LOCUS       V008471                 7478 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008471
VERSION     V008471
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7478)
  AUTHORS   Bayne AC, Boltz D, Owen C, Betz Y, Maia G, Azadi P, Archer-Hartmann
            S, Zirkle R, Lippmeier JC.
  TITLE     Vaccination against Influenza with Recombinant Hemagglutinin
            Expressed by Schizochytrium sp. Confers Protective Immunity
  JOURNAL   PLoS ONE 8 (4), E61790 (2013)
   PUBMED   23626728
REFERENCE   2  (bases 1 to 7478)
  AUTHORS   Bayne A-C.V., Lippmeier JC.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-NOV-2012) Molecular Biology, DSM Nutritional Products,
            6480 Dobbin Rd., Columbia, MD 21045, USA
REFERENCE   3  (bases 1 to 7478)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7478)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2013"; volume: "8"; issue: "4"; pages: "E61790"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-NOV-2012) Molecular Biology, DSM Nutritional Products, 6480
            Dobbin Rd., Columbia, MD 21045, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7478
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..1063
                     /label="elongation factor 1 promoter"
                     /note="elongation factor 1 promoter"
                     /regulatory_class="promoter"
     CDS             1070..2764
                     /gene="HA"
                     /label="Hemagglutinin"
                     /note="Hemagglutinin from Influenza A virus (strain
                     A/Puerto Rico/8/1934 H1N1). Accession#: P03452"
     regulatory      2787..3426
                     /label="Pfa 3 terminator"
                     /note="Pfa 3 terminator"
                     /regulatory_class="terminator"
     polyA_signal    3569..3690
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3952..4540)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4714..5571)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5572..5676)
                     /label="AmpR promoter"
     regulatory      5961..6409
                     /label="alpha-tubulin promoter"
                     /note="alpha-tubulin promoter"
                     /regulatory_class="promoter"
     CDS             6413..7201
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"
     polyA_signal    7334..7467
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ctcttatctg cctcgcgccg ttgaccgccg cttgactctt ggcgcttgcc gctcgcatcc
       61 tgcctcgctc gcgcaggcgg gcgggcgagt gggtgggtcc gcagccttcc gcgctcgccc
      121 gctagctcgc tcgcgccgtg ctgcagccag cagggcagca ccgcacggca ggcaggtccc
      181 ggcgcggatc gatcgatcca tcgatccatc gatccatcga tcgtgcggtc aaaaagaaag
      241 gaagaagaaa ggaaaaagaa aggcgtgcgc acccgagtgc gcgctgagcg cccgctcgcg
      301 gtcccgcgga gcctccgcgt tagtccccgc cccgcgccgc gcagtccccc gggaggcatc
      361 gcgcacctct cgccgccccc tcgcgcctcg ccgattcccc gcctcccctt ttccgcttct
      421 tcgccgcctc cgctcgcggc cgcgtcgccc gcgccccgct ccctatctgc tccccagggg
      481 ggcactccgc accttttgcg cccgctgccg ccgccgcggc cgccccgccg ccctggtttc
      541 ccccgcgagc gcggccgcgt cgccgcgcaa agactcgccg cgtgccgccc cgagcaacgg
      601 gtggcggcgg cgcggcggcg ggcggggcgc ggcggcgcgt aggcggggct aggcgccggc
      661 taggcgaaac gccgcccccg ggcgccgccg ccgcccgctc cagagcagtc gccgcgccag
      721 accgccaacg cagagaccga gaccgaggta cgtcgcgccc gagcacgccg cgacgcgcgg
      781 cagggacgag gagcacgacg ccgcgccgcg ccgcgcgggg ggggggaggg agaggcagga
      841 cgcgggagcg agcgtgcatg tttccgcgcg agacgacgcc gcgcgcgctg gagaggagat
      901 aaggcgcttg gatcgcgaga gggccagcca ggctggaggc gaaaatgggt ggagaggata
      961 gtatcttgcg tgcttggacg aggagactga cgaggaggac ggatacgtcg atgatgatgt
     1021 gcacagagaa gaagcagttc gaaagcgact actagcaagc aagggatcca tgaaggctaa
     1081 cctcctcgtt cttctttccg ctctcgctgc tgcggatgcc gacaccatct gcattggcta
     1141 ccacgctaac aacagcacgg acaccgtcga tactgtcctg gagaagaacg ttaccgtcac
     1201 ccattcggtc aacctcctgg aggacagcca caacggcaag ctctgccgtc ttaagggcat
     1261 cgcccccctc cagctcggca agtgcaacat cgccggctgg ctcctcggca acccggagtg
     1321 cgatcccctc ctccccgttc gctcctggtc gtacattgtg gagactccga acagcgagaa
     1381 cggtatctgc taccccggcg attttatcga ctacgaggag ctccgcgagc agctctcctc
     1441 cgtgtccagc tttgagcgtt tcgagatttt tccgaaggag tcctcgtggc ccaaccacaa
     1501 caccaacggc gtcaccgccg cctgctccca cgagggcaag tcgagctttt accgcaacct
     1561 gctttggctc accgagaagg agggttcgta ccctaagctc aagaactcgt acgtcaacaa
     1621 gaagggcaag gaggtcctcg tcctctgggg catccaccat cccccgaaca gcaaggagca
     1681 gcagaacatc taccagaacg agaacgccta cgtttcggtg gtcacgtcga actacaaccg
     1741 ccgcttcact cctgagatcg ccgagcgccc caaggtgcgc gaccaggctg gccgcatgaa
     1801 ctactactgg accctcctta agcccggtga cacgatcatc tttgaggcca acggcaacct
     1861 tatcgcgccc atgtacgcgt tcgccctctc ccgcggcttt ggtagcggca tcattaccag
     1921 caacgccagc atgcacgagt gcaacacgaa gtgccagacc ccgctcggtg ccatcaacag
     1981 cagcctgcct taccagaaca tccaccccgt caccatcggt gagtgcccga agtacgtgcg
     2041 ctcggccaag ctccgcatgg tcacgggcct ccgcaacact ccttcgatcc agtccagcgg
     2101 cctcttcggc gccattgccg gtttcatcga gggcggctgg acgggcatga tcgacggctg
     2161 gtacggctac caccaccaga acgagcaggg ctccggttac gccgcggacc agaagtccac
     2221 gcagaacgcc atcaacggca ttactaacaa ggtcaacacg gtcatcgaga agatgaacat
     2281 tcagtttacc gctgtcggca aggagttcaa caagctggag aagcgcatgg agaacctcaa
     2341 caagaaggtg gacgatggtt tcctggacat ttggacctac aacgccgagc tcctcgtgct
     2401 ccttgagaac gagcgtaccc tcgacttcca cgactccaac gtcaagaacc tctacgagaa
     2461 ggtcaagtcg cagctcaaga acaacgccaa ggagattggc aacggttgct tcgagtttta
     2521 ccacaagtgc gacaacgagt gcatggagtc cgtccgcaac ggcacctacg actacccgaa
     2581 gtactccgag gagtcgaagc tgaaccgcga gaaggtggac ggcgtgaagc tggagtccat
     2641 gggcatctac cagatcctcg ccatttactc gacggttgcc tcgtcgctcg tcctccttgt
     2701 ctccctcggt gcgatttcgt tctggatgtg ctcgaacggc agccttcagt gccgcatctg
     2761 catctaacat atgagttatg agatccgaaa gtgaaccttg tcctaacccg acagcgaatg
     2821 gcgggagggg gcgggctaaa agatcgtatt acatagtatt tttcccctac tctttgtgtt
     2881 tgtctttttt ttttttttga acgcattcaa gccacttgtc tgggtttact tgtttgtttg
     2941 cttgcttgct tgcttgcttg cctgcttctt ggtcagacgg cccaaaaaag ggaaaaaatt
     3001 cattcatggc acagataaga aaaagaaaaa gtttgtcgac caccgtcatc agaaagcaag
     3061 agaagagaaa cactcgcgct cacattctcg ctcgcgtaag aatcttagcc acgcatacga
     3121 agtaatttgt ccatctggcg aatctttaca tgagcgtttt caagctggag cgtgagatca
     3181 tacctttctt gatcgtaatg ttccaacctt gcataggcct cgttgcgatc cgctagcaat
     3241 gcgtcgtact cccgttgcaa ctgcgccatc gcctcattgt gacgtgagtt cagattcttc
     3301 tcgagacctt cgagcgctgc taatttcgcc tgacgctcct tcttttgtgc ttccatgaca
     3361 cgccgcttca ccgtgcgttc cacttcttcc tcagacatgc ccttggctgc ctcgacctgc
     3421 tcggtaaaac gggccccagc acgtgctacg agatttcgat tccaccgccg ccttctatga
     3481 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     3541 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     3601 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     3661 tggtttgtcc aaactcatca atgtatctta tcatacatgg tcgacctgca ggaacctgca
     3721 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     3781 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     3841 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     3901 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     3961 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     4021 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     4081 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     4141 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     4201 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     4261 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     4321 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     4381 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     4441 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     4501 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     4561 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     4621 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     4681 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     4741 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     4801 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     4861 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     4921 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     4981 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     5041 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     5101 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     5161 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     5221 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     5281 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     5341 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     5401 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     5461 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     5521 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     5581 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     5641 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     5701 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     5761 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     5821 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     5881 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     5941 actgagagtg caccaagctt ccaattttag gccccccact gaccgaggtc tgtcgataat
     6001 ccacttttcc attgattttc caggtttcgt taactcatgc cactgagcaa aacttcggtc
     6061 tttcctaaca aaagctctcc tcacaaagca tggcgcggca acggacgtgt cctcatactc
     6121 cactgccaca caaggtcgat aaactaagct cctcacaaat agaggagaat tccactgaca
     6181 actgaaaaca atgtatgaga gacgatcacc actggagcgg cgcggcggtt gggcgcggag
     6241 gtcggcagca aaaacaagcg actcgccgag caaacccgaa tcagccttca gacggtcgtg
     6301 cctaacaaca cgccgttcta ccccgccttc ttcgcgcccc ttcgcgtcca agcatccttc
     6361 aagtttatct ctctagttca acttcaagaa gaacaacacc accaacacca tgattgaaca
     6421 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     6481 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     6541 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     6601 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     6661 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     6721 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     6781 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     6841 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     6901 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgatgatct
     6961 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     7021 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     7081 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     7141 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     7201 ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     7261 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     7321 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7381 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7441 catcaatgta tcttatcatg tctgaattcc cggggtac
//
