LOCUS       V012253                13032 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V012253
VERSION     V012253
KEYWORDS    pCLIP-All-TRE3G-Puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13032)
  AUTHORS   transOMIC
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 13032)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13032
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..404
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             411..668
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    715..840
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1333..1566
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1751..1795
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1944..1985
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2093..2210
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2261..2501
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    2506..4390
                     /label="Stuffer"
                     /note="Stuffer"
     misc_RNA        4391..4466
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     terminator      4467..4472
                     /note="polIII terminator"
                     /note="RNA polymerase III transcription terminator"
     promoter        4501..4868
                     /label="TRE3GS promoter"
                     /note="3rd-generation Tet-responsive promoter that can be
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription
                     factors"
     CDS             4886..8989
                     /label="Cas9"
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
     CDS             8990..9037
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label="bipartite nuclear localization signal from nucl"
                     /note="nucleoplasmin NLS"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             9038..9061
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             9071..9127
                     /label="P2A"
                     /note="2A peptide from porcine teschovirus-1 polyprotein"
     CDS             9128..9721
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     CDS             complement(10209..10220)
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     LTR             10534..10767
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        10863..11192
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(11281..11866)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(12040..12897)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(12898..13002)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatcctcg cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accagctagc gaggcgtggc
      421 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
      481 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
      541 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
      601 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
      661 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
      721 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
      781 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
      841 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
      901 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
      961 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     1021 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     1081 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     1141 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     1201 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1261 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1321 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1381 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     1441 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     1501 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     1561 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     1621 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     1681 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     1741 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     1801 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     1861 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     1921 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     1981 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     2041 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     2101 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2161 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     2221 acagggacag cagagatcca gtttggttaa ttaaggtacc gagggcctat ttcccatgat
     2281 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac
     2341 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
     2401 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa
     2461 gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg gagacggttg
     2521 taaatgagca cacaaaatac acatgctaaa atattatatt ctatgacctt tataaaatca
     2581 accaaaatct tctttttaat aactttagta tcaataatta gaatttttat gttccttttt
     2641 gcaaactttt aataaaaatg agcaaaataa aaaaacgcta gttttagtaa ctcgcgttgt
     2701 tttcttcacc tttaataata gctactccac cacttgttcc taagcggtca gctcctgctt
     2761 caatcatttt ttgagcatct tcaaatgttc taactccacc agctgcttta actaaagcat
     2821 tgtctttaac aactgacttc attagtttaa catcttcaaa tgttgcacct gattttgaaa
     2881 atcctgttga tgttttaaca aattctaatc cagcttcaac agctatttca caagctttca
     2941 tgatttcttc ttttgttaat aaacaatttt ccataataca tttaacaaca tgtgatccag
     3001 ctgctttttt tacagctttc atgtcttcta aaactaattc ataatttttg tcttttaatg
     3061 caccaatatt taataccata tcaatttctg ttgcaccatc tttaattgct tcagaaactt
     3121 cgaatgcttt tgtagctgtt gtgcatgcac ctagaggaaa acctacaaca tttgttattc
     3181 ctacatttgt gccttttaat aattctttac aatagcttgt tcaatatgaa ttaacacaaa
     3241 ctgttgcaaa atcaaattca attgcttcat cacataattg tttaatttca gctttcgtag
     3301 catcttgttt taataatgtg tgatctatat atttgtttag tttcattttt tctcctatat
     3361 attcattttt aattttaatt ctttaataat ttcgtctact ttaactttag cgttttgaac
     3421 agattcacca acacctataa aataaatttt tagtttaggt tcagttccac ttgggcgaac
     3481 agcaaatcat gacttatctt ctaaataaaa ttttagtaag tcttgtcctg gcatattata
     3541 cattccatcg atgtagtctt caacattaac aactttaagt ccagcaattt gagttaaggg
     3601 tgttgctctc aatgatttca ttaatggttc aatttttaat ttcttttctt ctggtttaaa
     3661 attcaagttt aaagtgaaag tgtaatatgc acccatttct ttaaataaat cttctaaata
     3721 gtctactaat gttttatttt gttttttata aaatcaagca gcctctgcta ttaatataga
     3781 agcttgtatt ccatctttat ctctagctga gtcatcaatt acatatccat aactttcttc
     3841 ataagcaaaa acaaaattta atccgttatc ttcttcttta gcaatttctc tacccattca
     3901 tttaaatcca gttaaagttt ttacaatatt aactccatat ttttcatgag cgattctatc
     3961 acccaaatca cttgttacaa aacttgaata tagagccgga ttttttggaa tgctatttaa
     4021 gcgttttaga tttgataatt ttcaatcaat taaaattggt cctgtttgat ttccatctaa
     4081 tcttacaaaa tgaccatcat gttttattgc cattccaaat ctgtcagcat ctgggtcatt
     4141 cataataata atatctgcat catgtttaat accatattca agcggtattt ttcatgcagg
     4201 atcaaattct ggatttggat ttacaacatt tttaaatgtt tcatcttcaa atgcatgctc
     4261 ttcaacctca ataacgttat atcctgattc acgtaatatt tttggggtaa atttagttcc
     4321 tgttccatta actgcgctaa aaataatttt taaatctttt ttagcttctt gctctttttt
     4381 gtacgtctct gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
     4441 ttgaaaaagt ggcaccgagt cggtgctttt ttgaattcaa ccccgtggaa ttatcacctc
     4501 gagtttactc cctatcagtg atagagaacg tatgaagagt ttactcccta tcagtgatag
     4561 agaacgtatg cagactttac tccctatcag tgatagagaa cgtataagga gtttactccc
     4621 tatcagtgat agagaacgta tgaccagttt actccctatc agtgatagag aacgtatcta
     4681 cagtttactc cctatcagtg atagagaacg tatatccagt ttactcccta tcagtgatag
     4741 agaacgtata agctttgctt atgtaaacca gggcgcctat aaaagagtgc tgattttttg
     4801 agtaaacttc aattccacaa cacttttgtc ttataccaac tttccgtacc acttcctacc
     4861 ctcgtaaatc tagagcgctg ccaccatgga caagaagtac agcatcggcc tggacatcgg
     4921 caccaactct gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt
     4981 caaggtgctg ggcaacaccg accggcacag catcaagaag aacctgatcg gagccctgct
     5041 gttcgacagc ggcgaaacag ccgaggccac ccggctgaag agaaccgcca gaagaagata
     5101 caccagacgg aagaaccgga tctgctatct gcaagagatc ttcagcaacg agatggccaa
     5161 ggtggacgac agcttcttcc acagactgga agagtccttc ctggtggaag aggataagaa
     5221 gcacgagcgg caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta
     5281 ccccaccatc taccacctga gaaagaaact ggtggacagc accgacaagg ccgacctgcg
     5341 gctgatctat ctggccctgg cccacatgat caagttccgg ggccacttcc tgatcgaggg
     5401 cgacctgaac cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta
     5461 caaccagctg ttcgaggaaa accccatcaa cgccagcggc gtggacgcca aggccatcct
     5521 gtctgccaga ctgagcaaga gcagacggct ggaaaatctg atcgcccagc tgcccggcga
     5581 gaagaagaat ggcctgttcg gaaacctgat tgccctgagc ctgggcctga cccccaactt
     5641 caagagcaac ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg acacctacga
     5701 cgacgacctg gacaacctgc tggcccagat cggcgaccag tacgccgacc tgtttctggc
     5761 cgccaagaac ctgtccgacg ccatcctgct gagcgacatc ctgagagtga acaccgagat
     5821 caccaaggcc cccctgagcg cctctatgat caagagatac gacgagcacc accaggacct
     5881 gaccctgctg aaagctctcg tgcggcagca gctgcctgag aagtacaaag agattttctt
     5941 cgaccagagc aagaacggct acgccggcta cattgacggc ggagccagcc aggaagagtt
     6001 ctacaagttc atcaagccca tcctggaaaa gatggacggc accgaggaac tgctcgtgaa
     6061 gctgaacaga gaggacctgc tgcggaagca gcggaccttc gacaacggca gcatccccca
     6121 ccagatccac ctgggagagc tgcacgccat tctgcggcgg caggaagatt tttacccatt
     6181 cctgaaggac aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc cctactacgt
     6241 gggccctctg gccaggggaa acagcagatt cgcctggatg accagaaaga gcgaggaaac
     6301 catcaccccc tggaacttcg aggaagtggt ggacaagggc gcttccgccc agagcttcat
     6361 cgagcggatg accaacttcg ataagaacct gcccaacgag aaggtgctgc ccaagcacag
     6421 cctgctgtac gagtacttca ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga
     6481 gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg tggacctgct
     6541 gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat
     6601 cgagtgcttc gactccgtgg aaatctccgg cgtggaagat cggttcaacg cctccctggg
     6661 cacataccac gatctgctga aaattatcaa ggacaaggac ttcctggaca atgaggaaaa
     6721 cgaggacatt ctggaagata tcgtgctgac cctgacactg tttgaggaca gagagatgat
     6781 cgaggaacgg ctgaaaacct atgcccacct gttcgacgac aaagtgatga agcagctgaa
     6841 gcggcggaga tacaccggct ggggcaggct gagccggaag ctgatcaacg gcatccggga
     6901 caagcagtcc ggcaagacaa tcctggattt cctgaagtcc gacggcttcg ccaacagaaa
     6961 cttcatgcag ctgatccacg acgacagcct gacctttaaa gaggacatcc agaaagccca
     7021 ggtgtccggc cagggcgata gcctgcacga gcacattgcc aatctggccg gcagccccgc
     7081 cattaagaag ggcatcctgc agacagtgaa ggtggtggac gagctcgtga aagtgatggg
     7141 ccggcacaag cccgagaaca tcgtgatcga aatggccaga gagaaccaga ccacccagaa
     7201 gggacagaag aacagccgcg agagaatgaa gcggatcgaa gagggcatca aagagctggg
     7261 cagccagatc ctgaaagaac accccgtgga aaacacccag ctgcagaacg agaagctgta
     7321 cctgtactac ctgcagaatg ggcgggatat gtacgtggac caggaactgg acatcaaccg
     7381 gctgtccgac tacgatgtgg accatatcgt gcctcagagc tttctgaagg acgactccat
     7441 cgacaacaag gtgctgacca gaagcgacaa gaaccggggc aagagcgaca acgtgccctc
     7501 cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg ccaagctgat
     7561 tacccagaga aagttcgaca atctgaccaa ggccgagaga ggcggcctga gcgaactgga
     7621 taaggccggc ttcatcaaga gacagctggt ggaaacccgg cagatcacaa agcacgtggc
     7681 acagatcctg gactcccgga tgaacactaa gtacgacgag aatgacaagc tgatccggga
     7741 agtgaaagtg atcaccctga agtccaagct ggtgtccgat ttccggaagg atttccagtt
     7801 ttacaaagtg cgcgagatca acaactacca ccacgcccac gacgcctacc tgaacgccgt
     7861 cgtgggaacc gccctgatca aaaagtaccc taagctggaa agcgagttcg tgtacggcga
     7921 ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc
     7981 taccgccaag tacttcttct acagcaacat catgaacttt ttcaagaccg agattaccct
     8041 ggccaacggc gagatccgga agcggcctct gatcgagaca aacggcgaaa ccggggagat
     8101 cgtgtgggat aagggccggg attttgccac cgtgcggaaa gtgctgagca tgccccaagt
     8161 gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc agcaaagagt ctatcctgcc
     8221 caagaggaac agcgataagc tgatcgccag aaagaaggac tgggacccta agaagtacgg
     8281 cggcttcgac agccccaccg tggcctattc tgtgctggtg gtggccaaag tggaaaaggg
     8341 caagtccaag aaactgaaga gtgtgaaaga gctgctgggg atcaccatca tggaaagaag
     8401 cagcttcgag aagaatccca tcgactttct ggaagccaag ggctacaaag aagtgaaaaa
     8461 ggacctgatc atcaagctgc ctaagtactc cctgttcgag ctggaaaacg gccggaagag
     8521 aatgctggcc tctgccggcg aactgcagaa gggaaacgaa ctggccctgc cctccaaata
     8581 tgtgaacttc ctgtacctgg ccagccacta tgagaagctg aagggctccc ccgaggataa
     8641 tgagcagaaa cagctgtttg tggaacagca caagcactac ctggacgaga tcatcgagca
     8701 gatcagcgag ttctccaaga gagtgatcct ggccgacgct aatctggaca aagtgctgtc
     8761 cgcctacaac aagcaccggg ataagcccat cagagagcag gccgagaata tcatccacct
     8821 gtttaccctg accaatctgg gagcccctgc cgccttcaag tactttgaca ccaccatcga
     8881 ccggaagagg tacaccagca ccaaagaggt gctggacgcc accctgatcc accagagcat
     8941 caccggcctg tacgagacac ggatcgacct gtctcagctg ggaggcgaca agcgacctgc
     9001 cgccacaaag aaggctggac aggctaagaa gaagaaagat tacaaagacg atgacgataa
     9061 gggatccggc gcaacaaact tctctctgct gaaacaagcc ggagatgtcg aagagaatcc
     9121 tggaccgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     9181 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     9241 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     9301 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     9361 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     9421 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     9481 ggagcccgcg tggttcctgg ccaccgtcgg agtctcgccc gaccaccagg gcaagggtct
     9541 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     9601 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     9661 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     9721 ctgaacgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     9781 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     9841 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     9901 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     9961 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
    10021 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
    10081 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
    10141 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
    10201 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
    10261 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
    10321 ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa tcacaagtag
    10381 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
    10441 gggtttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
    10501 atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac tcccaacgaa
    10561 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
    10621 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
    10681 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
    10741 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
    10801 tataacttgc aaagaaatga atatcagaga gtgagaggcc ttgacattgt ttaaactgga
    10861 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
    10921 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
    10981 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
    11041 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
    11101 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
    11161 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccatta
    11221 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    11281 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    11341 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    11401 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    11461 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    11521 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    11581 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    11641 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    11701 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
    11761 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggttt
    11821 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    11881 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    11941 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    12001 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    12061 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    12121 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    12181 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    12241 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    12301 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    12361 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    12421 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    12481 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    12541 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    12601 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    12661 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    12721 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    12781 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    12841 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    12901 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    12961 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    13021 gccacctgac gt
//
