LOCUS       40924_10911        6340 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCK909, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6340)
  AUTHORS   Kraft C.
  TITLE     A toolbox for the analysis of weak and low abundant molecule 
            interactions in vivo by M-Track in budding yeast
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6340)
  AUTHORS   Brezovich A, Schuschnig M, Ammerer G, Kraft C.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2014) Department of Biochemistry and Cell Biology,
            MFPL, University of Vienna, Dr. Bohr Gasse 9, Vienna 1030, Austria
REFERENCE   3  (bases 1 to 6340)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6340)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2014) Department of Biochemistry and Cell Biology, MFPL, 
            University of Vienna, Dr. Bohr Gasse 9, Vienna 1030, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    167..188
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        203..233
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    241..257
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     265..281
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        302..320
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        344..785
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     misc_feature    815..1108
                     /label=4x Histone3
                     /note="4x Histone3"
     CDS             1142..1315
                     /codon_start=1
                     /label=ProtA
                     /note="IgG-binding unit of Staphylococcus aureus protein A"
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             1496..1516
                     /codon_start=1
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
                     /translation="ENLYFQG"
     primer_bind     complement(1606..1622)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      1625..1872
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(1891..1909)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1919..1935)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2080..2535
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(2669..3469)
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(3470..3685)
                     /label=URA3 promoter
     misc_feature    complement(3955..4458)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     promoter        4495..4599
                     /label=AmpR promoter
     CDS             4600..5457
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5631..6219
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
       61 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
      121 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttacc
      181 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcctatg ttgtgtggaa
      241 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
      301 caattaaccc tcactaaagg gaacaaaagc tggagctcta gtacggatta gaagccgccg
      361 agcgggtgac agccctccga aggaagactc tcctccgtgc gtcctcgtct tcaccggtcg
      421 cgttcctgaa acgcagatgt gcctcgcgcc gcactgctcc gaacaataaa gattctacaa
      481 tactagcttt tatggttatg aagaggaaaa attggcagta acctggcccc acaaaccttc
      541 aaatgaacga atcaaattaa caaccatagg atgataatgc gattagtttt ttagccttat
      601 ttctggggta attaatcagc gaagcgatga tttttgatct attaacagat atataaatgc
      661 aaaaactgca taaccacttt aactaatact ttcaacattt tcggtttgta ttacttctta
      721 ttcaaatgta ataaaagtat caacaaaaaa ttgttaatat acctctatac tttaacgtca
      781 aggagaaaaa accccggatt ctagaactag tatggccaga accaagcaaa cagctaggaa
      841 atcaactggt ggtaaagcac ctcgtaaaca attagctcag ataccaaata gtgccagaac
      901 caaacaaact gcgaggaaaa gtacaggagg caaagctcct agaaaacaat tagcccaaat
      961 acccaattct gcaagaacaa agcaaacagc gagaaaatct accggtggta aggccccaag
     1021 aaagcagtta gcacaaattc caaactcggc tagaacgaaa caaacggcta gaaagtcaac
     1081 tggaggtaaa gcaccaagga aacagttgac tagctcttta ggggatggat ctcctaggat
     1141 ggttgacaac aaattcaaca aggaacaaca gaatgccttt tatgagattc tacatcttcc
     1201 gaacttgaac gaagagcaaa gaaatgcatt tatccagagc ttgaaagatg atccttccca
     1261 aagtgccaat ctacttgctg aagccaagaa attgaacgat gcacaagcac ctaaagtcga
     1321 taacaagttc aataaggaac agcaaaatgc tttctatgaa attctgcact tacccaatct
     1381 aaacgaagaa cagcgtaatg cttttatcca atcgttgaaa gacgatccat cccaatcagc
     1441 aaatcttctg gcagaagcga agaagttgaa tgatgctcaa gctccaaagg cttccgagaa
     1501 tttgtacttt caaggcgaat tcggcaaaat ctcatcctcc ggggcacttg attatgatat
     1561 tccaactact gctagcggcc tgcaggaatt cgatatcaag cttatcgata ccgtcgacct
     1621 cgagtcatgt aattagttat gtcacgctta cattcacgcc ctccccccac atccgctcta
     1681 accgaaaagg aaggagttag acaacctgaa gtctaggtcc ctatttattt ttttatagtt
     1741 atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct gtacagacgc
     1801 gtgtacgcat gtaacattat actgaaaacc ttgcttgaga aggttttggg acgctcgaag
     1861 gctttaattt gcggccggta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     1921 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     1981 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     2041 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga cgcgccctgt agcggcgcat
     2101 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     2161 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     2221 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     2281 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     2341 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     2401 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     2461 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     2521 taacgtttac aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     2581 ccgcataggg taataactga tataattaaa ttgaagctct aatttgtgag tttagtatac
     2641 atgcatttac ttataataca gttttttagt tttgctggcc gcatcttctc aaatatgctt
     2701 cccagcctgc ttttctgtaa cgttcaccct ctaccttagc atcccttccc tttgcaaata
     2761 gtcctcttcc aacaataata atgtcagatc ctgtagagac cacatcatcc acggttctat
     2821 actgttgacc caatgcgtct cccttgtcat ctaaacccac accgggtgtc ataatcaacc
     2881 aatcgtaacc ttcatctctt ccacccatgt ctctttgagc aataaagccg ataacaaaat
     2941 ctttgtcgct cttcgcaatg tcaacagtac ccttagtata ttctccagta gatagggagc
     3001 ccttgcatga caattctgct aacatcaaaa ggcctctagg ttcctttgtt acttcttctg
     3061 ccgcctgctt caaaccgcta acaatacctg ggcccaccac accgtgtgca ttcgtaatgt
     3121 ctgcccattc tgctattctg tatacacccg cagagtactg caatttgact gtattaccaa
     3181 tgtcagcaaa ttttctgtct tcgaagagta aaaaattgta cttggcggat aatgccttta
     3241 gcggcttaac tgtgccctcc atggaaaaat cagtcaagat atccacatgt gtttttagta
     3301 aacaaatttt gggacctaat gcttcaacta actccagtaa ttccttggtg gtacgaacat
     3361 ccaatgaagc acacaagttt gtttgctttt cgtgcatgat attaaatagc ttggcagcaa
     3421 caggactagg atgagtagca gcacgttcct tatatgtagc tttcgacatg atttatcttc
     3481 gtttcctgca tgtttttgtt ctgtgcagtt gggttaagaa tactgggcaa tttcatgttt
     3541 cttcaacact acatatgcgt atatatacca atctaagtct gtgctccttc cttcgttctt
     3601 ccttctgttc ggagattacc gaatcaaaaa aatttcaaag aaaccgaaat caaaaaaaag
     3661 aataaaaaaa aaatgatgaa ttgaattgaa aagctgtggt atggtgcact ctcagtacaa
     3721 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     3781 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     3841 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     3901 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag gacggatcgc
     3961 ttgcctgtaa cttacacgcg cctcgtatct tttaatgatg gaataatttg ggaatttact
     4021 ctgtgtttat ttatttttat gttttgtatt tggattttag aaagtaaata aagaaggtag
     4081 aagagttacg gaatgaagaa aaaaaaataa acaaaggttt aaaaaatttc aacaaaaagc
     4141 gtactttaca tatatattta ttagacaaga aaagcagatt aaatagatat acattcgatt
     4201 aacgataagt aaaatgtaaa atcacaggat tttcgtgtgt ggtcttctac acagacaaga
     4261 tgaaacaatt cggcattaat acctgagagc aggaagagca agataaaagg tagtatttgt
     4321 tggcgatccc cctagagtct tttacatctt cggaaaacaa aaactatttt ttctttaatt
     4381 tcttttttta ctttctattt ttaatttata tatttatatt aaaaaattta aattataatt
     4441 atttttatag cacgtgatga aaaggaccca ggtggcactt ttcggggaaa tgtgcgcgga
     4501 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4561 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4621 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4681 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     4741 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     4801 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     4861 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     4921 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     4981 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5041 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5101 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     5161 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5221 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5281 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     5341 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     5401 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     5461 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     5521 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     5581 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5641 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     5701 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5761 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     5821 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     5881 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5941 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6001 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6061 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6121 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6181 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6241 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6301 gattctgtgg ataaccgtat taccgccttt gagtgagctg
//
