LOCUS       40924_11031        9407 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pCLEAN-S161, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9407)
  AUTHORS   Thole V, Worland B, Snape JW, Vain P.
  TITLE     The pCLEAN dual binary vector system for Agrobacterium-mediated 
            plant transformation
  JOURNAL   Plant Physiol. 145 (4), 1211-1219 (2007)
  PUBMED    17932303
REFERENCE   2  (bases 1 to 9407)
  AUTHORS   Thole V, Vain P.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2007) Department of Crop Genetics, John Innes 
            Centre, Colney Lane, Norwich NR4 7UH, UK
REFERENCE   3  (bases 1 to 9407)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9407)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2007"; volume: "145"; issue: "4"; pages: 
            "1211-1219"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-SEP-2007) Department of Crop Genetics, John Innes Centre, Colney
            Lane, Norwich NR4 7UH, UK"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(7..25)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(43..59)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(67..83)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(91..121)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(136..157)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        404..432
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             480..1667
                     /label=TcR
                     /note="tetracycline efflux protein"
     CDS             2169..3314
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     CDS             4698..5666
                     /label=RepA
                     /note="replication protein of plasmid pSa"
     CDS             6064..6711
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
     oriT            7395..7504
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             7537..7905
                     /label=traJ
                     /note="oriT-recognizing protein"
     rep_origin      8035..8746
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    9246..9270
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    9271..9322
                     /note="inner T-DNA region containing 52 nt-long multiple
                     cloning site with NotI, PacI, SfiI, BsiWI, ApaI, XhoI and 
                     SalI restriction sites"
     misc_feature    9323..9347
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    9348..9371
                     /note="right border overdrive sequence (Peralta et al.
                     (1986) EMBO J 5: 1137-1142)"
ORIGIN
        1 gagtattcta tagtgtcacc taaatagctt ggcgtaatca tggtcatagc tgtttcctgt
       61 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
      121 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
      181 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
      241 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
      301 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      361 atcaggggat aacgcaggaa agaacatgaa ttaattctca tgtttgacag cttatcatcg
      421 attagcttta atgcggtagt ttatcacagt taaattgcta acgcagtcag gcaccgtgta
      481 tgaaatctaa caatgcgctc atcgtcatcc tcggcaccgt caccctggat gctgtaggca
      541 taggcttggt tatgccggta ctgccgggcc tcttgcggga tatcgtccat tccgacagca
      601 tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt gatgcaattt ctatgcgcac
      661 ccgttctcgg agcactgtcc gaccgctttg gccgccgccc agtcctgctc gcttcgctac
      721 ttggagccac tatcgactac gcgatcatgg cgaccacacc cgtcctgtgg attctctacg
      781 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg
      841 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg
      901 gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttacatg
      961 caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa
     1021 tgcaggagtc gcataaggga gagcgccgac cgatgccctt gagagccttc aacccagtca
     1081 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
     1141 tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct
     1201 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc
     1261 tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta
     1321 tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct
     1381 ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc
     1441 aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa ggatcgctcg
     1501 cggctcttac cagcctaact tcgatcactg gaccgctgat cgtcacggcg atttatgccg
     1561 cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta taccttgtct
     1621 gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga atggaagccg
     1681 gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat tcttgcggag
     1741 aactgtgaat gcgcaaacca acccttgtat cggggaagaa cagtatgtcg agctattttt
     1801 tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga
     1861 attgttttag tacctagatg tggcgcaacg atgccggcga caagcaggag cgcaccgact
     1921 tcttccgcat caagtgtttt ggctctcagg ccgaggccca cggcaagtat ttgggcaagg
     1981 ggtcgctggt attcgtgcag ggcaagattc ggaataccaa gtacgagaag gacggccaga
     2041 cggtctacgg gaccgacttc attgccgata aggtggatta tctggacacc aaggcaccag
     2101 gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg agtcggggca atcccgcaag
     2161 gagggtgaat gaatcggacg tttgaccgga aggcatacag gcaagaactg atcgacgcgg
     2221 ggttttccgc cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg
     2281 aaaccttcca gtccgtcggc tcgatggtcc agcaagctac ggccaagatc gagcgcgaca
     2341 gcgtgcaact ggctccccct gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc
     2401 gtctcgaaca ggaggcggca ggtttggcga agtcgatgac catcgacacg cgaggaacta
     2461 tgacgaccaa gaagcgaaaa accgccggcg aggacctggc aaaacaggtc agcgaggcca
     2521 agcaggccgc gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag ctttccttgt
     2581 tcgatattgc gccgtggccg gacacgatgc gagcgatgcc aaacgacacg gcccgctctg
     2641 ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt
     2701 tccacgtcaa caaggacgtg aagatcacct acaccggcgt cgagctgcgg gccgacgatg
     2761 acgaactggt gtggcagcag gtgttggagt acgcgaagcg cacccctatc ggcgagccga
     2821 tcaccttcac gttctacgag ctttgccagg acctgggctg gtcgatcaat ggccggtatt
     2881 acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg
     2941 accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg
     3001 gcaagaaaac gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg
     3061 gcgaccacta cacgaaattc atatgggaga agtaccgcaa gctgtcgccg acggcccgac
     3121 ggatgttcga ctatttcagc tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc
     3181 gcctcatgtg cggatcggat tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag
     3241 cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat gatgacctgg
     3301 tgcattgcaa acgctagggc cttgtggggt cagttccggc tgggggttca gcagccagcg
     3361 ctttactggc atttcaggaa caagcgggca ctgctcgacg cacttgcttc gctcagtatc
     3421 gctcgggacg cacggcgcgc tctacgaact gccgataaac agaggattaa aattgacaat
     3481 tgtgattaag gctcagattc gacggcttgg agcggccgac gtgcaggatt tccgcgagat
     3541 ccgattgtcg gccctgaaga aagctccaga gatgttcggg tccgtttacg agcacgagga
     3601 gaaaaagccc atggaggcgt tcgctgaacg gttgcgagat gccgtggcat tcggcgccta
     3661 catcgacggc gagatcattg ggctgtcggt cttcaaacag gaggacggcc ccaaggacgc
     3721 tcacaaggcg catctgtccg gcgttttcgt ggagcccgaa cagcgaggcc gaggggtcgc
     3781 cggtatgctg ctgcgggcgt tgccggcggg tttattgctc gtgatgatcg tccgacagat
     3841 tccaacggga atctggtgga tgcgcatctt catcctcggc gcacttaata tttcgctatt
     3901 ctggagcttg ttgtttattt cggtctaccg cctgccgggc ggggtcgcgg cgacggtagg
     3961 cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct ctgctaggta gcccgatacg
     4021 attgatggcg gtcctggggg ctatttgcgg aactgcgggc gtggcgctgt tggtgttgac
     4081 accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc ctggcggggg cggtttccat
     4141 ggcgttcgga accgtgctga cccgcaagtg gcaacctccc gtgcctctgc tcacctttac
     4201 cgcctggcaa ctggcggccg gaggacttct gctcgttcca gtagctttag tgtttgatcc
     4261 gccaatcccg atgcctacag gaaccaatgt tctcggcctg gcgtggctcg gcctgatcgg
     4321 agcgggttta acctacttcc tttggttccg ggggatctcg cgactcgaac ctacagttgt
     4381 ttccttactg ggctttctca gccccagatc ctagaggatc gagcggcatt tcattcaggc
     4441 acgtaccacc gaggcgctac aagtcgccag agagcgcggc aagacgctcg ggcgacccaa
     4501 gggcagcaaa tcgagcgcct tgaagctgga cagccgtatt gatgaagtac aggcatacgt
     4561 gaaccttggc ttgccgcaaa gtcgcgcagc cgagttgtta ggcgtcagcc ctcacacctt
     4621 gcgcctgttc atcaaacgcc ggaacatcaa acccacaaac actagaccaa ccatcaccat
     4681 gccggggagg gaacaacatg cctaagaaca acaaagcccc cggccatcgt atcaacgaga
     4741 tcatcaagac gagcctcgcg ctcgaaatgg aggatgcccg cgaagctggc ttagtcggct
     4801 acatggcccg ttgccttgtg caagcgacca tgccccacac cgaccccaag accagctact
     4861 ttgagcgcac caatggcatc gtcaccttgt cgatcatggg caagccgagc atcggcctgc
     4921 cctacggttc tatgccgcgc accttgcttg cttggatatg caccgaggcc gtgcgaacga
     4981 aagaccccgt gttgaacctt ggccggtcgc aatcggaatt tctacaaagg ctcggaatgc
     5041 acaccgatgg ccgttacacg gccacccttc gcaatcaggc gcaacgcctg ttttcatcca
     5101 tgatttcgct tgccggcgag caaggcaatg acttcggcat tgagaacgtc gtcattgcca
     5161 agcgcgcttt tctattctgg aatcccaagc ggccagaaga tcgggcgcta tgggatagca
     5221 ccctcaccct cacaggcgat ttcttcgagg aagtcacccg ctcaccggtt cctatccgaa
     5281 tcgactacct gcacgccttg cggcagtctc cgcttgcgat ggacatttac acgtggctga
     5341 cctatcgcgt gttcctgttg cgggccaagg gccgcccctt cgtgcaaatc ccttgggtcg
     5401 ccctgcaagc gcaattcggc tcatcctatg gcagccgcgc acgcaactcg cccgaactgg
     5461 acgataaggc ccgagagcgg gcagagcggg cagcactcgc cagcttcaaa tacaacttca
     5521 aaaagcgcct acgcgaagtg ttgattgtct atcccgaggc aagcgactgc atcgaagatg
     5581 acggcgaatg cctgcgcatc aaatccacac gcctgcatgt cacccgcgca cccggcaagg
     5641 gcgctcgcat cggcccccct ccgacttgac caggccaacg ctacgcttgg cttggtcaag
     5701 gatcgatctg gcgccggcca gcgagacgag caagattggc cgccgcccga aacgatccga
     5761 cagcgcgccc agcacaggtg cgcaggcaaa ttgcaccaac gcatacagcg ccagcagaat
     5821 gccatagtgg gcggtgacgt cgttcgagtg aaccagatcg cgcaggaggc ccggcagcac
     5881 cggcataatc aggccgatgc cgacagcgtc gagcgcgaca gtgctcagaa ttacgatcag
     5941 gggtatgttg ggtttcacgt ctggcctccg gaccagcctc cgctggtccg attgaacgcg
     6001 cggattcttt atcactgata agttggtgga catattatgt ttatcagtga taaagtgtca
     6061 agcatgacaa agttgcagcc gaatacagtg atccgtgccg ccctggacct gttgaacgag
     6121 gtcggcgtag acggtctgac gacacgcaaa ctggcggaac ggttgggggt tcagcagccg
     6181 gcgctttact ggcacttcag gaacaagcgg gcgctgctcg acgcactggc cgaagccatg
     6241 ctggcggaga atcatacgca ttcggtgccg agagccgacg acgactggcg ctcatttctg
     6301 atcgggaatg cccgcagctt caggcaggcg ctgctcgcct accgcgatgg cgcgcgcatc
     6361 catgccggca cgcgaccggg cgcaccgcag atggaaacgg ccgacgcgca gcttcgcttc
     6421 ctctgcgagg cgggtttttc ggccggggac gccgtcaatg cgctgatgac aatcagctac
     6481 ttcactgttg gggccgtgct tgaggagcag gccggcgaca gcgatgccgg cgagcgcggc
     6541 ggcaccgttg aacaggctcc gctctcgccg ctgttgcggg ccgcgataga cgccttcgac
     6601 gaagccggtc cggacgcagc gttcgagcag ggactcgcgg tgattgtcga tggattggcg
     6661 aaaaggaggc tcgttgtcag gaacgttgaa ggaccgagaa agggtgacga ttgatcagga
     6721 ccgctgccgg agcgcaaccc actcactaca gcagagccat gtagacaaca tcccctcccc
     6781 ctttccaccg cgtcagacgc ccgtagcagc ccgctacggg ctttttcatg ccctgcccta
     6841 gcgtccaagc ctcacggccg cgctcggcct ctctggcggc cttctggcgc tcttccgctt
     6901 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     6961 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7021 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7081 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7141 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7201 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7261 ttttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc
     7321 tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc
     7381 caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc
     7441 ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg
     7501 ccggctaccg ccggcgtaac agatgagggc aagcggatgg ctgatgaaac caagccaacc
     7561 aggaagggca gcccacctat caaggtgtac tgccttccag acgaacgaag agcgattgag
     7621 gaaaaggcgg cggcggccgg catgagcctg tcggcctacc tgctggccgt cggccagggc
     7681 tacaaaatca cgggcgtcgt ggactatgag cacgtccgcg agctggcccg catcaatggc
     7741 gacctgggcc gcctgggcgg cctgctgaaa ctctggctca ccgacgaccc gcgcacggcg
     7801 cggttcggtg atgccacgat cctcgccctg ctggcgaaga tcgaagagaa gcaggacgag
     7861 cttggcaagg tcatgatggg cgtggtccgc ccgagggcag agccatgact tttttagccg
     7921 ctaaaacggc cggggggtgc gcgtgattgc caagcacgtc cccatgcgct ccatcaagaa
     7981 gagcgacttc gcggagctgg tgaagtacat caccgacgag caaggcaaga ccgagcgcct
     8041 ttgcgacgct caccgggctg gttgccctcg ccgctgggct ggcggccgtc tatggccctg
     8101 caaacgcgcc agaaacgccg tcgaagccgt gtgcgagaca ccgcggccgc cggcgttgtg
     8161 gatacctcgc ggaaaacttg gccctcactg acagatgagg ggcggacgtt gacacttgag
     8221 gggccgactc acccggcgcg gcgttgacag atgaggggca ggctcgattt cggccggcga
     8281 cgtggagctg gccagcctcg caaatcggcg aaaacgcctg attttacgcg agtttcccac
     8341 agatgatgtg gacaagcctg gggataagtg ccctgcggta ttgacacttg aggggcgcga
     8401 ctactgacag atgaggggcg cgatccttga cacttgaggg gcagagtgct gacagatgag
     8461 gggcgcacct attgacattt gaggggctgt ccacaggcag aaaatccagc atttgcaagg
     8521 gtttccgccc gtttttcggc caccgctaac ctgtctttta acctgctttt aaaccaatat
     8581 ttataaacct tgtttttaac cagggctgcg ccctgtgcgc gtgaccgcgc acgccgaagg
     8641 ggggtgcccc cccttctcga accctcccgg cccgctaacg cgggcctccc atccccccag
     8701 gggctgcgcc cctcggccgc gaacggcctc accccaaaaa tggcagcgct ggcagtcctt
     8761 gccattgccg ggatcggggc agtaacggga tgggcgatca gcccgagcgc gacgcccgga
     8821 agcattgacg tgccgcaggt gctggcatcg acattcagcg accaggtgcc gggcagtgag
     8881 ggcggcggcc tgggtggcgg cctgcccttc acttcggccg tcggggcatt cacggacttc
     8941 atggcggggc cggcaatttt taccttgggc attcttggca tagtggtcgc gggtgccgtg
     9001 ctcgtgttcg ggggtgcgat aaacccagcg aaccatttga ggtgataggt aagattatac
     9061 cgaggtatga aaacgagaat tggaccttta cagaattact ctatgaagcg ccatatttaa
     9121 aaagctacca agacgaagag gatgaagagg atgaggaggc agattgcctt gaatatattg
     9181 acaatactga taagataata tatcttttat atagaagata attcgagctc ggtacccggg
     9241 gatcttggca ggatatattg tggtgtaaac gttcctgcgg ccgcttaatt aaggccgtac
     9301 gggccctcga gtcgacgttc cttgacagga tatattggcg ggtaaactaa gtcgctgtat
     9361 gtgtttgttt gagatcctct agagtcgacc tgcaggcatg caagctt
//
