LOCUS       Exported               12341 bp DNA     circular SYN 08-JUL-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pCL-Eco
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12341)
  AUTHORS   Naviaux RK, Costanzi E, Haas M, Verma IM
  TITLE     The pCL vector system: rapid production of helper-free, high-titer, 
            recombinant retroviruses.
  JOURNAL   J Virol. 1996 Aug . 70(8):5701-5.
  PUBMED    8764092
REFERENCE   2  (bases 1 to 12341)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 12341)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            1996 Aug . 70(8):5701-5."
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     133..152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     386..403
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      468..602
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(521..540)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    1060..1194
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1084..1103)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1138..1157
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1466..1486)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          1616..1681
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(2394..4391)
                     /codon_start=1
                     /product="envelope glycoprotein"
                     /label=ENV
                     /translation="MARSTLSKPLKNKVNPRGPLIPLILLMLRGVSTASPGSSPHQVYN
                     ITWEVTNGDRETVWATSGNHPLWTWWPDLTPDLCMLAHHGPSYWGLEYQSPFSSPPGPP
                     CCSGGSSPGCSRDCEEPLTSLTPRCNTAWNRLKLDQTTHKSNEGFYVCPGPHRPRESKS
                     CGGPDSFYCAYWGCETTGRAYWKPSSSWDFITVNNNLTSDQAVQVCKDNKWCNPLVIRF
                     TDAGRRVTSWTTGHYWGLRLYVSGQDPGLTFGIRLRYQNLGPRVPIGPNPVLADQQPLS
                     KPKPVKSPSVTKPPSGTPLSPTQLPPAGTENRLLNLVDGAYQALNLTSPDKTQECWLCL
                     VAGPPYYEGVAVLGTYSNHTSAPANCSVASQHKLTLSEVTGQGLCIGAVPKTHQALCNT
                     TQTSSRGSYYLVAPTGTMWACSTGLTPCISTTILNLTTDYCVLVELWPRVTYHSPSYVY
                     GLFERSNRHKREPVSLTLALLLGGLTMGGIAAGIGTGTTALMATQQFQQLQAAVQDDLR
                     EVEKSISNLEKSLTSLSEVVLQNRRGLDLLFLKEGGLCAALKEECCFYADHTGLVRDSM
                     AKLRERLNQRQKLFESTQGWFEGLFNRSPWFTTLISTIMGPLIVLLMILLFGPCILNRL
                     VQFVKDRISVVQALVLTQQYHQLKPIEYEP"
     CDS             complement(4331..9547)
                     /codon_start=1
                     /gene="gag-pol"
                     /product="Moloney murine leukemia virus Gag-Pol
                     polyprotein"
                     /label=gag-pol
                     /note="contains a read-through stop codon"
                     /translation="MGQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPT
                     FNVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPK
                     PPPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGAKPKPQVLSDSGGPLIDLLTEDPP
                     PYRDPRPPPSDRDGNGGEATPAGEAPDPSPMASRLRGRREPPVADSTTSQAFPLRAGGN
                     GQLQYWPFSSSDLYNWKNNNPSFSEDPGKLTALIESVLITHQPTWDDCQQLLGTLLTGE
                     EKQRVLLEARKAVRGDDGRPTQLPNEVDAAFPLERPDWDYTTQAGRNHLVHYRQLLLAG
                     LQNAGRSPTNLAKVKGITQGPNESPSAFLERLKEAYRRYTPYDPEDPGQETNVSMSFIW
                     QSAPDIGRKLERLEDLKNKTLGDLVREAEKIFNKRETPEEREERIRRETEEKEERRRTE
                     DEQKEKERDRRRHREMSKLLATVVSGQKQDRQGGERRRSQLDRDQCAYCKEKGHWAKDC
                     PKKPRGPRGPRPQTSLLTLDD*GGQGQEPPPEPRITLKVGGQPVTFLVDTGAQHSVLTQ
                     NPGPLSDKSAWVQGATGGKRYRWTTDRKVHLATGKVTHSFLHVPDCPYPLLGRDLLTKL
                     KAQIHFEGSGAQVMGPMGQPLQVLTLNIEDEYRLHETSKEPDVSLGSTWLSDFPQAWAE
                     TGGMGLAVRQAPLIIPLKATSTPVSIKQYPMSQEARLGIKPHIQRLLDQGILVPCQSPW
                     NTPLLPVKKPGTNDYRPVQDLREVNKRVEDIHPTVPNPYNLLSGLPPSHQWYTVLDLKD
                     AFFCLRLHPTSQPLFAFEWRDPEMGISGQLTWTRLPQGFKNSPTLFDEALHRDLADFRI
                     QHPDLILLQYVDDLLLAATSELDCQQGTRALLQTLGNLGYRASAKKAQICQKQVKYLGY
                     LLKEGQRWLTEARKETVMGQPTPKTPRQLREFLGTAGFCRLWIPGFAEMAAPLYPLTKT
                     GTLFNWGPDQQKAYQEIKQALLTAPALGLPDLTKPFELFVDEKQGYAKGVLTQKLGPWR
                     RPVAYLSKKLDPVAAGWPPCLRMVAAIAVLTKDAGKLTMGQPLVILAPHAVEALVKQPP
                     DRWLSNARMTHYQALLLDTDRVQFGPVVALNPATLLPLPEEGLQHNCLDILAEAHGTRP
                     DLTDQPLPDADHTWYTDGSSLLQEGQRKAGAAVTTETEVIWAKALPAGTSAQRAELIAL
                     TQALKMAEGKKLNVYTDSRYAFATAHIHGEIYRRRGLLTSEGKEIKNKDEILALLKALF
                     LPKRLSIIHCPGHQKGHSAEARGNRMADQAARKAAITETPDTSTLLIENSSPYTSEHFH
                     YTVTDIKDLTKLGAIYDKTKKYWVYQGKPVMPDQFTFELLDFLHQLTHLSFSKMKALLE
                     RSHSPYYMLNRDRTLKNITETCKACAQVNASKSAVKQGTRVRGHRPGTHWEIDFTEIKP
                     GLYGYKYLLVFIDTFSGWIEAFPTKKETAKVVTKKLLEEIFPRFGMPQVLGTDNGPAFV
                     SKVSQTVADLLGIDWKLHCAYRPQSSGQVERMNRTIKETLTKLTLATGSRDWVLLLPLA
                     LYRARNTPGPHGLTPYEILYGAPPPLVNFPDPDMTRVTNSPSLQAHLQALYLVQHEVWR
                     PLAAAYQEQLDRPVVPHPYRVGDTVWVRRHQTKNLEPRWKGPYTVLLTTPTALKVDGIA
                     AWIHAAHVKAADPGGGPSSRLTWRVQRSQNPLKIRLTREAP"
     misc_feature    4394..4768
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     complement(4440..4456)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral
                     vector"
     primer_bind     complement(9144..9160)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE
                     vectors"
     primer_bind     complement(9176..9198)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called
                     MSCV"
     promoter        complement(9856..10059)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     primer_bind     complement(9885..9905)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        10060..10439
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(10584..10603)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     primer_bind     complement(10763..10781)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
     promoter        10849..10953
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10954..11814
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11172..11191)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(11985..12341,1..232)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
       61 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      121 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      181 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      241 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      301 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      361 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
      421 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagcc tccaaaaaag
      481 cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc tgcataaata
      541 aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg
      601 atgggcggag taagacccgc acccgttccc aaactgctta ttaaaggggg tatggaggtg
      661 ctggaccttg tgacagggcc agacagtgtg acagaaatag aagcagtcct gttttgacaa
      721 gttgcctctg gaagcctcta caatgcctct cttctttttc tccagagtaa gcggaggcca
      781 ggggcccccg gcctctgctt aatactaaaa aaaacagctg ttgtcatagt aatgattggg
      841 tggaaacatt ctaggcctgg gtggagaggc tttttgcttc ctcttgcaaa accacactgc
      901 cctctggagg gcagttgcct agcaactaat taaaagagga tgtcgcacgg ccagctgcgg
      961 tcagttagtc acttcctgct taactgactt gacattttct attttaagag tcgggaggaa
     1021 aattactgtg ttggaggccc tccgccatct tctgaagctg atccagacat gataagatac
     1081 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     1141 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     1201 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
     1261 aagtaaaacc tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca
     1321 tcaaatattt ttccataatt ttcttgtata gcagtgcagc tttttccttt gtggtgtaaa
     1381 tagcaaagca agcaagagtt ctattactaa acacagcatg actcaaaaaa cttagcaatt
     1441 ctgaaggaaa gtccttgggg tcttctacct ttctcttctt ttttggagga gtagaatgtt
     1501 gagagtcagc agtagcctca tcatcactag atggcatttc ttctgagcaa aacaggtttt
     1561 cctcattaaa ggcattccac cactgctccc attcatcagt tccataggtt ggaatctaaa
     1621 atacacaaac aattagaatc agtagtttaa cacattatac acttaaaaat tttatattta
     1681 ccttagagct ttaaatctct gtaggtagtt tgtccaatta tgtcacacca cagaagtaag
     1741 gttccttcac aaagatcctc tagctaccta gctagataca ctccgctatc gctacgtgac
     1801 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     1861 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     1921 aggttttcac cgtcatcacc gaaacgcgcg aggcagccag cttacctccc ggtggtgggt
     1981 cggtggtccc tgggcagggg tctcccgatc ccggacgagc ccccaaatga aagacccccg
     2041 ctgacgggta gtcaatcact cagaggagac cctcccaagg aacagcgaga ccacaagtcg
     2101 gatgcaactg caagagggtt tattggatac acgggtaccc gggcgactca gtcaatcgga
     2161 ggactggcgc cccgagtgag gggttgtggg ctcttttatt gagctcgggg agcagaagcg
     2221 cgcgaacaga agcgagaagc gaactgattg gttagttcaa ataaggcaca gggtcatttc
     2281 aggtccttgg ggcaccctgg aaacatctga tggttctcta gcttgccaaa cctacaggtg
     2341 gggtctttca ttcccccctt tttctggaga ctaaataaaa tcttttattt tatctatggc
     2401 tcgtactcta taggcttcag ctggtgatat tgttgagtca aaactagagc ctggaccact
     2461 gatatcctgt ctttaacaaa ttggactaat cgattaagaa tgcagggtcc gaagagcaaa
     2521 atcattagga gtacaatgag gggtcccata atggtagata tcaaggtggt aaaccaaggg
     2581 gatctgttaa acagtccctc aaaccatcct tgagttgact caaacagttt ctgtctctga
     2641 ttaagcctct ctctcaattt ggccatgctg tctctcacta gtcctgtgtg gtccgcatag
     2701 aagcaacatt cttcttttag agcagcacac agccctcctt cttttagaaa taacaagtct
     2761 aggccccttc gattctgtag gacaacttca gacagggaag tgagagactt ttctaggtta
     2821 gagattgatt tttcaacctc cctgagatca tcctgtactg cggcttggag ctgctggaat
     2881 tgctgagtgg ccattagagc agtagtccct gttcctattc cagcggcaat tccccccatg
     2941 gttagtccac ccaataatag ggccagggtt aacgacaccg gttctctttt gtgtcggttg
     3001 gatctctcaa acaggccgta aacatagctg ggggaatgat aggtgactct tggccagagt
     3061 tcgacaagaa cacaataatc agtggtaagg ttcagtatgg tggtggagat gcatggagta
     3121 agcccggtac tacaagccca catggtacct gtaggggcaa ctagataata ggaccctcga
     3181 ctgcttgtct gggtggtatt acatagggcc tgatgtgttt tgggaactgc tcctatgcag
     3241 agtccctgtc cggtcacttc ggacagggtc aacttgtgtt gggaggccac ggagcagttg
     3301 gctggagcag aggtatggtt ggagtaggta cccaggacgg caaccccttc gtagtagggg
     3361 ggtcccgcta ctagacacaa ccagcactct tgggttttgt caggactggt gaggttgagg
     3421 gcttggtagg ctccgtctac taagtttagc agcctatttt ccgttcccgc cggtggaagt
     3481 tgggtagggg agagaggagt cccactgggt ggtttggtga ctgaaggcga cttaacaggt
     3541 ttgggcttgg agagtggctg ttggtctgcc agaacggggt ttggccctat tgggacgcgg
     3601 ggtcctagat tttggtatct gagtcggatc ccaaatgtaa gccctggatc ttgtccggag
     3661 acatacaaac gtaagcccca gtaatgtcct gtggtccagg aagtaacccg tctcccggcg
     3721 tctgtaaacc gaataactaa ggggttgcac cacttattat ctttgcatac ctggacagcc
     3781 tggtcagagg tgagattgtt gtttactgtg atgaaatccc atgatgagga gggcttccag
     3841 taagctctac cggttgtctc acagccccaa taggcacagt agaaggagtc tggaccccca
     3901 catgacttgg attctcgggg gcggtggggc ccggggcaaa cataaaatcc ctcatttgat
     3961 ttatgagttg tctggtctag cttgagtctg ttccaggcag tgttgcaccg aggggtgagg
     4021 gaggttaaag gttcttcgca gtctctggaa cagcctgggc tgctgccccc tgagcaacaa
     4081 gggggccccg ggggagaaga aaaaggggat tgatattcta gcccccaata agatggtcca
     4141 tggtgggcta acatacataa atctggggta aggtcaggcc accaggtcca cagagggtgg
     4201 ttgccagaag ttgcccatac cgtctcccga tctccattgg ttacctccca ggtgatatta
     4261 tagacttgat gaggactgga gccgggcgaa gcagtactga cccctctgag catcagaaga
     4321 attaagggga ttagggggcc tcgcgggtta accttatttt taaggggttt tgagagcgtt
     4381 gaacgcgcca tgtcagtcta gaggatggtc cacccccggg gtcggcagcc ttcacgtggg
     4441 cggcgtgtat ccaagctgcg atgccgtcta ctttgagggc ggtgggggtg gtcagcagga
     4501 ctgtgtaagg tcctttccag cgaggttcta ggttcttagt ctggtgtcgg cggacccaca
     4561 ctgtgtcgcc gactcggtaa gggtgaggta ccaccggtcg gtccagttgt tcttggtagg
     4621 ctgccgccag aggtctccag acttcgtgct ggactaagta gagagcctgt aagtgagctt
     4681 ggagagaggg gctgttagta actcttgtca tgtcagggtc agggaagttt acaaggggcg
     4741 ggggtgcccc atataagatc tcatatgggg tgaggccatg ggggcccggc gtgttgcggg
     4801 ctcggtacag ggctaagggg agtaggagca cccagtctct agagccagtt gcaagcgtta
     4861 atttagttaa agtctccttg atggttctat tcattctttc tacctggcct gagctttggg
     4921 gtctgtatgc acaatgtaat ttccaatcaa tccccaacag atcggccact gtctgactca
     4981 ccttggagac gaaggcaggc ccattgtcag ttcccaatac ctgaggcatg ccgaacctgg
     5041 ggaagatctc ctctagtagc ttcttggtta cgaccttggc ggtttctttc ttggttggga
     5101 aggcttctat ccagccagaa aaggtatcta taaaaactag aagatattta tagccataca
     5161 atccgggctt tatctcggtg aaatcgatct cccaatgagt gccgggccga tgcccgcgga
     5221 ccctagttcc ctgtttaacg gcagacttgc tggcgttgac ttgtgcacaa gctttgcagg
     5281 tctcagtgat atttttgagt gttcgatccc ggttcagcat gtagtaggga ctgtggcttc
     5341 tctctaggag agccttcatt tttgagaagc tgaggtgagt cagctgatga agaaagtcta
     5401 ataattcaaa agtaaactgg tcaggcatca caggttttcc ttggtagacc caatacttct
     5461 ttgttttatc ataaatggcc cccaacttgg ttaggtcctt tatatcagtc actgtgtaat
     5521 gaaaatgttc tgaggtgtag ggtgatgaat tttctatgag gagggtagag gtgtctggag
     5581 tctctgtgat ggctgccttt cgggccgctt ggtcagccat ccggttgcct ctagcctcgg
     5641 cgctgtgtcc cttttgatgt cctggacaat ggattatgct aagtcttttg ggcagaaaga
     5701 gggcttttag tagggccaag atctcgtctt tatttttgat ctctttgcct tctgatgtga
     5761 gcaacccacg ccttctgtat atttctccat ggatatgggc agtagcaaaa gcataacggc
     5821 tatcagtata aacatttagc ttcttacctt ctgccatctt tagggcctgg gtgagtgcta
     5881 tcagttcagc ccgctgagcg gatgtcccgg ctggcagggc tttagcccag attacctcgg
     5941 tctcggtggt caccgcagct cccgccttac gctgtccctc ttgtaagaga ctgcttccat
     6001 ccgtgtacca ggtgtggtcg gcgtctggga gcggctggtc cgttaggtcg ggtcgggttc
     6061 cgtgggcttc ggccaggata tcaaggcagt tgtgttgcag cccttcctca ggcagtggga
     6121 gcagcgtagc cgggttcagg gctaccaccg gtccgaactg gacccggtcc gtgtccaaaa
     6181 gcaaggcctg atagtgagtc atccgggcgt tggaaagcca gcggtcgggg ggttgtttga
     6241 ctagtgcctc tactgcatgg ggggccagaa tgactagtgg ctgtcccatg gttagcttgc
     6301 ctgcatcctt tgtcagtacg gcaatggctg ctaccatccg taggcaaggg ggccacccag
     6361 ctgctactgg gtctagcttt ttggacaggt aggccaccgg ccgacgccaa ggtcccagtt
     6421 tttgcgttag gacacctttg gcgtagccct gcttctcgtc gacaaagagt tcaaagggct
     6481 tagtcaaatc tggcaacccc agggctgggg cagttagaag agcttgcttg atttcttgat
     6541 aggccttttg ttggtctggg ccccaattaa acagagtccc cgttttggtg agagggtaca
     6601 agggggctgc catttctgca aacccaggga tccagaggcg acagaagcct gccgtcccta
     6661 ggaactccct tagttgtcga ggggtcttcg gagtaggctg ccccatcaca gtctcttttc
     6721 tggcctcagt cagccatctc tgaccctctt ttagaagata ccccagatac ttgacctgtt
     6781 tctggcaaat ttgggctttc ttggccgagg cccgataccc gaggttccct agggtttgta
     6841 acagggcccg agtaccttgt tggcagtcta gctcagaagt ggcggccagc agtaagtcat
     6901 ccacgtactg tagcaggatc aagtctgggt gctggatccg gaagtctgct aggtctctgt
     6961 gcagtgcctc atcaaacagg gtgggactgt ttttgaaacc ctgtgggagt ctggtccagg
     7021 tcaattgtcc tgagattccc atctctggat ctctccactc aaaggcgaag agaggctgac
     7081 tggtggggtg gagtctcagg cagaaaaagg catcctttaa atcaagcaca gtgtaccact
     7141 ggtgggacgg tgggagcccg ctcaagaggt tgtaagggtt gggcacggtg gggtggatgt
     7201 cttccacccg cttgttgact tctctcagat cctggacagg cctataatca ttagtccctg
     7261 gtttcttaac gggtagcagg ggcgtgttcc agggggactg gcagggtacc agtattccct
     7321 ggtccaacag tctctgtatg tggggcttga tccccagtct ggcttcttgt gacatggggt
     7381 attgttttat ggacacgggg gtagaggttg ctttcagagg tatgatcaga ggagcttggc
     7441 gaactgccag tcccatgccc ccggtttccg cccaggcctg aggaaaatca gacagccatg
     7501 tggaccctag agaaacatct ggctcttttg aggtctcatg tagccgatac tcatcttcta
     7561 tatttagggt caacacttgc aggggctgcc ccattggtcc cataacctga gctcctgatc
     7621 cctcaaagtg gatttgggct tttagtttag tcagcaaatc tcttcctaac agaggatagg
     7681 gacagtctgg tacatggagg aaagagtggg tgaccttacc ggtagctaga tgtactttgc
     7741 gatccgtggt ccagcgatac cgctttcctc cagtagcccc ttggacccag gcagacttat
     7801 cacttagggg tccaggattt tgggtcagca cggagtgttg ggccccagta tctaccagga
     7861 aggtgacggg ttgccccccg actttgaggg ttatcctggg ttcagggggg ggctcctgac
     7921 cctgacctcc ctagtcatct agggtcagga gggaggtctg gggtcttggt ccccgaggtc
     7981 ctcgtggttt cttgggacaa tctttagccc agtgcccctt ttctttgcag taggcacact
     8041 ggtcgcgatc gagttgggac ctccttcgtt ctcctccctg tctatcctgt ttctgtccac
     8101 taacgacagt ggccaatagc ttgctcatct ctctatgtct cctacgatct ctttctttct
     8161 ctttctgctc atcctctgtc ctacggcgtt cttctttttc ctctgtttct ctcctgatac
     8221 gttcctctct ttcttccggg gtttctcgtt tattaaagat cttttctgcc tctctaacca
     8281 aatctccaag cgtcttgttt tttaaatctt ctaacctctc taactttctc ccaatgtctg
     8341 gggcagactg ccaaatgaaa gacatagaca cattagtttc ttgccctggg tcctcagggt
     8401 cataaggagt gtacctgcga taggcttcct taagtctctc taggaaggcc gagggagact
     8461 cattgggccc ttgtgttatt ccttttacct tggccaaatt ggtggggctt ctgcccgcgt
     8521 tttggagacc cgctaggagc aactggcgat agtggactag gtggttccta cctgcctggg
     8581 tggtgtaatc ccagtctggg cgctcgaggg gaaaagcggc atcgacttca ttgggcagtt
     8641 gagtggggcg cccatcatcg ccccgcaccg cctttctagc ctctaagagc acccgttgtt
     8701 tttcttctcc ggtcagcaga gtccccaaca gctgctgaca gtcgtcccag gtgggctgat
     8761 gggtgatgag gacagactcg atcagagctg tcagtttacc tggatcttca gaaaaagaag
     8821 ggttattatt tttccagttg taaaggtcag aagaggagaa cggccagtat tgaagctgtc
     8881 cgtttcctcc tgcgcggagg gggaatgcct gcgaggtagt ggagtcggcc acagggggct
     8941 cccgtctccc acgtaggcga gatgccattg gggaggggtc cggtgcctct cccgcagggg
     9001 tcgcttctcc accatttccg tccctgtcgg aagggggtgg tcttgggtcc ctataaggcg
     9061 gggggtcttc tgtaagtagg tcgatgagcg gccccccact gtcagaaaga acttgaggtt
     9121 taggtttggc gcctagagaa ggagtgaggg ctggataaag ggaggatcga ggcggggtcg
     9181 aacgaggagg ttcaaggggg agagacgggg cggatggagg aagaggaggc ggaggcttag
     9241 ggtgtacaaa gggcttgacc cagggagggg ggtcaaaagc caaggcttcc caggtcacga
     9301 tgtaggggac ctggtctggg tgtccatgcg ggccaggtga aaagaccttg atcttaacct
     9361 gggtgatgag gtctcggtta aaggtgccgt ctcgcggcca tccgacgtta aaggttggcc
     9421 attctgcaga gcagaaggta acccaacgtc tcttcttgac atctaccgac tggttgtgag
     9481 cgatccgctc gacatctttc cagtgaccta aggtcaaact taagggagtg gtaacagtct
     9541 ggcccatatt ttcagacaaa tacagaaaca cagtcagaca gagacaacac agaacgatgc
     9601 ccaagcttgg ccagcttacc tcccggtggt gggtcggtgg tccctgggca ggggtctccc
     9661 gatcccggac gagcccccaa atgaaagacc cccgctgacg ggtagtcaat cactcagagg
     9721 agaccctccc aaggaacagc gagaccacaa gtcggatgca actgcaagag ggtttattgg
     9781 atacacgggt acccgggcga ctcagtcaat cggaggactg gcgcgccgag tgaggggttg
     9841 tgggctcttt tattgagctc tgcttatata gacctcccac cgtacacgcc taccgcccat
     9901 ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca
     9961 aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg
    10021 ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa
    10081 tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca
    10141 ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg
    10201 atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt
    10261 ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt
    10321 gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg
    10381 gctatgaact aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtca
    10441 acgcgctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
    10501 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
    10561 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
    10621 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
    10681 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
    10741 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
    10801 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
    10861 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
    10921 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
    10981 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
    11041 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
    11101 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
    11161 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
    11221 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
    11281 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
    11341 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
    11401 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
    11461 gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc
    11521 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
    11581 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
    11641 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
    11701 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
    11761 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
    11821 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
    11881 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    11941 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    12001 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    12061 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    12121 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    12181 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    12241 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    12301 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa c
//
