact ctgagtccaa
     2761 accgggcccc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa
     2821 cgtgctggtt gttgtgctgt ctcatcattt tggcaaagaa ttcactcctc aggtgcaggc
     2881 tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga
     2941 tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc
     3001 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
     3061 actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta
     3121 gagtttggca acatatgcca tatgctggct gccatgaaca aaggtggcta taaagaggtc
     3181 atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt
     3241 gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat
     3301 tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag
     3361 ctgtccctct tctcttatga agatccctcg aggagctttt tgcaaaagcc ctaggcctcc
     3421 aaaaaagcct cttcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc
     3481 ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag
     3541 gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattgc tgcattaatg
     3601 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     3661 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     3721 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     3781 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3841 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3901 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3961 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     4021 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     4081 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4141 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     4201 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     4261 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     4321 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     4381 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     4441 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     4501 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     4561 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     4621 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     4681 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     4741 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     4801 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     4861 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     4921 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     4981 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     5041 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     5101 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     5161 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     5221 acatagcaga actttaaaag tgctcatcat tgccacctcc acctgaaaac gttcttcggg
     5281 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     5341 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     5401 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     5461 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     5521 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     5581 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     5641 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     5701 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     5761 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     5821 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     5881 ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt
     5941 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     6001 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     6061 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     6121 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     6181 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     6241 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     6301 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg
     6361 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     6421 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     6481 cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa
     6541 ttcgagctcg ccccgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     6601 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     6661 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     6721 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     6781 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     6841 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     6901 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     6961 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     7021 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
     7081 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
     7141 gcctccggcc cctgggactc cttcttggtg gcgtgaaact cccgcacctc ttcggccagc
     7201 gccttgta
//
