LOCUS       pCLuc_Mini-TK_2.        6131 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector with a minimal promoter for measuring promoter or 
            enhancer activity using secreted Cypridina luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pCLuc Mini-TK 2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6131)
  AUTHORS   New England Biolabs
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6131)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    20..68
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        69..131
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     CDS             145..1803
                     /label=CLuc
                     /note="secreted Cypridina luciferase"
     polyA_signal    1815..1863
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      1997..2425
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2439..2769
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2836..3627
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3804..3937
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3974..3990)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3998..4014)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4022..4052)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4067..4088)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4376..4964)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5138..5995)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5996..6100)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatcttgg aattctgcag atatcctcga gcccaagctt ggtaccgagc
       61 tcggatcctt cgcatattaa ggtgacgcgt gtggcctcga acaccgagcg accctgcagc
      121 gacccgctta aaagatccgc caccatgaag accttaattc ttgccgttgc attagtctac
      181 tgcgccactg ttcattgcca ggactgtcct tacgaacctg atccaccaaa cacagttcca
      241 acttcctgtg aagctaaaga aggagaatgt attgatagca gctgtggcac ctgcacgaga
      301 gacatactat cagatggact gtgtgaaaat aaaccaggaa aaacatgttg ccgaatgtgt
      361 cagtatgtaa ttgaatgcag agtagaggcc gcaggatggt ttagaacatt ctatggaaag
      421 agattccagt tccaggaacc tggtacatac gtgttgggtc aaggaaccaa gggcggcgac
      481 tggaaggtgt ccatcaccct ggagaacctg gatggaacca agggggctgt gctgaccaag
      541 acaagactgg aagtggctgg agacatcatt gacatcgctc aagctactga gaatcccatc
      601 actgtaaacg gtggagctga ccctatcatc gccaacccgt acaccatcgg cgaggtcacc
      661 atcgctgttg ttgagatgcc aggcttcaac atcaccgtca ttgagttctt caaactgatc
      721 gtgatcgaca tcctcggagg aagatctgta agaatcgccc cagacacagc aaacaaagga
      781 atgatctctg gcctctgtgg agatcttaaa atgatggaag atacagactt cacttcagat
      841 ccagaacaac tcgctattca gcctaagatc aaccaggagt ttgacggttg tccactctat
      901 ggaaatcctg atgacgttgc atactgcaaa ggtcttctgg agccgtacaa ggacagctgc
      961 cgcaacccca tcaacttcta ctactacacc atctcctgcg ccttcgcccg ctgtatgggt
     1021 ggagacgagc gagcctcaca cgtgctgctt gactacaggg agacgtgcgc tgctcccgaa
     1081 actagaggaa cctgcgtttt gtctggacat actttctacg atacatttga caaagcaaga
     1141 taccaattcc agggtccctg caaggagatt cttatggccg ccgactgttt ctggaacact
     1201 tgggatgtga aggtttcaca caggaatgtt gactcttaca ctgaagtaga gaaagtacga
     1261 atcaggaaac aatcgactgt agtagaactc attgttgatg gaaaacagat tctggttgga
     1321 ggagaagccg tgtccgtccc gtacagctct cagaacactt ccatctactg gcaagatggt
     1381 gacatactga ctacagccat cctacctgaa gctctggtgg tcaagttcaa cttcaagcaa
     1441 ctgctcgtcg tacatattag agatccattc gatggtaaga cttgcggtat ttgcggtaac
     1501 tacaaccagg atttcagtga tgattctttt gatgctgaag gagcctgtga tctgaccccc
     1561 aacccaccgg gatgcaccga agaacagaaa cctgaagctg aacgactctg caatagtctc
     1621 ttcgccggtc aaagtgatct tgatcagaaa tgtaacgtgt gccacaagcc tgaccgtgtc
     1681 gaacgatgca tgtacgagta ttgcctgagg ggacaacagg gtttctgtga ccacgcatgg
     1741 gagttcaaga aagaatgcta cataaagcat ggagacaccc tagaagtacc agatgaatgc
     1801 aaataggcgg ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt
     1861 gtgtctagaa ataattctta ctgtcatgcc aagtaagatg cttttctgtg ctgcaatagc
     1921 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     1981 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     2041 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     2101 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg gggcatccct
     2161 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     2221 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     2281 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     2341 tattcttttg atttataagg gattttgggg atttcggcct attggttaaa aaatgagctg
     2401 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     2461 agtccccagg ctccccaggc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     2521 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     2581 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     2641 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     2701 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     2761 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca agagacagga
     2821 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     2881 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     2941 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     3001 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     3061 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     3121 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     3181 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     3241 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     3301 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     3361 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     3421 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     3481 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     3541 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     3601 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     3661 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     3721 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     3781 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     3841 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     3901 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     3961 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     4021 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     4081 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     4141 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     4201 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     4261 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     4321 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     4381 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     4441 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     4501 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     4561 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     4621 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     4681 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     4741 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     4801 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4861 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     4921 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     4981 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     5041 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     5101 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     5161 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     5221 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     5281 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     5341 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     5401 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     5461 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     5521 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     5581 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     5641 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     5701 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     5761 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     5821 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     5881 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     5941 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     6001 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     6061 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     6121 cacctgacgt c
//
