LOCUS       V012262                13015 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V012262
VERSION     V012262
KEYWORDS    pCLIP-All-Nickase(D10A)-EFS-ZsGreen
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13015)
  AUTHORS   transOMIC
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 13015)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13015
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..404
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             411..668
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    715..840
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1333..1566
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1751..1795
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1944..1985
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2093..2210
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2261..2501
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    2506..4390
                     /label="Stuffer"
                     /note="Stuffer"
     misc_RNA        4391..4466
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     terminator      4467..4472
                     /note="polIII terminator"
                     /note="RNA polymerase III transcription terminator"
     promoter        4528..4739
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     CDS             4770..8873
                     /label="Cas9(D10A)"
                     /note="nickase mutant of the Cas9 endonuclease from the
                     Streptococcus pyogenes Type II CRISPR/Cas system"
     CDS             8874..8921
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label="bipartite nuclear localization signal from nucl"
                     /note="nucleoplasmin NLS"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8922..8945
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             8955..9011
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label="2A peptide from porcine teschovirus-1 polyprotein"
                     /note="P2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9012..9704
                     /label="ZsGreen1"
                     /note="Zoanthus green fluorescent protein"
     CDS             complement(10192..10203)
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     LTR             10517..10750
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        10846..11175
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(11264..11849)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(12023..12880)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(12881..12985)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatcctcg cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accagctagc gaggcgtggc
      421 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
      481 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
      541 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
      601 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
      661 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
      721 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
      781 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
      841 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
      901 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
      961 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     1021 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     1081 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     1141 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     1201 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1261 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1321 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1381 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     1441 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     1501 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     1561 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     1621 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     1681 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     1741 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     1801 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     1861 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     1921 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     1981 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     2041 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     2101 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2161 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     2221 acagggacag cagagatcca gtttggttaa ttaaggtacc gagggcctat ttcccatgat
     2281 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac
     2341 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
     2401 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa
     2461 gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg gagacggttg
     2521 taaatgagca cacaaaatac acatgctaaa atattatatt ctatgacctt tataaaatca
     2581 accaaaatct tctttttaat aactttagta tcaataatta gaatttttat gttccttttt
     2641 gcaaactttt aataaaaatg agcaaaataa aaaaacgcta gttttagtaa ctcgcgttgt
     2701 tttcttcacc tttaataata gctactccac cacttgttcc taagcggtca gctcctgctt
     2761 caatcatttt ttgagcatct tcaaatgttc taactccacc agctgcttta actaaagcat
     2821 tgtctttaac aactgacttc attagtttaa catcttcaaa tgttgcacct gattttgaaa
     2881 atcctgttga tgttttaaca aattctaatc cagcttcaac agctatttca caagctttca
     2941 tgatttcttc ttttgttaat aaacaatttt ccataataca tttaacaaca tgtgatccag
     3001 ctgctttttt tacagctttc atgtcttcta aaactaattc ataatttttg tcttttaatg
     3061 caccaatatt taataccata tcaatttctg ttgcaccatc tttaattgct tcagaaactt
     3121 cgaatgcttt tgtagctgtt gtgcatgcac ctagaggaaa acctacaaca tttgttattc
     3181 ctacatttgt gccttttaat aattctttac aatagcttgt tcaatatgaa ttaacacaaa
     3241 ctgttgcaaa atcaaattca attgcttcat cacataattg tttaatttca gctttcgtag
     3301 catcttgttt taataatgtg tgatctatat atttgtttag tttcattttt tctcctatat
     3361 attcattttt aattttaatt ctttaataat ttcgtctact ttaactttag cgttttgaac
     3421 agattcacca acacctataa aataaatttt tagtttaggt tcagttccac ttgggcgaac
     3481 agcaaatcat gacttatctt ctaaataaaa ttttagtaag tcttgtcctg gcatattata
     3541 cattccatcg atgtagtctt caacattaac aactttaagt ccagcaattt gagttaaggg
     3601 tgttgctctc aatgatttca ttaatggttc aatttttaat ttcttttctt ctggtttaaa
     3661 attcaagttt aaagtgaaag tgtaatatgc acccatttct ttaaataaat cttctaaata
     3721 gtctactaat gttttatttt gttttttata aaatcaagca gcctctgcta ttaatataga
     3781 agcttgtatt ccatctttat ctctagctga gtcatcaatt acatatccat aactttcttc
     3841 ataagcaaaa acaaaattta atccgttatc ttcttcttta gcaatttctc tacccattca
     3901 tttaaatcca gttaaagttt ttacaatatt aactccatat ttttcatgag cgattctatc
     3961 acccaaatca cttgttacaa aacttgaata tagagccgga ttttttggaa tgctatttaa
     4021 gcgttttaga tttgataatt ttcaatcaat taaaattggt cctgtttgat ttccatctaa
     4081 tcttacaaaa tgaccatcat gttttattgc cattccaaat ctgtcagcat ctgggtcatt
     4141 cataataata atatctgcat catgtttaat accatattca agcggtattt ttcatgcagg
     4201 atcaaattct ggatttggat ttacaacatt tttaaatgtt tcatcttcaa atgcatgctc
     4261 ttcaacctca ataacgttat atcctgattc acgtaatatt tttggggtaa atttagttcc
     4321 tgttccatta actgcgctaa aaataatttt taaatctttt ttagcttctt gctctttttt
     4381 gtacgtctct gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
     4441 ttgaaaaagt ggcaccgagt cggtgctttt ttgaattcgc tagctaggtc ttgaaaggag
     4501 tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     4561 agttgggggg aggggtcggc aattgatccg gtgcctagag aaggtggcgc ggggtaaact
     4621 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     4681 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
     4741 accggttcta gagttaacgc gctgccacca tggacaagaa gtacagcatc ggcctggcca
     4801 tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg cccagcaaga
     4861 aattcaaggt gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggagccc
     4921 tgctgttcga cagcggcgaa acagccgagg ccacccggct gaagagaacc gccagaagaa
     4981 gatacaccag acggaagaac cggatctgct atctgcaaga gatcttcagc aacgagatgg
     5041 ccaaggtgga cgacagcttc ttccacagac tggaagagtc cttcctggtg gaagaggata
     5101 agaagcacga gcggcacccc atcttcggca acatcgtgga cgaggtggcc taccacgaga
     5161 agtaccccac catctaccac ctgagaaaga aactggtgga cagcaccgac aaggccgacc
     5221 tgcggctgat ctatctggcc ctggcccaca tgatcaagtt ccggggccac ttcctgatcg
     5281 agggcgacct gaaccccgac aacagcgacg tggacaagct gttcatccag ctggtgcaga
     5341 cctacaacca gctgttcgag gaaaacccca tcaacgccag cggcgtggac gccaaggcca
     5401 tcctgtctgc cagactgagc aagagcagac ggctggaaaa tctgatcgcc cagctgcccg
     5461 gcgagaagaa gaatggcctg ttcggaaacc tgattgccct gagcctgggc ctgaccccca
     5521 acttcaagag caacttcgac ctggccgagg atgccaaact gcagctgagc aaggacacct
     5581 acgacgacga cctggacaac ctgctggccc agatcggcga ccagtacgcc gacctgtttc
     5641 tggccgccaa gaacctgtcc gacgccatcc tgctgagcga catcctgaga gtgaacaccg
     5701 agatcaccaa ggcccccctg agcgcctcta tgatcaagag atacgacgag caccaccagg
     5761 acctgaccct gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac aaagagattt
     5821 tcttcgacca gagcaagaac ggctacgccg gctacattga cggcggagcc agccaggaag
     5881 agttctacaa gttcatcaag cccatcctgg aaaagatgga cggcaccgag gaactgctcg
     5941 tgaagctgaa cagagaggac ctgctgcgga agcagcggac cttcgacaac ggcagcatcc
     6001 cccaccagat ccacctggga gagctgcacg ccattctgcg gcggcaggaa gatttttacc
     6061 cattcctgaa ggacaaccgg gaaaagatcg agaagatcct gaccttccgc atcccctact
     6121 acgtgggccc tctggccagg ggaaacagca gattcgcctg gatgaccaga aagagcgagg
     6181 aaaccatcac cccctggaac ttcgaggaag tggtggacaa gggcgcttcc gcccagagct
     6241 tcatcgagcg gatgaccaac ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc
     6301 acagcctgct gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga
     6361 ccgagggaat gagaaagccc gccttcctga gcggcgagca gaaaaaggcc atcgtggacc
     6421 tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac tacttcaaga
     6481 aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga agatcggttc aacgcctccc
     6541 tgggcacata ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg
     6601 aaaacgagga cattctggaa gatatcgtgc tgaccctgac actgtttgag gacagagaga
     6661 tgatcgagga acggctgaaa acctatgccc acctgttcga cgacaaagtg atgaagcagc
     6721 tgaagcggcg gagatacacc ggctggggca ggctgagccg gaagctgatc aacggcatcc
     6781 gggacaagca gtccggcaag acaatcctgg atttcctgaa gtccgacggc ttcgccaaca
     6841 gaaacttcat gcagctgatc cacgacgaca gcctgacctt taaagaggac atccagaaag
     6901 cccaggtgtc cggccagggc gatagcctgc acgagcacat tgccaatctg gccggcagcc
     6961 ccgccattaa gaagggcatc ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga
     7021 tgggccggca caagcccgag aacatcgtga tcgaaatggc cagagagaac cagaccaccc
     7081 agaagggaca gaagaacagc cgcgagagaa tgaagcggat cgaagagggc atcaaagagc
     7141 tgggcagcca gatcctgaaa gaacaccccg tggaaaacac ccagctgcag aacgagaagc
     7201 tgtacctgta ctacctgcag aatgggcggg atatgtacgt ggaccaggaa ctggacatca
     7261 accggctgtc cgactacgat gtggaccata tcgtgcctca gagctttctg aaggacgact
     7321 ccatcgacaa caaggtgctg accagaagcg acaagaaccg gggcaagagc gacaacgtgc
     7381 cctccgaaga ggtcgtgaag aagatgaaga actactggcg gcagctgctg aacgccaagc
     7441 tgattaccca gagaaagttc gacaatctga ccaaggccga gagaggcggc ctgagcgaac
     7501 tggataaggc cggcttcatc aagagacagc tggtggaaac ccggcagatc acaaagcacg
     7561 tggcacagat cctggactcc cggatgaaca ctaagtacga cgagaatgac aagctgatcc
     7621 gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc cgatttccgg aaggatttcc
     7681 agttttacaa agtgcgcgag atcaacaact accaccacgc ccacgacgcc tacctgaacg
     7741 ccgtcgtggg aaccgccctg atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg
     7801 gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca
     7861 aggctaccgc caagtacttc ttctacagca acatcatgaa ctttttcaag accgagatta
     7921 ccctggccaa cggcgagatc cggaagcggc ctctgatcga gacaaacggc gaaaccgggg
     7981 agatcgtgtg ggataagggc cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc
     8041 aagtgaatat cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa gagtctatcc
     8101 tgcccaagag gaacagcgat aagctgatcg ccagaaagaa ggactgggac cctaagaagt
     8161 acggcggctt cgacagcccc accgtggcct attctgtgct ggtggtggcc aaagtggaaa
     8221 agggcaagtc caagaaactg aagagtgtga aagagctgct ggggatcacc atcatggaaa
     8281 gaagcagctt cgagaagaat cccatcgact ttctggaagc caagggctac aaagaagtga
     8341 aaaaggacct gatcatcaag ctgcctaagt actccctgtt cgagctggaa aacggccgga
     8401 agagaatgct ggcctctgcc ggcgaactgc agaagggaaa cgaactggcc ctgccctcca
     8461 aatatgtgaa cttcctgtac ctggccagcc actatgagaa gctgaagggc tcccccgagg
     8521 ataatgagca gaaacagctg tttgtggaac agcacaagca ctacctggac gagatcatcg
     8581 agcagatcag cgagttctcc aagagagtga tcctggccga cgctaatctg gacaaagtgc
     8641 tgtccgccta caacaagcac cgggataagc ccatcagaga gcaggccgag aatatcatcc
     8701 acctgtttac cctgaccaat ctgggagccc ctgccgcctt caagtacttt gacaccacca
     8761 tcgaccggaa gaggtacacc agcaccaaag aggtgctgga cgccaccctg atccaccaga
     8821 gcatcaccgg cctgtacgag acacggatcg acctgtctca gctgggaggc gacaagcgac
     8881 ctgccgccac aaagaaggct ggacaggcta agaagaagaa agattacaaa gacgatgacg
     8941 ataagggatc cggcgcaaca aacttctctc tgctgaaaca agccggagat gtcgaagaga
     9001 atcctggacc gatggcccag tccaagcacg gcctgaccaa ggagatgacc atgaagtacc
     9061 gcatggaggg ctgcgtggac ggccacaagt tcgtgatcac cggcgagggc atcggctacc
     9121 ccttcaaggg caagcaggcc atcaacctgt gcgtggtgga gggcggcccc ttgcccttcg
     9181 ccgaggacat cttgtccgcc gccttcatgt acggcaaccg cgtgttcacc gagtaccccc
     9241 aggacatcgt cgactacttc aagaactcct gccccgccgg ctacacctgg gaccgctcct
     9301 tcctgttcga ggacggcgcc gtgtgcatct gcaacgccga catcaccgtg agcgtggagg
     9361 agaactgcat gtaccacgag tccaagttct acggcgtgaa cttccccgcc gacggccccg
     9421 tgatgaagaa gatgaccgac aactgggagc cctcctgcga gaagatcatc cccgtgccca
     9481 agcagggcat cttgaagggc gacgtgagca tgtacctgct gctgaaggac ggtggccgct
     9541 tgcgctgcca gttcgacacc gtgtacaagg ccaagtccgt gccccgcaag atgcccgact
     9601 ggcacttcat ccagcacaag ctgacccgcg aggaccgcag cgacgccaag aaccagaagt
     9661 ggcacctgac cgagcacgcc atcgcctccg gctccgcctt gccctgaacg cgtctggaac
     9721 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     9781 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     9841 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     9901 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     9961 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
    10021 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
    10081 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
    10141 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
    10201 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
    10261 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat
    10321 tctgcagtcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
    10381 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt tccagtcaca
    10441 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
    10501 aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
    10561 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
    10621 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
    10681 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
    10741 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
    10801 tgaatatcag agagtgagag gccttgacat tgtttaaact ggaatgtgtg tcagttaggg
    10861 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
    10921 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
    10981 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
    11041 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
    11101 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
    11161 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttacatgtga gcaaaaggcc
    11221 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    11281 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    11341 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    11401 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    11461 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    11521 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    11581 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    11641 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    11701 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    11761 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc
    11821 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    11881 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    11941 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    12001 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    12061 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    12121 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    12181 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    12241 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    12301 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    12361 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    12421 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    12481 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    12541 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    12601 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    12661 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    12721 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    12781 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    12841 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    12901 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    12961 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgt
//
