LOCUS       V012252                13130 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V012252
VERSION     V012252
KEYWORDS    pCLIP-All-TRE3G-tRFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13130)
  AUTHORS   transOMIC
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 13130)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..404
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             411..668
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    715..840
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1333..1566
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1751..1795
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1944..1985
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2093..2210
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2261..2501
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    2506..4390
                     /label="Stuffer"
                     /note="Stuffer"
     misc_RNA        4391..4466
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     terminator      4467..4472
                     /note="polIII terminator"
                     /note="RNA polymerase III transcription terminator"
     promoter        4501..4868
                     /label="TRE3GS promoter"
                     /note="3rd-generation Tet-responsive promoter that can be
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription
                     factors"
     CDS             4886..8989
                     /label="Cas9"
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
     CDS             8990..9037
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label="bipartite nuclear localization signal from nucl"
                     /note="nucleoplasmin NLS"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             9038..9061
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             9071..9127
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label="2A peptide from porcine teschovirus-1 polyprotein"
                     /note="P2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9128..9820
                     /label="TurboRFP"
                     /note="red fluorescent protein from Entacmaea quadricolor"
     CDS             complement(10307..10318)
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     LTR             10632..10865
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        10961..11290
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(11379..11964)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(12138..12995)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(12996..13100)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatcctcg cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accagctagc gaggcgtggc
      421 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
      481 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
      541 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
      601 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
      661 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
      721 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
      781 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
      841 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
      901 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
      961 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     1021 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     1081 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     1141 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     1201 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1261 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1321 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1381 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     1441 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     1501 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     1561 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     1621 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     1681 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     1741 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     1801 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     1861 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     1921 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     1981 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     2041 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     2101 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2161 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     2221 acagggacag cagagatcca gtttggttaa ttaaggtacc gagggcctat ttcccatgat
     2281 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac
     2341 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
     2401 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa
     2461 gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg gagacggttg
     2521 taaatgagca cacaaaatac acatgctaaa atattatatt ctatgacctt tataaaatca
     2581 accaaaatct tctttttaat aactttagta tcaataatta gaatttttat gttccttttt
     2641 gcaaactttt aataaaaatg agcaaaataa aaaaacgcta gttttagtaa ctcgcgttgt
     2701 tttcttcacc tttaataata gctactccac cacttgttcc taagcggtca gctcctgctt
     2761 caatcatttt ttgagcatct tcaaatgttc taactccacc agctgcttta actaaagcat
     2821 tgtctttaac aactgacttc attagtttaa catcttcaaa tgttgcacct gattttgaaa
     2881 atcctgttga tgttttaaca aattctaatc cagcttcaac agctatttca caagctttca
     2941 tgatttcttc ttttgttaat aaacaatttt ccataataca tttaacaaca tgtgatccag
     3001 ctgctttttt tacagctttc atgtcttcta aaactaattc ataatttttg tcttttaatg
     3061 caccaatatt taataccata tcaatttctg ttgcaccatc tttaattgct tcagaaactt
     3121 cgaatgcttt tgtagctgtt gtgcatgcac ctagaggaaa acctacaaca tttgttattc
     3181 ctacatttgt gccttttaat aattctttac aatagcttgt tcaatatgaa ttaacacaaa
     3241 ctgttgcaaa atcaaattca attgcttcat cacataattg tttaatttca gctttcgtag
     3301 catcttgttt taataatgtg tgatctatat atttgtttag tttcattttt tctcctatat
     3361 attcattttt aattttaatt ctttaataat ttcgtctact ttaactttag cgttttgaac
     3421 agattcacca acacctataa aataaatttt tagtttaggt tcagttccac ttgggcgaac
     3481 agcaaatcat gacttatctt ctaaataaaa ttttagtaag tcttgtcctg gcatattata
     3541 cattccatcg atgtagtctt caacattaac aactttaagt ccagcaattt gagttaaggg
     3601 tgttgctctc aatgatttca ttaatggttc aatttttaat ttcttttctt ctggtttaaa
     3661 attcaagttt aaagtgaaag tgtaatatgc acccatttct ttaaataaat cttctaaata
     3721 gtctactaat gttttatttt gttttttata aaatcaagca gcctctgcta ttaatataga
     3781 agcttgtatt ccatctttat ctctagctga gtcatcaatt acatatccat aactttcttc
     3841 ataagcaaaa acaaaattta atccgttatc ttcttcttta gcaatttctc tacccattca
     3901 tttaaatcca gttaaagttt ttacaatatt aactccatat ttttcatgag cgattctatc
     3961 acccaaatca cttgttacaa aacttgaata tagagccgga ttttttggaa tgctatttaa
     4021 gcgttttaga tttgataatt ttcaatcaat taaaattggt cctgtttgat ttccatctaa
     4081 tcttacaaaa tgaccatcat gttttattgc cattccaaat ctgtcagcat ctgggtcatt
     4141 cataataata atatctgcat catgtttaat accatattca agcggtattt ttcatgcagg
     4201 atcaaattct ggatttggat ttacaacatt tttaaatgtt tcatcttcaa atgcatgctc
     4261 ttcaacctca ataacgttat atcctgattc acgtaatatt tttggggtaa atttagttcc
     4321 tgttccatta actgcgctaa aaataatttt taaatctttt ttagcttctt gctctttttt
     4381 gtacgtctct gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
     4441 ttgaaaaagt ggcaccgagt cggtgctttt ttgaattcaa ccccgtggaa ttatcacctc
     4501 gagtttactc cctatcagtg atagagaacg tatgaagagt ttactcccta tcagtgatag
     4561 agaacgtatg cagactttac tccctatcag tgatagagaa cgtataagga gtttactccc
     4621 tatcagtgat agagaacgta tgaccagttt actccctatc agtgatagag aacgtatcta
     4681 cagtttactc cctatcagtg atagagaacg tatatccagt ttactcccta tcagtgatag
     4741 agaacgtata agctttgctt atgtaaacca gggcgcctat aaaagagtgc tgattttttg
     4801 agtaaacttc aattccacaa cacttttgtc ttataccaac tttccgtacc acttcctacc
     4861 ctcgtaaatc tagagcgctg ccaccatgga caagaagtac agcatcggcc tggacatcgg
     4921 caccaactct gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt
     4981 caaggtgctg ggcaacaccg accggcacag catcaagaag aacctgatcg gagccctgct
     5041 gttcgacagc ggcgaaacag ccgaggccac ccggctgaag agaaccgcca gaagaagata
     5101 caccagacgg aagaaccgga tctgctatct gcaagagatc ttcagcaacg agatggccaa
     5161 ggtggacgac agcttcttcc acagactgga agagtccttc ctggtggaag aggataagaa
     5221 gcacgagcgg caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta
     5281 ccccaccatc taccacctga gaaagaaact ggtggacagc accgacaagg ccgacctgcg
     5341 gctgatctat ctggccctgg cccacatgat caagttccgg ggccacttcc tgatcgaggg
     5401 cgacctgaac cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta
     5461 caaccagctg ttcgaggaaa accccatcaa cgccagcggc gtggacgcca aggccatcct
     5521 gtctgccaga ctgagcaaga gcagacggct ggaaaatctg atcgcccagc tgcccggcga
     5581 gaagaagaat ggcctgttcg gaaacctgat tgccctgagc ctgggcctga cccccaactt
     5641 caagagcaac ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg acacctacga
     5701 cgacgacctg gacaacctgc tggcccagat cggcgaccag tacgccgacc tgtttctggc
     5761 cgccaagaac ctgtccgacg ccatcctgct gagcgacatc ctgagagtga acaccgagat
     5821 caccaaggcc cccctgagcg cctctatgat caagagatac gacgagcacc accaggacct
     5881 gaccctgctg aaagctctcg tgcggcagca gctgcctgag aagtacaaag agattttctt
     5941 cgaccagagc aagaacggct acgccggcta cattgacggc ggagccagcc aggaagagtt
     6001 ctacaagttc atcaagccca tcctggaaaa gatggacggc accgaggaac tgctcgtgaa
     6061 gctgaacaga gaggacctgc tgcggaagca gcggaccttc gacaacggca gcatccccca
     6121 ccagatccac ctgggagagc tgcacgccat tctgcggcgg caggaagatt tttacccatt
     6181 cctgaaggac aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc cctactacgt
     6241 gggccctctg gccaggggaa acagcagatt cgcctggatg accagaaaga gcgaggaaac
     6301 catcaccccc tggaacttcg aggaagtggt ggacaagggc gcttccgccc agagcttcat
     6361 cgagcggatg accaacttcg ataagaacct gcccaacgag aaggtgctgc ccaagcacag
     6421 cctgctgtac gagtacttca ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga
     6481 gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg tggacctgct
     6541 gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat
     6601 cgagtgcttc gactccgtgg aaatctccgg cgtggaagat cggttcaacg cctccctggg
     6661 cacataccac gatctgctga aaattatcaa ggacaaggac ttcctggaca atgaggaaaa
     6721 cgaggacatt ctggaagata tcgtgctgac cctgacactg tttgaggaca gagagatgat
     6781 cgaggaacgg ctgaaaacct atgcccacct gttcgacgac aaagtgatga agcagctgaa
     6841 gcggcggaga tacaccggct ggggcaggct gagccggaag ctgatcaacg gcatccggga
     6901 caagcagtcc ggcaagacaa tcctggattt cctgaagtcc gacggcttcg ccaacagaaa
     6961 cttcatgcag ctgatccacg acgacagcct gacctttaaa gaggacatcc agaaagccca
     7021 ggtgtccggc cagggcgata gcctgcacga gcacattgcc aatctggccg gcagccccgc
     7081 cattaagaag ggcatcctgc agacagtgaa ggtggtggac gagctcgtga aagtgatggg
     7141 ccggcacaag cccgagaaca tcgtgatcga aatggccaga gagaaccaga ccacccagaa
     7201 gggacagaag aacagccgcg agagaatgaa gcggatcgaa gagggcatca aagagctggg
     7261 cagccagatc ctgaaagaac accccgtgga aaacacccag ctgcagaacg agaagctgta
     7321 cctgtactac ctgcagaatg ggcgggatat gtacgtggac caggaactgg acatcaaccg
     7381 gctgtccgac tacgatgtgg accatatcgt gcctcagagc tttctgaagg acgactccat
     7441 cgacaacaag gtgctgacca gaagcgacaa gaaccggggc aagagcgaca acgtgccctc
     7501 cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg ccaagctgat
     7561 tacccagaga aagttcgaca atctgaccaa ggccgagaga ggcggcctga gcgaactgga
     7621 taaggccggc ttcatcaaga gacagctggt ggaaacccgg cagatcacaa agcacgtggc
     7681 acagatcctg gactcccgga tgaacactaa gtacgacgag aatgacaagc tgatccggga
     7741 agtgaaagtg atcaccctga agtccaagct ggtgtccgat ttccggaagg atttccagtt
     7801 ttacaaagtg cgcgagatca acaactacca ccacgcccac gacgcctacc tgaacgccgt
     7861 cgtgggaacc gccctgatca aaaagtaccc taagctggaa agcgagttcg tgtacggcga
     7921 ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc
     7981 taccgccaag tacttcttct acagcaacat catgaacttt ttcaagaccg agattaccct
     8041 ggccaacggc gagatccgga agcggcctct gatcgagaca aacggcgaaa ccggggagat
     8101 cgtgtgggat aagggccggg attttgccac cgtgcggaaa gtgctgagca tgccccaagt
     8161 gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc agcaaagagt ctatcctgcc
     8221 caagaggaac agcgataagc tgatcgccag aaagaaggac tgggacccta agaagtacgg
     8281 cggcttcgac agccccaccg tggcctattc tgtgctggtg gtggccaaag tggaaaaggg
     8341 caagtccaag aaactgaaga gtgtgaaaga gctgctgggg atcaccatca tggaaagaag
     8401 cagcttcgag aagaatccca tcgactttct ggaagccaag ggctacaaag aagtgaaaaa
     8461 ggacctgatc atcaagctgc ctaagtactc cctgttcgag ctggaaaacg gccggaagag
     8521 aatgctggcc tctgccggcg aactgcagaa gggaaacgaa ctggccctgc cctccaaata
     8581 tgtgaacttc ctgtacctgg ccagccacta tgagaagctg aagggctccc ccgaggataa
     8641 tgagcagaaa cagctgtttg tggaacagca caagcactac ctggacgaga tcatcgagca
     8701 gatcagcgag ttctccaaga gagtgatcct ggccgacgct aatctggaca aagtgctgtc
     8761 cgcctacaac aagcaccggg ataagcccat cagagagcag gccgagaata tcatccacct
     8821 gtttaccctg accaatctgg gagcccctgc cgccttcaag tactttgaca ccaccatcga
     8881 ccggaagagg tacaccagca ccaaagaggt gctggacgcc accctgatcc accagagcat
     8941 caccggcctg tacgagacac ggatcgacct gtctcagctg ggaggcgaca agcgacctgc
     9001 cgccacaaag aaggctggac aggctaagaa gaagaaagat tacaaagacg atgacgataa
     9061 gggatccggc gcaacaaact tctctctgct gaaacaagcc ggagatgtcg aagagaatcc
     9121 tggaccgatg agcgagctga tcaaggagaa catgcacatg aagctgtaca tggagggcac
     9181 cgtgaacaac caccacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac
     9241 ccagaccatg aagatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct
     9301 ggctaccagc ttcatgtacg gcagcaaagc cttcatcaac cacacccagg gcatccccga
     9361 cttctttaag cagtccttcc ctgagggctt cacatgggag agaatcacca catacgaaga
     9421 cgggggcgtg ctgaccgcta cccaggacac cagcttccag aacggctgca tcatctacaa
     9481 cgtcaagatc aacggggtga acttcccatc caacggccct gtgatgcaga agaaaacacg
     9541 cggctgggag gccaacaccg agatgctgta ccccgctgac ggcggcctga gaggccacag
     9601 ccagatggcc ctgaagctcg tgggcggggg ctacctgcac tgctccttca agaccacata
     9661 cagatccaag aaacccgcta agaacctcaa gatgcccggc ttccacttcg tggaccacag
     9721 actggaaaga atcaaggagg ccgacaaaga gacctacgtc gagcagcacg agatggctgt
     9781 ggccaagtac tgcgacctcc ctagcaaact ggggcacaga taacgcgtct ggaacaatca
     9841 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     9901 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     9961 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
    10021 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
    10081 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
    10141 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
    10201 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
    10261 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
    10321 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
    10381 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc
    10441 agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
    10501 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gtttttccag tcacacctca
    10561 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
    10621 aaagagggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
    10681 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
    10741 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
    10801 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
    10861 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
    10921 atcagagagt gagaggcctt gacattgttt aaactggaat gtgtgtcagt tagggtgtgg
    10981 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
    11041 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
    11101 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
    11161 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
    11221 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
    11281 cttttgcaaa aagctcccgg gagcttgtat atccattaca tgtgagcaaa aggccagcaa
    11341 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
    11401 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
    11461 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
    11521 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
    11581 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
    11641 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    11701 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    11761 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
    11821 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    11881 tcttgatccg gcaaacaaac caccgctggt agcggttttt ttgtttgcaa gcagcagatt
    11941 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    12001 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    12061 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    12121 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    12181 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    12241 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    12301 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    12361 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    12421 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    12481 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    12541 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    12601 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    12661 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    12721 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    12781 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    12841 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    12901 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    12961 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    13021 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    13081 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
//
