LOCUS       V008428                 6137 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008428
VERSION     V008428
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6137)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6137)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6137)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6137)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6137
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..54
                     /label="myr"
                     /note="N-myristoylation signal from Src kinase (Pellman et
                     al., 1985; Kaplan et al., 1988)"
     CDS             61..381
                     /label="FKBP"
                     /note="human FK506-binding protein FKBP12"
     CDS             388..708
                     /codon_start=1
                     /note="unnamed protein product; hFKBP1A"
                     /protein_id="SJL87480.1"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             715..1101
                     /codon_start=1
                     /note="unnamed protein product; hFADD-DN"
                     /protein_id="SJL87481.1"
                     /translation="DDFEAGAAAGAAPGEEDLCAAFNVICDNVGKDWRRLARQLKVSDT
                     KIDSIEDRYPRNLTERVRESLRIWKNTEKENATVAHLVGALRSCQMNLVADLVQEVQQA
                     RDLQNRSGAMSPMSWNSDASTSEAS"
     CDS             1108..1134
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     intron          1166..1738
                     /label="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     CDS             1742..1864
                     /gene="HBB"
                     /label="Hemoglobin subunit beta"
                     /note="Hemoglobin subunit beta from Colobus guereza.
                     Accession#: Q9TT33"
     polyA_signal    1935..1990
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     rep_origin      complement(2343..2478)
                     /direction=LEFT
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     rep_origin      complement(2755..3343)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3514..4386)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL87482.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFQVEVAMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDL
                     VEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTR
                     LDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAE
                     IGASLIKHW"
     promoter        complement(4387..4491)
                     /label="AmpR promoter"
     rep_origin      complement(4823..5278)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5423..5439
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5446..5464
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        5483..5786
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5787..5990
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
ORIGIN
        1 gaattcgcgc gtatggggag tagcaagagc aagcctaagg accccagcca gcgctctaga
       61 ggagtgcagg tggaaaccat ctccccggga gacgggcgca ccttccccaa gcgcggccag
      121 acctgcgtgg tgcactacac cgggatgctt gaagatggaa agaaatttga ttcctcccgg
      181 gacagaaaca agccctttaa gtttatgcta ggcaagcagg aggtgatccg aggctgggaa
      241 gaaggggttg cccagatgag tgtgggtcag agagccaaac tgactatatc tccagattat
      301 gcctatggtg ccactgggca cccaggcatc atcccaccac atgccactct cgtcttcgat
      361 gtggagcttc taaaactgga agtcgagggc gtgcaggtgg aaaccatctc cccaggagac
      421 gggcgcacct tccccaagcg cggccagacc tgcgtggtgc actacaccgg gatgcttgaa
      481 gatggaaaga aatttgattc ctcccgggac agaaacaagc cctttaagtt tatgctaggc
      541 aagcaggagg tgatccgagg ctgggaagaa ggggttgccc agatgagtgt gggtcagaga
      601 gccaaactga ctatatctcc agattatgcc tatggtgcca ctgggcaccc aggcatcatc
      661 ccaccacatg ccactctcgt cttcgatgtg gagcttctaa aactggaaac tagtgacgac
      721 ttcgaggcgg gggcggcggc cggggccgcg cctggggaag aagacctgtg tgcagcattt
      781 aacgtcatat gtgataatgt ggggaaagat tggagaaggc tggctcgtca gctcaaagtc
      841 tcagacacca agatcgacag catcgaggac agataccccc gcaacctgac agagcgtgtg
      901 cgggagtcac tgagaatctg gaagaacaca gagaaggaga acgcaacagt ggcccacctg
      961 gtgggggctc tcaggtcctg ccagatgaac ctggtggctg acctggtaca agaggttcag
     1021 caggcccgtg acctccagaa caggagtggg gccatgtccc cgatgtcatg gaactcagac
     1081 gcatctacct ccgaagcgtc ctctagttat ccgtacgacg taccagacta cgcataagaa
     1141 aagtgaggat cctgagaact tcagggtgag tttggggacc cttgattgtt ctttcttttt
     1201 cgctattgta aaattcatgt tatatggagg gggcaaagtt ttcagggtgt tgtttagaat
     1261 gggaagatgt cccttgtatc accatggacc ctcatgataa ttttgtttct ttcactttct
     1321 actctgttga caaccattgt ctcctcttat tttcttttca ttttctgtaa ctttttcgtt
     1381 aaactttagc ttgcatttgt aacgaatttt taaattcact tttgtttatt tgtcagattg
     1441 taagtacttt ctctaatcac ttttttttca aggcaatcag ggtatattat attgtacttc
     1501 agcacagttt tagagaacaa ttgttataat taaatgataa ggtagaatat ttctgcatat
     1561 aaattctggc tggcgtggaa atattcttat tggtagaaac aactacatcc tggtcatcat
     1621 cctgcctttc tctttatggt tacaatgata tacactgttt gagatgagga taaaatactc
     1681 tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1741 cctgggcaac gtgctggttg ttgtgctgtc tcatcatttt ggcaaagaat tcactcctca
     1801 ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata
     1861 ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca
     1921 tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     1981 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta
     2041 tttggtttag agtttggcaa catatgccat atgctggctg ccatgaacaa aggtggctat
     2101 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     2161 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
     2221 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
     2281 cagtcatagc tgtccctctt ctcttatgaa gatccctcga ggagcttttt gcaaaagccc
     2341 taggcctcca aaaaagcctc ttcactactt ctggaatagc tcagaggccg aggcggcctc
     2401 ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg
     2461 cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgct
     2521 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     2581 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     2641 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     2701 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     2761 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     2821 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     2881 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     2941 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     3001 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     3061 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     3121 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     3181 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     3241 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     3301 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     3361 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     3421 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     3481 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     3541 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     3601 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     3661 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     3721 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     3781 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     3841 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     3901 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     3961 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     4021 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     4081 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     4141 taccgcgcca catagcagaa ctttaaaagt gctcatcatt gccacctcca cctgaaaacg
     4201 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     4261 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     4321 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     4381 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     4441 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     4501 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     4561 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     4621 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     4681 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     4741 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     4801 aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta
     4861 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     4921 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     4981 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     5041 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     5101 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     5161 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     5221 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg
     5281 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     5341 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     5401 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
     5461 tagggcgaat tcgagctcgc cccgttacat aacttacggt aaatggcccg cctggctgac
     5521 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     5581 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     5641 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     5701 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     5761 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     5821 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     5881 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     5941 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
     6001 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg
     6061 accgatccag cctccggccc ctgggactcc ttcttggtgg cgtgaaactc ccgcacctct
     6121 tcggccagcg ccttgta
//
