LOCUS       40924_10916        8346 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCK911, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8346)
  AUTHORS   Kraft C.
  TITLE     A toolbox for the analysis of weak and low abundant molecule 
            interactions in vivo by M-Track in budding yeast
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8346)
  AUTHORS   Brezovich A, Schuschnig M, Ammerer G, Kraft C.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2014) Department of Biochemistry and Cell Biology,
            MFPL, University of Vienna, Dr. Bohr Gasse 9, Vienna 1030, Austria
REFERENCE   3  (bases 1 to 8346)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8346)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2014) Department of Biochemistry and Cell Biology, MFPL, 
            University of Vienna, Dr. Bohr Gasse 9, Vienna 1030, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        426..530
                     /label=AmpR promoter
     CDS             531..1388
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1562..2150
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2438..2459
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2474..2504
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2512..2528
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2536..2552
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2573..2591
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3360..4064
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     primer_bind     4078..4094
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4096..4419
                     /codon_start=1
                     /label=FKBP
                     /note="human FK506-binding protein FKBP12"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             4447..4476
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     terminator      5830..6077
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(6096..6114)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6124..6140)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      6285..6740
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(7003..7659)
                     /codon_start=1
                     /label=HIS3
                     /note="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL
                     ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(7660..7846)
                     /label=HIS3 promoter
     misc_feature    complement(join(8232..8346,1..389))
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
ORIGIN
        1 aaagaaggta gaagagttac ggaatgaaga aaaaaaaata aacaaaggtt taaaaaattt
       61 caacaaaaag cgtactttac atatatattt attagacaag aaaagcagat taaatagata
      121 tacattcgat taacgataag taaaatgtaa aatcacagga ttttcgtgtg tggtcttcta
      181 cacagacaag atgaaacaat tcggcattaa tacctgagag caggaagagc aagataaaag
      241 gtagtatttg ttggcgatcc ccctagagtc ttttacatct tcggaaaaca aaaactattt
      301 tttctttaat ttcttttttt actttctatt tttaatttat atatttatat taaaaaattt
      361 aaattataat tatttttata gcacgtgatg aaaaggaccc aggtggcact tttcggggaa
      421 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      481 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
      541 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
      601 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
      661 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
      721 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
      781 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
      841 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
      901 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
      961 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     1021 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     1081 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     1141 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     1201 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     1261 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     1321 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     1381 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     1441 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     1501 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1561 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1621 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1681 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     1741 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1801 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1861 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     1921 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     1981 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2041 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     2101 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     2161 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     2221 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     2281 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     2341 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     2401 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     2461 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     2521 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     2581 ctcactaaag ggaacaaaag ctggagctcg ccgggatcga agaaatgatg gtaaatgaaa
     2641 taggaaatca aggagcatga aggcaaaaga caaatataag ggtcgaacga aaaataaagt
     2701 gaaaagtgtt gatatgatgt atttggcttt gcggcgccga aaaaacgagt ttacgcaatt
     2761 gcacaatcat gctgactctg tggcggaccc gcgctcttgc cggcccggcg ataacgctgg
     2821 gcgtgaggct gtgcccggcg gagttttttg cgcctgcatt ttccaaggtt taccctgcgc
     2881 taaggggcga gattggagaa gcaataagaa tgccggttgg ggttgcgatg atgacgacca
     2941 cgacaactgg tgtcattatt taagttgccg aaagaacctg agtgcatttg caacatgagt
     3001 atactagaag aatgagccaa gacttgcgag acgcgagttt gccggtggtg cgaacaatag
     3061 agcgaccatg accttgaagg tgagacgcgc ataaccgcta gagtactttg aagaggaaac
     3121 agcaataggg ttgctaccag tataaataga caggtacata caacactgga aatggttgtc
     3181 tgtttgagta cgctttcaat tcatttgggt gtgcacttta ttatgttaca atatggaagg
     3241 gaactttaca cttctcctat gcacatatat taattaaagt ccaatgctag tagagaaggg
     3301 gggtaacacc cctccgcgct cttttccgat ttttttctaa accgtggaat atttcggata
     3361 tccttttgtt gtttccgggt gtacaatatg gacttcctct tttctggcaa ccaaacccat
     3421 acatcgggat tcctataata ccttcgttgg tctccctaac atgtaggtgg cggaggggag
     3481 atatacaata gaacagatac cagacaagac ataatgggct aaacaagact acaccaatta
     3541 cactgcctca ttgatggtgg tacataacga actaatactg tagccctaga cttgatagcc
     3601 atcatcatat cgaagtttca ctaccctttt tccatttgcc atctattgaa gtaataatag
     3661 gcgcatgcaa cttcttttct ttttttttct tttctctctc ccccgttgtt gtctcaccat
     3721 atccgcaatg acaaaaaaat gatggaagac actaaaggaa aaaattaacg acaaagacag
     3781 caccaacaga tgtcgttgtt ccagagctga tgaggggtat ctcgaagcac acgaaacttt
     3841 ttccttcctt cattcacgca cactactctc taatgagcaa cggtatacgg ccttccttcc
     3901 agttacttga atttgaaata aaaaaaagtt tgctgtcttg ctatcaagta taaatagacc
     3961 tgcaattatt aatcttttgt ttcctcgtca ttgttctcgt tccctttctt ccttgtttct
     4021 ttttctgcac aatatttcaa gctataccaa gcatacaatc aactccaagc tggccgctct
     4081 agaactagtg gatccatggg agtgcaggtg gaaaccatct ccccaggaga cgggcgcacc
     4141 ttccccaagc gcggccagac ctgcgtggtg cactacaccg ggatgcttga agatggaaag
     4201 aaatttgatt cctcccggga cagaaacaag ccctttaagt ttatgctagg caagcaggag
     4261 gtgatccgag gctgggaaga aggggttgcc cagatgagtg tgggtcagag agccaaactg
     4321 actatatctc cagattatgc ctatggtgcc actgggcacc caggcatcat cccaccacat
     4381 gccactctcg tcttcgatgt ggagcttcta aaactggaat ccctgcaggg ttctgctgct
     4441 agtggtgaac aaaagttgat ttctgaagaa gatttgaacg gtgaacaaaa gctaatctcc
     4501 gaggaagact tgaacggtga acaaaaatta atctcagaag aagacttgaa cggatcctct
     4561 agaggtgaac aaaagttgat ttctgaagaa gatttgaacg gtgaacaaaa gctaatctcc
     4621 gaggaagact tgaacggtga acaaaaatta atctcagaag aagacttgaa cggatcctct
     4681 agaggtgaac aaaagttgat ttctgaagaa gatttgaacg gtgaacaaaa gctaatctcc
     4741 gaggaagact tgaacggtga acaaaaatta atctcagaag aagacttgaa cggatccact
     4801 ctgcaggagt ctaggggatc ctgtgtgcgt atcctcaagc agttccacaa ggacttagaa
     4861 agggagctgc tccggcggca ccaccggtca aagacccccc ggcacctgga cccaagcttg
     4921 gccaactacc tggtgcagaa ggccaagcag aggcgggcgc tccgtcgctg ggagcaggag
     4981 ctcaatgcca agcgcagcca tctgggacgc atcactgtag agaatgaggt ggacctggac
     5041 ggccctcctc gggccttcgt gtacatcaat gagtaccgtg ttggtgaggg catcaccctc
     5101 aaccaggtgg ctgtgggctg cgagtgccag gactgtctgt gggcacccac tggaggctgc
     5161 tgcccggggg cgtcactgca caagtttgcc tacaatgacc agggccaggt gcggcttcga
     5221 gccgggctgc ccatctacga gtgcaactcc cgctgccgct gcggctatga ctgcccaaat
     5281 cgtgtggtac agaagggtat ccgatatgac ctctgcatct tccgcacgga tgatgggcgt
     5341 ggctggggcg tccgcaccct ggagaagatt cgcaagaaca gcttcgtcat ggagtacgtg
     5401 ggagagatca ttacctcaga ggaggcagag cggcggggcc agatctacga ccgtcagggc
     5461 gccacctacc tctttgacct ggactacgtg gaggacgtgt acaccgtgga tgccgcctac
     5521 tatggcaaca tctcccgctt tgtcaaccac agttgtgacc ccaacctaca ggtgtacaac
     5581 gtcttcatag acaaccttga cgagcggctg ccccgcatcg ctttctttgc cacaagaacc
     5641 atccgggcag gcgaggagct cacctttgat tacaacatgc aagtggaccc cgtggacatg
     5701 gagagcaccc gcatggactc caactttggc ctggctgggc tccctggctc ccctaagaag
     5761 cgggtccgta ttgaatgcaa gtgtgggact gagtcctgcc gcaaatacct cttctaggtc
     5821 gacctcgagt catgtaatta gttatgtcac gcttacattc acgccctccc cccacatccg
     5881 ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt tattttttta
     5941 tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt tttctgtaca
     6001 gacgcgtgta cgcatgtaac attatactga aaaccttgct tgagaaggtt ttgggacgct
     6061 cgaaggcttt aatttgcggc cggtacccaa ttcgccctat agtgagtcgt attacgcgcg
     6121 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     6181 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     6241 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcgacgcgc cctgtagcgg
     6301 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     6361 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     6421 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     6481 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     6541 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     6601 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     6661 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     6721 aatattaacg tttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     6781 tcacaccgca tagatccgtc gagttcaaga gaaaaaaaaa gaaaaagcaa aaagaaaaaa
     6841 ggaaagcgcg cctcgttcag aatgacacgt atagaatgat gcattacctt gtcatcttca
     6901 gtatcatact gttcgtatac atacttactg acattcatag gtatacatat atacacatgt
     6961 atatatatcg tatgctgcag ctttaaataa tcggtgtcac tacataagaa cacctttggt
     7021 ggagggaaca tcgttggtac cattgggcga ggtggcttct cttatggcaa ccgcaagagc
     7081 cttgaacgca ctctcactac ggtgatgatc attcttgcct cgcagacaat caacgtggag
     7141 ggtaattctg ctagcctctg caaagctttc aagaaaatgc gggatcatct cgcaagagag
     7201 atctcctact ttctcccttt gcaaaccaag ttcgacaact gcgtacggcc tgttcgaaag
     7261 atctaccacc gctctggaaa gtgcctcatc caaaggcgca aatcctgatc caaacctttt
     7321 tactccacgc gccagtaggg cctctttaaa agcttgaccg agagcaatcc cgcagtcttc
     7381 agtggtgtga tggtcgtcta tgtgtaagtc accaatgcac tcaacgatta gcgaccagcc
     7441 ggaatgcttg gccagagcat gtatcatatg gtccagaaac cctatacctg tgtggacgtt
     7501 aatcacttgc gattgtgtgg cctgttctgc tactgcttct gcctcttttt ctgggaagat
     7561 cgagtgctct atcgctaggg gaccaccctt taaagagatc gcaatctgaa tcttggtttc
     7621 atttgtaata cgctttacta gggctttctg ctctgtcatc tttgccttcg tttatcttgc
     7681 ctgctcattt tttagtatat tcttcgaaga aatcacatta ctttatataa tgtataattc
     7741 attatgtgat aatgccaatc gctaagaaaa aaaaagagtc atccgctagg ggaaaaaaaa
     7801 aaatgaaaat cattaccgag gcataaaaaa atatagagtg tactagagga ggccaagagt
     7861 aatagaaaaa gaaaattgcg ggaaaggact gtgttatgac ttccctgact aatgccgtgt
     7921 tcaaacgata cctggcagtg actcctagcg ctcaccaagc tcttaaaacg ggaatttatg
     7981 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     8041 aacacgcgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     8101 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     8161 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     8221 ttcttaggac ggatcgcttg cctgtaactt acacgcgcct cgtatctttt aatgatggaa
     8281 taatttggga atttactctg tgtttattta tttttatgtt ttgtatttgg attttagaaa
     8341 gtaaat
//
