LOCUS       62056_6530        3893 bp DNA     circular SYN 20-AUG-2019
DEFINITION  Transformation vector pCMA, complete sequence.
ACCESSION   MN177722
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   LaBrie SD, Dimond ZE, Harrison KS, Baid S, Wickstrum J, Suchland RJ,
            Hefty PS.
  TITLE     Transposon Mutagenesis in Chlamydia trachomatis Identifies CT339 as 
            a ComEC Homolog Important for DNA Uptake and Lateral Gene Transfer
  JOURNAL   MBio 10 (4), e01343-19 (2019)
  PUBMED    31387908
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Dimond ZE, LaBrie SD, Wickstrum J, Hefty PS.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2019) Molecular Biosciences, University of Kansas,
            2034 Haworth Hall 1200 Sunnyside Avenue, Lawrence, KS 66045, USA
REFERENCE   3  (bases 1 to 3893)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio"; 
            date: "2019"; volume: "10"; issue: "4"; pages: "e01343-19"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUL-2019) Molecular Biosciences, University of Kansas, 2034 
            Haworth Hall 1200 Sunnyside Avenue, Lawrence, KS 66045, USA"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Velvet v. 1.2.10
            Assembly Name         :: pCMA5M
            Coverage              :: >50x
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    264..285
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        300..330
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    338..354
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     362..378
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   395..423
                     /rpt_family="Himar repeat"
     CDS             complement(567..1424)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1425..1529)
                     /label=AmpR promoter
     repeat_region   1791..1819
                     /rpt_family="Himar repeat"
     regulatory      1996..2024
                     /label=CT559 promoter
                     /note="CT559 promoter"
                     /regulatory_class="promoter"
     CDS             2046..3092
                     /codon_start=1
                     /transl_table=11
                     /product="Himar C9 transposase"
                     /label=Himar C9 transposase
                     /protein_id="QED88327.1"
                     /translation="MEKKEFRVLIKYCFLKGKNTVEAKTWLDNEFPDSAPGKSTIIDWY
                     AKFKRGEMSTEDGERSGRPKEVVTDENIKKIHKMILNDRKMKLIEIAEALKISKERVGH
                     IIHQYLDMRKLCAKWVPRELTFDQKQRRVDDSKRCLQLLTRNTPEFFRRYVTMDETWLH
                     HYTPESNRQSAEWTATGEPSPKRGKTQKSAGKVMASVFWDAHGIIFIDYLEKGKTINSD
                     YYMALLERLKVEIAAKRPHMKKKKVLFHQDNAPCHKSLRTMAKIHELGFELLPHPPYSP
                     DLAPSDFFLFSDLKRMLAGKKFGCNEEVIAETEAYFEAKPKEYYQNGIKKLEGRYNRCI
                     ALEGNYVE"
     rep_origin      3281..3869
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
       61 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
      121 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
      181 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
      241 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
      301 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
      361 acaggaaaca gctatgacca tgattacgcc aagctaacag gttggatgat aagtccccgg
      421 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
      481 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
      541 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
      601 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
      661 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
      721 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
      781 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
      841 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
      901 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
      961 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     1021 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     1081 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     1141 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     1201 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     1261 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     1321 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     1381 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     1441 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     1501 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     1561 taagcttatc gatgataagc tgtcaaacat gagaattaca acttatatcg tatggggctg
     1621 acttcaggtg ctacatttga agagataaat tgcactgaaa tctagaaata ttttatctga
     1681 ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg ctctgaaaac
     1741 gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa agaccgggga
     1801 cttatcatcc aacctgttac ccgggtaccg agctcgaatt ccggccgccc attcgttcgt
     1861 tctagcttta atgcggattc tgtttttgaa gccatgcgcc agcgggaaac ctcaacctct
     1921 tcgctacttc cgaacaaaga ttagccacat aacctgttca ttttgaagca ttctctgcag
     1981 aaaaaatgtt cccgattggc actaatctcc ccatttgcta tggtgagtga aaaggtgtgc
     2041 ggccgatgga aaaaaaggaa tttcgtgttt tgataaaata ctgttttctg aagggaaaaa
     2101 atacagtgga agcaaaaact tggcttgata atgagtttcc ggactctgcc ccagggaaat
     2161 caacaataat tgattggtat gcaaaattca agcgtggtga aatgagcacg gaggacggtg
     2221 aacgcagtgg acgcccgaaa gaggtggtta ccgacgaaaa catcaaaaaa atccacaaaa
     2281 tgattttgaa tgaccgtaaa atgaagttga tcgagatagc agaggcctta aagatatcaa
     2341 aggaacgtgt tggtcatatc attcatcaat atttggatat gcggaagctc tgtgcgaaat
     2401 gggtgccgcg cgagctcaca tttgaccaaa aacaacgacg tgttgatgat tctaagcggt
     2461 gtttgcagct gttaactcgt aatacacccg agtttttccg tcgatatgtg acaatggatg
     2521 aaacatggct ccatcactac actcctgagt ccaatcgaca gtcggctgag tggacagcga
     2581 ccggtgaacc gtctccgaag cgtggaaaga ctcaaaagtc cgctggcaaa gtaatggcct
     2641 ctgttttttg ggatgcgcat ggaataattt ttatcgatta tcttgagaag ggaaaaacca
     2701 tcaacagtga ctattatatg gcgttattgg agcgtttgaa ggtcgaaatc gcggcaaaac
     2761 ggccccacat gaagaagaaa aaagtgttgt tccaccaaga caacgcaccg tgccacaagt
     2821 cattgagaac gatggcaaaa attcatgaat tgggcttcga attgcttccc cacccgccgt
     2881 attctccaga tctggccccc agcgactttt tcttgttctc agacctcaaa aggatgctcg
     2941 cagggaaaaa atttggctgc aatgaagagg tgatcgccga aactgaggcc tattttgagg
     3001 caaaaccgaa ggagtactac caaaatggta tcaaaaaatt ggaaggtcgt tataatcgtt
     3061 gtatcgctct tgaagggaac tatgttgaat aataagacgt cagccatgga ctgtcagacc
     3121 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3181 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3241 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3301 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3361 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3421 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3481 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3541 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3601 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3661 tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3721 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3781 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3841 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt tta
//
