LOCUS       V008470                 7487 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008470
VERSION     V008470
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7487)
  AUTHORS   Bayne AC, Boltz D, Owen C, Betz Y, Maia G, Azadi P, Archer-Hartmann
            S, Zirkle R, Lippmeier JC.
  TITLE     Vaccination against Influenza with Recombinant Hemagglutinin
            Expressed by Schizochytrium sp. Confers Protective Immunity
  JOURNAL   PLoS ONE 8 (4), E61790 (2013)
   PUBMED   23626728
REFERENCE   2  (bases 1 to 7487)
  AUTHORS   Bayne A-C.V., Lippmeier JC.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-NOV-2012) Molecular Biology, DSM Nutritional Products,
            6480 Dobbin Rd., Columbia, MD 21045, USA
REFERENCE   3  (bases 1 to 7487)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7487)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2013"; volume: "8"; issue: "4"; pages: "E61790"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-NOV-2012) Molecular Biology, DSM Nutritional Products, 6480
            Dobbin Rd., Columbia, MD 21045, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..1063
                     /label="elongation factor 1 promoter"
                     /note="elongation factor 1 promoter"
                     /regulatory_class="promoter"
     CDS             1070..2773
                     /gene="HA"
                     /label="Hemagglutinin"
                     /note="Hemagglutinin from Influenza A virus (strain
                     A/Chicken/Hong Kong/220/1997 H5N1 genotype Gs/Gd).
                     Accession#: O89746"
     regulatory      2796..3435
                     /label="Pfa 3 terminator"
                     /note="Pfa 3 terminator"
                     /regulatory_class="terminator"
     polyA_signal    3578..3699
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3961..4549)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4723..5580)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5581..5685)
                     /label="AmpR promoter"
     regulatory      5970..6418
                     /label="alpha-tubulin promoter"
                     /note="alpha-tubulin promoter"
                     /regulatory_class="promoter"
     CDS             6422..7210
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"
     polyA_signal    7343..7476
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ctcttatctg cctcgcgccg ttgaccgccg cttgactctt ggcgcttgcc gctcgcatcc
       61 tgcctcgctc gcgcaggcgg gcgggcgagt gggtgggtcc gcagccttcc gcgctcgccc
      121 gctagctcgc tcgcgccgtg ctgcagccag cagggcagca ccgcacggca ggcaggtccc
      181 ggcgcggatc gatcgatcca tcgatccatc gatccatcga tcgtgcggtc aaaaagaaag
      241 gaagaagaaa ggaaaaagaa aggcgtgcgc acccgagtgc gcgctgagcg cccgctcgcg
      301 gtcccgcgga gcctccgcgt tagtccccgc cccgcgccgc gcagtccccc gggaggcatc
      361 gcgcacctct cgccgccccc tcgcgcctcg ccgattcccc gcctcccctt ttccgcttct
      421 tcgccgcctc cgctcgcggc cgcgtcgccc gcgccccgct ccctatctgc tccccagggg
      481 ggcactccgc accttttgcg cccgctgccg ccgccgcggc cgccccgccg ccctggtttc
      541 ccccgcgagc gcggccgcgt cgccgcgcaa agactcgccg cgtgccgccc cgagcaacgg
      601 gtggcggcgg cgcggcggcg ggcggggcgc ggcggcgcgt aggcggggct aggcgccggc
      661 taggcgaaac gccgcccccg ggcgccgccg ccgcccgctc cagagcagtc gccgcgccag
      721 accgccaacg cagagaccga gaccgaggta cgtcgcgccc gagcacgccg cgacgcgcgg
      781 cagggacgag gagcacgacg ccgcgccgcg ccgcgcgggg ggggggaggg agaggcagga
      841 cgcgggagcg agcgtgcatg tttccgcgcg agacgacgcc gcgcgcgctg gagaggagat
      901 aaggcgcttg gatcgcgaga gggccagcca ggctggaggc gaaaatgggt ggagaggata
      961 gtatcttgcg tgcttggacg aggagactga cgaggaggac ggatacgtcg atgatgatgt
     1021 gcacagagaa gaagcagttc gaaagcgact actagcaagc aagggatcca tggagaagat
     1081 tgtcctcctc cttgccactg tctcccttgt caagagcgat cagatttgca tcggctacca
     1141 cgctaacaac tccaccgagc aggttgacac catcatggag aagaacgtta ccgtcactca
     1201 cgcccaggac attctcgagc gcacccacaa cggcaagctc tgcgacctga acggagtcaa
     1261 gcccctgatc ctccgcgatt gctccgtcgc cggctggctt ctcggcaacc ctatgtgcga
     1321 cgagtttatt aacgtccctg agtggtccta catcgtcgag aaggcctccc ccgctaacga
     1381 cctctgctac cctggtaact tcaacgacta cgaggagctc aagcacctcc tctcccgcat
     1441 ttcccacttt gagaagatcc agattatccc caagtccagc tggtccaacc atgacgcctc
     1501 cagcggtgtc tcctccgcct gcccgtacct cggcaagtcg agcttttttc gcaacgtcgt
     1561 ctggctcatc aagaagaact ccacttaccc caccatcaag cgcagctaca acaacacgaa
     1621 ccaggaggac ctcctcgtcc tttggggcat tcaccaccct aacgacgccg ccgagcagac
     1681 caagctctac cagaacccca ccacttacat cagcgtcggc acctcgactc tcaaccagcg
     1741 ccttgtcccc gagatcgcca cccgccctaa ggttaacgga cagtccggcc gtattgagtt
     1801 tttttggacc attctcaagc ccaacgacgc catcaacttc gagtccaacg gaaactttat
     1861 tgcccctgag tacgcttaca agatcgtcaa gaagggtgac tccaccatca tgaagagcga
     1921 gcttgagtac ggcaactgca acaccaagtg ccagaccccg atgggcgcca ttaactcctc
     1981 catgcctttc cacaacattc atcccctcac catcggcgag tgccccaagt acgtcaagtc
     2041 caaccgtctc gtcctcgcca ctggcctccg caacgccccc cagcgcgagc gtcgccgcaa
     2101 gaagcgcggt ctctttggcg ccattgccgg ttttatcgag ggtggttggc agggaatggt
     2161 cgacggatgg tacggatacc accactcgaa cgagcagggc tccggctacg ccgctgacca
     2221 ggagtccacc cagaaggcta tcgacggtgt caccaacaag gttaactcca ttattaacaa
     2281 gatgaacact cagtttgagg ccgttggccg tgagtttaac aacctcgagc gtcgtattga
     2341 gaacctcaac aagaagatgg aggacggctt cctcgacgtt tggacgtaca acgctgagct
     2401 cctcgtcctc atggagaacg agcgtacgct cgactttcac gactcgaacg ttaagaacct
     2461 ctacgacaag gtgcgcctgc agctccgcga taacgccaag gagcttggaa acggctgctt
     2521 tgagttctac cacaagtgcg ataacgagtg catggagtcg gtcaagaacg gaacctacga
     2581 ttaccctcag tactccgagg aggcccgtct caaccgcgag gagatttccg gcgtcaagct
     2641 cgagtccatg ggcacctacc agatccttag cctctactcc accgtcgctt cctccctcgc
     2701 cctcgccatt atggtcgccg gcctctcgct ctggatgtgc tccaacggct ccctccagtg
     2761 ccgcatctgc atttaacata tgagttatga gatccgaaag tgaaccttgt cctaacccga
     2821 cagcgaatgg cgggaggggg cgggctaaaa gatcgtatta catagtattt ttcccctact
     2881 ctttgtgttt gtcttttttt tttttttgaa cgcattcaag ccacttgtct gggtttactt
     2941 gtttgtttgc ttgcttgctt gcttgcttgc ctgcttcttg gtcagacggc ccaaaaaagg
     3001 gaaaaaattc attcatggca cagataagaa aaagaaaaag tttgtcgacc accgtcatca
     3061 gaaagcaaga gaagagaaac actcgcgctc acattctcgc tcgcgtaaga atcttagcca
     3121 cgcatacgaa gtaatttgtc catctggcga atctttacat gagcgttttc aagctggagc
     3181 gtgagatcat acctttcttg atcgtaatgt tccaaccttg cataggcctc gttgcgatcc
     3241 gctagcaatg cgtcgtactc ccgttgcaac tgcgccatcg cctcattgtg acgtgagttc
     3301 agattcttct cgagaccttc gagcgctgct aatttcgcct gacgctcctt cttttgtgct
     3361 tccatgacac gccgcttcac cgtgcgttcc acttcttcct cagacatgcc cttggctgcc
     3421 tcgacctgct cggtaaaacg ggccccagca cgtgctacga gatttcgatt ccaccgccgc
     3481 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     3541 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     3601 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     3661 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catacatggt cgacctgcag
     3721 gaacctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     3781 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3841 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3901 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3961 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4021 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4081 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4141 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4201 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4261 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4321 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4381 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     4441 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4501 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4561 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4621 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     4681 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     4741 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     4801 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     4861 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     4921 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     4981 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     5041 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     5101 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     5161 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     5221 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     5281 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     5341 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     5401 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     5461 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     5521 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     5581 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     5641 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     5701 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     5761 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     5821 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     5881 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     5941 gcagattgta ctgagagtgc accaagcttc caattttagg ccccccactg accgaggtct
     6001 gtcgataatc cacttttcca ttgattttcc aggtttcgtt aactcatgcc actgagcaaa
     6061 acttcggtct ttcctaacaa aagctctcct cacaaagcat ggcgcggcaa cggacgtgtc
     6121 ctcatactcc actgccacac aaggtcgata aactaagctc ctcacaaata gaggagaatt
     6181 ccactgacaa ctgaaaacaa tgtatgagag acgatcacca ctggagcggc gcggcggttg
     6241 ggcgcggagg tcggcagcaa aaacaagcga ctcgccgagc aaacccgaat cagccttcag
     6301 acggtcgtgc ctaacaacac gccgttctac cccgccttct tcgcgcccct tcgcgtccaa
     6361 gcatccttca agtttatctc tctagttcaa cttcaagaag aacaacacca ccaacaccat
     6421 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     6481 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     6541 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     6601 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     6661 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     6721 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     6781 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     6841 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     6901 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     6961 cgatgatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     7021 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     7081 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     7141 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     7201 cgagttcttc tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
     7261 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     7321 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     7381 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7441 gtccaaactc atcaatgtat cttatcatgt ctgaattccc ggggtac
//
