LOCUS       V008436                10125 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008436
VERSION     V008436
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10125)
  AUTHORS   Chou H-H., Berthet J, Marx CJ.
  TITLE     Growth rate modulates the selective advantage of recurrent
            transposition mutations arising from experimental populations grown
            in a metal-limited environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10125)
  AUTHORS   Marx CJ, Chou H-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2008) Organismic and Evolutionary Biology, Harvard
            University, 16 Divinity Avenue, Biolab 3079, Cambridge, MA 02138,
            USA
REFERENCE   3  (bases 1 to 10125)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10125)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-OCT-2008) Organismic and Evolutionary Biology, Harvard
            University, 16 Divinity Avenue, Biolab 3079, Cambridge, MA 02138,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..712
                     /label="oriV"
                     /note="incP origin of replication"
     rep_origin      1105..1693
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    1981..2002
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2017..2047
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2055..2071
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2079..2095
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      2206..2306
                     /label="PmxaF"
                     /note="PmxaF"
                     /regulatory_class="promoter"
     primer_bind     2446..2462
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(2475..2531)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     CDS             2622..3746
                     /gene="flhA"
                     /label="S-(hydroxymethyl)glutathione dehydrogenase"
                     /note="S-(hydroxymethyl)glutathione dehydrogenase from
                     Paracoccus denitrificans. Accession#: P45382"
     CDS             3792..4631
                     /codon_start=1
                     /gene="fghA"
                     /product="S-formylglutathione hydrolase"
                     /label="fghA"
                     /protein_id="ACR45022.1"
                     /translation="MTLAYETVSENRSFGGIQGVYRHQSQATGTPMTFAIYLPPDARHG
                     KVPVLWYLSGLTCTHENAMTKAGAQEWAAEYGIAVIFPDTSPRGEGVANDETYDLGQGA
                     GFYVDATEAPWAPHFRMWHYVTHELPELVFNNFPLDREAQGITGHSMGGHGALTIAMTF
                     PERYRSVSAFAPIAHPSESDWGRKQFAAYLGDDKAAWKRHDSTILMREKGYPGEVLIDQ
                     GASDQFLDLLKPEALAHAMAERRQPGTFRMQQGYDHSYFFVQSFMADHIRWHAERLG"
     gene            3792..4631
                     /gene="fghA"
                     /label="fghA"
     primer_bind     complement(4683..4699)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(5144..5785)
                     /label="TetR"
                     /note="tetracycline resistance regulatory protein"
     CDS             complement(6566..7378)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(8053..9198)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     CDS             complement(9470..9841)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label="traJ"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(9874..9983)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
ORIGIN
        1 gaccctttcc gacgctcacc gggctggttg ccctcgccgc tgggctggcg gccgtctatg
       61 gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc gagacaccgc ggccgccggc
      121 gttgtggata cctcgcggaa aacttggccc tcactgacag atgaggggcg gacgttgaca
      181 cttgaggggc cgactcaccc ggcgcggcgt tgacagatga ggggcaggct cgatttcggc
      241 cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt
      301 tcccacagat gatgtggaca agcctgggga taagtgccct gcggtattga cacttgaggg
      361 gcgcgactac tgacagatga ggggcgcgat ccttgacact tgaggggcag agtgctgaca
      421 gatgaggggc gcacctattg acatttgagg ggctgtccac aggcagaaaa tccagcattt
      481 gcaagggttt ccgcccgttt ttcggccacc gctaacctgt cttttaacct gcttttaaac
      541 caatatttat aaaccttgtt tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc
      601 cgaagggggg tgccccccct tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc
      661 ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc caaaaatggc agccaagctg
      721 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
      781 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
      841 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
      901 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
      961 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     1021 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1081 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1141 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1201 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     1261 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1321 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1381 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1441 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1501 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1561 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1621 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1681 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1741 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     1801 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     1861 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     1921 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     1981 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     2041 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     2101 ttacgccaag ctagcttccc gcttggtcgg gccgcttcgc gagggcccgt tgacgacaac
     2161 ggtgcgatgg gtcccggccc cggtcaagac gatgccaata cgttgcgaca ctacgccttg
     2221 gcacttttag aattgcctta tcgtcctgat aagaaatgtc cgaccagcta aagacatcgc
     2281 gtccaatcaa agcctagaaa atataggcga agggacgcta ataagtcttt cataagaccg
     2341 cgcaaatcta aaaatatcct tagattcacg atgcggcact tcggatgact tccgagcgag
     2401 cctggaacct cagaaaaacg tctgagagat accgcggatc tcacacagga aacagctatg
     2461 accatgatta cgccaagctt gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg
     2521 agctcgaatt cgcccttgtc tagatgcatg ctcgagcggc cgccagtgtg atggatatct
     2581 gcagaattcg cccttgaggc ctacatgaag gagaagacaa gatgagaacc cgtgccgccg
     2641 tggcccttga ggctggcaaa ccgctggaag tgatggaggt caacctggaa ggcccgaagg
     2701 ccggcgaggt gatggtcgag atcaaggcca ccggcatctg ccacaccgac gaattcaccc
     2761 tgtcgggcgc cgatcccgag gggctcttcc cctcgatcct gggccatgag ggcgcgggcg
     2821 tcgtggtcga ggtcggcccg ggcgttacct cggtcaagcc gggcgatcat gtcatcccgc
     2881 tctacacgcc cgagtgccgg caatgcgcgt cctgcctgtc gggcaagacc aacctttgca
     2941 ccgccattcg cgccacccag ggtcagggcc tgatgccgga cggaaccagc cggttcagca
     3001 tgctcgacgg cacgccgatc tttcattaca tgggctgctc gaccttctcg aactacaccg
     3061 tgctgccgga aatcgcggtg gccaaggtgc gcgaggacgc gcccttcgac aagatctgct
     3121 atatcggctg cggcgtcact accggcatcg gcgcggtgat caacaccgcc aaggtggaaa
     3181 tcggcgccaa ggcggtggtc ttcggcctgg gcggcattgg gctgaacgtg ctgcaggggc
     3241 tgcgcctggc cggcgcggac atgatcatcg gcgtcgatct gaacgacgac aagaagccga
     3301 tggccgagca tttcggcatg acgcatttca tcaacccgaa gaattgcgag aacgtggtgc
     3361 aggagatcgt gaacctgacc aagacgccct tcgaccagat cggcggcgcg gattacagct
     3421 tcgactgcac cggcaacgtc aaggtgatgc gcgacgcgct ggaatgcacc catcgcggct
     3481 ggggccagtc gatcatcatc ggcgtggcgc cggcgggggc cgagatcagc acccgcccgt
     3541 tccagctggt caccggccgg gtctggaagg gcacggcctt cggcggtgcc cgcggccgca
     3601 ccgacgtgcc gcagatcgtc gactggtata tggacggcaa gatcgagatc gacccgatga
     3661 tcacccacac cctgtcgctg gacgacatca acaagggctt cgacctgatg catgcgggcg
     3721 aatccatccg ctcggtcgtc ctttactgat cgtcaagggc tgcttatcta gagttccacg
     3781 acttgacaga catgacgctg gcctatgaaa ccgtatcgga aaaccgcagc ttcggcggta
     3841 tccagggcgt ctatcgccac cagtcccagg cgaccggcac gccgatgacc tttgccatct
     3901 acctgccgcc cgatgcccgg cacggcaagg tgccggtgct gtggtatctt tcgggcctga
     3961 cctgcacgca tgaaaatgcc atgaccaagg ccggcgcaca ggaatgggcg gcggaatacg
     4021 gcatcgcggt gatctttccc gacacctcgc cgcgcggcga aggcgtggcc aacgacgaga
     4081 cctacgacct gggccagggc gccggcttct atgtcgatgc gaccgaggcg ccctgggcgc
     4141 cgcatttcag gatgtggcac tatgtcaccc atgaactgcc cgagctggtc ttcaacaact
     4201 tcccgctgga ccgcgaggcg cagggcatca ccggccattc catgggcggg cacggtgcgc
     4261 tgaccatcgc catgaccttc ccggagcgct atcgctcggt ctcggccttt gcgccgatcg
     4321 cgcatcccag cgaatccgac tgggggcgca aacagttcgc cgcctatctg ggcgacgaca
     4381 aggcggcatg gaaaaggcat gattcgacca tcctgatgcg cgaaaagggc tatcccggcg
     4441 aggtgctgat cgaccagggc gcgagcgacc agttcctcga tcttctgaag cccgaggcgc
     4501 tggcccatgc catggccgaa cgccgccagc ccggcacctt ccgcatgcag cagggctacg
     4561 atcacagcta tttcttcgtg caaagcttca tggccgacca tatccgctgg cacgcggagc
     4621 ggctgggcta ggcggccggg accggccaaa gggcaacggg gcagagctca aagggcgaat
     4681 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     4741 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     4801 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
     4861 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
     4921 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     4981 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     5041 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     5101 ctatttttat aggttaatgt catgataata atggtttctt agcacccttt ctcggtcctt
     5161 caacgttcct gacaacgagc ctccttttcg ccaatccatc gacaatcacc gcgagtccct
     5221 gctcgaacgc tgcgtccgga ccggcttcgt cgaaggcgtc tatcgcggcc cgcaacagcg
     5281 gcgagagcgg agcctgttca acggtgccgc cgcgctcgcc ggcatcgctg tcgccggcct
     5341 gctcctcaag cacggcccca acagtgaagt agctgattgt catcagcgca ttgacggcgt
     5401 ccccggccga aaaacccgcc tcgcagagga agcgaagctg cgcgtcggcc gtttccatct
     5461 gcggtgcgcc cggtcgcgtg ccggcatgga tgcgcgcgcc atcgcggtag gcgagcagcg
     5521 cctgcctgaa gctgcgggca ttcccgatca gaaatgagcg ccagtcgtcg tcggctctcg
     5581 gcaccgaatg cgtatgattc tccgccagca tggcttcggc cagtgcgtcg agcagcgccc
     5641 gcttgttcct gaagtgccag taaagcgccg gctgctgaac ccccaaccgt tccgccagtt
     5701 tgcgtgtcgt cagaccgtct acgccgacct cgttcaacag gtccagggcg gcacggatca
     5761 ctgtattcgg ctgcaacttt gtcatgcttg acactttatc actgataaac ataatatgtc
     5821 caccaactta tcagtgataa agaatccgcg cgttcaatcg gaccagcgga ggctggtccg
     5881 gaggccagac gtgaaaccca acatacccct gatcgtaatt ctgagcactg tcgcgctcga
     5941 cgctgtcggc atcggcctga ttatgccggt gctgccgggc ctcctgcgcg atctggttca
     6001 ctcgaacgac gtcaccgccc actatggcat tctgctggcg ctgtatgcgt tggtgcaatt
     6061 tgcctgcgca cctgtgctgg gcgcgctgtc ggatcgtttc gggcggcggc caatcttgct
     6121 cgtctcgctg gccggcgcca ctgtcgacta cgccatcatg gcgacagcgc ctttcctttg
     6181 ggttctctat atcgggcgga tcgtggccgg catcaccggg gcgactgggg cggtagccgg
     6241 cgcttatatt gccgatgacc tgcagggggg ggggggcgct gaggtctgcc tcgtgaagaa
     6301 ggtgttgctg actcatacca ggcctgaatc gccccatcat ccagccagaa agtgagggag
     6361 ccacggttga tgagagcttt gttgtaggtg gaccagttgg tgattttgaa cttttgcttt
     6421 gccacggaac ggtctgcgtt gtcgggaaga tgcgtgatct gatccttcaa ctcagcaaaa
     6481 gttcgattta ttcaacaaag ccgccgtccc gtcaagtcag cgtaatgctc tgccagtgtt
     6541 acaaccaatt aaccaattct gattagaaaa actcatcgag catcaaatga aactgcaatt
     6601 tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag
     6661 aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga
     6721 ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg
     6781 agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagctta tgcatttctt
     6841 tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca
     6901 aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag
     6961 gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa
     7021 tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc ccggggatcg
     7081 cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag
     7141 gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc
     7201 tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga
     7261 ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat
     7321 ccatgttgga atttaatcgc ggcctcgagc aagacgtttc ccgttgaata tggctcataa
     7381 caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt
     7441 tatcttgtgc aatgtaacat cagagatttt gagacacaac gtggctttcc cccccccccc
     7501 tgcaggtccg acacggggat ggatggcgtt cccgatcatg gtcctgcttg cttcgggtgg
     7561 catcggaatg ccggcgctgc aagcaatgtt gtccaggcag gtggatgagg aacgtcaggg
     7621 gcagctgcaa ggctcactgg cggcgctcac cagcctgacc tcgatcgtcg gacccctcct
     7681 cttcacggcg atctatgcgg cttctataac aacgtggaac gggtgggcat ggattgcagg
     7741 cgctgccctc tacttgctct gcctgccggc gctgcgtcgc gggctttgga gcggcgcagg
     7801 gcaacgagcc gatcgctgat cgtggaaacg ataggcctat gccatgcggg tcaaggcgac
     7861 ttccggcaag ctatacgcgc cctagaattg tcaattttaa tcctctgttt atcggcagtt
     7921 cgtagagcgc gccgtgcgtc ccgagcgata ctgagcgaag caagtgcgtc gagcagtgcc
     7981 cgcttgttcc tgaaatgcca gtaaagcgct ggctgctgaa cccccagccg gaactgaccc
     8041 cacaaggccc tagcgtttgc aatgcaccag gtcatcattg acccaggcgt gttccaccag
     8101 gccgctgcct cgcaactctt cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg
     8161 ggtggaatcc gatccgcaca tgaggcggaa ggtttccagc ttgagcgggt acggctcccg
     8221 gtgcgagctg aaatagtcga acatccgtcg ggccgtcggc gacagcttgc ggtacttctc
     8281 ccatatgaat ttcgtgtagt ggtcgccagc aaacagcacg acgatttcct cgtcgatcag
     8341 gacctggcaa cgggacgttt tcttgccacg gtccaggacg cggaagcggt gcagcagcga
     8401 caccgattcc aggtgcccaa cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg
     8461 cgacaggcat tcctcggcct tcgtgtaata ccggccattg atcgaccagc ccaggtcctg
     8521 gcaaagctcg tagaacgtga aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc
     8581 caacacctgc tgccacacca gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt
     8641 gatcttcacg tccttgttga cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat
     8701 tttcttgttg cgcgtggtga acagggcaga gcgggccgtg tcgtttggca tcgctcgcat
     8761 cgtgtccggc cacggcgcaa tatcgaacaa ggaaagctgc atttccttga tctgctgctt
     8821 cgtgtgtttc agcaacgcgg cctgcttggc ctcgctgacc tgttttgcca ggtcctcgcc
     8881 ggcggttttt cgcttcttgg tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc
     8941 caaacctgcc gcctcctgtt cgagacgacg cgaacgctcc acggcggccg atggcgcggg
     9001 cagggcaggg ggagccagtt gcacgctgtc gcgctcgatc ttggccgtag cttgctggac
     9061 catcgagccg acggactgga aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat
     9121 ggtttcggca tcctcggcgg aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg
     9181 gtcaaacgtc cgattcattc accctccttg cgggattgcc ccgactcacg ccggggcaat
     9241 gtgcccttat tcctgatttg acccgcctgg tgccttggtg tccagataat ccaccttatc
     9301 ggcaatgaag tcggtcccgt agaccgtctg gccgtccttc tcgtacttgg tattccgaat
     9361 cttgccctgc acgaatacca gctccgcgaa gtcgctcttc ttgatggagc gcatggggac
     9421 gtgcttggca atcacgcgca ccccccggcc gttttagcgg ctaaaaaagt catggctctg
     9481 ccctcgggcg gaccacgccc atcatgacct tgccaagctc gtcctgcttc tcttcgatct
     9541 tcgccagcag ggcgaggatc gtggcatcac cgaaccgcgc cgtgcgcggg tcgtcggtga
     9601 gccagagttt cagcaggccg cccaggcggc ccaggtcgcc attgatgcgg gccagctcgc
     9661 ggacgtgctc atagtccacg acgcccgtga ttttgtagcc ctggccgacg gccagcaggt
     9721 aggcctacag gctcatgccg gccgccgccg ccttttcctc aatcgctctt cgttcgtctg
     9781 gaaggcagta caccttgata ggtgggctgc ccttcctggt tggcttggtt tcatcagcca
     9841 tccgcttgcc ctcatctgtt acgccggcgg tagccggcca gcctcgcaga gcaggattcc
     9901 cgttgagcac cgccaggtgc gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg
     9961 ggcctacttc acctatcctg cccggctgac gccgttggat acaccaagga aagtctacac
    10021 gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga tggatatacc gaaaaaatcg
    10081 ctataatgac cccgaagcag ggttatgcag cggaaaagat ccgtc
//
