LOCUS       40924_11016        7240 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pCLEAN-G184, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7240)
  AUTHORS   Thole V, Worland B, Snape JW, Vain P.
  TITLE     The pCLEAN dual binary vector system for Agrobacterium-mediated 
            plant transformation
  JOURNAL   Plant Physiol. 145 (4), 1211-1219 (2007)
  PUBMED    17932303
REFERENCE   2  (bases 1 to 7240)
  AUTHORS   Thole V, Vain P.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JUL-2011) Department of Crop Genetics, John Innes 
            Centre, Norwich Research Park, Norwich, Norfolk NR4 7UH, United 
            Kingdom
REFERENCE   3  (bases 1 to 7240)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7240)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2007"; volume: "145"; issue: "4"; pages: 
            "1211-1219"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-JUL-2011) Department of Crop Genetics, John Innes Centre, 
            Norwich Research Park, Norwich, Norfolk NR4 7UH, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(61..649)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(823..1635)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1926..2361
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     regulatory      complement(2536..2832)
                     /note="SPA-ter; soybean-derived poly(A) signal"
                     /regulatory_class="terminator"
     CDS             complement(2868..3578)
                     /label=mgfp5
                     /note="GFP with folding enhancement mutations"
     promoter        complement(3677..4021)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    4137..4161
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    4162..4187
                     /note="spacer sequence separating left border No.1 and No.2
                     of the double left border sequence"
     misc_feature    4188..4212
                     /note="nopaline-type T-DNA left border No.2 belonging to a 
                     double left border sequence separated by a spacer sequence 
                     from left border No.1"
     regulatory      complement(4256..4548)
                     /note="SPA-ter; soybean-derived poly(A) signal"
                     /regulatory_class="terminator"
     intron          complement(6050..6238)
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     promoter        complement(6661..7005)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    7158..7182
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    7183..7206
                     /label=right border overdrive sequence
                     /note="right border overdrive sequence"
ORIGIN
        1 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
       61 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      121 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      181 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      241 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      301 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      361 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      421 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      481 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      541 cttcggaaga agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      601 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      661 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      721 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      781 atcaatctaa agtatatatg tgtaacattg gtctagtgat tagaaaaact catcgagcat
      841 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
      901 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
      961 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
     1021 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
     1081 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
     1141 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac
     1201 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
     1261 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
     1321 tgttttccct gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     1381 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
     1441 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
     1501 cccatacaat cggtagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
     1561 cccatataaa tcagcatcca tgttggaatt taatcgcggc cttgagcaag acgtttcccg
     1621 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
     1681 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
     1741 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga
     1801 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa
     1861 caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc
     1921 gatccccatc caacagcccg ccgtcgagcg ggctttttta tccccggaag cctgtggata
     1981 gagggtagtt atccacgtga aaccgctaat gccccgcaaa gccttgattc acggggcttt
     2041 ccggcccgct ccaaaaacta tccacgtgaa atcgctaatc agggtacgtg aaatcgctaa
     2101 tcggagtacg tgaaatcgct aataaggtca cgtgaaatcg ctaatcaaaa aggcacgtga
     2161 gaacgctaat agccctttca gatcaacagc ttgcaaacac ccctcgctcc ggcaagtagt
     2221 tacagcaagt agtatgttca attagctttt caattatgaa tatatatatc aattattggt
     2281 cgcccttggc ttgtggacaa tgcgctacgc gcaccggctc cgcccgtgga caaccgcaag
     2341 cggttgccca ccgtcgagcg cctttgccca caacccggcg gccggccgca acagatcgtt
     2401 ttataaattt ttttttttga aaaagaaaaa gcccgaaagg cggcaacctc tcgggcttct
     2461 ggatttccga tccccggaat tagatccgtt taaacttcga cggtatcgag cttgatatcg
     2521 aattgatccg aattcctttg ttaaccatca tttagtgaca caattaactt aaatttagaa
     2581 gagactaatt tcaacccaca agatggaaat gcaaggacaa tgggttcttg aagttatttg
     2641 gaaacaaagc aacacaacac aatagcttat tcattagttc tcatccatta atttccattg
     2701 aagtccgaaa acagaaacca aaaatacagg atgatgaaat ctcataaagc aaaaacaaat
     2761 gggaatgtag tttaagtaca acaaacaaag tggggtggct acagatgctt tgcaaatttt
     2821 tcaatgactt acggatcaat tcctgcagcc ccttaaagct catcatgttt gtatagttca
     2881 tccatgccat gtgtaatccc agcagctgtt acaaactcaa gaaggaccat gtggtctctc
     2941 ttttcgttgg gatctttcga aagggcagat tgtgtggaca ggtaatggtt gtctggtaaa
     3001 aggacagggc catcgccaat tggagtattt tgttgataat gatcagcgag ttgcacgccg
     3061 ccgtcttcga tgttgtggcg ggtcttgaag ttggctttga tgccgttctt ttgcttgtcg
     3121 gccatgatgt atacgttgtg ggagttgtag ttgtattcca acttgtggcc gaggatgttt
     3181 ccgtcctcct tgaaatcgat tcccttaagc tcgatcctgt tgacgagggt gtctccctca
     3241 aacttgactt cagcacgtgt cttgtagttc ccgtcgtcct tgaagaagat ggtcctctcc
     3301 tgcacgtatc cctcaggcat ggcgctcttg aagaagtcgt gccgcttcat atgatctggg
     3361 tatcttgaaa agcattgaac accataagag aaagtagtga caagtgttgg ccatggaaca
     3421 ggtagttttc cagtagtgca aataaattta agggtaagtt ttccgtatgt tgcatcacct
     3481 tcaccctctc cactgacaga aaatttgtac ccattaacat caccatctaa ttcaacaaga
     3541 attgggacaa ctccagtgaa aagttcttct cctttactga attcggccga ggataatgat
     3601 aggagaagtg aaaagatgag aaagagaaaa agattagtct tcattgttat atctccttgg
     3661 atcctctaga gtcgaggtcc tctccaaatg aaatgaactt ccttatatag aggaagggtc
     3721 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
     3781 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgttcc tcgtgggtgg
     3841 gggtccatct ttgggaccac tgtcggtaga ggcatcttga acgatagcct ttcctttatc
     3901 gcaatgatgg catttgtaga agccatcttc cttttctact gtcctttcga tgaagtgaca
     3961 gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc
     4021 aatagccctc tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc
     4081 gtgctccacc atgttgacct gcaggcatgc aagctggggg atccactaga agatcttggc
     4141 aggatatatt gtggtgtaaa cgttcctgcg gcggtcgaga tggatcttgg caggatatat
     4201 tgtggtgtaa acgttcctgc ggccagcttg atatcgaatt gatccgaatt cctttgttaa
     4261 ccatcattta gtgacacaat taacttaaat ttagaagaga ctaatttcaa cccacaagat
     4321 ggaaatgcaa ggacaatggg ttcttgaagt tatttggaaa caaagcaaca caacacaata
     4381 gcttattcat tagttctcat ccattaattt ccattgaagt ccgaaaacag aaaccaaaaa
     4441 tacaggatga tgaaatctca taaagcaaaa acaaatggga atgtagttta agtacaacaa
     4501 acaaagtggg gtggctacag atgctttgca aatttttcaa tgacttacgg atcaattcct
     4561 gcagccccgg tagcaattcc cgaggctgta gccgacgatg gtgcgccagg agagttgttg
     4621 attcattgtt tgcctccctg ctgcggtttt tcaccgaagt tcatgccagt ccagcgtttt
     4681 tgcagcagaa aagccgccga cttcggtttg cggtcgcgag tgaagatccc tttcttgtta
     4741 ccgccaacgc gcaatatgcc ttgcgaggtc gcaaaatcgg cgaaattcca tacctgttca
     4801 ccgacgacgg cgctgacgcg atcaaagacg cggtgataca tatccagcca tgcacactga
     4861 tactcttcac tccacatgtc ggtgtacatt gagtgcagcc cggctaacgt atccacgccg
     4921 tattcggtga tgataatcgg ctgatgcagt ttctcctgcc aggccagaag ttctttttcc
     4981 agtaccttct ctgccgtttc caaatcgccg ctttggacat accatccgta ataacggttc
     5041 aggcacagca catcaaagag atcgctgatg gtatcggtgt gagcgtcgca gaacattaca
     5101 ttgacgcagg tgatcggacg cgtcgggtcg agtttacgcg ttgcttccgc cagtggcgcg
     5161 aaatattccc gtgcaccttg cggacgggta tccggttcgt tggcaatact ccacatcacc
     5221 acgcttgggt ggtttttgtc acgcgctatc agctctttaa tcgcctgtaa gtgcgcttgc
     5281 tgagtttccc cgttgactgc ctcttcgctg tacagttctt tcggcttgtt gcccgcttcg
     5341 aaaccaatgc ctaaagagag gttaaagccg acagcagcag tttcatcaat caccacgatg
     5401 ccatgttcat ctgcccagtc gagcatctct tcagcgtaag ggtaatgcga ggtacggtag
     5461 gagttggccc caatccagtc cattaatgcg tggtcgtgca ccatcagcac gttatcgaat
     5521 cctttgccac gcaagtccgc atcttcatga cgaccaaagc cagtaaagta gaacggtttg
     5581 tggttaatca ggaactgttc gcccttcact gccactgacc ggatgccgac gcgaagcggg
     5641 tagatatcac actctgtctg gcttttggct gtgacgcaca gttcatagag ataaccttca
     5701 cccggttgcc agaggtgcgg attcaccact tgcaaagtcc cgctagtgcc ttgtccagtt
     5761 gcaaccacct gttgatccgc atcacgcagt tcaacgctga catcaccatt ggccaccacc
     5821 tgccagtcaa cagacgcgtg gttacagtct tgcgcgacat gcgtcaccac ggtgatatcg
     5881 tccacccagg tgttcggcgt ggtgtagagc attacgctgc gatggattcc ggcatagtta
     5941 aagaaatcat ggaagtaaga ctgctttttc ttgccgtttt cgtcggtaat caccattccc
     6001 ggcgggatag tctgccagtt cagttcgttg ttcacacaaa cggtgatacc tgcacatcaa
     6061 caaattttgg tcatatatta gaaaagttat aaattaaaat atacacactt ataaactaca
     6121 gaaaagcaat tgctatatac tacattcttt tattttgaaa aaaatatttg aaatattata
     6181 ttactactaa ttaatgataa ttattatata tatatcaaag gtagaagcag aaacttacgt
     6241 acacttttcc cggcaataac atacggcgtg acatcggctt caaatggcgt atagccgccc
     6301 tgatgctcca tcacttcctg attattgacc cacactttgc cgtaatgagt gaccgcatcg
     6361 aaacgcagca cgatacgctg gcctgcccaa cctttcggta taaagacttc gcgctgatac
     6421 cagacgttgc ccgcataatt acgaatatct gcatcggcga actgatcgtt aaaactgcct
     6481 ggcacagcaa ttgcccggct ttcttgtaac gcgctttccc accaacgctg accaattcca
     6541 cagttttcgc gatccagact gaatgcccac aggccgtcga gttttttgat ttcacgggtt
     6601 ggggtttcta caggacgtaa cataagggac tgacctaccc ggggatcctc tagagtcgag
     6661 gtcctctcca aatgaaatga acttccttat atagaggaag ggtcttgcga aggatagtgg
     6721 gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac
     6781 gtggttggaa cgtcttcttt ttccacgatg ttcctcgtgg gtgggggtcc atctttggga
     6841 ccactgtcgg tagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg
     6901 tagaagccat cttccttttc tactgtcctt tcgatgaagt gacagatagc tgggcaatgg
     6961 aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctctggtct
     7021 tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgttg
     7081 acctgcaggc atgcaagctg ggggatccac tagggccgct taattaaggc cgtacgggcc
     7141 ctcgagtcga cgttccttga caggatatat tggcgggtaa actaagtcgc tgtatgtgtt
     7201 tgtttgagat cctctagggc atgcaagctg atctggatct
//
