LOCUS       40924_11006        7171 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pCLEAN-G156, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7171)
  AUTHORS   Thole V, Worland B, Snape JW, Vain P.
  TITLE     The pCLEAN dual binary vector system for Agrobacterium-mediated 
            plant transformation
  JOURNAL   Plant Physiol. 145 (4), 1211-1219 (2007)
  PUBMED    17932303
REFERENCE   2  (bases 1 to 7171)
  AUTHORS   Thole V, Vain P.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2011) Department of Crop Genetics, John Innes 
            Centre, Norwich Research Park, Norwich, Norfolk NR4 7UH, United 
            Kingdom
REFERENCE   3  (bases 1 to 7171)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7171)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2007"; volume: "145"; issue: "4"; pages: 
            "1211-1219"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-SEP-2011) Department of Crop Genetics, John Innes Centre, 
            Norwich Research Park, Norwich, Norfolk NR4 7UH, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(61..649)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(823..1635)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1926..2361
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     regulatory      complement(2538..2834)
                     /note="SPA-ter; soybean-derived poly(A) signal"
                     /regulatory_class="terminator"
     CDS             complement(2870..3580)
                     /label=mgfp5
                     /note="GFP with folding enhancement mutations"
     promoter        complement(3679..4023)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    4141..4165
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      complement(4209..4501)
                     /note="SPA-ter; soybean-derived poly(A) signal"
                     /regulatory_class="terminator"
     intron          complement(6003..6191)
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     promoter        complement(6614..6958)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    7111..7135
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    7136..7159
                     /label=right border overdrive sequence
                     /note="right border overdrive sequence"
ORIGIN
        1 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
       61 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      121 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      181 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      241 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      301 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      361 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      421 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      481 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      541 cttcggaaga agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      601 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      661 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      721 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      781 atcaatctaa agtatatatg tgtaacattg gtctagtgat tagaaaaact catcgagcat
      841 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
      901 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
      961 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
     1021 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
     1081 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
     1141 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac
     1201 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
     1261 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
     1321 tgttttccct gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     1381 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
     1441 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
     1501 cccatacaat cggtagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
     1561 cccatataaa tcagcatcca tgttggaatt taatcgcggc cttgagcaag acgtttcccg
     1621 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
     1681 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
     1741 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga
     1801 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa
     1861 caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc
     1921 gatccccatc caacagcccg ccgtcgagcg ggctttttta tccccggaag cctgtggata
     1981 gagggtagtt atccacgtga aaccgctaat gccccgcaaa gccttgattc acggggcttt
     2041 ccggcccgct ccaaaaacta tccacgtgaa atcgctaatc agggtacgtg aaatcgctaa
     2101 tcggagtacg tgaaatcgct aataaggtca cgtgaaatcg ctaatcaaaa aggcacgtga
     2161 gaacgctaat agccctttca gatcaacagc ttgcaaacac ccctcgctcc ggcaagtagt
     2221 tacagcaagt agtatgttca attagctttt caattatgaa tatatatatc aattattggt
     2281 cgcccttggc ttgtggacaa tgcgctacgc gcaccggctc cgcccgtgga caaccgcaag
     2341 cggttgccca ccgtcgagcg cctttgccca caacccggcg gccggccgca acagatcgtt
     2401 ttataaattt ttttttttga aaaagaaaaa gcccgaaagg cggcaacctc tcgggcttct
     2461 ggatttccga tccccggaat tagatccgtt taaactactc gacggtatcg agcttgatat
     2521 cgaattgatc cgaattcctt tgttaaccat catttagtga cacaattaac ttaaatttag
     2581 aagagactaa tttcaaccca caagatggaa atgcaaggac aatgggttct tgaagttatt
     2641 tggaaacaaa gcaacacaac acaatagctt attcattagt tctcatccat taatttccat
     2701 tgaagtccga aaacagaaac caaaaataca ggatgatgaa atctcataaa gcaaaaacaa
     2761 atgggaatgt agtttaagta caacaaacaa agtggggtgg ctacagatgc tttgcaaatt
     2821 tttcaatgac ttacggatca attcctgcag ccccttaaag ctcatcatgt ttgtatagtt
     2881 catccatgcc atgtgtaatc ccagcagctg ttacaaactc aagaaggacc atgtggtctc
     2941 tcttttcgtt gggatctttc gaaagggcag attgtgtgga caggtaatgg ttgtctggta
     3001 aaaggacagg gccatcgcca attggagtat tttgttgata atgatcagcg agttgcacgc
     3061 cgccgtcttc gatgttgtgg cgggtcttga agttggcttt gatgccgttc ttttgcttgt
     3121 cggccatgat gtatacgttg tgggagttgt agttgtattc caacttgtgg ccgaggatgt
     3181 ttccgtcctc cttgaaatcg attcccttaa gctcgatcct gttgacgagg gtgtctccct
     3241 caaacttgac ttcagcacgt gtcttgtagt tcccgtcgtc cttgaagaag atggtcctct
     3301 cctgcacgta tccctcaggc atggcgctct tgaagaagtc gtgccgcttc atatgatctg
     3361 ggtatcttga aaagcattga acaccataag agaaagtagt gacaagtgtt ggccatggaa
     3421 caggtagttt tccagtagtg caaataaatt taagggtaag ttttccgtat gttgcatcac
     3481 cttcaccctc tccactgaca gaaaatttgt acccattaac atcaccatct aattcaacaa
     3541 gaattgggac aactccagtg aaaagttctt ctcctttact gaattcggcc gaggataatg
     3601 ataggagaag tgaaaagatg agaaagagaa aaagattagt cttcattgtt atatctcctt
     3661 ggatcctcta gagtcgaggt cctctccaaa tgaaatgaac ttccttatat agaggaaggg
     3721 tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa
     3781 tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgtt cctcgtgggt
     3841 gggggtccat ctttgggacc actgtcggta gaggcatctt gaacgatagc ctttccttta
     3901 tcgcaatgat ggcatttgta gaagccatct tccttttcta ctgtcctttc gatgaagtga
     3961 cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc
     4021 tcaatagccc tctggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg
     4081 tcgtgctcca ccatgttgac ctgcaggcat gcaagctggg ggatccacta ggtaagatct
     4141 tggcaggata tattgtggtg taaacgttcc tgcggccagc ttgatatcga attgatccga
     4201 attcctttgt taaccatcat ttagtgacac aattaactta aatttagaag agactaattt
     4261 caacccacaa gatggaaatg caaggacaat gggttcttga agttatttgg aaacaaagca
     4321 acacaacaca atagcttatt cattagttct catccattaa tttccattga agtccgaaaa
     4381 cagaaaccaa aaatacagga tgatgaaatc tcataaagca aaaacaaatg ggaatgtagt
     4441 ttaagtacaa caaacaaagt ggggtggcta cagatgcttt gcaaattttt caatgactta
     4501 cggatcaatt cctgcagccc cggtagcaat tcccgaggct gtagccgacg atggtgcgcc
     4561 aggagagttg ttgattcatt gtttgcctcc ctgctgcggt ttttcaccga agttcatgcc
     4621 agtccagcgt ttttgcagca gaaaagccgc cgacttcggt ttgcggtcgc gagtgaagat
     4681 ccctttcttg ttaccgccaa cgcgcaatat gccttgcgag gtcgcaaaat cggcgaaatt
     4741 ccatacctgt tcaccgacga cggcgctgac gcgatcaaag acgcggtgat acatatccag
     4801 ccatgcacac tgatactctt cactccacat gtcggtgtac attgagtgca gcccggctaa
     4861 cgtatccacg ccgtattcgg tgatgataat cggctgatgc agtttctcct gccaggccag
     4921 aagttctttt tccagtacct tctctgccgt ttccaaatcg ccgctttgga cataccatcc
     4981 gtaataacgg ttcaggcaca gcacatcaaa gagatcgctg atggtatcgg tgtgagcgtc
     5041 gcagaacatt acattgacgc aggtgatcgg acgcgtcggg tcgagtttac gcgttgcttc
     5101 cgccagtggc gcgaaatatt cccgtgcacc ttgcggacgg gtatccggtt cgttggcaat
     5161 actccacatc accacgcttg ggtggttttt gtcacgcgct atcagctctt taatcgcctg
     5221 taagtgcgct tgctgagttt ccccgttgac tgcctcttcg ctgtacagtt ctttcggctt
     5281 gttgcccgct tcgaaaccaa tgcctaaaga gaggttaaag ccgacagcag cagtttcatc
     5341 aatcaccacg atgccatgtt catctgccca gtcgagcatc tcttcagcgt aagggtaatg
     5401 cgaggtacgg taggagttgg ccccaatcca gtccattaat gcgtggtcgt gcaccatcag
     5461 cacgttatcg aatcctttgc cacgcaagtc cgcatcttca tgacgaccaa agccagtaaa
     5521 gtagaacggt ttgtggttaa tcaggaactg ttcgcccttc actgccactg accggatgcc
     5581 gacgcgaagc gggtagatat cacactctgt ctggcttttg gctgtgacgc acagttcata
     5641 gagataacct tcacccggtt gccagaggtg cggattcacc acttgcaaag tcccgctagt
     5701 gccttgtcca gttgcaacca cctgttgatc cgcatcacgc agttcaacgc tgacatcacc
     5761 attggccacc acctgccagt caacagacgc gtggttacag tcttgcgcga catgcgtcac
     5821 cacggtgata tcgtccaccc aggtgttcgg cgtggtgtag agcattacgc tgcgatggat
     5881 tccggcatag ttaaagaaat catggaagta agactgcttt ttcttgccgt tttcgtcggt
     5941 aatcaccatt cccggcggga tagtctgcca gttcagttcg ttgttcacac aaacggtgat
     6001 acctgcacat caacaaattt tggtcatata ttagaaaagt tataaattaa aatatacaca
     6061 cttataaact acagaaaagc aattgctata tactacattc ttttattttg aaaaaaatat
     6121 ttgaaatatt atattactac taattaatga taattattat atatatatca aaggtagaag
     6181 cagaaactta cgtacacttt tcccggcaat aacatacggc gtgacatcgg cttcaaatgg
     6241 cgtatagccg ccctgatgct ccatcacttc ctgattattg acccacactt tgccgtaatg
     6301 agtgaccgca tcgaaacgca gcacgatacg ctggcctgcc caacctttcg gtataaagac
     6361 ttcgcgctga taccagacgt tgcccgcata attacgaata tctgcatcgg cgaactgatc
     6421 gttaaaactg cctggcacag caattgcccg gctttcttgt aacgcgcttt cccaccaacg
     6481 ctgaccaatt ccacagtttt cgcgatccag actgaatgcc cacaggccgt cgagtttttt
     6541 gatttcacgg gttggggttt ctacaggacg taacataagg gactgaccta cccggggatc
     6601 ctctagagtc gaggtcctct ccaaatgaaa tgaacttcct tatatagagg aagggtcttg
     6661 cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac atcaatccac
     6721 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgttcctcg tgggtggggg
     6781 tccatctttg ggaccactgt cggtagaggc atcttgaacg atagcctttc ctttatcgca
     6841 atgatggcat ttgtagaagc catcttcctt ttctactgtc ctttcgatga agtgacagat
     6901 agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat
     6961 agccctctgg tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg
     7021 ctccaccatg ttgacctgca ggcatgcaag ctgggggatc cactagggcc gcttaattaa
     7081 ggccgtacgg gccctcgagt cgacgttcct tgacaggata tattggcggg taaactaagt
     7141 cgctgtatgt gtttgtttga gatctggatc t
//
