LOCUS       V012266                13362 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V012266
VERSION     V012266
KEYWORDS    pCLIP-All-hCMV-ZsGreen
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13362)
  AUTHORS   transOMIC
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 13362)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..404
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             411..668
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    715..840
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1333..1566
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1751..1795
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1944..1985
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2093..2210
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2261..2501
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    2506..4390
                     /label="Stuffer"
                     /note="Stuffer"
     misc_RNA        4391..4466
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     terminator      4467..4472
                     /note="polIII terminator"
                     /note="RNA polymerase III transcription terminator"
     enhancer        4479..4858
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4859..5062
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             5117..9220
                     /label="Cas9"
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
     CDS             9221..9268
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label="bipartite nuclear localization signal from nucl"
                     /note="nucleoplasmin NLS"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             9269..9292
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             9302..9358
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label="2A peptide from porcine teschovirus-1 polyprotein"
                     /note="P2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9359..10051
                     /label="ZsGreen1"
                     /note="Zoanthus green fluorescent protein"
     misc_feature    10068..10656
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             10864..11097
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        11193..11522
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(11611..12196)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(12370..13227)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(13228..13332)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatcctcg cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accagctagc gaggcgtggc
      421 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
      481 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
      541 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
      601 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
      661 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
      721 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
      781 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
      841 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
      901 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
      961 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     1021 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     1081 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     1141 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     1201 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1261 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1321 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1381 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     1441 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     1501 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     1561 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     1621 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     1681 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     1741 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     1801 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     1861 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     1921 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     1981 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     2041 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     2101 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2161 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     2221 acagggacag cagagatcca gtttggttaa ttaaggtacc gagggcctat ttcccatgat
     2281 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac
     2341 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
     2401 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa
     2461 gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg gagacggttg
     2521 taaatgagca cacaaaatac acatgctaaa atattatatt ctatgacctt tataaaatca
     2581 accaaaatct tctttttaat aactttagta tcaataatta gaatttttat gttccttttt
     2641 gcaaactttt aataaaaatg agcaaaataa aaaaacgcta gttttagtaa ctcgcgttgt
     2701 tttcttcacc tttaataata gctactccac cacttgttcc taagcggtca gctcctgctt
     2761 caatcatttt ttgagcatct tcaaatgttc taactccacc agctgcttta actaaagcat
     2821 tgtctttaac aactgacttc attagtttaa catcttcaaa tgttgcacct gattttgaaa
     2881 atcctgttga tgttttaaca aattctaatc cagcttcaac agctatttca caagctttca
     2941 tgatttcttc ttttgttaat aaacaatttt ccataataca tttaacaaca tgtgatccag
     3001 ctgctttttt tacagctttc atgtcttcta aaactaattc ataatttttg tcttttaatg
     3061 caccaatatt taataccata tcaatttctg ttgcaccatc tttaattgct tcagaaactt
     3121 cgaatgcttt tgtagctgtt gtgcatgcac ctagaggaaa acctacaaca tttgttattc
     3181 ctacatttgt gccttttaat aattctttac aatagcttgt tcaatatgaa ttaacacaaa
     3241 ctgttgcaaa atcaaattca attgcttcat cacataattg tttaatttca gctttcgtag
     3301 catcttgttt taataatgtg tgatctatat atttgtttag tttcattttt tctcctatat
     3361 attcattttt aattttaatt ctttaataat ttcgtctact ttaactttag cgttttgaac
     3421 agattcacca acacctataa aataaatttt tagtttaggt tcagttccac ttgggcgaac
     3481 agcaaatcat gacttatctt ctaaataaaa ttttagtaag tcttgtcctg gcatattata
     3541 cattccatcg atgtagtctt caacattaac aactttaagt ccagcaattt gagttaaggg
     3601 tgttgctctc aatgatttca ttaatggttc aatttttaat ttcttttctt ctggtttaaa
     3661 attcaagttt aaagtgaaag tgtaatatgc acccatttct ttaaataaat cttctaaata
     3721 gtctactaat gttttatttt gttttttata aaatcaagca gcctctgcta ttaatataga
     3781 agcttgtatt ccatctttat ctctagctga gtcatcaatt acatatccat aactttcttc
     3841 ataagcaaaa acaaaattta atccgttatc ttcttcttta gcaatttctc tacccattca
     3901 tttaaatcca gttaaagttt ttacaatatt aactccatat ttttcatgag cgattctatc
     3961 acccaaatca cttgttacaa aacttgaata tagagccgga ttttttggaa tgctatttaa
     4021 gcgttttaga tttgataatt ttcaatcaat taaaattggt cctgtttgat ttccatctaa
     4081 tcttacaaaa tgaccatcat gttttattgc cattccaaat ctgtcagcat ctgggtcatt
     4141 cataataata atatctgcat catgtttaat accatattca agcggtattt ttcatgcagg
     4201 atcaaattct ggatttggat ttacaacatt tttaaatgtt tcatcttcaa atgcatgctc
     4261 ttcaacctca ataacgttat atcctgattc acgtaatatt tttggggtaa atttagttcc
     4321 tgttccatta actgcgctaa aaataatttt taaatctttt ttagcttctt gctctttttt
     4381 gtacgtctct gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
     4441 ttgaaaaagt ggcaccgagt cggtgctttt ttgaattcga cattgattat tgactagtta
     4501 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     4561 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     4621 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     4681 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     4741 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     4801 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     4861 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     4921 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     4981 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     5041 ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt actggcttat
     5101 ctagagcgct gccaccatgg acaagaagta cagcatcggc ctggacatcg gcaccaactc
     5161 tgtgggctgg gccgtgatca ccgacgagta caaggtgccc agcaagaaat tcaaggtgct
     5221 gggcaacacc gaccggcaca gcatcaagaa gaacctgatc ggagccctgc tgttcgacag
     5281 cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc agaagaagat acaccagacg
     5341 gaagaaccgg atctgctatc tgcaagagat cttcagcaac gagatggcca aggtggacga
     5401 cagcttcttc cacagactgg aagagtcctt cctggtggaa gaggataaga agcacgagcg
     5461 gcaccccatc ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat
     5521 ctaccacctg agaaagaaac tggtggacag caccgacaag gccgacctgc ggctgatcta
     5581 tctggccctg gcccacatga tcaagttccg gggccacttc ctgatcgagg gcgacctgaa
     5641 ccccgacaac agcgacgtgg acaagctgtt catccagctg gtgcagacct acaaccagct
     5701 gttcgaggaa aaccccatca acgccagcgg cgtggacgcc aaggccatcc tgtctgccag
     5761 actgagcaag agcagacggc tggaaaatct gatcgcccag ctgcccggcg agaagaagaa
     5821 tggcctgttc ggaaacctga ttgccctgag cctgggcctg acccccaact tcaagagcaa
     5881 cttcgacctg gccgaggatg ccaaactgca gctgagcaag gacacctacg acgacgacct
     5941 ggacaacctg ctggcccaga tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa
     6001 cctgtccgac gccatcctgc tgagcgacat cctgagagtg aacaccgaga tcaccaaggc
     6061 ccccctgagc gcctctatga tcaagagata cgacgagcac caccaggacc tgaccctgct
     6121 gaaagctctc gtgcggcagc agctgcctga gaagtacaaa gagattttct tcgaccagag
     6181 caagaacggc tacgccggct acattgacgg cggagccagc caggaagagt tctacaagtt
     6241 catcaagccc atcctggaaa agatggacgg caccgaggaa ctgctcgtga agctgaacag
     6301 agaggacctg ctgcggaagc agcggacctt cgacaacggc agcatccccc accagatcca
     6361 cctgggagag ctgcacgcca ttctgcggcg gcaggaagat ttttacccat tcctgaagga
     6421 caaccgggaa aagatcgaga agatcctgac cttccgcatc ccctactacg tgggccctct
     6481 ggccagggga aacagcagat tcgcctggat gaccagaaag agcgaggaaa ccatcacccc
     6541 ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc cagagcttca tcgagcggat
     6601 gaccaacttc gataagaacc tgcccaacga gaaggtgctg cccaagcaca gcctgctgta
     6661 cgagtacttc accgtgtata acgagctgac caaagtgaaa tacgtgaccg agggaatgag
     6721 aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc tgttcaagac
     6781 caaccggaaa gtgaccgtga agcagctgaa agaggactac ttcaagaaaa tcgagtgctt
     6841 cgactccgtg gaaatctccg gcgtggaaga tcggttcaac gcctccctgg gcacatacca
     6901 cgatctgctg aaaattatca aggacaagga cttcctggac aatgaggaaa acgaggacat
     6961 tctggaagat atcgtgctga ccctgacact gtttgaggac agagagatga tcgaggaacg
     7021 gctgaaaacc tatgcccacc tgttcgacga caaagtgatg aagcagctga agcggcggag
     7081 atacaccggc tggggcaggc tgagccggaa gctgatcaac ggcatccggg acaagcagtc
     7141 cggcaagaca atcctggatt tcctgaagtc cgacggcttc gccaacagaa acttcatgca
     7201 gctgatccac gacgacagcc tgacctttaa agaggacatc cagaaagccc aggtgtccgg
     7261 ccagggcgat agcctgcacg agcacattgc caatctggcc ggcagccccg ccattaagaa
     7321 gggcatcctg cagacagtga aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa
     7381 gcccgagaac atcgtgatcg aaatggccag agagaaccag accacccaga agggacagaa
     7441 gaacagccgc gagagaatga agcggatcga agagggcatc aaagagctgg gcagccagat
     7501 cctgaaagaa caccccgtgg aaaacaccca gctgcagaac gagaagctgt acctgtacta
     7561 cctgcagaat gggcgggata tgtacgtgga ccaggaactg gacatcaacc ggctgtccga
     7621 ctacgatgtg gaccatatcg tgcctcagag ctttctgaag gacgactcca tcgacaacaa
     7681 ggtgctgacc agaagcgaca agaaccgggg caagagcgac aacgtgccct ccgaagaggt
     7741 cgtgaagaag atgaagaact actggcggca gctgctgaac gccaagctga ttacccagag
     7801 aaagttcgac aatctgacca aggccgagag aggcggcctg agcgaactgg ataaggccgg
     7861 cttcatcaag agacagctgg tggaaacccg gcagatcaca aagcacgtgg cacagatcct
     7921 ggactcccgg atgaacacta agtacgacga gaatgacaag ctgatccggg aagtgaaagt
     7981 gatcaccctg aagtccaagc tggtgtccga tttccggaag gatttccagt tttacaaagt
     8041 gcgcgagatc aacaactacc accacgccca cgacgcctac ctgaacgccg tcgtgggaac
     8101 cgccctgatc aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg actacaaggt
     8161 gtacgacgtg cggaagatga tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa
     8221 gtacttcttc tacagcaaca tcatgaactt tttcaagacc gagattaccc tggccaacgg
     8281 cgagatccgg aagcggcctc tgatcgagac aaacggcgaa accggggaga tcgtgtggga
     8341 taagggccgg gattttgcca ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt
     8401 gaaaaagacc gaggtgcaga caggcggctt cagcaaagag tctatcctgc ccaagaggaa
     8461 cagcgataag ctgatcgcca gaaagaagga ctgggaccct aagaagtacg gcggcttcga
     8521 cagccccacc gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa
     8581 gaaactgaag agtgtgaaag agctgctggg gatcaccatc atggaaagaa gcagcttcga
     8641 gaagaatccc atcgactttc tggaagccaa gggctacaaa gaagtgaaaa aggacctgat
     8701 catcaagctg cctaagtact ccctgttcga gctggaaaac ggccggaaga gaatgctggc
     8761 ctctgccggc gaactgcaga agggaaacga actggccctg ccctccaaat atgtgaactt
     8821 cctgtacctg gccagccact atgagaagct gaagggctcc cccgaggata atgagcagaa
     8881 acagctgttt gtggaacagc acaagcacta cctggacgag atcatcgagc agatcagcga
     8941 gttctccaag agagtgatcc tggccgacgc taatctggac aaagtgctgt ccgcctacaa
     9001 caagcaccgg gataagccca tcagagagca ggccgagaat atcatccacc tgtttaccct
     9061 gaccaatctg ggagcccctg ccgccttcaa gtactttgac accaccatcg accggaagag
     9121 gtacaccagc accaaagagg tgctggacgc caccctgatc caccagagca tcaccggcct
     9181 gtacgagaca cggatcgacc tgtctcagct gggaggcgac aagcgacctg ccgccacaaa
     9241 gaaggctgga caggctaaga agaagaaaga ttacaaagac gatgacgata agggatccgg
     9301 cgcaacaaac ttctctctgc tgaaacaagc cggagatgtc gaagagaatc ctggaccgat
     9361 ggcccagtcc aagcacggcc tgaccaagga gatgaccatg aagtaccgca tggagggctg
     9421 cgtggacggc cacaagttcg tgatcaccgg cgagggcatc ggctacccct tcaagggcaa
     9481 gcaggccatc aacctgtgcg tggtggaggg cggccccttg cccttcgccg aggacatctt
     9541 gtccgccgcc ttcatgtacg gcaaccgcgt gttcaccgag tacccccagg acatcgtcga
     9601 ctacttcaag aactcctgcc ccgccggcta cacctgggac cgctccttcc tgttcgagga
     9661 cggcgccgtg tgcatctgca acgccgacat caccgtgagc gtggaggaga actgcatgta
     9721 ccacgagtcc aagttctacg gcgtgaactt ccccgccgac ggccccgtga tgaagaagat
     9781 gaccgacaac tgggagccct cctgcgagaa gatcatcccc gtgcccaagc agggcatctt
     9841 gaagggcgac gtgagcatgt acctgctgct gaaggacggt ggccgcttgc gctgccagtt
     9901 cgacaccgtg tacaaggcca agtccgtgcc ccgcaagatg cccgactggc acttcatcca
     9961 gcacaagctg acccgcgagg accgcagcga cgccaagaac cagaagtggc acctgaccga
    10021 gcacgccatc gcctccggct ccgccttgcc ctgaacgcgt ctggaacaat caacctctgg
    10081 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
    10141 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
    10201 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
    10261 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
    10321 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
    10381 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
    10441 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
    10501 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
    10561 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
    10621 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct gcagtcgaga
    10681 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
    10741 ctggctagaa gcacaagagg aggaggaggt gggtttttcc agtcacacct caggtacctt
    10801 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaagaggg
    10861 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
    10921 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
    10981 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
    11041 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
    11101 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
    11161 gtgagaggcc ttgacattgt ttaaactgga atgtgtgtca gttagggtgt ggaaagtccc
    11221 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
    11281 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
    11341 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
    11401 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
    11461 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
    11521 aaaagctccc gggagcttgt atatccatta catgtgagca aaaggccagc aaaaggccag
    11581 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    11641 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    11701 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    11761 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    11821 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    11881 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    11941 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    12001 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    12061 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    12121 cggcaaacaa accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag
    12181 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    12241 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    12301 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    12361 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    12421 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    12481 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    12541 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    12601 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    12661 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    12721 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    12781 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    12841 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    12901 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    12961 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    13021 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    13081 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    13141 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    13201 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    13261 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    13321 aggggttccg cgcacatttc cccgaaaagt gccacctgac gt
//
