LOCUS       40924_10901        8088 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCK907, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8088)
  AUTHORS   Kraft C.
  TITLE     A toolbox for the analysis of weak and low abundant molecule 
            interactions in vivo by M-Track in budding yeast
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8088)
  AUTHORS   Brezovich A, Schuschnig M, Ammerer G, Kraft C.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2014) Department of Biochemistry and Cell Biology,
            MFPL, University of Vienna, Dr. Bohr Gasse 9, Vienna 1030, Austria
REFERENCE   3  (bases 1 to 8088)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8088)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2014) Department of Biochemistry and Cell Biology, MFPL, 
            University of Vienna, Dr. Bohr Gasse 9, Vienna 1030, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(66..569)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     promoter        606..710
                     /label=AmpR promoter
     CDS             711..1568
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1742..2330
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2618..2639
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2654..2684
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2692..2708
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2716..2732
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2753..2771
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2795..3236
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     CDS             3287..3316
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             3323..3352
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3359..3388
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3407..3436
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3443..3472
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3479..3508
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3527..3556
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3563..3592
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3599..3628
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    3662..4654
                     /label=HKMT
                     /note="HKMT"
     primer_bind     complement(4682..4698)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      4701..4948
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(4967..4985)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4995..5011)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5156..5611
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5911..6315
                     /label=LEU2 promoter
     CDS             6328..7419
                     /codon_start=1
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
       61 ggacggatcg cttgcctgta acttacacgc gcctcgtatc ttttaatgat ggaataattt
      121 gggaatttac tctgtgttta tttattttta tgttttgtat ttggatttta gaaagtaaat
      181 aaagaaggta gaagagttac ggaatgaaga aaaaaaaata aacaaaggtt taaaaaattt
      241 caacaaaaag cgtactttac atatatattt attagacaag aaaagcagat taaatagata
      301 tacattcgat taacgataag taaaatgtaa aatcacagga ttttcgtgtg tggtcttcta
      361 cacagacaag atgaaacaat tcggcattaa tacctgagag caggaagagc aagataaaag
      421 gtagtatttg ttggcgatcc ccctagagtc ttttacatct tcggaaaaca aaaactattt
      481 tttctttaat ttcttttttt actttctatt tttaatttat atatttatat taaaaaattt
      541 aaattataat tatttttata gcacgtgatg aaaaggaccc aggtggcact tttcggggaa
      601 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      661 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
      721 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
      781 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
      841 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
      901 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
      961 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     1021 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     1081 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     1141 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     1201 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     1261 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     1321 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     1381 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     1441 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     1501 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     1561 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     1621 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     1681 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1741 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1801 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1861 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     1921 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1981 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     2041 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     2101 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2161 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2221 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     2281 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     2341 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     2401 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     2461 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     2521 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     2581 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttac ctcactcatt
     2641 aggcacccca ggctttacac tttatgcttc cggctcctat gttgtgtgga attgtgagcg
     2701 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     2761 ctcactaaag ggaacaaaag ctggagctct agtacggatt agaagccgcc gagcgggtga
     2821 cagccctccg aaggaagact ctcctccgtg cgtcctcgtc ttcaccggtc gcgttcctga
     2881 aacgcagatg tgcctcgcgc cgcactgctc cgaacaataa agattctaca atactagctt
     2941 ttatggttat gaagaggaaa aattggcagt aacctggccc cacaaacctt caaatgaacg
     3001 aatcaaatta acaaccatag gatgataatg cgattagttt tttagcctta tttctggggt
     3061 aattaatcag cgaagcgatg atttttgatc tattaacaga tatataaatg caaaaactgc
     3121 ataaccactt taactaatac tttcaacatt ttcggtttgt attacttctt attcaaatgt
     3181 aataaaagta tcaacaaaaa attgttaata tacctctata ctttaacgtc aaggagaaaa
     3241 aaccccggat tctagaacta gtatgcaggg ttctgctgct agtggtgaac aaaagttgat
     3301 ttctgaagaa gatttgaacg gtgaacaaaa gctaatctcc gaggaagact tgaacggtga
     3361 acaaaaatta atctcagaag aagacttgaa cggatcctct agaggtgaac aaaagttgat
     3421 ttctgaagaa gatttgaacg gtgaacaaaa gctaatctcc gaggaagact tgaacggtga
     3481 acaaaaatta atctcagaag aagacttgaa cggatcctct agaggtgaac aaaagttgat
     3541 ttctgaagaa gatttgaacg gtgaacaaaa gctaatctcc gaggaagact tgaacggtga
     3601 acaaaaatta atctcagaag aagacttgaa cggatccact ctacaggagt ctaggggatc
     3661 ctgtgtgcgt atcctcaagc agttccacaa ggacttagaa agggagctgc tccggcggca
     3721 ccaccggtca aagacccccc ggcacctgga cccaagcttg gccaactacc tggtgcagaa
     3781 ggccaagcag aggcgggcgc tccgtcgctg ggagcaggag ctaaatgcca agcgcagcca
     3841 tctgggacgc atcactgtag agaatgaggt ggacctggac ggccctcctc gggccttcgt
     3901 gtacatcaat gagtaccgtg ttggtgaggg catcaccctc aaccaggtgg ctgtgggctg
     3961 cgagtgccag gactgtctgt gggcacccac tggaggctgc tgcccggggg cgtcactgca
     4021 caagtttgcc tacaatgacc agggccaggt gcggcttcga gccgggctgc ccatctacga
     4081 gtgcaactcc cgctgccgct gcggctatga ctgcccaaat cgtgtggtac agaagggtat
     4141 ccgatatgac ctctgcatct tccgcacgga tgatgggcgt ggctggggcg tccgcaccct
     4201 ggagaagatt cgcaagaaca gcttcgtcat ggagtacgtg ggagagatca ttacctcaga
     4261 ggaggcagag cggcggggcc agatctacga ccgtcagggc gccacctacc tctttgacct
     4321 ggactacgtg gaggacgtgt acaccgtgga tgccgcctac tatggcaaca tctcccgctt
     4381 tgtcaaccac agttgtgacc ccaacctaca ggtgtacaac gtcttcatag acaaccttga
     4441 cgagcggctg ccccgcatcg ctttctttgc cacaagaacc atccgggcag gcgaggagct
     4501 gacctttgat tacaacatgc aagtggaccc cgtggacatg gagagcaccc gcatggactc
     4561 caactttggc ctggctgggc tccctggctc ccctaagaag cgggtccgta ttgaatgcaa
     4621 gtgtgggact gagtcctgcc gcaaatacct cttccctgca ggaattcgat atcaagctta
     4681 tcgataccgt cgacctcgag tcatgtaatt agttatgtca cgcttacatt cacgccctcc
     4741 ccccacatcc gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat
     4801 ttattttttt atagttatgt tagtattaag aacgttattt atatttcaaa tttttctttt
     4861 ttttctgtac agacgcgtgt acgcatgtaa cattatactg aaaaccttgc ttgagaaggt
     4921 tttgggacgc tcgaaggctt taatttgcgg ccggtaccca attcgcccta tagtgagtcg
     4981 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     5041 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     5101 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcgacgcg
     5161 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     5221 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5281 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5341 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5401 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5461 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     5521 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5581 aattttaaca aaatattaac gtttacaatt tcctgatgcg gtattttctc cttacgcatc
     5641 tgtgcggtat ttcacaccgc atatcgacgg tcgaggagaa cttctagtat atccacatac
     5701 ctaatattat tgccttatta aaaatggaat cccaacaatt acatcaaaat ccacattctc
     5761 ttcaaaatca attgtcctgt acttccttgt tcatgtgtgt tcaaaaacgt tatatttata
     5821 ggataattat actctatttc tcaacaagta attggttgtt tggccgagcg gtctaaggcg
     5881 cctgattcaa gaaatatctt gaccgcagtt aactgtggga atactcaggt atcgtaagat
     5941 gcaagagttc gaatctctta gcaaccatta tttttttcct caacataacg agaacacaca
     6001 ggggcgctat cgcacagaat caaattcgat gactggaaat tttttgttaa tttcagaggt
     6061 cgcctgacgc atataccttt ttcaactgaa aaattgggag aaaaaggaaa ggtgagaggc
     6121 cggaaccggc ttttcatata gaatagagaa gcgttcatga ctaaatgctt gcatcacaat
     6181 acttgaagtt gacaatatta tttaaggacc tattgttttt tccaataggt ggttagcaat
     6241 cgtcttactt tctaactttt cttacctttt acatttcagc aatatatata tatatttcaa
     6301 ggatatacca ttctaatgtc tgcccctatg tctgccccta agaagatcgt cgttttgcca
     6361 ggtgaccacg ttggtcaaga aatcacagcc gaagccatta aggttcttaa agctatttct
     6421 gatgttcgtt ccaatgtcaa gttcgatttc gaaaatcatt taattggtgg tgctgctatc
     6481 gatgctacag gtgtcccact tccagatgag gcgctggaag cctccaagaa ggttgatgcc
     6541 gttttgttag gtgctgtggg tggtcctaaa tggggtaccg gtagtgttag acctgaacaa
     6601 ggtttactaa aaatccgtaa agaacttcaa ttgtacgcca acttaagacc atgtaacttt
     6661 gcatccgact ctcttttaga cttatctcca atcaagccac aatttgctaa aggtactgac
     6721 ttcgttgttg tcagagaatt agtgggaggt atttactttg gtaagagaaa ggaagacgat
     6781 ggtgatggtg tcgcttggga tagtgaacaa tacaccgttc cagaagtgca aagaatcaca
     6841 agaatggccg ctttcatggc cctacaacat gagccaccat tgcctatttg gtccttggat
     6901 aaagctaatg ttttggcctc ttcaagatta tggagaaaaa ctgtggagga aaccatcaag
     6961 aacgaattcc ctacattgaa ggttcaacat caattgattg attctgccgc catgatccta
     7021 gttaagaacc caacccacct aaatggtatt ataatcacca gcaacatgtt tggtgatatc
     7081 atctccgatg aagcctccgt tatcccaggt tccttgggtt tgttgccatc tgcgtccttg
     7141 gcctctttgc cagacaagaa caccgcattt ggtttgtacg aaccatgcca cggttctgct
     7201 ccagatttgc caaagaataa ggttgaccct atcgccacta tcttgtctgc tgcaatgatg
     7261 ttgaaattgt cattgaactt gcctgaagaa ggtaaggcca ttgaagatgc agttaaaaag
     7321 gttttggatg caggtatcag aactggtgat ttaggtggtt ccaacagtac caccgaagtc
     7381 ggtgatgctg tcgccgaaga agttaagaaa atccttgctt aaaaagattc tcttttttta
     7441 tgatatttgt acataaactt tataaatgaa attcataata gaaacgacac gaaattacaa
     7501 aatggaatat gttcataggg tagacgaaac tatatacgca atctacatac atttatcaag
     7561 aaggagaaaa aggaggatag taaaggaata caggtaagca aattgatact aatggctcaa
     7621 cgtgataagg aaaaagaatt gcactttaac attaatattg acaaggagga gggcaccaca
     7681 caaaaagtta ggtgtaacag aaaatcatga aactacgatt cctaatttga tattggagga
     7741 ttttctctaa aaaaaaaaaa atacaacaaa taaaaaacac tcaatgacct gaccatttga
     7801 tggagtttaa gtcaatacct tcttgaacca tttcccataa tggtgaaagt tccctcaaga
     7861 attttactct gtcagaaacg gccttacgac gtagtcgata tggtgcactc tcagtacaat
     7921 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     7981 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     8041 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacg
//
