LOCUS       V008426                 6347 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008426
VERSION     V008426
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6347)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6347)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6347)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..54
                     /label="myr"
                     /note="N-myristoylation signal from Src kinase (Pellman et
                     al., 1985; Kaplan et al., 1988)"
     CDS             61..381
                     /label="FKBP"
                     /note="human FK506-binding protein FKBP12"
     CDS             388..708
                     /codon_start=1
                     /note="unnamed protein product; hFKBP1A"
                     /protein_id="SJL87485.1"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             715..1311
                     /gene="CRADD"
                     /label="Death domain-containing protein CRADD"
                     /note="Death domain-containing protein CRADD from Homo
                     sapiens. Accession#: P78560"
     CDS             1318..1344
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     intron          1376..1948
                     /label="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     CDS             1952..2074
                     /gene="HBB"
                     /label="Hemoglobin subunit beta"
                     /note="Hemoglobin subunit beta from Colobus guereza.
                     Accession#: Q9TT33"
     polyA_signal    2145..2200
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     rep_origin      complement(2553..2688)
                     /direction=LEFT
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     rep_origin      complement(2965..3553)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3724..4596)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin"
                     /protein_id="SJL87487.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFQVEVAMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDL
                     VEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTR
                     LDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAE
                     IGASLIKHW"
     promoter        complement(4597..4701)
                     /label="AmpR promoter"
     rep_origin      complement(5033..5488)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5633..5649
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5656..5674
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        5693..5996
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5997..6200
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
ORIGIN
        1 gaattcgcgc gtatggggag tagcaagagc aagcctaagg accccagcca gcgctctaga
       61 ggagtgcagg tggaaaccat ctccccggga gacgggcgca ccttccccaa gcgcggccag
      121 acctgcgtgg tgcactacac cgggatgctt gaagatggaa agaaatttga ttcctcccgg
      181 gacagaaaca agccctttaa gtttatgcta ggcaagcagg aggtgatccg aggctgggaa
      241 gaaggggttg cccagatgag tgtgggtcag agagccaaac tgactatatc tccagattat
      301 gcctatggtg ccactgggca cccaggcatc atcccaccac atgccactct cgtcttcgat
      361 gtggagcttc taaaactgga agtcgagggc gtgcaggtgg aaaccatctc cccaggagac
      421 gggcgcacct tccccaagcg cggccagacc tgcgtggtgc actacaccgg gatgcttgaa
      481 gatggaaaga aatttgattc ctcccgggac agaaacaagc cctttaagtt tatgctaggc
      541 aagcaggagg tgatccgagg ctgggaagaa ggggttgccc agatgagtgt gggtcagaga
      601 gccaaactga ctatatctcc agattatgcc tatggtgcca ctgggcaccc aggcatcatc
      661 ccaccacatg ccactctcgt cttcgatgtg gagcttctaa aactggaaac tagtatggag
      721 gccagagaca aacaagtact ccgctcactt cgcctggagc tgggtgcaga ggtattggtg
      781 gagggactgg ttcttcagta cctctaccag gaaggaatct tgacggaaaa ccatattcaa
      841 gaaatcaatg ctcaaaccac aggcctccgg aaaacaatgc tcctgctgga tatcctacct
      901 tccaggggcc ctaaagcatt tgatacattc ctagattccc tacaggagtt tccctgggtc
      961 agggagaagc tgaagaaggc aagggaagag gccatgaccg acctgcctgc aggtgacaga
     1021 ttgactggga tcccctcgca catcctcaac agctccccat cagaccggca gattaaccag
     1081 ctggcccaga ggctgggccc tgagtgggag cccatggtgc tgtctctggg actgtcccag
     1141 acggatatct accgctgtaa ggccaaccac ccccacaacg tgcagtcgca ggtggtggag
     1201 gccttcatcc gttggcggca gcgcttcggg aagcaggcca ccttccagag cctgcacaac
     1261 gggctgcggg ctgtggaggt ggacccctcg ctgctcctgc acatgttgga gactagttat
     1321 ccgtacgacg taccagacta cgcataagaa aagtgaggat cctgagaact tcagggtgag
     1381 tttggggacc cttgattgtt ctttcttttt cgctattgta aaattcatgt tatatggagg
     1441 gggcaaagtt ttcagggtgt tgtttagaat gggaagatgt cccttgtatc accatggacc
     1501 ctcatgataa ttttgtttct ttcactttct actctgttga caaccattgt ctcctcttat
     1561 tttcttttca ttttctgtaa ctttttcgtt aaactttagc ttgcatttgt aacgaatttt
     1621 taaattcact tttgtttatt tgtcagattg taagtacttt ctctaatcac ttttttttca
     1681 aggcaatcag ggtatattat attgtacttc agcacagttt tagagaacaa ttgttataat
     1741 taaatgataa ggtagaatat ttctgcatat aaattctggc tggcgtggaa atattcttat
     1801 tggtagaaac aactacatcc tggtcatcat cctgcctttc tctttatggt tacaatgata
     1861 tacactgttt gagatgagga taaaatactc tgagtccaaa ccgggcccct ctgctaacca
     1921 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggttg ttgtgctgtc
     1981 tcatcatttt ggcaaagaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc
     2041 tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa
     2101 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
     2161 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     2221 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccat
     2281 atgctggctg ccatgaacaa aggtggctat aaagaggtca tcagtatatg aaacagcccc
     2341 ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat
     2401 tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag
     2461 ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgaa
     2521 gatccctcga ggagcttttt gcaaaagccc taggcctcca aaaaagcctc ttcactactt
     2581 ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
     2641 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
     2701 gggcgggact atggttgctg actaattgct gcattaatga atcggccaac gcgcggggag
     2761 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     2821 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     2881 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     2941 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     3001 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3061 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     3121 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     3181 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     3241 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     3301 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3361 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     3421 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     3481 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3541 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     3601 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3661 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     3721 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     3781 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     3841 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     3901 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     3961 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     4021 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     4081 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     4141 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     4201 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     4261 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     4321 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     4381 gctcatcatt gccacctcca cctgaaaacg ttcttcgggg cgaaaactct caaggatctt
     4441 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     4501 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     4561 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     4621 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     4681 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     4741 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     4801 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     4861 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     4921 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     4981 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta
     5041 aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     5101 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     5161 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     5221 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     5281 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     5341 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     5401 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     5461 gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt
     5521 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     5581 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     5641 acggccagtg aattgtaata cgactcacta tagggcgaat tcgagctcgc cccgttacat
     5701 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     5761 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     5821 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     5881 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     5941 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     6001 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     6061 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     6121 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     6181 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
     6241 gctgttttga cctccataga agacaccggg accgatccag cctccggccc ctgggactcc
     6301 ttcttggtgg cgtgaaactc ccgcacctct tcggccagcg ccttgta
//
