LOCUS       V008425                 6398 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008425
VERSION     V008425
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6398)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6398)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6398)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..54
                     /label="myr"
                     /note="N-myristoylation signal from Src kinase (Pellman et
                     al., 1985; Kaplan et al., 1988)"
     CDS             61..381
                     /label="FKBP"
                     /note="human FK506-binding protein FKBP12"
     CDS             388..708
                     /codon_start=1
                     /note="unnamed protein product; hFKBP1A"
                     /protein_id="SJL87538.1"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     misc_feature    715..732
                     /label="GSGGGS linker"
                     /note="GSGGGS linker"
     CDS             739..1362
                     /gene="FADD"
                     /label="FAS-associated death domain protein"
                     /note="FAS-associated death domain protein from Homo
                     sapiens. Accession#: Q13158"
     CDS             1369..1395
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     intron          1427..1999
                     /label="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     CDS             2003..2125
                     /gene="HBB"
                     /label="Hemoglobin subunit beta"
                     /note="Hemoglobin subunit beta from Colobus guereza.
                     Accession#: Q9TT33"
     polyA_signal    2196..2251
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     rep_origin      complement(2604..2739)
                     /direction=LEFT
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     rep_origin      complement(3016..3604)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3775..4647)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin"
                     /protein_id="SJL87540.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFQVEVAMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDL
                     VEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTR
                     LDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAE
                     IGASLIKHW"
     promoter        complement(4648..4752)
                     /label="AmpR promoter"
     rep_origin      complement(5084..5539)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5684..5700
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5707..5725
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        5744..6047
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6048..6251
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
ORIGIN
        1 gaattcgcgc gtatggggag tagcaagagc aagcctaagg accccagcca gcgctctaga
       61 ggagtgcagg tggaaaccat ctccccggga gacgggcgca ccttccccaa gcgcggccag
      121 acctgcgtgg tgcactacac cgggatgctt gaagatggaa agaaatttga ttcctcccgg
      181 gacagaaaca agccctttaa gtttatgcta ggcaagcagg aggtgatccg aggctgggaa
      241 gaaggggttg cccagatgag tgtgggtcag agagccaaac tgactatatc tccagattat
      301 gcctatggtg ccactgggca cccaggcatc atcccaccac atgccactct cgtcttcgat
      361 gtggagcttc taaaactgga agtcgagggc gtgcaggtgg aaaccatctc cccaggagac
      421 gggcgcacct tccccaagcg cggccagacc tgcgtggtgc actacaccgg gatgcttgaa
      481 gatggaaaga aatttgattc ctcccgggac agaaacaagc cctttaagtt tatgctaggc
      541 aagcaggagg tgatccgagg ctgggaagaa ggggttgccc agatgagtgt gggtcagaga
      601 gccaaactga ctatatctcc agattatgcc tatggtgcca ctgggcaccc aggcatcatc
      661 ccaccacatg ccactctcgt cttcgatgtg gagcttctaa aactggaaac tagagggtct
      721 ggaggcggat cctctagtat ggacccgttc ctggtgctgc tgcactcggt gtcgtccagc
      781 ctgtcgagca gcgagctgac cgagctcaag ttcctatgcc tcgggcgcgt gggcaagcgc
      841 aagctggagc gcgtgcagag cggcctagac ctcttctcca tgctgctgga gcagaacgac
      901 ctggagcccg ggcacaccga gctcctgcgc gagctgctcg cctccctgcg gcgccacgac
      961 ctgctgcggc gcgtcgacga cttcgaggcg ggggcggcgg ccggggccgc gcctggggaa
     1021 gaagacctgt gtgcagcatt taacgtcata tgtgataatg tggggaaaga ttggagaagg
     1081 ctggctcgtc agctcaaagt ctcagacacc aagatcgaca gcatcgagga cagatacccc
     1141 cgcaacctga cagagcgtgt gcgggagtca ctgagaatct ggaagaacac agagaaggag
     1201 aacgcaacag tggcccacct ggtgggggct ctcaggtcct gccagatgaa cctggtggct
     1261 gacctggtac aagaggttca gcaggcccgt gacctccaga acaggagtgg ggccatgtcc
     1321 ccgatgtcat ggaactcaga cgcatctacc tccgaagcgt cctctagtta tccgtacgac
     1381 gtaccagact acgcataaga aaagtgagga tcctgagaac ttcagggtga gtttggggac
     1441 ccttgattgt tctttctttt tcgctattgt aaaattcatg ttatatggag ggggcaaagt
     1501 tttcagggtg ttgtttagaa tgggaagatg tcccttgtat caccatggac cctcatgata
     1561 attttgtttc tttcactttc tactctgttg acaaccattg tctcctctta ttttcttttc
     1621 attttctgta actttttcgt taaactttag cttgcatttg taacgaattt ttaaattcac
     1681 ttttgtttat ttgtcagatt gtaagtactt tctctaatca cttttttttc aaggcaatca
     1741 gggtatatta tattgtactt cagcacagtt ttagagaaca attgttataa ttaaatgata
     1801 aggtagaata tttctgcata taaattctgg ctggcgtgga aatattctta ttggtagaaa
     1861 caactacatc ctggtcatca tcctgccttt ctctttatgg ttacaatgat atacactgtt
     1921 tgagatgagg ataaaatact ctgagtccaa accgggcccc tctgctaacc atgttcatgc
     1981 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt gttgtgctgt ctcatcattt
     2041 tggcaaagaa ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc
     2101 caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga
     2161 catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc
     2221 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     2281 tttaaaacat cagaatgagt atttggttta gagtttggca acatatgcca tatgctggct
     2341 gccatgaaca aaggtggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc
     2401 attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg
     2461 tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt
     2521 tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatga agatccctcg
     2581 aggagctttt tgcaaaagcc ctaggcctcc aaaaaagcct cttcactact tctggaatag
     2641 ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg
     2701 cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac
     2761 tatggttgct gactaattgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     2821 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     2881 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     2941 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3001 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3061 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3121 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3181 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3241 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3301 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3361 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3421 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     3481 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3541 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3601 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     3661 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     3721 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     3781 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     3841 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     3901 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     3961 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     4021 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     4081 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     4141 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     4201 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     4261 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     4321 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     4381 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     4441 tgccacctcc acctgaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     4501 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     4561 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     4621 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     4681 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     4741 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     4801 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     4861 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     4921 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     4981 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     5041 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt aaacgttaat
     5101 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     5161 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     5221 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     5281 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     5341 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     5401 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     5461 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     5521 gcgccgctac agggcgcgtc gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg
     5581 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     5641 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     5701 gaattgtaat acgactcact atagggcgaa ttcgagctcg ccccgttaca taacttacgg
     5761 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     5821 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     5881 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     5941 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     6001 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     6061 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     6121 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     6181 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     6241 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     6301 acctccatag aagacaccgg gaccgatcca gcctccggcc cctgggactc cttcttggtg
     6361 gcgtgaaact cccgcacctc ttcggccagc gccttgta
//
