LOCUS       V012271                13008 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V012271
VERSION     V012271
KEYWORDS    pCLIP-All-EFS-tRFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13008)
  AUTHORS   transOMIC
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 13008)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..404
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             411..668
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    715..840
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1333..1566
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1751..1795
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1944..1985
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2093..2210
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2261..2501
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    2506..4390
                     /label="Stuffer"
                     /note="Stuffer"
     misc_RNA        4391..4466
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     terminator      4467..4472
                     /note="polIII terminator"
                     /note="RNA polymerase III transcription terminator"
     promoter        4528..4739
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     CDS             4764..8867
                     /label="Cas9"
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
     CDS             8868..8915
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label="bipartite nuclear localization signal from nucl"
                     /note="nucleoplasmin NLS"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8916..8939
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             8949..9005
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label="2A peptide from porcine teschovirus-1 polyprotein"
                     /note="P2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9006..9698
                     /label="TurboRFP"
                     /note="red fluorescent protein from Entacmaea quadricolor"
     CDS             complement(10185..10196)
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     LTR             10510..10743
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        10839..11168
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(11257..11842)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(12016..12873)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(12874..12978)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatcctcg cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accagctagc gaggcgtggc
      421 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
      481 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
      541 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
      601 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
      661 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
      721 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
      781 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
      841 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
      901 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
      961 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     1021 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     1081 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     1141 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     1201 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1261 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1321 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1381 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     1441 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     1501 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     1561 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     1621 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     1681 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     1741 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     1801 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     1861 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     1921 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     1981 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     2041 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     2101 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2161 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     2221 acagggacag cagagatcca gtttggttaa ttaaggtacc gagggcctat ttcccatgat
     2281 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac
     2341 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
     2401 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa
     2461 gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg gagacggttg
     2521 taaatgagca cacaaaatac acatgctaaa atattatatt ctatgacctt tataaaatca
     2581 accaaaatct tctttttaat aactttagta tcaataatta gaatttttat gttccttttt
     2641 gcaaactttt aataaaaatg agcaaaataa aaaaacgcta gttttagtaa ctcgcgttgt
     2701 tttcttcacc tttaataata gctactccac cacttgttcc taagcggtca gctcctgctt
     2761 caatcatttt ttgagcatct tcaaatgttc taactccacc agctgcttta actaaagcat
     2821 tgtctttaac aactgacttc attagtttaa catcttcaaa tgttgcacct gattttgaaa
     2881 atcctgttga tgttttaaca aattctaatc cagcttcaac agctatttca caagctttca
     2941 tgatttcttc ttttgttaat aaacaatttt ccataataca tttaacaaca tgtgatccag
     3001 ctgctttttt tacagctttc atgtcttcta aaactaattc ataatttttg tcttttaatg
     3061 caccaatatt taataccata tcaatttctg ttgcaccatc tttaattgct tcagaaactt
     3121 cgaatgcttt tgtagctgtt gtgcatgcac ctagaggaaa acctacaaca tttgttattc
     3181 ctacatttgt gccttttaat aattctttac aatagcttgt tcaatatgaa ttaacacaaa
     3241 ctgttgcaaa atcaaattca attgcttcat cacataattg tttaatttca gctttcgtag
     3301 catcttgttt taataatgtg tgatctatat atttgtttag tttcattttt tctcctatat
     3361 attcattttt aattttaatt ctttaataat ttcgtctact ttaactttag cgttttgaac
     3421 agattcacca acacctataa aataaatttt tagtttaggt tcagttccac ttgggcgaac
     3481 agcaaatcat gacttatctt ctaaataaaa ttttagtaag tcttgtcctg gcatattata
     3541 cattccatcg atgtagtctt caacattaac aactttaagt ccagcaattt gagttaaggg
     3601 tgttgctctc aatgatttca ttaatggttc aatttttaat ttcttttctt ctggtttaaa
     3661 attcaagttt aaagtgaaag tgtaatatgc acccatttct ttaaataaat cttctaaata
     3721 gtctactaat gttttatttt gttttttata aaatcaagca gcctctgcta ttaatataga
     3781 agcttgtatt ccatctttat ctctagctga gtcatcaatt acatatccat aactttcttc
     3841 ataagcaaaa acaaaattta atccgttatc ttcttcttta gcaatttctc tacccattca
     3901 tttaaatcca gttaaagttt ttacaatatt aactccatat ttttcatgag cgattctatc
     3961 acccaaatca cttgttacaa aacttgaata tagagccgga ttttttggaa tgctatttaa
     4021 gcgttttaga tttgataatt ttcaatcaat taaaattggt cctgtttgat ttccatctaa
     4081 tcttacaaaa tgaccatcat gttttattgc cattccaaat ctgtcagcat ctgggtcatt
     4141 cataataata atatctgcat catgtttaat accatattca agcggtattt ttcatgcagg
     4201 atcaaattct ggatttggat ttacaacatt tttaaatgtt tcatcttcaa atgcatgctc
     4261 ttcaacctca ataacgttat atcctgattc acgtaatatt tttggggtaa atttagttcc
     4321 tgttccatta actgcgctaa aaataatttt taaatctttt ttagcttctt gctctttttt
     4381 gtacgtctct gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
     4441 ttgaaaaagt ggcaccgagt cggtgctttt ttgaattcgc tagctaggtc ttgaaaggag
     4501 tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     4561 agttgggggg aggggtcggc aattgatccg gtgcctagag aaggtggcgc ggggtaaact
     4621 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     4681 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
     4741 accggttcta gagcgctgcc accatggaca agaagtacag catcggcctg gacatcggca
     4801 ccaactctgt gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc aagaaattca
     4861 aggtgctggg caacaccgac cggcacagca tcaagaagaa cctgatcgga gccctgctgt
     4921 tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag aaccgccaga agaagataca
     4981 ccagacggaa gaaccggatc tgctatctgc aagagatctt cagcaacgag atggccaagg
     5041 tggacgacag cttcttccac agactggaag agtccttcct ggtggaagag gataagaagc
     5101 acgagcggca ccccatcttc ggcaacatcg tggacgaggt ggcctaccac gagaagtacc
     5161 ccaccatcta ccacctgaga aagaaactgg tggacagcac cgacaaggcc gacctgcggc
     5221 tgatctatct ggccctggcc cacatgatca agttccgggg ccacttcctg atcgagggcg
     5281 acctgaaccc cgacaacagc gacgtggaca agctgttcat ccagctggtg cagacctaca
     5341 accagctgtt cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag gccatcctgt
     5401 ctgccagact gagcaagagc agacggctgg aaaatctgat cgcccagctg cccggcgaga
     5461 agaagaatgg cctgttcgga aacctgattg ccctgagcct gggcctgacc cccaacttca
     5521 agagcaactt cgacctggcc gaggatgcca aactgcagct gagcaaggac acctacgacg
     5581 acgacctgga caacctgctg gcccagatcg gcgaccagta cgccgacctg tttctggccg
     5641 ccaagaacct gtccgacgcc atcctgctga gcgacatcct gagagtgaac accgagatca
     5701 ccaaggcccc cctgagcgcc tctatgatca agagatacga cgagcaccac caggacctga
     5761 ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa gtacaaagag attttcttcg
     5821 accagagcaa gaacggctac gccggctaca ttgacggcgg agccagccag gaagagttct
     5881 acaagttcat caagcccatc ctggaaaaga tggacggcac cgaggaactg ctcgtgaagc
     5941 tgaacagaga ggacctgctg cggaagcagc ggaccttcga caacggcagc atcccccacc
     6001 agatccacct gggagagctg cacgccattc tgcggcggca ggaagatttt tacccattcc
     6061 tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc tactacgtgg
     6121 gccctctggc caggggaaac agcagattcg cctggatgac cagaaagagc gaggaaacca
     6181 tcaccccctg gaacttcgag gaagtggtgg acaagggcgc ttccgcccag agcttcatcg
     6241 agcggatgac caacttcgat aagaacctgc ccaacgagaa ggtgctgccc aagcacagcc
     6301 tgctgtacga gtacttcacc gtgtataacg agctgaccaa agtgaaatac gtgaccgagg
     6361 gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg gacctgctgt
     6421 tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga ggactacttc aagaaaatcg
     6481 agtgcttcga ctccgtggaa atctccggcg tggaagatcg gttcaacgcc tccctgggca
     6541 cataccacga tctgctgaaa attatcaagg acaaggactt cctggacaat gaggaaaacg
     6601 aggacattct ggaagatatc gtgctgaccc tgacactgtt tgaggacaga gagatgatcg
     6661 aggaacggct gaaaacctat gcccacctgt tcgacgacaa agtgatgaag cagctgaagc
     6721 ggcggagata caccggctgg ggcaggctga gccggaagct gatcaacggc atccgggaca
     6781 agcagtccgg caagacaatc ctggatttcc tgaagtccga cggcttcgcc aacagaaact
     6841 tcatgcagct gatccacgac gacagcctga cctttaaaga ggacatccag aaagcccagg
     6901 tgtccggcca gggcgatagc ctgcacgagc acattgccaa tctggccggc agccccgcca
     6961 ttaagaaggg catcctgcag acagtgaagg tggtggacga gctcgtgaaa gtgatgggcc
     7021 ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga gaaccagacc acccagaagg
     7081 gacagaagaa cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa gagctgggca
     7141 gccagatcct gaaagaacac cccgtggaaa acacccagct gcagaacgag aagctgtacc
     7201 tgtactacct gcagaatggg cgggatatgt acgtggacca ggaactggac atcaaccggc
     7261 tgtccgacta cgatgtggac catatcgtgc ctcagagctt tctgaaggac gactccatcg
     7321 acaacaaggt gctgaccaga agcgacaaga accggggcaa gagcgacaac gtgccctccg
     7381 aagaggtcgt gaagaagatg aagaactact ggcggcagct gctgaacgcc aagctgatta
     7441 cccagagaaa gttcgacaat ctgaccaagg ccgagagagg cggcctgagc gaactggata
     7501 aggccggctt catcaagaga cagctggtgg aaacccggca gatcacaaag cacgtggcac
     7561 agatcctgga ctcccggatg aacactaagt acgacgagaa tgacaagctg atccgggaag
     7621 tgaaagtgat caccctgaag tccaagctgg tgtccgattt ccggaaggat ttccagtttt
     7681 acaaagtgcg cgagatcaac aactaccacc acgcccacga cgcctacctg aacgccgtcg
     7741 tgggaaccgc cctgatcaaa aagtacccta agctggaaag cgagttcgtg tacggcgact
     7801 acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta
     7861 ccgccaagta cttcttctac agcaacatca tgaacttttt caagaccgag attaccctgg
     7921 ccaacggcga gatccggaag cggcctctga tcgagacaaa cggcgaaacc ggggagatcg
     7981 tgtgggataa gggccgggat tttgccaccg tgcggaaagt gctgagcatg ccccaagtga
     8041 atatcgtgaa aaagaccgag gtgcagacag gcggcttcag caaagagtct atcctgccca
     8101 agaggaacag cgataagctg atcgccagaa agaaggactg ggaccctaag aagtacggcg
     8161 gcttcgacag ccccaccgtg gcctattctg tgctggtggt ggccaaagtg gaaaagggca
     8221 agtccaagaa actgaagagt gtgaaagagc tgctggggat caccatcatg gaaagaagca
     8281 gcttcgagaa gaatcccatc gactttctgg aagccaaggg ctacaaagaa gtgaaaaagg
     8341 acctgatcat caagctgcct aagtactccc tgttcgagct ggaaaacggc cggaagagaa
     8401 tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc tccaaatatg
     8461 tgaacttcct gtacctggcc agccactatg agaagctgaa gggctccccc gaggataatg
     8521 agcagaaaca gctgtttgtg gaacagcaca agcactacct ggacgagatc atcgagcaga
     8581 tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa gtgctgtccg
     8641 cctacaacaa gcaccgggat aagcccatca gagagcaggc cgagaatatc atccacctgt
     8701 ttaccctgac caatctggga gcccctgccg ccttcaagta ctttgacacc accatcgacc
     8761 ggaagaggta caccagcacc aaagaggtgc tggacgccac cctgatccac cagagcatca
     8821 ccggcctgta cgagacacgg atcgacctgt ctcagctggg aggcgacaag cgacctgccg
     8881 ccacaaagaa ggctggacag gctaagaaga agaaagatta caaagacgat gacgataagg
     8941 gatccggcgc aacaaacttc tctctgctga aacaagccgg agatgtcgaa gagaatcctg
     9001 gaccgatgag cgagctgatc aaggagaaca tgcacatgaa gctgtacatg gagggcaccg
     9061 tgaacaacca ccacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc
     9121 agaccatgaa gatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg
     9181 ctaccagctt catgtacggc agcaaagcct tcatcaacca cacccagggc atccccgact
     9241 tctttaagca gtccttccct gagggcttca catgggagag aatcaccaca tacgaagacg
     9301 ggggcgtgct gaccgctacc caggacacca gcttccagaa cggctgcatc atctacaacg
     9361 tcaagatcaa cggggtgaac ttcccatcca acggccctgt gatgcagaag aaaacacgcg
     9421 gctgggaggc caacaccgag atgctgtacc ccgctgacgg cggcctgaga ggccacagcc
     9481 agatggccct gaagctcgtg ggcgggggct acctgcactg ctccttcaag accacataca
     9541 gatccaagaa acccgctaag aacctcaaga tgcccggctt ccacttcgtg gaccacagac
     9601 tggaaagaat caaggaggcc gacaaagaga cctacgtcga gcagcacgag atggctgtgg
     9661 ccaagtactg cgacctccct agcaaactgg ggcacagata acgcgtctgg aacaatcaac
     9721 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     9781 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     9841 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     9901 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     9961 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
    10021 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
    10081 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
    10141 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
    10201 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
    10261 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattctgcag
    10321 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
    10381 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt ttttccagtc acacctcagg
    10441 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
    10501 agaggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
    10561 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
    10621 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
    10681 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
    10741 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
    10801 cagagagtga gaggccttga cattgtttaa actggaatgt gtgtcagtta gggtgtggaa
    10861 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
    10921 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
    10981 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
    11041 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
    11101 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
    11161 tttgcaaaaa gctcccggga gcttgtatat ccattacatg tgagcaaaag gccagcaaaa
    11221 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    11281 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    11341 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    11401 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    11461 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    11521 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    11581 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    11641 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    11701 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    11761 ttgatccggc aaacaaacca ccgctggtag cggttttttt gtttgcaagc agcagattac
    11821 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    11881 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    11941 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    12001 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    12061 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    12121 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    12181 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    12241 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    12301 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    12361 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    12421 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    12481 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    12541 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    12601 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    12661 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    12721 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    12781 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    12841 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    12901 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    12961 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgt
//
