LOCUS       62056_6500        5183 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    732..753
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        768..798
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    806..822
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     830..846
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(859..915)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(916..932)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1458..2246)
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(3501..4448)
                     /codon_start=1
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      complement(4496..4718)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
ORIGIN
        1 aattctgcta gacctttgct ggaaaacttg taaattctgc tagaccctct gtaaattccg
       61 ctagaccttt gtgtgttttt tttgtttata ttcaagtggt tataatttat agaataaaga
      121 aagaataaaa aaagataaaa agaatagatc ccagccctgt gtataactca ctactttagt
      181 cagttccgca gtattacaaa aggatgtcgc aaacgctgtt tgctcctcta caaaacagac
      241 cttaaaaccc taaaggctta agtagcaccc tcgcaagctc gggcaaatcg ctgaatattc
      301 cttttgtctc cgaccatcag gcacctgagt cgctgtcttt ttcgtgacat tcagttcgct
      361 gcgctcacgg ctctggcagt gaatgggggt aaatggcact acaggcgcct tttatggatt
      421 catgcaagga aactacccat aatacaagaa aagcccgtca cgggcttctc agggcgtttt
      481 atggcgggtc tgctatgtgg tgctatctga ctttttgctg ttcagcagtt cctgccctct
      541 gattttccag tctgaccact tcggattatc ccgtgacagg tcattcagac tggctaatgc
      601 acccagtaag gcagcggtat catcaacagg cttacccgtc ttactgtcgg gaattcgcgt
      661 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
      721 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
      781 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
      841 tatgaccatg attacgccaa gcttgcatgc ctgcaggtcg actctagagg atccccgggt
      901 accgagctcg aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
      961 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     1021 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
     1081 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     1141 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     1201 cgagcttagt aaagccctcg ctagatttta atgcggatgt tgcgattact tcgccaacta
     1261 ttgcgataac aagaaaaagc cagcctttca tgatatatct cccaatttgt gtagggctta
     1321 ttatgcacgc ttaaaaataa taaaagcaga cttgacctga tagtttggct gtgagcaatt
     1381 atgtgcttag tgcatctaac gcttgagtta agccgcgccg cgaagcggcg tcggcttgaa
     1441 cgaattgtta gacattattt gccgactacc ttggtgatct cgcctttcac gtagtggaca
     1501 aattcttcca actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc
     1561 tgtctagctt caagtatgac gggctgatac tgggccggca ggcgctccat tgcccagtcg
     1621 gcagcgacat ccttcggcgc gattttgccg gttactgcgc tgtaccaaat gcgggacaac
     1681 gtaagcacta catttcgctc atcgccagcc cagtcgggcg gcgagttcca tagcgttaag
     1741 gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc
     1801 gctggaccta ccaaggcaac gctatgttct cttgcttttg tcagcaagat agccagatca
     1861 atgtcgatcg tggctggctc gaagatacct gcaagaatgt cattgcgctg ccattctcca
     1921 aattgcagtt cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg cacaacaatg
     1981 gtgacttcta cagcgcggag aatctcgctc tctccagggg aagccgaagt ttccaaaagg
     2041 tcgttgatca aagctcgccg cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa
     2101 tcaatatcac tgtgtggctt caggccgcca tccactgcgg agccgtacaa atgtacggcc
     2161 agcaacgtcg gttcgagatg gcgctcgatg acgccaacta cctctgatag ttgagtcgat
     2221 acttcggcga tcaccgcttc cctcatgatg tttaactttg ttttagggcg actgccctgc
     2281 tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc
     2341 tgcttggatg cccgaggcat agactgtacc ccaaaaaaac agtcataaca agccatgaaa
     2401 accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag
     2461 cgcatacgct acttgcatta cagcttacga accgaacagg cttatgtcca ctgggttcgt
     2521 gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct tgggcagcag cgaagtcgag
     2581 gcatttctgt cctggctggc gaacgagcgc aaggtttcgg tctccacgca tcgtcaggca
     2641 ttggcggcct tgctgttctt ctacggcaag gtgctgtgca cggatctgcc ctggcttcag
     2701 gagatcggaa gacctcggcc gtcgcggcgc ttgccggtgg tgctgacccc ggatgaagtg
     2761 gttcgcatcc tcggttttct ggaaggcgag catcgtttgt tcgcccagct tctgtatgga
     2821 acgggcatgc ggatcagtga gggtttgcaa ctgcgggtca aggatctgga tttcgatcac
     2881 ggcacgatca tcgtgcggga gggcaagggc tccaaggatc gggccttgat gttacccgag
     2941 agcttggcac ccagcctgcg cgagcagggg aattaattcc cacgggtttt gctgcccgca
     3001 aacgggctgt tctggtgttg ctagtttgtt atcagaatcg cagatccggc ttcagccggt
     3061 ttgccggctg aaagcgctat ttcttccaga attgccatga ttttttcccc acgggaggcg
     3121 tcactggctc ccgtgttgtc ggcagctttg attcgataag cagcatcgcc tgtttcaggc
     3181 tgtctatgtg tgactgttga gctgtaacaa gttgtctcag gtgttcaatt tcatgttcta
     3241 gttgctttgt tttactggtt tcacctgttc tattaggtgt tacatgctgt tcatctgtta
     3301 cattgtcgat ctgttcatgg tgaacagctt tgaatgcacc aaaaactcgt aaaagctctg
     3361 atgtatctat cttttttaca ccgttttcat ctgtgcatat ggacagtttt ccctttgata
     3421 tgtaacggtg aacagttgtt ctacttttgt ttgttagtct tgatgcttca ctgatagata
     3481 caagagccat aagaacctca gatccttccg tatttagcca gtatgttctc tagtgtggtt
     3541 cgttgttttt gcgtgagcca tgagaacgaa ccattgagat catacttact ttgcatgtca
     3601 ctcaaaaatt ttgcctcaaa actggtgagc tgaatttttg cagttaaagc atcgtgtagt
     3661 gtttttctta gtccgttatg taggtaggaa tctgatgtaa tggttgttgg tattttgtca
     3721 ccattcattt ttatctggtt gttctcaagt tcggttacga gatccatttg tctatctagt
     3781 tcaacttgga aaatcaacgt atcagtcggg cggcctcgct tatcaaccac caatttcata
     3841 ttgctgtaag tgtttaaatc tttacttatt ggtttcaaaa cccattggtt aagcctttta
     3901 aactcatggt agttattttc aagcattaac atgaacttaa attcatcaag gctaatctct
     3961 atatttgcct tgtgagtttt cttttgtgtt agttctttta ataaccactc ataaatcctc
     4021 atagagtatt tgttttcaaa agacttaaca tgttccagat tatattttat gaattttttt
     4081 aactggaaaa gataaggcaa tatctcttca ctaaaaacta attctaattt ttcgcttgag
     4141 aacttggcat agtttgtcca ctggaaaatc tcaaagcctt taaccaaagg attcctgatt
     4201 tccacagttc tcgtcatcag ctctctggtt gctttagcta atacaccata agcattttcc
     4261 ctactgatgt tcatcatctg agcgtattgg ttataagtga acgataccgt ccgttctttc
     4321 cttgtagggt tttcaatcgt ggggttgagt agtgccacac agcataaaat tagcttggtt
     4381 tcatgctccg ttaagtcata gcgactaatc gctagttcat ttgctttgaa aacaactaat
     4441 tcagacatac atctcaattg gtctaggtga ttttaatcac tataccaatt gagatgggct
     4501 agtcaatgat aattactagt ccttttcctt tgagttgtgg gtatctgtaa attctgctag
     4561 acctttgctg gaaaacttgt aaattctgct agaccctctg taaattccgc tagacctttg
     4621 tgtgtttttt ttgtttatat tcaagtggtt ataatttata gaataaagaa agaataaaaa
     4681 aagataaaaa gaatagatcc cagccctgtg tataactcac tactttagtc agttccgcag
     4741 tattacaaaa ggatgtcgca aacgctgttt gctcctctac aaaacagacc ttaaaaccct
     4801 aaaggcttaa gtagcaccct cgcaagctcg ggcaaatcgc tgaatattcc ttttgtctcc
     4861 gaccatcagg cacctgagtc gctgtctttt tcgtgacatt cagttcgctg cgctcacggc
     4921 tctggcagtg aatgggggta aatggcacta caggcgcctt ttatggattc atgcaaggaa
     4981 actacccata atacaagaaa agcccgtcac gggcttctca gggcgtttta tggcgggtct
     5041 gctatgtggt gctatctgac tttttgctgt tcagcagttc ctgccctctg attttccagt
     5101 ctgaccactt cggattatcc cgtgacaggt cattcagact ggctaatgca cccagtaagg
     5161 cagcggtatc atcaacaggc tta
//
