LOCUS       V008481                 8172 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008481
VERSION     V008481
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8172)
  AUTHORS   Kraft C.
  TITLE     A toolbox for the analysis of weak and low abundant molecule
            interactions in vivo by M-Track in budding yeast
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8172)
  AUTHORS   Brezovich A, Schuschnig M, Ammerer G, Kraft C.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2014) Department of Biochemistry and Cell Biology,
            MFPL, University of Vienna, Dr. Bohr Gasse 9, Vienna 1030, Austria
REFERENCE   3  (bases 1 to 8172)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8172)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-AUG-2014) Department of Biochemistry and Cell Biology, MFPL,
            University of Vienna, Dr. Bohr Gasse 9, Vienna 1030, Austria"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        135..239
                     /label="AmpR promoter"
     CDS             240..1097
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1271..1859
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2147..2168
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2183..2213
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2221..2237
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2245..2261
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2282..2300
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             2987..4486
                     /gene="ATG18"
                     /label="Autophagy-related protein 18"
                     /note="Autophagy-related protein 18 from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P43601"
     CDS             4547..4567
                     /label="TEV site"
                     /note="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
     CDS             4598..4771
                     /label="ProtA"
                     /note="IgG-binding unit of Staphylococcus aureus protein A"
     CDS             5330..5419
                     /label="3xHA"
                     /note="three tandem HA epitope tags"
     terminator      5441..5688
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     promoter        complement(5707..5725)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5735..5751)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      5892..6347
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(6481..7281)
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(7282..7497)
                     /label="URA3 promoter"
     misc_feature    complement(join(7767..8172,1..98))
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
ORIGIN
        1 aaactatttt ttctttaatt tcttttttta ctttctattt ttaatttata tatttatatt
       61 aaaaaattta aattataatt atttttatag cacgtgatga aaaggaccca ggtggcactt
      121 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
      181 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      241 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
      301 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
      361 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
      421 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
      481 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
      541 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
      601 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
      661 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
      721 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
      781 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
      841 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
      901 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
      961 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     1021 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     1081 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     1141 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     1201 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     1261 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1321 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1381 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     1441 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     1501 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     1561 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1621 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     1681 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     1741 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     1801 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     1861 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     1921 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     1981 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     2041 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     2101 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttacc
     2161 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcctatg ttgtgtggaa
     2221 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     2281 caattaaccc tcactaaagg gaacaaaagc tggagctcca ccgcggtggc ggccgctaac
     2341 gtagttatcc gaatgcataa attagactag tatgtgacaa cgctaaacat gcgttcccca
     2401 gaaccacctg cggttgttga gaggaatagg ttttgcgctt gtctatggac actttcttga
     2461 actcaatcca actgagcaat acttaacaat attgcgatta cttgttctta cataaagagt
     2521 aagaaatact tgcgaaataa acggtaatca atcaaattta gggtcataag ttgtattcgc
     2581 acaatatgca taagcactga ctcaagaaag aagatggttt tcgaatgggg agctagcaaa
     2641 aagagtcacc cctaagtata aaaactgaat tacaaagctc ttcgactact ttctgcaagt
     2701 aaatcattcg gaagtgcgac aatataatga caaaagacga taagttactt ctattaattg
     2761 aactttaagt atttggcttt tatttttttt ttttcatttt cttactcgcg cttttcttta
     2821 ttaagggctt tctcaattag atgttaataa tgtcataagg catcgttatt ccgctttata
     2881 ttggtaactc agcagtggtc attgccttcg ttcacgaccc tcccttatta atcagttagt
     2941 aatagtgttc cagttaactc tgtatccttt tctcttcggc ctgacaatgt ctgattcatc
     3001 acctactatc aactttatta atttcaatca aaccggaacg tgtatttccc ttggaacgtc
     3061 gaaaggtttc aaaatattca attgtgagcc cttcggaaaa ttttattcag aggacagtgg
     3121 gggctatgct atcgtcgaga tgttgttctc cacctcgtta ctagccctcg ttgggatagg
     3181 cgatcaacct gcgctttcac caaggagatt gcgtataatc aacacaaaaa aacattctat
     3241 tatctgtgag gtgactttcc ctacttctat tctgagtgtg aaaatgaata agtctcgatt
     3301 ggtggtactt ttacaagagc agatttatat ttatgatatc aacaccatga gactattgca
     3361 tactatagaa acaaacccta acccacgtgg ccttatggct atgtctcctt cggtagccaa
     3421 cagctattta gtgtatccat caccaccaaa agttattaac tccgaaatta aagctcatgc
     3481 caccacaaac aatatcacat tgtcagttgg tggcaacaca gagaccagtt tcaagagaga
     3541 tcagcaagat gctggccata gtgacattag cgacttggat cagtattcga gctttactaa
     3601 gagggatgat gcggatccaa caagcagtaa cggcggtaac agcagtataa taaagaatgg
     3661 tgatgtgatc gtattcaact tggaaacatt acagccaacc atggtcatcg aagctcataa
     3721 gggcgagatt gctgcaatgg caattagttt tgatgggaca ctaatggcta ccgcctctga
     3781 taaaggtact atcatcaggg tctttgacat tgaaacgggt gataagatct accaattcag
     3841 gagagggacg tacgcgacaa gaatttactc catatcattc agtgaagata gccagtactt
     3901 ggcggttacc ggctcttcca aaaccgtgca tatcttcaaa ttggggcatt caatgagcaa
     3961 caataaacta gacagcgatg atagcaacat ggaagaagct gcagccgatg attcatcgct
     4021 cgataccacc agtatcgatg cgctgagtga cgaggaaaac ccgacaagac tcgcaagaga
     4081 accatatgtg gatgcatcaa gaaagacaat gggtaggatg atacgttact cttctcaaaa
     4141 gctatcccga agagctgcca gaacattggg tcagattttc cccatcaaag ttacatcgtt
     4201 gttggaatcc tcgcgccatt ttgcgtcttt gaaacttccc gttgaaacca attcccatgt
     4261 aatgaccata tcaagtatag gctctccaat agatatagac acatccgagt atccggaact
     4321 cttcgaaact ggcaattccg caagtacaga gtcctaccat gagcctgtta tgaagatggt
     4381 ccccatcagg gtcgtttcct cggatggata cctatacaac tttgttatgg acccggagag
     4441 aggcggcgat tgcttaatat tgtcacagta ttccatcttg atggacctgc aggagaaaat
     4501 ctcatcctcc ggggcacttg attatgatat tccaactact gctagcgaga atttgtattt
     4561 tcagggagaa ttcggccttg cgcaacacga tgaagccgtg gacaacaaat tcaacaaaga
     4621 acaacaaaac gcgttctatg agatcttaca tttacctaac ttaaacgaag aacaacgaaa
     4681 cgccttcatc caaagtttaa aagatgaccc aagccaaagc gctaaccttt tagcagaagc
     4741 taaaaagcta aatgatgctc aggcgccgaa agtagacaac aaattcaaca aagaacaaca
     4801 aaacgcgttc tatgagatct tacatttacc taacttaaac gaagaacaac gaaacgcctt
     4861 catccaaagt ttaaaagatg acccaagcca aagcgctaac cttttagcag aagctaaaaa
     4921 gctaaatgat gctcaggcgc cgaaagtaga cgcgaatcat cagctgcagg acgacctcga
     4981 gtctagggga tccccgggga tcccgaattc ggctcgtact aagcagaccg ctcgcaagtc
     5041 caccggcggc aaggccccgc gcaagcagct ggcccagatc ccgaattcgg ctcgtactaa
     5101 gcagaccgct cgcaagtcca ccggcggcaa ggccccgcgc aagcagctgg cccagatccc
     5161 gaattcggct cgtactaagc agaccgctcg caagtccacc ggcggcaagg ccccgcgcaa
     5221 gcagctggcc cagatcccga attcggctcg tactaagcag accgctcgca agtccaccgg
     5281 cggcaaggcc ccgcgcaagc agctggccca gatctgcgga cgcatctttt acccatacga
     5341 tgttcctgac tatgcgggct atccctatga cgtcccggac tatgcaggat cctatccata
     5401 tgacgttcca gattacgctg ctcagtgcgt cgacctcgag tcatgtaatt agttatgtca
     5461 cgcttacatt cacgccctcc ccccacatcc gctctaaccg aaaaggaagg agttagacaa
     5521 cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag aacgttattt
     5581 atatttcaaa tttttctttt ttttctgtac agacgcgtgt acgcatgtaa cattatactg
     5641 aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt taatttgcgg ccggtaccca
     5701 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     5761 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     5821 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     5881 atggcgaatg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     5941 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     6001 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     6061 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     6121 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     6181 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     6241 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     6301 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcct gatgcggtat
     6361 tttctcctta cgcatctgtg cggtatttca caccgcatag ggtaataact gatataatta
     6421 aattgaagct ctaatttgtg agtttagtat acatgcattt acttataata cagtttttta
     6481 gttttgctgg ccgcatcttc tcaaatatgc ttcccagcct gcttttctgt aacgttcacc
     6541 ctctacctta gcatcccttc cctttgcaaa tagtcctctt ccaacaataa taatgtcaga
     6601 tcctgtagag accacatcat ccacggttct atactgttga cccaatgcgt ctcccttgtc
     6661 atctaaaccc acaccgggtg tcataatcaa ccaatcgtaa ccttcatctc ttccacccat
     6721 gtctctttga gcaataaagc cgataacaaa atctttgtcg ctcttcgcaa tgtcaacagt
     6781 acccttagta tattctccag tagataggga gcccttgcat gacaattctg ctaacatcaa
     6841 aaggcctcta ggttcctttg ttacttcttc tgccgcctgc ttcaaaccgc taacaatacc
     6901 tgggcccacc acaccgtgtg cattcgtaat gtctgcccat tctgctattc tgtatacacc
     6961 cgcagagtac tgcaatttga ctgtattacc aatgtcagca aattttctgt cttcgaagag
     7021 taaaaaattg tacttggcgg ataatgcctt tagcggctta actgtgccct ccatggaaaa
     7081 atcagtcaag atatccacat gtgtttttag taaacaaatt ttgggaccta atgcttcaac
     7141 taactccagt aattccttgg tggtacgaac atccaatgaa gcacacaagt ttgtttgctt
     7201 ttcgtgcatg atattaaata gcttggcagc aacaggacta ggatgagtag cagcacgttc
     7261 cttatatgta gctttcgaca tgatttatct tcgtttcctg catgtttttg ttctgtgcag
     7321 ttgggttaag aatactgggc aatttcatgt ttcttcaaca ctacatatgc gtatatatac
     7381 caatctaagt ctgtgctcct tccttcgttc ttccttctgt tcggagatta ccgaatcaaa
     7441 aaaatttcaa agaaaccgaa atcaaaaaaa agaataaaaa aaaaatgatg aattgaattg
     7501 aaaagctgtg gtatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     7561 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     7621 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     7681 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     7741 catgataata atggtttctt aggacggatc gcttgcctgt aacttacacg cgcctcgtat
     7801 cttttaatga tggaataatt tgggaattta ctctgtgttt atttattttt atgttttgta
     7861 tttggatttt agaaagtaaa taaagaaggt agaagagtta cggaatgaag aaaaaaaaat
     7921 aaacaaaggt ttaaaaaatt tcaacaaaaa gcgtacttta catatatatt tattagacaa
     7981 gaaaagcaga ttaaatagat atacattcga ttaacgataa gtaaaatgta aaatcacagg
     8041 attttcgtgt gtggtcttct acacagacaa gatgaaacaa ttcggcatta atacctgaga
     8101 gcaggaagag caagataaaa ggtagtattt gttggcgatc cccctagagt cttttacatc
     8161 ttcggaaaac aa
//
