LOCUS       V012259                13368 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V012259
VERSION     V012259
KEYWORDS    pCLIP-All-Nickase(D10A)-hCMV-ZsGreen
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13368)
  AUTHORS   transOMIC
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 13368)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..404
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             411..668
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    715..840
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1333..1566
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1751..1795
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1944..1985
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2093..2210
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2261..2501
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    2506..4390
                     /label="Stuffer"
                     /note="Stuffer"
     misc_RNA        4391..4466
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     terminator      4467..4472
                     /note="polIII terminator"
                     /note="RNA polymerase III transcription terminator"
     enhancer        4479..4858
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4859..5062
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             5123..9226
                     /label="Cas9(D10A)"
                     /note="nickase mutant of the Cas9 endonuclease from the
                     Streptococcus pyogenes Type II CRISPR/Cas system"
     CDS             9227..9274
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label="bipartite nuclear localization signal from nucl"
                     /note="nucleoplasmin NLS"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             9275..9298
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             9308..9364
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label="2A peptide from porcine teschovirus-1 polyprotein"
                     /note="P2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9365..10057
                     /label="ZsGreen1"
                     /note="Zoanthus green fluorescent protein"
     misc_feature    10074..10662
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             10870..11103
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        11199..11528
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(11617..12202)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(12376..13233)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(13234..13338)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatcctcg cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accagctagc gaggcgtggc
      421 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
      481 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
      541 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
      601 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
      661 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
      721 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
      781 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
      841 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
      901 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
      961 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     1021 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     1081 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     1141 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     1201 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1261 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1321 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1381 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     1441 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     1501 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     1561 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     1621 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     1681 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     1741 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     1801 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     1861 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     1921 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     1981 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     2041 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     2101 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2161 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     2221 acagggacag cagagatcca gtttggttaa ttaaggtacc gagggcctat ttcccatgat
     2281 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac
     2341 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
     2401 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa
     2461 gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg gagacggttg
     2521 taaatgagca cacaaaatac acatgctaaa atattatatt ctatgacctt tataaaatca
     2581 accaaaatct tctttttaat aactttagta tcaataatta gaatttttat gttccttttt
     2641 gcaaactttt aataaaaatg agcaaaataa aaaaacgcta gttttagtaa ctcgcgttgt
     2701 tttcttcacc tttaataata gctactccac cacttgttcc taagcggtca gctcctgctt
     2761 caatcatttt ttgagcatct tcaaatgttc taactccacc agctgcttta actaaagcat
     2821 tgtctttaac aactgacttc attagtttaa catcttcaaa tgttgcacct gattttgaaa
     2881 atcctgttga tgttttaaca aattctaatc cagcttcaac agctatttca caagctttca
     2941 tgatttcttc ttttgttaat aaacaatttt ccataataca tttaacaaca tgtgatccag
     3001 ctgctttttt tacagctttc atgtcttcta aaactaattc ataatttttg tcttttaatg
     3061 caccaatatt taataccata tcaatttctg ttgcaccatc tttaattgct tcagaaactt
     3121 cgaatgcttt tgtagctgtt gtgcatgcac ctagaggaaa acctacaaca tttgttattc
     3181 ctacatttgt gccttttaat aattctttac aatagcttgt tcaatatgaa ttaacacaaa
     3241 ctgttgcaaa atcaaattca attgcttcat cacataattg tttaatttca gctttcgtag
     3301 catcttgttt taataatgtg tgatctatat atttgtttag tttcattttt tctcctatat
     3361 attcattttt aattttaatt ctttaataat ttcgtctact ttaactttag cgttttgaac
     3421 agattcacca acacctataa aataaatttt tagtttaggt tcagttccac ttgggcgaac
     3481 agcaaatcat gacttatctt ctaaataaaa ttttagtaag tcttgtcctg gcatattata
     3541 cattccatcg atgtagtctt caacattaac aactttaagt ccagcaattt gagttaaggg
     3601 tgttgctctc aatgatttca ttaatggttc aatttttaat ttcttttctt ctggtttaaa
     3661 attcaagttt aaagtgaaag tgtaatatgc acccatttct ttaaataaat cttctaaata
     3721 gtctactaat gttttatttt gttttttata aaatcaagca gcctctgcta ttaatataga
     3781 agcttgtatt ccatctttat ctctagctga gtcatcaatt acatatccat aactttcttc
     3841 ataagcaaaa acaaaattta atccgttatc ttcttcttta gcaatttctc tacccattca
     3901 tttaaatcca gttaaagttt ttacaatatt aactccatat ttttcatgag cgattctatc
     3961 acccaaatca cttgttacaa aacttgaata tagagccgga ttttttggaa tgctatttaa
     4021 gcgttttaga tttgataatt ttcaatcaat taaaattggt cctgtttgat ttccatctaa
     4081 tcttacaaaa tgaccatcat gttttattgc cattccaaat ctgtcagcat ctgggtcatt
     4141 cataataata atatctgcat catgtttaat accatattca agcggtattt ttcatgcagg
     4201 atcaaattct ggatttggat ttacaacatt tttaaatgtt tcatcttcaa atgcatgctc
     4261 ttcaacctca ataacgttat atcctgattc acgtaatatt tttggggtaa atttagttcc
     4321 tgttccatta actgcgctaa aaataatttt taaatctttt ttagcttctt gctctttttt
     4381 gtacgtctct gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
     4441 ttgaaaaagt ggcaccgagt cggtgctttt ttgaattcga cattgattat tgactagtta
     4501 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     4561 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     4621 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     4681 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     4741 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     4801 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     4861 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     4921 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     4981 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     5041 ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt actggcttat
     5101 ctagagttaa cgcgctgcca ccatggacaa gaagtacagc atcggcctgg ccatcggcac
     5161 caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca agaaattcaa
     5221 ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag ccctgctgtt
     5281 cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa gaagatacac
     5341 cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga tggccaaggt
     5401 ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg ataagaagca
     5461 cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc
     5521 caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg acctgcggct
     5581 gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga tcgagggcga
     5641 cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc agacctacaa
     5701 ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc
     5761 tgccagactg agcaagagca gacggctgga aaatctgatc gcccagctgc ccggcgagaa
     5821 gaagaatggc ctgttcggaa acctgattgc cctgagcctg ggcctgaccc ccaacttcaa
     5881 gagcaacttc gacctggccg aggatgccaa actgcagctg agcaaggaca cctacgacga
     5941 cgacctggac aacctgctgg cccagatcgg cgaccagtac gccgacctgt ttctggccgc
     6001 caagaacctg tccgacgcca tcctgctgag cgacatcctg agagtgaaca ccgagatcac
     6061 caaggccccc ctgagcgcct ctatgatcaa gagatacgac gagcaccacc aggacctgac
     6121 cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag tacaaagaga ttttcttcga
     6181 ccagagcaag aacggctacg ccggctacat tgacggcgga gccagccagg aagagttcta
     6241 caagttcatc aagcccatcc tggaaaagat ggacggcacc gaggaactgc tcgtgaagct
     6301 gaacagagag gacctgctgc ggaagcagcg gaccttcgac aacggcagca tcccccacca
     6361 gatccacctg ggagagctgc acgccattct gcggcggcag gaagattttt acccattcct
     6421 gaaggacaac cgggaaaaga tcgagaagat cctgaccttc cgcatcccct actacgtggg
     6481 ccctctggcc aggggaaaca gcagattcgc ctggatgacc agaaagagcg aggaaaccat
     6541 caccccctgg aacttcgagg aagtggtgga caagggcgct tccgcccaga gcttcatcga
     6601 gcggatgacc aacttcgata agaacctgcc caacgagaag gtgctgccca agcacagcct
     6661 gctgtacgag tacttcaccg tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg
     6721 aatgagaaag cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt
     6781 caagaccaac cggaaagtga ccgtgaagca gctgaaagag gactacttca agaaaatcga
     6841 gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac
     6901 ataccacgat ctgctgaaaa ttatcaagga caaggacttc ctggacaatg aggaaaacga
     6961 ggacattctg gaagatatcg tgctgaccct gacactgttt gaggacagag agatgatcga
     7021 ggaacggctg aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg
     7081 gcggagatac accggctggg gcaggctgag ccggaagctg atcaacggca tccgggacaa
     7141 gcagtccggc aagacaatcc tggatttcct gaagtccgac ggcttcgcca acagaaactt
     7201 catgcagctg atccacgacg acagcctgac ctttaaagag gacatccaga aagcccaggt
     7261 gtccggccag ggcgatagcc tgcacgagca cattgccaat ctggccggca gccccgccat
     7321 taagaagggc atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg
     7381 gcacaagccc gagaacatcg tgatcgaaat ggccagagag aaccagacca cccagaaggg
     7441 acagaagaac agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag agctgggcag
     7501 ccagatcctg aaagaacacc ccgtggaaaa cacccagctg cagaacgaga agctgtacct
     7561 gtactacctg cagaatgggc gggatatgta cgtggaccag gaactggaca tcaaccggct
     7621 gtccgactac gatgtggacc atatcgtgcc tcagagcttt ctgaaggacg actccatcga
     7681 caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg tgccctccga
     7741 agaggtcgtg aagaagatga agaactactg gcggcagctg ctgaacgcca agctgattac
     7801 ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg aactggataa
     7861 ggccggcttc atcaagagac agctggtgga aacccggcag atcacaaagc acgtggcaca
     7921 gatcctggac tcccggatga acactaagta cgacgagaat gacaagctga tccgggaagt
     7981 gaaagtgatc accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta
     8041 caaagtgcgc gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt
     8101 gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta
     8161 caaggtgtac gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac
     8221 cgccaagtac ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc
     8281 caacggcgag atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt
     8341 gtgggataag ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa
     8401 tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa
     8461 gaggaacagc gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg
     8521 cttcgacagc cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa
     8581 gtccaagaaa ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag
     8641 cttcgagaag aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga
     8701 cctgatcatc aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat
     8761 gctggcctct gccggcgaac tgcagaaggg aaacgaactg gccctgccct ccaaatatgt
     8821 gaacttcctg tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga
     8881 gcagaaacag ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat
     8941 cagcgagttc tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc
     9001 ctacaacaag caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt
     9061 taccctgacc aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg
     9121 gaagaggtac accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac
     9181 cggcctgtac gagacacgga tcgacctgtc tcagctggga ggcgacaagc gacctgccgc
     9241 cacaaagaag gctggacagg ctaagaagaa gaaagattac aaagacgatg acgataaggg
     9301 atccggcgca acaaacttct ctctgctgaa acaagccgga gatgtcgaag agaatcctgg
     9361 accgatggcc cagtccaagc acggcctgac caaggagatg accatgaagt accgcatgga
     9421 gggctgcgtg gacggccaca agttcgtgat caccggcgag ggcatcggct accccttcaa
     9481 gggcaagcag gccatcaacc tgtgcgtggt ggagggcggc cccttgccct tcgccgagga
     9541 catcttgtcc gccgccttca tgtacggcaa ccgcgtgttc accgagtacc cccaggacat
     9601 cgtcgactac ttcaagaact cctgccccgc cggctacacc tgggaccgct ccttcctgtt
     9661 cgaggacggc gccgtgtgca tctgcaacgc cgacatcacc gtgagcgtgg aggagaactg
     9721 catgtaccac gagtccaagt tctacggcgt gaacttcccc gccgacggcc ccgtgatgaa
     9781 gaagatgacc gacaactggg agccctcctg cgagaagatc atccccgtgc ccaagcaggg
     9841 catcttgaag ggcgacgtga gcatgtacct gctgctgaag gacggtggcc gcttgcgctg
     9901 ccagttcgac accgtgtaca aggccaagtc cgtgccccgc aagatgcccg actggcactt
     9961 catccagcac aagctgaccc gcgaggaccg cagcgacgcc aagaaccaga agtggcacct
    10021 gaccgagcac gccatcgcct ccggctccgc cttgccctga acgcgtctgg aacaatcaac
    10081 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
    10141 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
    10201 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
    10261 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
    10321 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
    10381 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
    10441 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
    10501 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
    10561 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
    10621 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattctgcag
    10681 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
    10741 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt ttttccagtc acacctcagg
    10801 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
    10861 agaggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
    10921 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
    10981 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
    11041 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
    11101 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
    11161 cagagagtga gaggccttga cattgtttaa actggaatgt gtgtcagtta gggtgtggaa
    11221 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
    11281 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
    11341 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
    11401 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
    11461 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
    11521 tttgcaaaaa gctcccggga gcttgtatat ccattacatg tgagcaaaag gccagcaaaa
    11581 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    11641 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    11701 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    11761 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    11821 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    11881 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    11941 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    12001 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    12061 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    12121 ttgatccggc aaacaaacca ccgctggtag cggttttttt gtttgcaagc agcagattac
    12181 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    12241 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    12301 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    12361 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    12421 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    12481 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    12541 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    12601 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    12661 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    12721 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    12781 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    12841 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    12901 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    12961 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    13021 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    13081 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    13141 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    13201 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    13261 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    13321 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgt
//
