LOCUS       V008427                 6374 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008427
VERSION     V008427
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6374)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6374)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6374)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6374
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..54
                     /label="myr"
                     /note="N-myristoylation signal from Src kinase (Pellman et
                     al., 1985; Kaplan et al., 1988)"
     CDS             61..381
                     /label="FKBP"
                     /note="human FK506-binding protein FKBP12"
     CDS             388..708
                     /codon_start=1
                     /note="unnamed protein product; hFKBP1A"
                     /protein_id="SJL87475.1"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             715..1338
                     /gene="FADD"
                     /label="FAS-associated death domain protein"
                     /note="FAS-associated death domain protein from Homo
                     sapiens. Accession#: Q13158"
     CDS             1345..1371
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     intron          1403..1975
                     /label="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     CDS             1979..2101
                     /gene="HBB"
                     /label="Hemoglobin subunit beta"
                     /note="Hemoglobin subunit beta from Colobus guereza.
                     Accession#: Q9TT33"
     polyA_signal    2172..2227
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     rep_origin      complement(2580..2715)
                     /direction=LEFT
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     rep_origin      complement(2992..3580)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3751..4623)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin"
                     /protein_id="SJL87477.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFQVEVAMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDL
                     VEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTR
                     LDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAE
                     IGASLIKHW"
     promoter        complement(4624..4728)
                     /label="AmpR promoter"
     rep_origin      complement(5060..5515)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5660..5676
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5683..5701
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        5720..6023
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6024..6227
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
ORIGIN
        1 gaattcgcgc gtatggggag tagcaagagc aagcctaagg accccagcca gcgctctaga
       61 ggagtgcagg tggaaaccat ctccccggga gacgggcgca ccttccccaa gcgcggccag
      121 acctgcgtgg tgcactacac cgggatgctt gaagatggaa agaaatttga ttcctcccgg
      181 gacagaaaca agccctttaa gtttatgcta ggcaagcagg aggtgatccg aggctgggaa
      241 gaaggggttg cccagatgag tgtgggtcag agagccaaac tgactatatc tccagattat
      301 gcctatggtg ccactgggca cccaggcatc atcccaccac atgccactct cgtcttcgat
      361 gtggagcttc taaaactgga agtcgagggc gtgcaggtgg aaaccatctc cccaggagac
      421 gggcgcacct tccccaagcg cggccagacc tgcgtggtgc actacaccgg gatgcttgaa
      481 gatggaaaga aatttgattc ctcccgggac agaaacaagc cctttaagtt tatgctaggc
      541 aagcaggagg tgatccgagg ctgggaagaa ggggttgccc agatgagtgt gggtcagaga
      601 gccaaactga ctatatctcc agattatgcc tatggtgcca ctgggcaccc aggcatcatc
      661 ccaccacatg ccactctcgt cttcgatgtg gagcttctaa aactggaaac tagtatggac
      721 ccgttcctgg tgctgctgca ctcggtgtcg tccagcctgt cgagcagcga gctgaccgag
      781 ctcaagttcc tatgcctcgg gcgcgtgggc aagcgcaagc tggagcgcgt gcagagcggc
      841 ctagacctct tctccatgct gctggagcag aacgacctgg agcccgggca caccgagctc
      901 ctgcgcgagc tgctcgcctc cctgcggcgc cacgacctgc tgcggcgcgt cgacgacttc
      961 gaggcggggg cggcggccgg ggccgcgcct ggggaagaag acctgtgtgc agcatttaac
     1021 gtcatatgtg ataatgtggg gaaagattgg agaaggctgg ctcgtcagct caaagtctca
     1081 gacaccaaga tcgacagcat cgaggacaga tacccccgca acctgacaga gcgtgtgcgg
     1141 gagtcactga gaatctggaa gaacacagag aaggagaacg caacagtggc ccacctggtg
     1201 ggggctctca ggtcctgcca gatgaacctg gtggctgacc tggtacaaga ggttcagcag
     1261 gcccgtgacc tccagaacag gagtggggcc atgtccccga tgtcatggaa ctcagacgca
     1321 tctacctccg aagcgtcctc tagttatccg tacgacgtac cagactacgc ataagaaaag
     1381 tgaggatcct gagaacttca gggtgagttt ggggaccctt gattgttctt tctttttcgc
     1441 tattgtaaaa ttcatgttat atggaggggg caaagttttc agggtgttgt ttagaatggg
     1501 aagatgtccc ttgtatcacc atggaccctc atgataattt tgtttctttc actttctact
     1561 ctgttgacaa ccattgtctc ctcttatttt cttttcattt tctgtaactt tttcgttaaa
     1621 ctttagcttg catttgtaac gaatttttaa attcactttt gtttatttgt cagattgtaa
     1681 gtactttctc taatcacttt tttttcaagg caatcagggt atattatatt gtacttcagc
     1741 acagttttag agaacaattg ttataattaa atgataaggt agaatatttc tgcatataaa
     1801 ttctggctgg cgtggaaata ttcttattgg tagaaacaac tacatcctgg tcatcatcct
     1861 gcctttctct ttatggttac aatgatatac actgtttgag atgaggataa aatactctga
     1921 gtccaaaccg ggcccctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
     1981 gggcaacgtg ctggttgttg tgctgtctca tcattttggc aaagaattca ctcctcaggt
     2041 gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca
     2101 ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct
     2161 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     2221 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt
     2281 ggtttagagt ttggcaacat atgccatatg ctggctgcca tgaacaaagg tggctataaa
     2341 gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct
     2401 tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc
     2461 taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag
     2521 tcatagctgt ccctcttctc ttatgaagat ccctcgagga gctttttgca aaagccctag
     2581 gcctccaaaa aagcctcttc actacttctg gaatagctca gaggccgagg cggcctcggc
     2641 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
     2701 agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact aattgctgca
     2761 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     2821 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     2881 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     2941 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     3001 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     3061 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     3121 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     3181 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     3241 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     3301 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     3361 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     3421 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     3481 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     3541 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     3601 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     3661 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     3721 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     3781 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     3841 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     3901 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     3961 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     4021 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     4081 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     4141 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     4201 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     4261 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     4321 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     4381 cgcgccacat agcagaactt taaaagtgct catcattgcc acctccacct gaaaacgttc
     4441 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     4501 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     4561 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     4621 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     4681 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     4741 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     4801 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     4861 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     4921 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     4981 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     5041 gagaaaatac cgcatcagga aattgtaaac gttaatattt tgttaaaatt cgcgttaaat
     5101 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     5161 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     5221 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     5281 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     5341 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     5401 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     5461 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcgcgc
     5521 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     5581 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     5641 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga ctcactatag
     5701 ggcgaattcg agctcgcccc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     5761 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     5821 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     5881 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     5941 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     6001 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     6061 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     6121 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     6181 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
     6241 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc
     6301 gatccagcct ccggcccctg ggactccttc ttggtggcgt gaaactcccg cacctcttcg
     6361 gccagcgcct tgta
//
