LOCUS       V012257                13038 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V012257
VERSION     V012257
KEYWORDS    pCLIP-All-Nickase(D10A)-TRE3G-Puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13038)
  AUTHORS   transOMIC
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 13038)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..404
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             411..668
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    715..840
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1333..1566
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1751..1795
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1944..1985
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2093..2210
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2261..2501
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    2506..4390
                     /label="Stuffer"
                     /note="Stuffer"
     misc_RNA        4391..4466
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     terminator      4467..4472
                     /note="polIII terminator"
                     /note="RNA polymerase III transcription terminator"
     promoter        4501..4868
                     /label="TRE3GS promoter"
                     /note="3rd-generation Tet-responsive promoter that can be
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription
                     factors"
     CDS             4892..8995
                     /label="Cas9(D10A)"
                     /note="nickase mutant of the Cas9 endonuclease from the
                     Streptococcus pyogenes Type II CRISPR/Cas system"
     CDS             8996..9043
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label="bipartite nuclear localization signal from nucl"
                     /note="nucleoplasmin NLS"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             9044..9067
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             9077..9133
                     /label="P2A"
                     /note="2A peptide from porcine teschovirus-1 polyprotein"
     CDS             9134..9727
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     CDS             complement(10215..10226)
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     LTR             10540..10773
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        10869..11198
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(11287..11872)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(12046..12903)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(12904..13008)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatcctcg cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accagctagc gaggcgtggc
      421 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
      481 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
      541 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
      601 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
      661 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
      721 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
      781 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
      841 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
      901 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
      961 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     1021 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     1081 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     1141 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     1201 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1261 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1321 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1381 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     1441 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     1501 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     1561 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     1621 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     1681 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     1741 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     1801 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     1861 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     1921 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     1981 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     2041 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     2101 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2161 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     2221 acagggacag cagagatcca gtttggttaa ttaaggtacc gagggcctat ttcccatgat
     2281 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac
     2341 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
     2401 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa
     2461 gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg gagacggttg
     2521 taaatgagca cacaaaatac acatgctaaa atattatatt ctatgacctt tataaaatca
     2581 accaaaatct tctttttaat aactttagta tcaataatta gaatttttat gttccttttt
     2641 gcaaactttt aataaaaatg agcaaaataa aaaaacgcta gttttagtaa ctcgcgttgt
     2701 tttcttcacc tttaataata gctactccac cacttgttcc taagcggtca gctcctgctt
     2761 caatcatttt ttgagcatct tcaaatgttc taactccacc agctgcttta actaaagcat
     2821 tgtctttaac aactgacttc attagtttaa catcttcaaa tgttgcacct gattttgaaa
     2881 atcctgttga tgttttaaca aattctaatc cagcttcaac agctatttca caagctttca
     2941 tgatttcttc ttttgttaat aaacaatttt ccataataca tttaacaaca tgtgatccag
     3001 ctgctttttt tacagctttc atgtcttcta aaactaattc ataatttttg tcttttaatg
     3061 caccaatatt taataccata tcaatttctg ttgcaccatc tttaattgct tcagaaactt
     3121 cgaatgcttt tgtagctgtt gtgcatgcac ctagaggaaa acctacaaca tttgttattc
     3181 ctacatttgt gccttttaat aattctttac aatagcttgt tcaatatgaa ttaacacaaa
     3241 ctgttgcaaa atcaaattca attgcttcat cacataattg tttaatttca gctttcgtag
     3301 catcttgttt taataatgtg tgatctatat atttgtttag tttcattttt tctcctatat
     3361 attcattttt aattttaatt ctttaataat ttcgtctact ttaactttag cgttttgaac
     3421 agattcacca acacctataa aataaatttt tagtttaggt tcagttccac ttgggcgaac
     3481 agcaaatcat gacttatctt ctaaataaaa ttttagtaag tcttgtcctg gcatattata
     3541 cattccatcg atgtagtctt caacattaac aactttaagt ccagcaattt gagttaaggg
     3601 tgttgctctc aatgatttca ttaatggttc aatttttaat ttcttttctt ctggtttaaa
     3661 attcaagttt aaagtgaaag tgtaatatgc acccatttct ttaaataaat cttctaaata
     3721 gtctactaat gttttatttt gttttttata aaatcaagca gcctctgcta ttaatataga
     3781 agcttgtatt ccatctttat ctctagctga gtcatcaatt acatatccat aactttcttc
     3841 ataagcaaaa acaaaattta atccgttatc ttcttcttta gcaatttctc tacccattca
     3901 tttaaatcca gttaaagttt ttacaatatt aactccatat ttttcatgag cgattctatc
     3961 acccaaatca cttgttacaa aacttgaata tagagccgga ttttttggaa tgctatttaa
     4021 gcgttttaga tttgataatt ttcaatcaat taaaattggt cctgtttgat ttccatctaa
     4081 tcttacaaaa tgaccatcat gttttattgc cattccaaat ctgtcagcat ctgggtcatt
     4141 cataataata atatctgcat catgtttaat accatattca agcggtattt ttcatgcagg
     4201 atcaaattct ggatttggat ttacaacatt tttaaatgtt tcatcttcaa atgcatgctc
     4261 ttcaacctca ataacgttat atcctgattc acgtaatatt tttggggtaa atttagttcc
     4321 tgttccatta actgcgctaa aaataatttt taaatctttt ttagcttctt gctctttttt
     4381 gtacgtctct gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
     4441 ttgaaaaagt ggcaccgagt cggtgctttt ttgaattcaa ccccgtggaa ttatcacctc
     4501 gagtttactc cctatcagtg atagagaacg tatgaagagt ttactcccta tcagtgatag
     4561 agaacgtatg cagactttac tccctatcag tgatagagaa cgtataagga gtttactccc
     4621 tatcagtgat agagaacgta tgaccagttt actccctatc agtgatagag aacgtatcta
     4681 cagtttactc cctatcagtg atagagaacg tatatccagt ttactcccta tcagtgatag
     4741 agaacgtata agctttgctt atgtaaacca gggcgcctat aaaagagtgc tgattttttg
     4801 agtaaacttc aattccacaa cacttttgtc ttataccaac tttccgtacc acttcctacc
     4861 ctcgtaaatc tagagttaac gcgctgccac catggacaag aagtacagca tcggcctggc
     4921 catcggcacc aactctgtgg gctgggccgt gatcaccgac gagtacaagg tgcccagcaa
     4981 gaaattcaag gtgctgggca acaccgaccg gcacagcatc aagaagaacc tgatcggagc
     5041 cctgctgttc gacagcggcg aaacagccga ggccacccgg ctgaagagaa ccgccagaag
     5101 aagatacacc agacggaaga accggatctg ctatctgcaa gagatcttca gcaacgagat
     5161 ggccaaggtg gacgacagct tcttccacag actggaagag tccttcctgg tggaagagga
     5221 taagaagcac gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg cctaccacga
     5281 gaagtacccc accatctacc acctgagaaa gaaactggtg gacagcaccg acaaggccga
     5341 cctgcggctg atctatctgg ccctggccca catgatcaag ttccggggcc acttcctgat
     5401 cgagggcgac ctgaaccccg acaacagcga cgtggacaag ctgttcatcc agctggtgca
     5461 gacctacaac cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc
     5521 catcctgtct gccagactga gcaagagcag acggctggaa aatctgatcg cccagctgcc
     5581 cggcgagaag aagaatggcc tgttcggaaa cctgattgcc ctgagcctgg gcctgacccc
     5641 caacttcaag agcaacttcg acctggccga ggatgccaaa ctgcagctga gcaaggacac
     5701 ctacgacgac gacctggaca acctgctggc ccagatcggc gaccagtacg ccgacctgtt
     5761 tctggccgcc aagaacctgt ccgacgccat cctgctgagc gacatcctga gagtgaacac
     5821 cgagatcacc aaggcccccc tgagcgcctc tatgatcaag agatacgacg agcaccacca
     5881 ggacctgacc ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt acaaagagat
     5941 tttcttcgac cagagcaaga acggctacgc cggctacatt gacggcggag ccagccagga
     6001 agagttctac aagttcatca agcccatcct ggaaaagatg gacggcaccg aggaactgct
     6061 cgtgaagctg aacagagagg acctgctgcg gaagcagcgg accttcgaca acggcagcat
     6121 cccccaccag atccacctgg gagagctgca cgccattctg cggcggcagg aagattttta
     6181 cccattcctg aaggacaacc gggaaaagat cgagaagatc ctgaccttcc gcatccccta
     6241 ctacgtgggc cctctggcca ggggaaacag cagattcgcc tggatgacca gaaagagcga
     6301 ggaaaccatc accccctgga acttcgagga agtggtggac aagggcgctt ccgcccagag
     6361 cttcatcgag cggatgacca acttcgataa gaacctgccc aacgagaagg tgctgcccaa
     6421 gcacagcctg ctgtacgagt acttcaccgt gtataacgag ctgaccaaag tgaaatacgt
     6481 gaccgaggga atgagaaagc ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga
     6541 cctgctgttc aagaccaacc ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa
     6601 gaaaatcgag tgcttcgact ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc
     6661 cctgggcaca taccacgatc tgctgaaaat tatcaaggac aaggacttcc tggacaatga
     6721 ggaaaacgag gacattctgg aagatatcgt gctgaccctg acactgtttg aggacagaga
     6781 gatgatcgag gaacggctga aaacctatgc ccacctgttc gacgacaaag tgatgaagca
     6841 gctgaagcgg cggagataca ccggctgggg caggctgagc cggaagctga tcaacggcat
     6901 ccgggacaag cagtccggca agacaatcct ggatttcctg aagtccgacg gcttcgccaa
     6961 cagaaacttc atgcagctga tccacgacga cagcctgacc tttaaagagg acatccagaa
     7021 agcccaggtg tccggccagg gcgatagcct gcacgagcac attgccaatc tggccggcag
     7081 ccccgccatt aagaagggca tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt
     7141 gatgggccgg cacaagcccg agaacatcgt gatcgaaatg gccagagaga accagaccac
     7201 ccagaaggga cagaagaaca gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga
     7261 gctgggcagc cagatcctga aagaacaccc cgtggaaaac acccagctgc agaacgagaa
     7321 gctgtacctg tactacctgc agaatgggcg ggatatgtac gtggaccagg aactggacat
     7381 caaccggctg tccgactacg atgtggacca tatcgtgcct cagagctttc tgaaggacga
     7441 ctccatcgac aacaaggtgc tgaccagaag cgacaagaac cggggcaaga gcgacaacgt
     7501 gccctccgaa gaggtcgtga agaagatgaa gaactactgg cggcagctgc tgaacgccaa
     7561 gctgattacc cagagaaagt tcgacaatct gaccaaggcc gagagaggcg gcctgagcga
     7621 actggataag gccggcttca tcaagagaca gctggtggaa acccggcaga tcacaaagca
     7681 cgtggcacag atcctggact cccggatgaa cactaagtac gacgagaatg acaagctgat
     7741 ccgggaagtg aaagtgatca ccctgaagtc caagctggtg tccgatttcc ggaaggattt
     7801 ccagttttac aaagtgcgcg agatcaacaa ctaccaccac gcccacgacg cctacctgaa
     7861 cgccgtcgtg ggaaccgccc tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta
     7921 cggcgactac aaggtgtacg acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg
     7981 caaggctacc gccaagtact tcttctacag caacatcatg aactttttca agaccgagat
     8041 taccctggcc aacggcgaga tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg
     8101 ggagatcgtg tgggataagg gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc
     8161 ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca aagagtctat
     8221 cctgcccaag aggaacagcg ataagctgat cgccagaaag aaggactggg accctaagaa
     8281 gtacggcggc ttcgacagcc ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga
     8341 aaagggcaag tccaagaaac tgaagagtgt gaaagagctg ctggggatca ccatcatgga
     8401 aagaagcagc ttcgagaaga atcccatcga ctttctggaa gccaagggct acaaagaagt
     8461 gaaaaaggac ctgatcatca agctgcctaa gtactccctg ttcgagctgg aaaacggccg
     8521 gaagagaatg ctggcctctg ccggcgaact gcagaaggga aacgaactgg ccctgccctc
     8581 caaatatgtg aacttcctgt acctggccag ccactatgag aagctgaagg gctcccccga
     8641 ggataatgag cagaaacagc tgtttgtgga acagcacaag cactacctgg acgagatcat
     8701 cgagcagatc agcgagttct ccaagagagt gatcctggcc gacgctaatc tggacaaagt
     8761 gctgtccgcc tacaacaagc accgggataa gcccatcaga gagcaggccg agaatatcat
     8821 ccacctgttt accctgacca atctgggagc ccctgccgcc ttcaagtact ttgacaccac
     8881 catcgaccgg aagaggtaca ccagcaccaa agaggtgctg gacgccaccc tgatccacca
     8941 gagcatcacc ggcctgtacg agacacggat cgacctgtct cagctgggag gcgacaagcg
     9001 acctgccgcc acaaagaagg ctggacaggc taagaagaag aaagattaca aagacgatga
     9061 cgataaggga tccggcgcaa caaacttctc tctgctgaaa caagccggag atgtcgaaga
     9121 gaatcctgga ccgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     9181 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     9241 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     9301 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     9361 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     9421 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     9481 gcccaaggag cccgcgtggt tcctggccac cgtcggagtc tcgcccgacc accagggcaa
     9541 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     9601 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     9661 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     9721 cggtgcctga acgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga
     9781 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     9841 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     9901 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     9961 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
    10021 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
    10081 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
    10141 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
    10201 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
    10261 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
    10321 ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac
    10381 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
    10441 ggaggtgggt ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
    10501 ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc
    10561 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
    10621 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
    10681 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
    10741 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
    10801 agtatttata acttgcaaag aaatgaatat cagagagtga gaggccttga cattgtttaa
    10861 actggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
    10921 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
    10981 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
    11041 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
    11101 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
    11161 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
    11221 ccattacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    11281 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    11341 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    11401 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    11461 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    11521 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    11581 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    11641 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    11701 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
    11761 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    11821 cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    11881 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    11941 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    12001 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    12061 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    12121 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    12181 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    12241 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    12301 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    12361 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    12421 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    12481 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    12541 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    12601 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    12661 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    12721 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    12781 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    12841 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    12901 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    12961 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    13021 aaaagtgcca cctgacgt
//
