LOCUS       V008472                 7538 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008472
VERSION     V008472
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7538)
  AUTHORS   Bayne AC, Boltz D, Owen C, Betz Y, Maia G, Azadi P, Archer-Hartmann
            S, Zirkle R, Lippmeier JC.
  TITLE     Vaccination against Influenza with Recombinant Hemagglutinin
            Expressed by Schizochytrium sp. Confers Protective Immunity
  JOURNAL   PLoS ONE 8 (4), E61790 (2013)
   PUBMED   23626728
REFERENCE   2  (bases 1 to 7538)
  AUTHORS   Bayne A-C.V., Lippmeier JC.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-NOV-2012) Molecular Biology, DSM Nutritional Products,
            6480 Dobbin Rd., Columbia, MD 21045, USA
REFERENCE   3  (bases 1 to 7538)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7538)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2013"; volume: "8"; issue: "4"; pages: "E61790"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-NOV-2012) Molecular Biology, DSM Nutritional Products, 6480
            Dobbin Rd., Columbia, MD 21045, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..1064
                     /label="elongation factor 1 promoter"
                     /note="elongation factor 1 promoter"
                     /regulatory_class="promoter"
     CDS             1070..2824
                     /gene="HA"
                     /label="Hemagglutinin"
                     /note="Hemagglutinin from Influenza B virus (strain
                     B/Beijing/1/1987). Accession#: P17504"
     regulatory      2847..3486
                     /label="Pfa 3 terminator"
                     /note="Pfa 3 terminator"
                     /regulatory_class="terminator"
     polyA_signal    3629..3750
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4012..4600)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4774..5631)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5632..5736)
                     /label="AmpR promoter"
     regulatory      6021..6469
                     /label="alpha-tubulin promoter"
                     /note="alpha-tubulin promoter"
                     /regulatory_class="promoter"
     CDS             6473..7261
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"
     polyA_signal    7394..7527
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ctcttatctg cctcgcgccg ttgaccgccg cttgactctt ggcgcttgcc gctcgcatcc
       61 tgcctcgctc gcgcaggcgg gcgggcgagt gggtgggtcc gcagccttcc gcgctcgccc
      121 gctagctcgc tcgcgccgtg ctgcagccag cagggcagca ccgcacggca ggcaggtccc
      181 ggcgcggatc gatcgatcca tcgatccatc gatccatcga tcgtgcggtc aaaaagaaag
      241 gaagaagaaa ggaaaaagaa aggcgtgcgc acccgagtgc gcgctgagcg cccgctcgcg
      301 gtcccgcgga gcctccgcgt tagtccccgc cccgcgccgc gcagtccccc gggaggcatc
      361 gcgcacctct cgccgccccc tcgcgcctcg ccgattcccc gcctcccctt ttccgcttct
      421 tcgccgcctc cgctcgcggc cgcgtcgccc gcgccccgct ccctatctgc tccccagggg
      481 ggcactccgc accttttgcg cccgctgccg ccgccgcggc cgccccgccg ccctggtttc
      541 ccccgcgagc gcggccgcgt cgccgcgcaa agactcgccg cgtgccgccc cgagcaacgg
      601 gtggcggcgg cgcggcggcg ggcggggcgc ggcggcgcgt aggcggggct aggcgccggc
      661 taggcgaaac gccgcccccg ggcgccgccg ccgcccgctc cagagcagtc gccgcgccag
      721 accgccaacg cagagaccga gaccgaggta cgtcgcgccc gagcacgccg cgacgcgcgg
      781 cagggacgag gagcacgacg ccgcgccgcg ccgcgcgggg ggggggaggg agaggcagga
      841 cgcgggagcg agcgtgcatg tttccgcgcg agacgacgcc gcgcgcgctg gagaggagat
      901 aaggcgcttg gatcgcgaga gggccagcca ggctggaggc gaaaatgggt ggagaggata
      961 gtatcttgcg tgcttggacg aggagactga cgaggaggac ggatacgtcg atgatgatgt
     1021 gcacagagaa gaagcagttc gaaagcgact actagcaagc aagggatcca tgaaggctat
     1081 catcgttctc ctcatggtcg tcacctccaa cgctgatcgt atctgcaccg gcatcacctc
     1141 ctcgaactcc ccccacgtcg tcaagactgc tactcagggc gaggtcaacg tcaccggcgt
     1201 gattcccctc accaccactc ccaccaagtc ccactttgcc aacctcaagg gcactgagac
     1261 ccgcggaaag ctctgcccta agtgcctcaa ctgcaccgac ctggacgtcg ctctcggccg
     1321 ccccaagtgc accggcaaca ttccctccgc ccgcgtctcc attctccacg aggtccgccc
     1381 tgtcacttcc ggttgcttcc ccattatgca tgatcgcacc aagattcgtc agctccccaa
     1441 cctcctccgc ggctacgagc acattcgcct ctccacccac aacgtcatta acgccgagaa
     1501 cgcccccggc ggcccctaca agattggcac gtccggttcc tgccccaacg tcaccaacgg
     1561 caacggcttt tttgctacta tggcctgggc tgtccctaag aacgacaaca acaagactgc
     1621 caccaactcg ctcaccattg aggttcccta catctgcacc gagggcgagg accagatcac
     1681 tgtttggggt ttccacagcg acaacgagat tcagatggct aagctctacg gcgacagcaa
     1741 gccccagaag ttcacctcgt ccgccaacgg cgtcaccacc cactacgttt cccagatcgg
     1801 cggttttccc aaccagaccg aggacggcgg tctccctcag agcggccgca tcgtcgtcga
     1861 ctacatggtc cagaagtccg gcaagaccgg caccatcacg taccagcgcg gcatcctcct
     1921 cccccagaag gtttggtgcg cctccggtcg ttccaaggtt atcaagggtt cgctccccct
     1981 catcggagag gccgactgcc tgcacgagaa gtacggcgga ctcaacaagt cgaagcccta
     2041 ctacaccggt gagcacgcta aggccattgg caactgccct atttgggtca agacccccct
     2101 caagctcgct aacggaacta agtaccgtcc ccccgccaag ctgctcaagg agcgcggatt
     2161 ttttggcgcc atcgccggtt ttcttgaggg tggatgggag ggcatgatcg ctggctggca
     2221 cggttacacg tcccacggcg cgcatggagt cgctgtcgcc gctgacctta agagcaccca
     2281 ggaggccatt aacaagatca ccaagaacct caactccctt tccgagctcg aggttaagaa
     2341 ccttcagcgc ctctccggag ccatggacga gcttcacaac gagatccttg agctggacga
     2401 gaaggtcgac gacctccgcg cggacactat ctcctcccag attgagctgg ctgtccttct
     2461 ttccaacgag ggtatcatta actccgagga cgagcacctc ctcgcgctcg agcgtaagct
     2521 gaagaagatg ctcggaccta gcgccgtcga gattggtaac ggctgcttcg agactaagca
     2581 caagtgcaac cagacctgcc tcgaccgcat tgccgccggc accttcgacg ctggagagtt
     2641 ttcccttccc acctttgact cgctcaacat taccgccgcc tcgctcaacg acgacggtct
     2701 cgacaaccac accattctcc tctactactc caccgctgct tcctccctcg ccgtcaccct
     2761 catgattgcc attttcgtcg tctacatggt ttcccgcgac aacgtctcgt gctccatctg
     2821 cctctaacat atgagttatg agatccgaaa gtgaaccttg tcctaacccg acagcgaatg
     2881 gcgggagggg gcgggctaaa agatcgtatt acatagtatt tttcccctac tctttgtgtt
     2941 tgtctttttt ttttttttga acgcattcaa gccacttgtc tgggtttact tgtttgtttg
     3001 cttgcttgct tgcttgcttg cctgcttctt ggtcagacgg cccaaaaaag ggaaaaaatt
     3061 cattcatggc acagataaga aaaagaaaaa gtttgtcgac caccgtcatc agaaagcaag
     3121 agaagagaaa cactcgcgct cacattctcg ctcgcgtaag aatcttagcc acgcatacga
     3181 agtaatttgt ccatctggcg aatctttaca tgagcgtttt caagctggag cgtgagatca
     3241 tacctttctt gatcgtaatg ttccaacctt gcataggcct cgttgcgatc cgctagcaat
     3301 gcgtcgtact cccgttgcaa ctgcgccatc gcctcattgt gacgtgagtt cagattcttc
     3361 tcgagacctt cgagcgctgc taatttcgcc tgacgctcct tcttttgtgc ttccatgaca
     3421 cgccgcttca ccgtgcgttc cacttcttcc tcagacatgc ccttggctgc ctcgacctgc
     3481 tcggtaaaac gggccccagc acgtgctacg agatttcgat tccaccgccg ccttctatga
     3541 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     3601 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     3661 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     3721 tggtttgtcc aaactcatca atgtatctta tcatacatgg tcgacctgca ggaacctgca
     3781 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     3841 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     3901 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     3961 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     4021 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     4081 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     4141 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     4201 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     4261 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     4321 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     4381 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     4441 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     4501 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     4561 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     4621 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     4681 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     4741 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     4801 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     4861 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     4921 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     4981 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     5041 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     5101 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     5161 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     5221 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     5281 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     5341 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     5401 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     5461 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     5521 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     5581 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     5641 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     5701 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     5761 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     5821 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     5881 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     5941 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     6001 actgagagtg caccaagctt ccaattttag gccccccact gaccgaggtc tgtcgataat
     6061 ccacttttcc attgattttc caggtttcgt taactcatgc cactgagcaa aacttcggtc
     6121 tttcctaaca aaagctctcc tcacaaagca tggcgcggca acggacgtgt cctcatactc
     6181 cactgccaca caaggtcgat aaactaagct cctcacaaat agaggagaat tccactgaca
     6241 actgaaaaca atgtatgaga gacgatcacc actggagcgg cgcggcggtt gggcgcggag
     6301 gtcggcagca aaaacaagcg actcgccgag caaacccgaa tcagccttca gacggtcgtg
     6361 cctaacaaca cgccgttcta ccccgccttc ttcgcgcccc ttcgcgtcca agcatccttc
     6421 aagtttatct ctctagttca acttcaagaa gaacaacacc accaacacca tgattgaaca
     6481 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     6541 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     6601 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     6661 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     6721 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     6781 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     6841 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     6901 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     6961 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgatgatct
     7021 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     7081 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     7141 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     7201 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     7261 ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     7321 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     7381 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7441 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7501 catcaatgta tcttatcatg tctgaattcc cggggtac
//
