LOCUS       40924_11326       11299 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCM132, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11299)
  AUTHORS   Marx CJ, Lidstrom ME.
  TITLE     Development of improved versatile broad-host-range vectors for use 
            in methylotrophs and other Gram-negative bacteria
  JOURNAL   Microbiology 147 (Pt 8), 2065-2075 (2001)
  PUBMED    11495985
REFERENCE   2  (bases 1 to 11299)
  AUTHORS   Marx CJ, Lidstrom ME.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2000) Microbiology, University of Washington, Box 
            357242, Seattle, WA 98195, USA
REFERENCE   3  (bases 1 to 11299)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11299)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology 147 (Pt 8), 2065-2075 (2001)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-DEC-2000) Microbiology, University of Washington, Box 357242, 
            Seattle, WA 98195, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..712
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      1105..1693
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1981..2002
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2017..2047
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2055..2071
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2079..2095
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2235..5267)
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="RVDRENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGE
                     WRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPT
                     ENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSA
                     FLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDD
                     FSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRL
                     NVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIR
                     GVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVD
                     EANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDA
                     LYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGE
                     TRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAY
                     GGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHS
                     DNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNAT
                     AWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFL
                     SQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQ
                     CTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIG
                     LNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTR
                     ELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSP
                     SVSAELQLSAGRYHYQLVWCQK"
     terminator      complement(5470..5497)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(5589..5675)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     complement(5857..5873)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(6318..6959)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKG"
     CDS             complement(7740..8552)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9227..10372)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(10644..11015)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     CDS             complement(10899..11015)
                     /codon_start=1
                     /gene="traJ'"
                     /product="TraJ'"
                     /label=traJ'
                     /note="truncated oriT-recognizing protein"
                     /protein_id="AAK73426.1"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL"
     gene            complement(10899..11015)
                     /gene="traJ'"
                     /label=traJ'
     oriT            complement(11048..11157)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
ORIGIN
        1 gaccctttcc gacgctcacc gggctggttg ccctcgccgc tgggctggcg gccgtctatg
       61 gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc gagacaccgc ggccgccggc
      121 gttgtggata cctcgcggaa aacttggccc tcactgacag atgaggggcg gacgttgaca
      181 cttgaggggc cgactcaccc ggcgcggcgt tgacagatga ggggcaggct cgatttcggc
      241 cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt
      301 tcccacagat gatgtggaca agcctgggga taagtgccct gcggtattga cacttgaggg
      361 gcgcgactac tgacagatga ggggcgcgat ccttgacact tgaggggcag agtgctgaca
      421 gatgaggggc gcacctattg acatttgagg ggctgtccac aggcagaaaa tccagcattt
      481 gcaagggttt ccgcccgttt ttcggccacc gctaacctgt cttttaacct gcttttaaac
      541 caatatttat aaaccttgtt tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc
      601 cgaagggggg tgccccccct tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc
      661 ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc caaaaatggc agccaagctg
      721 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
      781 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
      841 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
      901 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
      961 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     1021 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1081 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1141 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1201 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     1261 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1321 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1381 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1441 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1501 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1561 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1621 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1681 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1741 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     1801 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     1861 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     1921 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     1981 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     2041 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     2101 ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tcaattcggc ttccaccgga
     2161 attagcttga aatagtacat aatggatttc cttacgcgaa atacgggcag acatggcctg
     2221 cccggttatt attatttttg acaccagacc aactggtaat ggtagcgacc ggcgctcagc
     2281 tgtaattccg ccgatactga cgggctccag gagtcgtcgc caccaatccc catatggaaa
     2341 ccgtcgatat tcagccatgt gccttcttcc gcgtgcagca gatggcgatg gctggtttcc
     2401 atcagttgct gttgactgta gcggctgatg ttgaactgga agtcgccgcg ccactggtgt
     2461 gggccataat tcaattcgcg cgtcccgcag cgcagaccgt tttcgctcgg gaagacgtac
     2521 ggggtataca tgtctgacaa tggcagatcc cagcggtcaa aacaggcggc agtaaggcgg
     2581 tcgggatagt tttcttgcgg ccctaatccg agccagttta cccgctctgc tacctgcgcc
     2641 agctggcagt tcaggccaat ccgcgccgga tgcggtgtat cgctcgccac ttcaacatca
     2701 acggtaatcg ccatttgacc actaccatca atccggtagg ttttccggct gataaataag
     2761 gttttcccct gatgctgcca cgcgtgagcg gtcgtaatca gcaccgcatc agcaagtgta
     2821 tctgccgtgc actgcaacaa cgctgcttcg gcctggtaat ggcccgccgc cttccagcgt
     2881 tcgacccagg cgttagggtc aatgcgggtc gcttcactta cgccaatgtc gttatccagc
     2941 ggtgcacggg tgaactgatc gcgcagcggc gtcagcagtt gttttttatc gccaatccac
     3001 atctgtgaaa gaaagcctga ctggcggtta aattgccaac gcttattacc cagctcgatg
     3061 caaaaatcca tttcgctggt ggtcagatgc gggatggcgt gggacgcggc ggggagcgtc
     3121 acactgaggt tttccgccag acgccactgc tgccaggcgc tgatgtgccc ggcttctgac
     3181 catgcggtcg cgttcggttg cactacgcgt actgtgagcc agagttgccc ggcgctctcc
     3241 ggctgcggta gttcaggcag ttcaatcaac tgtttacctt gtggagcgac atccagaggc
     3301 acttcaccgc ttgccagcgg cttaccatcc agcgccacca tccagtgcag gagctcgtta
     3361 tcgctatgac ggaacaggta ttcgctggtc acttcgatgg tttgcccgga taaacggaac
     3421 tggaaaaact gctgctggtg ttttgcttcc gtcagcgctg gatgcggcgt gcggtcggca
     3481 aagaccagac cgttcataca gaactggcga tcgttcggcg tatcgccaaa atcaccgccg
     3541 taagccgacc acgggttgcc gttttcatca tatttaatca gcgactgatc cacccagtcc
     3601 cagacgaagc cgccctgtaa acggggatac tgacgaaacg cctgccagta tttagcgaaa
     3661 ccgccaagac tgttacccat cgcgtgggcg tattcgcaaa ggatcagcgg gcgcgtctct
     3721 ccaggtagcg aaagccattt tttgatggac catttcggca cagccgggaa gggctggtct
     3781 tcatccacgc gcgcgtacat cgggcaaata atatcggtgg ccgtggtgtc ggctccgccg
     3841 ccttcatact gcaccgggcg ggaaggatcg acagatttga tccagcgata cagcgcgtcg
     3901 tgattagcgc cgtggcctga ttcattcccc agcgaccaga tgatcacact cgggtgatta
     3961 cgatcgcgct gcaccattcg cgttacgcgt tcgctcatcg ccggtagcca gcgcggatca
     4021 tcggtcagac gattcattgg caccatgccg tgggtttcaa tattggcttc atccaccaca
     4081 tacaggccgt agcggtcgca cagcgtgtac cacagcggat ggttcggata atgcgaacag
     4141 cgcacggcgt taaagttgtt ctgcttcatc agcaggatat cctgcaccat cgtctgctca
     4201 tccatgacct gaccatgcag aggatgatgc tcgtgacggt taacgcctcg aatcagcaac
     4261 ggcttgccgt tcagcagcag cagaccattt tcaatccgca cctcgcggaa accgacatcg
     4321 caggcttctg cttcaatcag cgtgccgtcg gcggtgtgca gttcaaccac cgcacgatag
     4381 agattcggga tttcggcgct ccacagtttc gggttttcga cgttcagacg tagtgtgacg
     4441 cgatcggcat aaccaccacg ctcatcgata atttcaccgc cgaaaggcgc ggtgccgctg
     4501 gcgacctgcg tttcaccctg ccataaagaa actgttaccc gtaggtagtc acgcaactcg
     4561 ccgcacatct gaacttcagc ctccagtaca gcgcggctga aatcatcatt aaagcgagtg
     4621 gcaacatgga aatcgctgat ttgtgtagtc ggtttatgca gcaacgagac gtcacggaaa
     4681 atgccgctca tccgccacat atcctgatct tccagataac tgccgtcact ccaacgcagc
     4741 accatcaccg cgaggcggtt ttctccggcg cgtaaaaatg cgctcaggtc aaattcagac
     4801 ggcaaacgac tgtcctggcc gtaaccgacc cagcgcccgt tgcaccacag atgaaacgcc
     4861 gagttaacgc catcaaaaat aattcgcgtc tggccttcct gtagccagct ttcatcaaca
     4921 ttaaatgtga gcgagtaaca acccgtcgga ttctccgtgg gaacaaacgg cggattgacc
     4981 gtaatgggat aggttacgtt ggtgtagatg ggcgcatcgt aaccgtgcat ctgccagttt
     5041 gaggggacga cgacagtatc ggcctcagga agatcgcact ccagccagct ttccggcacc
     5101 gcttctggtg ccggaaacca ggcaaagcgc cattcgccat tcaggctgcg caactgttgg
     5161 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     5221 gcaaggcgat taagttgggt aacgccaggg ttttcccggt cgacccgtaa tcttacgtca
     5281 gtaacttcca cagtagttca ccaccttttc cctatagatc ttccgtgcag tttaagccga
     5341 attgatcccc gggtaccgag ctcgaatcta gaattccctg ctttcctgat gcaaaaacga
     5401 ggctagttta ccgtatctgt ggggggatgg cttgtagata tgacgacagg aagagtttgt
     5461 agaaacgcaa aaaggccatc cgtcaggatg gccttctgct taatttgatg cctggcagtt
     5521 tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcgcaacgtt caaatccgct
     5581 cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag
     5641 gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc
     5701 atggggagac cccacactac catcggcgct acggcgtttc acttctgagt tcggcatggg
     5761 gtcaggtggg accaccgcgc tactgccgcc aggcaaattc tgttttatca gaccgcttct
     5821 gcgttctgat ttaatctgta tcaggctgaa aaattcactg gccgtcgttt tacaacgtcg
     5881 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     5941 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     6001 gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     6061 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     6121 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     6181 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     6241 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     6301 aataatggtt tcttagcacc ctttctcggt ccttcaacgt tcctgacaac gagcctcctt
     6361 ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc cggaccggct
     6421 tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg ttcaacggtg
     6481 ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc cccaacagtg
     6541 aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc cgcctcgcag
     6601 aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg cgtgccggca
     6661 tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg ggcattcccg
     6721 atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg attctccgcc
     6781 agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg ccagtaaagc
     6841 gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc gtctacgccg
     6901 acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa ctttgtcatg
     6961 attgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg ataaagaatc
     7021 cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa cccaacatac
     7081 ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc ctgattatgc
     7141 cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc gcccactatg
     7201 gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg ctgggcgcgc
     7261 tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc gccactgtcg
     7321 actacgccat catggcgaca gcgcctttcc tttgggttct ctatatcggg cggatcgtgg
     7381 ccggcatcac cggggcgact ggggcggtag ccggcgctta tattgccgat gacctgcagg
     7441 gggggggggg cgctgaggtc tgcctcgtga agaaggtgtt gctgactcat accaggcctg
     7501 aatcgcccca tcatccagcc agaaagtgag ggagccacgg ttgatgagag ctttgttgta
     7561 ggtggaccag ttggtgattt tgaacttttg ctttgccacg gaacggtctg cgttgtcggg
     7621 aagatgcgtg atctgatcct tcaactcagc aaaagttcga tttattcaac aaagccgccg
     7681 tcccgtcaag tcagcgtaat gctctgccag tgttacaacc aattaaccaa ttctgattag
     7741 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
     7801 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
     7861 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
     7921 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
     7981 tccggtgaga atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca
     8041 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
     8101 tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc
     8161 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct
     8221 tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca
     8281 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt
     8341 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
     8401 tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta
     8461 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctc
     8521 gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa
     8581 gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta acatcagaga
     8641 ttttgagaca caacgtggct ttcccccccc cccctgcagg tccgacacgg ggatggatgg
     8701 cgttcccgat catggtcctg cttgcttcgg gtggcatcgg aatgccggcg ctgcaagcaa
     8761 tgttgtccag gcaggtggat gaggaacgtc aggggcagct gcaaggctca ctggcggcgc
     8821 tcaccagcct gacctcgatc gtcggacccc tcctcttcac ggcgatctat gcggcttcta
     8881 taacaacgtg gaacgggtgg gcatggattg caggcgctgc cctctacttg ctctgcctgc
     8941 cggcgctgcg tcgcgggctt tggagcggcg cagggcaacg agccgatcgc tgatcgtgga
     9001 aacgataggc ctatgccatg cgggtcaagg cgacttccgg caagctatac gcgccctaga
     9061 attgtcaatt ttaatcctct gtttatcggc agttcgtaga gcgcgccgtg cgtcccgagc
     9121 gatactgagc gaagcaagtg cgtcgagcag tgcccgcttg ttcctgaaat gccagtaaag
     9181 cgctggctgc tgaaccccca gccggaactg accccacaag gccctagcgt ttgcaatgca
     9241 ccaggtcatc attgacccag gcgtgttcca ccaggccgct gcctcgcaac tcttcgcagg
     9301 cttcgccgac ctgctcgcgc cacttcttca cgcgggtgga atccgatccg cacatgaggc
     9361 ggaaggtttc cagcttgagc gggtacggct cccggtgcga gctgaaatag tcgaacatcc
     9421 gtcgggccgt cggcgacagc ttgcggtact tctcccatat gaatttcgtg tagtggtcgc
     9481 cagcaaacag cacgacgatt tcctcgtcga tcaggacctg gcaacgggac gttttcttgc
     9541 cacggtccag gacgcggaag cggtgcagca gcgacaccga ttccaggtgc ccaacgcggt
     9601 cggacgtgaa gcccatcgcc gtcgcctgta ggcgcgacag gcattcctcg gccttcgtgt
     9661 aataccggcc attgatcgac cagcccaggt cctggcaaag ctcgtagaac gtgaaggtga
     9721 tcggctcgcc gataggggtg cgcttcgcgt actccaacac ctgctgccac accagttcgt
     9781 catcgtcggc ccgcagctcg acgccggtgt aggtgatctt cacgtccttg ttgacgtgga
     9841 aaatgacctt gttttgcagc gcctcgcgcg ggattttctt gttgcgcgtg gtgaacaggg
     9901 cagagcgggc cgtgtcgttt ggcatcgctc gcatcgtgtc cggccacggc gcaatatcga
     9961 acaaggaaag ctgcatttcc ttgatctgct gcttcgtgtg tttcagcaac gcggcctgct
    10021 tggcctcgct gacctgtttt gccaggtcct cgccggcggt ttttcgcttc ttggtcgtca
    10081 tagttcctcg cgtgtcgatg gtcatcgact tcgccaaacc tgccgcctcc tgttcgagac
    10141 gacgcgaacg ctccacggcg gccgatggcg cgggcagggc agggggagcc agttgcacgc
    10201 tgtcgcgctc gatcttggcc gtagcttgct ggaccatcga gccgacggac tggaaggttt
    10261 cgcggggcgc acgcatgacg gtgcggcttg cgatggtttc ggcatcctcg gcggaaaacc
    10321 ccgcgtcgat cagttcttgc ctgtatgcct tccggtcaaa cgtccgattc attcaccctc
    10381 cttgcgggat tgccccgact cacgccgggg caatgtgccc ttattcctga tttgacccgc
    10441 ctggtgcctt ggtgtccaga taatccacct tatcggcaat gaagtcggtc ccgtagaccg
    10501 tctggccgtc cttctcgtac ttggtattcc gaatcttgcc ctgcacgaat accagctccg
    10561 cgaagtcgct cttcttgatg gagcgcatgg ggacgtgctt ggcaatcacg cgcacccccc
    10621 ggccgtttta gcggctaaaa aagtcatggc tctgccctcg ggcggaccac gcccatcatg
    10681 accttgccaa gctcgtcctg cttctcttcg atcttcgcca gcagggcgag gatcgtggca
    10741 tcaccgaacc gcgccgtgcg cgggtcgtcg gtgagccaga gtttcagcag gccgcccagg
    10801 cggcccaggt cgccattgat gcgggccagc tcgcggacgt gctcatagtc cacgacgccc
    10861 gtgattttgt agccctggcc gacggccagc aggtaggcct acaggctcat gccggccgcc
    10921 gccgcctttt cctcaatcgc tcttcgttcg tctggaaggc agtacacctt gataggtggg
    10981 ctgcccttcc tggttggctt ggtttcatca gccatccgct tgccctcatc tgttacgccg
    11041 gcggtagccg gccagcctcg cagagcagga ttcccgttga gcaccgccag gtgcgaataa
    11101 gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat cctgcccggc
    11161 tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa tcctgtatat
    11221 cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa gcagggttat
    11281 gcagcggaaa agatccgtc
//
