LOCUS       V008429                 6083 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008429
VERSION     V008429
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6083)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6083)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6083)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6083)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6083
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..54
                     /label="myr"
                     /note="N-myristoylation signal from Src kinase (Pellman et
                     al., 1985; Kaplan et al., 1988)"
     CDS             61..381
                     /label="FKBP"
                     /note="human FK506-binding protein FKBP12"
     CDS             388..708
                     /codon_start=1
                     /note="unnamed protein product; hFKBP1A"
                     /protein_id="SJL87470.1"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             715..1047
                     /codon_start=1
                     /note="unnamed protein product; hFADD-DED"
                     /protein_id="SJL87471.1"
                     /translation="MDPFLVLLHSVSSSLSSSELTELKFLCLGRVGKRKLERVQSGLDL
                     FSMLLEQNDLEPGHTELLRELLASLRRHDLLRRVDDFEAGAAAGAAPGEEDLCAAFNVI
                     CDNVGKD"
     CDS             1054..1080
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     intron          1112..1684
                     /label="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     CDS             1688..1810
                     /gene="HBB"
                     /label="Hemoglobin subunit beta"
                     /note="Hemoglobin subunit beta from Colobus guereza.
                     Accession#: Q9TT33"
     polyA_signal    1881..1936
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     rep_origin      complement(2289..2424)
                     /direction=LEFT
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     rep_origin      complement(2701..3289)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3460..4332)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL87472.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFQVEVAMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDL
                     VEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTR
                     LDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAE
                     IGASLIKHW"
     promoter        complement(4333..4437)
                     /label="AmpR promoter"
     rep_origin      complement(4769..5224)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5369..5385
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5392..5410
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        5429..5732
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5733..5936
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
ORIGIN
        1 gaattcgcgc gtatggggag tagcaagagc aagcctaagg accccagcca gcgctctaga
       61 ggagtgcagg tggaaaccat ctccccggga gacgggcgca ccttccccaa gcgcggccag
      121 acctgcgtgg tgcactacac cgggatgctt gaagatggaa agaaatttga ttcctcccgg
      181 gacagaaaca agccctttaa gtttatgcta ggcaagcagg aggtgatccg aggctgggaa
      241 gaaggggttg cccagatgag tgtgggtcag agagccaaac tgactatatc tccagattat
      301 gcctatggtg ccactgggca cccaggcatc atcccaccac atgccactct cgtcttcgat
      361 gtggagcttc taaaactgga agtcgagggc gtgcaggtgg aaaccatctc cccaggagac
      421 gggcgcacct tccccaagcg cggccagacc tgcgtggtgc actacaccgg gatgcttgaa
      481 gatggaaaga aatttgattc ctcccgggac agaaacaagc cctttaagtt tatgctaggc
      541 aagcaggagg tgatccgagg ctgggaagaa ggggttgccc agatgagtgt gggtcagaga
      601 gccaaactga ctatatctcc agattatgcc tatggtgcca ctgggcaccc aggcatcatc
      661 ccaccacatg ccactctcgt cttcgatgtg gagcttctaa aactggaaac tagtatggac
      721 ccgttcctgg tgctgctgca ctcggtgtcg tccagcctgt cgagcagcga gctgaccgag
      781 ctcaagttcc tatgcctcgg gcgcgtgggc aagcgcaagc tggagcgcgt gcagagcggc
      841 ctagacctct tctccatgct gctggagcag aacgacctgg agcccgggca caccgagctc
      901 ctgcgcgagc tgctcgcctc cctgcggcgc cacgacctgc tgcggcgcgt cgacgacttc
      961 gaggcggggg cggcggccgg ggccgcgcct ggggaagaag acctgtgtgc agcatttaac
     1021 gtcatatgtg ataatgtggg gaaagattct agttatccgt acgacgtacc agactacgca
     1081 taagaaaagt gaggatcctg agaacttcag ggtgagtttg gggacccttg attgttcttt
     1141 ctttttcgct attgtaaaat tcatgttata tggagggggc aaagttttca gggtgttgtt
     1201 tagaatggga agatgtccct tgtatcacca tggaccctca tgataatttt gtttctttca
     1261 ctttctactc tgttgacaac cattgtctcc tcttattttc ttttcatttt ctgtaacttt
     1321 ttcgttaaac tttagcttgc atttgtaacg aatttttaaa ttcacttttg tttatttgtc
     1381 agattgtaag tactttctct aatcactttt ttttcaaggc aatcagggta tattatattg
     1441 tacttcagca cagttttaga gaacaattgt tataattaaa tgataaggta gaatatttct
     1501 gcatataaat tctggctggc gtggaaatat tcttattggt agaaacaact acatcctggt
     1561 catcatcctg cctttctctt tatggttaca atgatataca ctgtttgaga tgaggataaa
     1621 atactctgag tccaaaccgg gcccctctgc taaccatgtt catgccttct tctttttcct
     1681 acagctcctg ggcaacgtgc tggttgttgt gctgtctcat cattttggca aagaattcac
     1741 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
     1801 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     1861 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     1921 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     1981 tgagtatttg gtttagagtt tggcaacata tgccatatgc tggctgccat gaacaaaggt
     2041 ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata
     2101 gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt
     2161 taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac
     2221 tactcccagt catagctgtc cctcttctct tatgaagatc cctcgaggag ctttttgcaa
     2281 aagccctagg cctccaaaaa agcctcttca ctacttctgg aatagctcag aggccgaggc
     2341 ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga
     2401 actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta
     2461 attgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     2521 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     2581 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     2641 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2701 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     2761 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     2821 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     2881 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     2941 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3001 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3061 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3121 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     3181 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3241 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3301 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3361 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3421 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     3481 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     3541 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     3601 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     3661 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3721 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     3781 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     3841 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     3901 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     3961 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     4021 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     4081 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattgcca cctccacctg
     4141 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     4201 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     4261 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     4321 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     4381 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     4441 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     4501 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     4561 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     4621 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     4681 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     4741 atgcgtaagg agaaaatacc gcatcaggaa attgtaaacg ttaatatttt gttaaaattc
     4801 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     4861 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     4921 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     4981 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     5041 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     5101 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     5161 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     5221 gcgtcgcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     5281 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     5341 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac
     5401 tcactatagg gcgaattcga gctcgccccg ttacataact tacggtaaat ggcccgcctg
     5461 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     5521 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     5581 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     5641 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     5701 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     5761 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     5821 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     5881 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt
     5941 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
     6001 accgggaccg atccagcctc cggcccctgg gactccttct tggtggcgtg aaactcccgc
     6061 acctcttcgg ccagcgcctt gta
//
